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Activity DPPH (1,1-diphenyl-2-picrylhidrazyl) free radical scavenging of some ready to drink teas on Rohdiana, Dadan; Shabri, Shabri
Jurnal Penelitian Teh dan Kina Vol 15, No 2 (2012)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.698 KB) | DOI: 10.22302/pptk.jur.jptk.v15i2.25

Abstract

Research of free radical scavenging activity of some ready to drink tea (RTD-Tea) was carried out. Generally, the result showed that tea beverages have a good of scavenging activity against DPPH free radical. T-AR was RTD-tea which has the stronger sca­venging activity against DPPH free radical. This RTD-tea has a low of EC50 value i.e. 63,55ml/ml. The scavenging activity followed were T-ES (63,95ml/ml), T-UP (65,75ml/ml), T-RO (86,85ml/ml), T-LI (91,15ml/ml), T-TA (107,53ml/ml), T-NG (125,36ml/ml) and T-AK (756,14ml/ml) respectively.
Antibacteririal avtivities of Bacillus cereus and Shigella dysenteriae of white tea extract in several variation of solvents Widyasanti, Asri; Priantiwi, Andita Mega; Rohdiana, Dadan
Jurnal Penelitian Teh dan Kina Vol 19, No 1 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.542 KB) | DOI: 10.22302/pptk.jur.jptk.v19i1.81

Abstract

Tea is used in the food, cosmetic, and herbal drug industries. White tea is a kind of tea containing a relatively high polyphenol content compared to other kind of tea, and thus has the potential of being used as a natural antibacterial. The objective of this research was to study the antibacterial activity of of  white tea extract in several variation of  13 % (w/v) concentration of solvents to the activity of gram-positive bacteria (Bacillus cereus) and Gram-negative bacteria (Shigella dysenteriae). Laboratorium experimental method was used in this descriptive study. The antibacterial activity study was comprised of estimating inhibition area and total of bacterial growth. The results revealed that white tea extract inhibited bacterial growth, indicating that white tea extract from the n-hexane, ethyl acetate and 96% ethanol solvents could be developed as an antibacterial material. The diameter of the inhibition area to Bacillus cereus bacteria for n - hexane , ethyl acetate, and  ethanol 13 % (w/ v)  solvents were 0.6 mm, 10.5 mm and 1.75 mm, respectively; while for Shigella dysenteriae bacteria were 0,5 mm, 9,86 mm  and 10,6 mm, respectively. The total growth of Bacillus cereus bacteria for n-hexane, ethyl acetate, and ethanol 13 % (w/v) solvent were 1.3 x 107, 6.0 x 106, and  1.1 x 104 CFU / ml , and of Shigella dysenteriae bacteria were  1.0 x 107,  6.5 x 106 and 4.6 x 106 CFU / ml, respectively.
Optimization and characterization of green tea polyphenol extract from various solvents Shabri, Shabri; Rohdiana, Dadan
Jurnal Penelitian Teh dan Kina Vol 19, No 1 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.395 KB) | DOI: 10.22302/pptk.jur.jptk.v19i1.82

Abstract

Tea has a high content of polyphenols with potent antioxidant activity and beneficial for health. Availability of polyphenols extract is still very limited. The aim of this study to optimation and characterization of green tea polyphenol extract base on some solvents. Parameters analyzed were polyphenol content and yield of some extraction process conditions. This result showed that extraction by the ratio of tea and solvent was 1 : 15 b/v, extracted by acetone 70% solution in temperature 600C for 15 minutes obtain polyphenol content of 53,30% b/b and total yield of 40,17% b/b.
Antioxidant activity of ten grades of Indonesia black tea (Camellia sinensis (L.) O. Kuntze) liquor Sudaryat, Yayat; Kusmiyati, Mimin; Pelangi, Citra Ratu; Rustamsyah, A; Rohdiana, Dadan
Jurnal Penelitian Teh dan Kina Vol 18, No 2 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (290.86 KB) | DOI: 10.22302/pptk.jur.jptk.v18i2.70

