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Jurnal Sains Teh dan Kina
Published by Pusat Penelitian Teh dan Kina
ISSN : -     EISSN : 29622034     DOI : -
Core Subject : Science, Agriculture, Social, Engineering,
Agriculture, Biological Sciences & Forestry Computer Science & IT Environmental Science Industrial & Manufacturing Engineering Mechanical Engineering
Jurnal Penelitian Teh dan Kina is a national journal providing rapid publication of peer-reviewed articles concerned with tea and cinchona commodities based on the aspects, agronomy, plant breeding, soil science, crop protection, postharvest technology and social economy. Papers dealing with result of original research on the above aspects are welcomed with no page charge.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 226 Documents
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The effect of benzyl amino purin (BAP) and indole butyric acid (IBA) concentrations on the growth of shoot and root of Cinchona ledgeriana Moens in vitro propagation Joko Santoso
Jurnal Penelitian Teh dan Kina Vol 15 No 1 (2012)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v15i1.20

Abstract

A research to evaluate effect of concentration of BAP and IBA on growth of shoot and root of Cinchona ledgeriana Moens in vitro culture, was conducted at Biomolecullar Laboratory of Research Institute for Biotechnology of Plantation Crops, Bogor. Testing on the effect of  IBA on shoot growth was conducted in a CRD with five treatments and replicated 15 times. The treatments were BAP at several concentrations: 1, 2, 3, 4, and 5 mg/l. MS media culture was used as basic media and BAP added were based on the concentration tested. The parameters measured were number of sproute explants, number of root and height of shoot. The effect of  IBA on the growth root was conducted based in a CRD with five treatments and replicated 15 times. The treatments were IBA at several con­centrations: 0,5; 1,0; 1,5; 2,0, and 2,5 mg/l. B5 media culture was used as basic media and IBA were added based on the concentration tested. The parameters measured were number of rooted plantlets, number of root and length of root. Result showed that: (1) the best effect of BAP on the inisiation of shoot growth was obtained from the treatment of 3 mg/l concentration of BAP wich sprouted at seven days. Shoot inisiation of all concentrations were occured at 14 days; (2) the best effect of BAP on number of sproute explants and  shoot height were obtained from the treatment of 3 mg/l concentration of BAP; (3) at seven days after cultured, the treatments of 1,5; 2,0; and 2,5 mg/l consentration of IBA gave the better root inisiation. At 14 days, the better root inisiation were obtained from the treatments of 1,5 and 2,0 mg/l consentration of IBA; (4) the treatment of 2,0 mg/l consentration of IBA gave the best effect on the number as well as the length of main root.
Analysis of leaves morphology similarity of several first generation tea clones Bambang Sriyadi
Jurnal Penelitian Teh dan Kina Vol 15 No 2 (2012)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v15i2.21

Abstract

As an effort to conserve tea clone accessions of first generation from genetic erosion, arrangement germplasm in one particular block/area to be easily monitored and managed, is needed. Ideally, tea germplasm collection should consist of a few clone accession number but high genetic variability. To select representative clones that will be conserved in new germplasm collection, leaves morphology similarity has been analysed by cluster analysis using average method. The results showed that by using leaves morphology parameter, from 50 tea clone accession of first generation coming from several tea plantations, it could be reduced into 25 clone accessions from seven clusters, which each has high similarity. The first cluster with 60% similarity could reduced accession from 15 to 6 clone. The second with 74,8% similarity from 25 to 13 clones. The third with 74,8% similarity, from 4 to 2 clones. The fourth with 61,3% similarity, and seventh with 82,1% similarity, both could reduce from 2 clones to 1 clone. To design a tea germplasm collection with 50 accessions of genetic variability, it could represented by 25 clones with low similarity, i.e PG 3, KP 1, Skm 18, Cin 69, Cin 67 dan Cin 58 as the first cluster representatives, Mel 101, PG 4, Pam 15, PG 5,  PG 11, GP 2, BD 13, SA 49, Cin 66, Mal 16, Bks 6 and Sin 5 the second cluster representatives;   Mel 108 and Cin 13 as the third cluster representatives; and Cin 15, Cip 37, GH 5, as well as GP 4 as the fourth, the fifth, and the sixth representatives, respectively.
The effect of NPK compound fertilizer (27%:6%:10%) compared to conventional fertilizers on productive tea plants of GMB 7 clone in the Andisols soil type Pudjo Rahardjo; Yati Rachmiati; Arkat Agus Salim
Jurnal Penelitian Teh dan Kina Vol 15 No 2 (2012)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v15i2.22