Abstract

Tea is one kind of plant that is popular as a beverage. These plants contain antioxidant potential of flavonoids derived from polyphenol compounds. The research aimed to examine the antioxidant activity and to determine the con-tent of total phenols and total flavonoids from ten grades of Indonesian black tea liqour. Antioxidant activity was measured by the method of free radical scavenging by DPPH, total phenol content measured by Follin-Ciocalteu method, and total flavonoid content was measured by the method of AlCl3. The results of this research showed that The highest total phenol content contained in Dust III with 225,80 mgGAE/100 g and the lowest content is BTL with 111,26 mgGAE/100 g. The test results showed that BBL has the highest total flavonoid content with 0,151 mg/g, while the lowest is BTL flavonoid with 0,086 mg/g. Meanwhile the highest antioxidant activity of ten grade of Indonesian black tea infusion obtained by Dust I, with IC50 97,00 μg/ml. Meanwhile the lowest antioxidant activity with IC50 178,56 μg/ml was obtained by BTL.
Antibacterial activity of white tea extract against gram positive and negative bacteria Widyasanti, Asri; Hajar, Siti; Rohdiana, Dadan
Jurnal Penelitian Teh dan Kina Vol 18, No 1 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.785 KB) | DOI: 10.22302/pptk.jur.jptk.v18i1.66

Abstract

This research was carried out to deter­mine antibacterial activity of white tea extract against gram positive and gram negative bacteria based on its MIC (minimum inhibitory concentration). Ethanol solution 96% were used to extract of white tea with a ratio of 1:9 (w/v) by using maceration method. Paper disc diffusion method was used to determination of the MIC. The concentrations were used of 2%, 4%, 6%, 8%, 10%, and 12% (w/v). The results of this research showed that MIC of white tea extract of 4%, 6%, 6% and 10% (w/v) against Pseudomonas aeruginosa ATCC 9027, Staphy­lococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 12228, and Escherichia coli ATCC 25922, respectively. The inhibition zone for 4 bacteria were 10.83 mm, 10.08 mm, 10.16 mm and 12.08 mm respectively. This result of research concluded that white tea extract have antibacterial activity against both gram positive and gram negative bacteria
Production of fungal pectinase enzyme through solid substrate fermentation of estate waste for improvement of enzymatic oxidation and increasing the quality of CTC tea Panji, Tri; Suharyanto, Suharyanto; Shabri, Shabri; Rohdiana, Dadan; Yusianto, Yusianto
Jurnal Penelitian Teh dan Kina Vol 18, No 1 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.925 KB) | DOI: 10.22302/pptk.jur.jptk.v18i1.55

Abstract

Enzymes, especially pectinase, plays an important role in the processing of tea that determines the quality and the color appearance of black tea. The main obstacle encountered in the application of enzymes in food processing in general, and in particular in the processing of black tea is the price of commercial enzyme that are still very expen­sive, because it is still imported. Indonesia has a high fungal diversitythat is potential as producer of commercial pectinase enzyme. To reduce the cost of production, fermentation was done using solid waste of plantation that rich of pectin content such as cocoa pod husk that has not been optimally utilized. The research aimed to develop the production technology for pectinase enzymes of fungi through solid substrate fermentation for applications in the processing of CTC black tea. Research includes the isolation and selection of local superior fungi producing enzymes pectinase, optimiza­tion of enzyme production by fungi isolates were selected through a solid substrate fermen­tation using cocoa pod husk waste, production optimization included optimization of fermen­tation time and substrate composition, enzyme extraction of fermented substrate, and appli­cation of pectinase enzyme extracts in the processing of CTC black tea and sensory test of fermented tea. The results showed that it has been obtained isolates of Aspergillus niger on Pectinase Screening Agar Medium (PSAM) which was capable of producing pectinase enzyme on growing media of mix cocoa pod husk and rice bran with additional of enrichment media. The optimum conditions of pectinase production was obtained in the composition of cacao pod husk: rice bran (80:20 w/w) plus enrichment media, the incubation time of 9 days, which produced pectinase activity of 125 U/mL. Applications of pectinase on enzymatic oxidation of CTC tea proved that it was capable of raising the quality of the tea flavor, though the tea gradedid not change.
The decreasing activity of white tea and green tea on smokers lipids profile Kushargina, Rosyanne; Rimbawan, Rimbawan; Setiawan, Budi; Rohdiana, Dadan
Jurnal Penelitian Teh dan Kina Vol 18, No 1 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.889 KB) | DOI: 10.22302/pptk.jur.jptk.v18i1.68