Abstract

The effectiveness application of compound fertilizer NPK (27%:6%:10%) to increase  tea shoots had been tested in Experimental Garden of Research Institute for Tea and Cinchona Gambung, Bandung, West Java, from January 2006 to March 2007. Experi­mental design used was randomized block design with 6 treatments with five replication. The plot sizes were 20 x 20 m = 400 m2/plot. The tea clone tes­ted was GMB 7. The appli­cation of compound fertilizer NPK (27%:6%:10%) were twice a year (50%-50%) and conventional fertlization were 4 times a year (20%-30%-20%-30%). The composition of  treatment was as follows: (A) 500 kg, (B) 600 kg, (C) 700 kg, (D) 800 kg/ha/year, and (E) conventional fertilizer recommended dosage of the Research Institute  for Tea and Cinchona Gambung (480 kg urea + 357 kg ZA + 260 kg SP 36 + 300 kg KCl + 278 kg  kieserit/ha/year), and (F) control (no fertilizer treatment). Parameters  observed were: 1. soil and leaf analysis at before and after treatment, 2. weighting the potential and the yield components each time of plucking, and 3. technoeconomic analysis. The results showed that: statistical analysis of the components as well as potential yield, and nutrient analysis (N, P, K, Mg and Zn) of the mother leaves of GMB 7 clones and the soil demon­strated the significantly differences among the treatments of all the various doses of compound fertilizer NPK and conventional fertilizer application to control (no fertilizer treatment). Meanwhile, there were no significantly differences among the result of  the doses of compound fertilizer NPK, to the conventional fertilizer applications. Further­more, dose of compound fertilizer NPK (27%:6%:10%) for produc­tive tea plant was recommendated at 700 kg/ha rate and was equivalent to 189 kg N/ha/year or 63% of the dose of conventional fertilizer, but could provide equivalent result to conventional fertilizer application, and not only could save the buying cost of conventional fertilizer  (urea, ZA, TSP/SP-36, KCl / MOP and kieserit) but also the cost of application cost in the field.
The effectiveness of the formulation of botanical insecticide marigold (Tithonia diversifolia) on Empoasca flavescens, a main pest on tea plant Odih Sucherman
Jurnal Penelitian Teh dan Kina Vol 15 No 2 (2012)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v15i2.23

Abstract

A research to know the effectiveness of formulation of botanical insecticide marigold (Tithonia diversifolia) on E. flavescens has been carried out at a green house of Research Institute for Tea and Cinchona (RITC), as well as at Pasir Sarongge Experimental Garden of RITC, Cianjur, West Java, from September up to November 2011. At both experimental sites, the experimentations was designed in a randomized complete block (RCB) with  six treatments, replicated four times. Treatments tested were formulation of marigold extract 10% and 15%, each at doses of 0.5 and 1.0 l/ha, standard chemical insecticide, and control. All treatments were applied by spraying, using one litre-hand sprayer at green house, and using knapsack sprayer with spraying volume 300 l/ha in feld. At green house, parameter observed was mortality of E. flavescens. Mortality at formulation of marigold extract 15% (92%, in average) was higher than at formulation of marigold extract 10% (76%, in average) while in field were attack intensity of E. flavescens, tea shoot production, and phytotoxicity. Results showed that at green house, formulation of marigold extract 10% and 15% at doses of 0.5 dan 1.0 l/ha were effective to result in high mortality of E. flavescens. While in field the formulations at both doses were effective to suppress attack intensity of E. flavescens. However, the effectiveness of 1.0 l/ha doses was higher than 0.5 l/ha doses, which comparable with standard chemical insectiside treatment. The formulations at both doses indirectly also could increase shoot production, and did not cause phytotoxicity  on tea leaves/shoots.
Analysis of individual cathecin from green tea extracted and fractionated by column chromatography Dadan Rohdiana; Shabri Shabri
Jurnal Penelitian Teh dan Kina Vol 15 No 2 (2012)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v15i2.24

Abstract

A study determination of solvent and eluent on extraction process and fractination of individual cathecin especially epigallocathecin gallate (EGCG) by column chromato­graphy method. This study devided on two steps, i.e. determination of raw material, sol­vent, process optimation for extraction and fravtination of green tea extract, evapo­ration and drying. Characterization of polyphenol conducted by visible spectro­photo­meter, analysis of individual cathecin by HPLC. This study was did in Laboratory of Processing Technology and Laboratory of Testing for Tea and Cinchona, Research Institute for Tea and Cinchona, at September 2007 up to March 2008. Result of this study shown that ratio of solvent aceton and water 7:3 v/v, ratio of tea and solvent 1:15 w/v for 15 minutes at 55-60ºC obtained 53.39% polyphenol on extract and yield gradiently produce extract with purity of cathecin is 94% b/b, EGCG 64.03% from feed with cathecin content is 82.96% w/w and EGCG 42.34% w/w.
Activity DPPH (1,1-diphenyl-2-picrylhidrazyl) free radical scavenging of some ready to drink teas on Dadan Rohdiana; Shabri Shabri
Jurnal Penelitian Teh dan Kina Vol 15 No 2 (2012)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v15i2.25

Abstract

Research of free radical scavenging activity of some ready to drink tea (RTD-Tea) was carried out. Generally, the result showed that tea beverages have a good of scavenging activity against DPPH free radical. T-AR was RTD-tea which has the stronger sca­venging activity against DPPH free radical. This RTD-tea has a low of EC50 value i.e. 63,55ml/ml. The scavenging activity followed were T-ES (63,95ml/ml), T-UP (65,75ml/ml), T-RO (86,85ml/ml), T-LI (91,15ml/ml), T-TA (107,53ml/ml), T-NG (125,36ml/ml) and T-AK (756,14ml/ml) respectively.
The effect of indigenous and exogeneous endophythic bacteria on shoot production, soil and tea leaf nitrogen content under drought season Eko Pranoto
Jurnal Penelitian Teh dan Kina Vol 17 No 1 (2014)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v17i1.27

Abstract

Drought season with rainfall <100 mm/month for more than two months cause tea plant growth disturbed, reduction in leaf pro­duction, and death of plant. Nitrogen was the macro essential nutrient needed for vegetative growth and was important for building the cell, include protein, DNA, and RNA. Nitrogen was related on proline content to support the drought tolerance in plant. The nitrogen re­quirement on tea plantation can be fulfilled by anorganic, organic, and biofertilizer. This study was aimed at determining effect of indigenous and exogeneous endophythic bacteria effec­tiveness on productivity and nitrogen content on soil and tea plant. This research was carried out at the Block A7 Gambung, Research Intitute for Tea and Cinchona. The study was conduc­ted during drought season (June to September 2013). The experimental design was randomize blocked design with factorial arrangement and three levels of each factor: i.e. 1. anorganic nitrogen dose (N): a. 100% N dose; b. 75% N dose; c. 50% N dose, 2. endophytic bacteria (E); a. control (without); b. indigenous (DtG7-5); c. exogenous (Acinetobacter sp). The result showed that the indigenous and exogenous endophytic bacteria could be maintained the leaf production and significantly different on drought season, i.e. on 3rd, 4th, and 6th plucking. The highest leaf production increasing depend on before applied the treatment was the combined of endophitic bacteria and 100% anorganic fertilizer. All of the treatment has not significantly different on N-total of soil and N content of plant. The applied of indigenous and also exogenous endophytic bacteria was pro­duced the average of nitrogen content on soil around 10-13% higher than the control treat­ment (only anorganic fertilizer applied) and the nitrogen content on plant was lower 1-2% than control treatment (only anorganic fertilizer applied).
In-vitro experiment of nitrogen fixing capacity of indigenous and exogeneous Azotobacter sp at Andisol tea planting area’s Eko Pranoto; Mieke Rochimi Setiawati
Jurnal Penelitian Teh dan Kina Vol 17 No 1 (2014)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v17i1.43