Abstract

Smoking habit have many negative effect, including increased lipids profile. Researching on the effects of tea on lipids profile on smokers has been carried out using green tea. Research using white tea has been limited, therefore, this study aimed to analyze the effect of white tea than green tea on lipids profile in smokers, using paired sample of clinical trials design. The subjects were medium smokers (11-21 cigarettes/day) aged 30-45 years and have milddyslipidemia. Each subject was asked to drink tea in two times. Firstly, all subjects were asked to drink 3 x 200 ml white tea/day for 28 days. Secondly, treatment will be start after two weeks washout period, and they were asked to drink 3 x 200 ml of green tea/day for 28 days. Lipids profile (trigliserida (TG); cholesterol total (TC); low density lipoprotein-cholesterol (LDL-C)) of blood serum has been measured four times, at pre-post intervention of white tea and at pre-post intervention of green tea. The lipids profile were significantly decreased after white tea intervention (paired sample t-test, p<0,05). After green tea intervention,the lipids profile were decreased but significant (paired sample t-test, p<0,05) only for LDL-C. The independent sample t-test analysis showed that white tea has higher effect in decreasing the lipids profile greater than green tea, however not significantly different (p>0,05). This study was proved that white tea is more potential than green tea to decreasing lipids profile of smokers.
Antioxidant activity, phenol total, and flavonoid total of green tea (Camellia sinensis (L.) O. Kuntze) from three West Java tea estate Kusmiyati, Mimin; Sudaryat, Yayat; Lutfiah, Isti Agnia; Rustamsyah, Ardi; Rohdiana, Dadan
Jurnal Penelitian Teh dan Kina Vol 18, No 2 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.507 KB) | DOI: 10.22302/pptk.jur.jptk.v18i2.71

Abstract

Green tea has bioactive component, it is polyphenol which has strong antioxidant. As the largest component of polyphenol, flavonoid is very effective as an antioxidant. The purpose of this study was to determine antioxidant activity, phenol total and flavonoid total in green tea from three different estates, they were Cikajang Kab. Garut, Taraju Kab. Tasikmalaya, and Ciwidey Kab. Bandung. The results of this study show that seven samples have good antioxidant activity, but green tea which came from Cikajang (P-IRT No. 810320501698) has the highest IC50 grade it was 21,44 μg/ml. Beside that samples from Cikajang (P-IRT No. 810320501698) has highest total phenol content it was 334,68± SD 0,89/100 g samples and also has the highest total flavonoid it was 0,34 mg Kuersetin/g samples ± SD 0,21. On the contrary, green tea which has the lowest IC50 grade came from Taraju, the grade was 28,03 μg/ml, the samples has the lowest total phenol content it was 208,91± SD 4,47 mgGAE/100 g, and also has the lowest total flavonoid it was 0,207 mg Kuersetin/g samples ± SD 0,00.
Briskness index of black tea based on arrangement technique of brewing period Rohdiana, Dadan; Shabri, Shabri
Jurnal Penelitian Teh dan Kina Vol 17, No 2 (2014)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (36.725 KB) | DOI: 10.22302/pptk.jur.jptk.v17i2.49

Abstract

Research of briskness index of black tea base on arrangement technique brewing period has been done. The aim of this research is to find the best brewing periode arrangement technique which indicated the height of briskness index by determine of theaflavin and caffeine content. Result of this research shown that brewing periode arrangement for 12 minutes initiated brewing for 3 minutes has the highest of briskness index, i.e. 22.71 with theaflavin and caffeine content were 0,29% and 0,97% dry basis respectively. In contras, brewing periode arrangement technique for 3 minutes initiated by brewing periode 12 and 6 minutes respectively were liquor with the lowest of briskness index i.e. 13.73 with theaflavin and caffeine content were 0.10% and 0.66% dry basis respectively. This research concluded that black tea could be brewed until 3 times.
Inhibitory activity of Escherichia coli by type of teas and its liquors Rohdiana, Dadan; Arief, Dede Zainal; Budiman, Arista
Jurnal Penelitian Teh dan Kina Vol 16, No 1 (2013)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.671 KB) | DOI: 10.22302/pptk.jur.jptk.v16i1.86

Abstract

Study of analysis polyphenol content on tea, liquor, and its activity as antibacterial on Escherichia coli has been done conducted in Laboratory of Research, Department of Food Technology, Pasundan University, and Laboratory of Chemistry, Department of Chemistry, Faculty of Mathematic and Natural Science, Padjadjaran University. The result of this research showed that green tea sample has the highest polyphenol content, i.e. 23,18% followed orthodox and CTC (crushing tearing curling) black tea samples i.e. 14,23%  and 13,93% respectively. Activity of inhibitory on E. coli showed that ratio tea and water 2 : 50 w/v has the best inhibition i.e. 117,71 mm2.
Co-Authors A Rustamsyah A Rustamsyah, A Achmad Imron Rosyadi Adang Firmansyah Akhmad Endang Zainal Hasan Amalia Rahma Ananda, Marsha Radhia Andita Mega Priantiwi Andita Mega Priantiwi, Andita Mega Andri Agustav Wirabudi Angga Rusdinar Anik Setiawati Anik Setiawati, Anik Ardi Rustamsyah Ardi Rustamsyah Arista Budiman Arista Budiman, Arista Asep W. Perdana Asri Widyasanti Awaliah, Nisa Azmi, M. Haikal Beauty Azhary Bina Lohita Sari Budi Setiawan Cece Sumantri Citra Ratu Pelangi Citra Ratu Pelangi, Citra Ratu Clara Meliyanti Kusharto Darningsih, Sri Dede Zainal Arief, Dede Zainal DIMAS ANDRIANTO Dinda Nuraini Maulfida Dini Febrianty Dong Ho Lee Dwi Putra, Januar Hafiz Dwiyan Nugraha Dytha Andri Deswati, Dytha Andri Ekatama, Novriana Evy Damayanthi Faillah, Umi Lutfiatul Fani Fauziah Farddani, Chintya Listiarsi Fauzan, Fajar Akmal Fauzi, Ridho Hadi Riyadi Hanipah, Anida Hanny Hafiar Hasna Zakiyah Heddy Julistiono Heri Aryadi Hidayat, Taufik Septiyan Hijaya, Andi Hilman Maulana Hilman Maulana Hilman Maulana I Gusti Bagus Wiksuana Ida Maryam Ika D. Mayangsari Ikrawan, Yusep Isti Agnia Lutfiah Isti Agnia Lutfiah, Isti Agnia Komar Ruslan Wirasutisna Komar Ruslan Wirasutisna Kristin, Julia M Iqbal Prawira-Atmaja M. Iqbal Prawira-Atmaja Mamay Somantri Mamay Somantri, Mamay Marlinda Adham Martini, Rina Maryam, Ida Mimin Kusmiyati, Mimin Miranti Putri Utami Muhamad Alvin Adriyan Muhamad Insanu Muhammad Insanu Muhammad Iqbal Prawira-Atmaja Mukhammad Iqbal Prawira-Atmaja Mukhammad Iqbal Prawira-Atmaja Murdijati Gardjito Novi Susanti Nuni Yunita Nuni Yunita, Nuni Nurlita, Selvi Nurzanah, Fitri Prawira-Atmaja, Mukhammad Iqbal Rachmat Mauludin Rahayu, Dien Puji Rendy Munadi Rimbawan , Rimbawan Rimbawan Rina Martini Rohayati Suprihatini Rosialani, Resa Rosyanne Kushargina Rosyanne Kushargina Sahara, Putri Siti Sari Agustin Shabri Shabri Shabri Shabri Shabri Shabri Shabri Shabri Shabri Shabri, Shabri Siti Hajar Siti Hajar Sri Anna Marliyati Sri Raharjo SUGENG HARIANTO Sugeng Harianto Sugeng Harianto Sugeng Haryanto SUHARYANTO SUHARYANTO Syumillah Saepudin Tri Halimah Tri Panji Yayat Sudaryat Yunita Al-Azzahra Yusianto Yusianto Yusianto Yusianto, Yusianto Zahra, Nur Adni Azkia Zanil Musa