Abstract

An in-vitro experiment to evaluate total nitrogen content of soil of tea plantation area included with indigenous and exogenous Azoto­bacter sp have been conducted in the Biology and Soil Biotechnology Laboratory, Padja­djaran University. A completely randomized design with 11 treatments and three repli­cations was used. The treatments were: A (ne­gative control); B (positive control); C (A. Kedelai II); D (A. vinelandii); E (A. N.D.9.3); F (A. Padi); G (VI-1); H (II-1); I (V-2); J (I-1); and K (III-2). Size of microbe inoculated popu­lation for each treatment was 1,2 x 1011 CFU/ml. One percent dose was used for ste­rilized soil originated from the tea plantation area. After five days of incubation period re­vealed that II-1 Azotobacter sp produced very significantly different in total nitrogen pro­duction than the other Azotobacter sp, i.e. 0,8532%. Orthogonal comparisons indicated that indigenous Azotobacter sp had the capacity of nitrogen fixation 7,67% higher than that the capacity of nitrogen fixation of exogenous Azotobacter sp for growth on Andisol soil of tea plantation area with pH of 4,5-5,6. Although, Azotobacter sp is very rarely found in soil with low pH (high acidity), this phenomena indi­cating that an active biofertilizer of indigenous Azotobacter sp is present in tea plantation area.
The effect of pottasium (K) on the development of scarlet mite (Brevipalpus phoenicis Geijske) population at tea crop (Camellia sinensis (L.) O. Kuntze) Odih Sucherman
Jurnal Penelitian Teh dan Kina Vol 17 No 1 (2014)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v17i1.44

Abstract

A research experiment to know the effect of pottasium on the population development of scarlet mite and growth of young tea was carried out. The experiment was conducted at Green House of Plant Protection Department Research Institute Tea and Cinchona Gam­bung, Bandung, West Java, from December 2011 until July 2012. The soil type used is Andisol and altitude is 1.250 m above sea level. The treatments were potassium fertilizer appli­cations of 0, 30, 40 (standars treatment), 50, 60, 70, 80, 90, and 100 kg K20/ha/year; in which the 0 dosage was used as untreated control. The result of the experiment showed that: pottasium fertilizer at the dosage 70 kg K2O/ha/year to 100 kg K2O/ha/year reasonably effective in pressuring the development of the population development of scarlet mite and increasing the number of leaves as indication of rising class seedlings on young tea. Pottasium fertilizer at the medium dosage 70 kg K2O/ha­/year reasonably effective in pressuring the development of the population development of scarlet mite and increasing the number of leaves as indication of rising class seedlings, when compared to the standards (40 kg/ha/y) and control dose on young tea.
Synthesis of quinine N-oxide and an NMR tutorial in its structure determination Aisyah Aisyah; Nila Berghuis Tamaela; Joko Santoso; Yana Maolana Syah; Didin Mujahidin
Jurnal Penelitian Teh dan Kina Vol 17 No 1 (2014)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v17i1.45

Abstract

Cinchona alkaloids are one of abundant natural chiral pool for organocatalysts. The application of an N-oxide group containing molecule in asymmetric reaction is currently wide developed. The structure of quinine consist two amine groups that could be oxidized to the N-oxide groups at N-1’ of quinoline and at N-1 of quinuclidine moieties. The oxidation reaction took place selectively at quinuclidine moiety by controlling the concentration of oxidizing agents. In this study we showed that N-1-oxide quinine could be occurred chemo­selectively by using a low concentration of ozone thana palladium catalyzed oxidation. The structure of products N1-oxide quinine was elucidated by spectroscopy data including 1H-NMR, 13C-NMR, 2D-NMR, infra-red and mass spectrometry. The developed method is a chemoselectively and eco-friendly method for synthesis N1-oxide quinine.

Page 11 of 23 | Total Record : 226

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