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Omni-Akuatika
ISSN : 18583873     EISSN : 24769347     DOI : -
OmniAquatika is a scholarly refereed research journal which aims to promote the theory and practice, innovation, engineering and management as well as social-economic relevant in fisheries and marine sciences fields. The main aspects of research areas include, but are not exclusive to the current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems in the field of fisheries and marine science. The journal also welcome other aquatic relevant aspect related to the transport, fate, control of nutrients and abatement of pollutants in the aquatic environment as well as the ecosystem rehabilitation. The authors and readers are students, scientists, and regulatory experts from the academic, industrial, and government sectors worldwide. Master thesis and part of dissertation research work might becoming the potential contributors. High-quality, research articles make up the primary content. Other contributions are short communications, reviews, and special issues. Before contributions are accepted for publication, they must pass a peer-review process managed by the editor-in-chief.
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Articles 290 Documents
The Effect of Different Thyroxine Hormone (T4) Concentration on The Growth, Survival, and Pigment Development of Pink Zebra Fish Larvae (Brachydanio reiro) Arifianto Heraedi; Slamet Budi Prayitno; Tristiana Yuniarti
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1240.104 KB) | DOI: 10.20884/1.oa.2018.14.2.538

Abstract

The thyroxin hormone plays an important role in the process of metabolism, yolk sac absorbsion, and growth of fish. The aims of this research were to observe the effect of various concentration of thyroxin (T4) on the absolute growth weight, total length, survival rate, and pigment development of pink zebra fish larvae (Brachydanio reiro ) after being reared 42 days. The pink zebra fish larvae at 4 day age with the average weight of  0,002 – 0,003g and average length of ± 3.10 – 3.43 mm were immersed at various concentration of T4 for 24 hours. Prior the treatment fish larvae were dipped into 1 ppt salinity for 2 minutes then transferred into 1 Liter, 1ppt saline and various T4 in plastic bags. The thyroxin concentrations were A (0 mg / L); B (0.05 mg / L); C (0.10 mg / L) and D (0.15 mg / L) respectively. The stocking density was 40 fish/L. After that they were transferred into aquariums and reared for 42 days. Completely Randomized Design (RAL) with four treatments and three replications were used. The variables observed were absolute and specific growth, total length, survival rate and hue degree. The results showed that the thyroxin hormone had significant effect on absolute growth weight, total length and specific growth rate.  The dosage 0.1 mg/L was the best treatment on absolute growth, total length, and specific growth rate. Whilee the survival rate showed no significant differences across the treatments. Treatment C also demonstrated the best ppigment development (14.60 + 0.36º hue) compared to others.
The Effects of Papain Enzyme Supplement in Feed on Protein Digestibility, Growth and Survival Rate in Sangkuriang Catfish (Clarias sp) Diana Rachmawati; Istiyanto Samidjan
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1121.758 KB) | DOI: 10.20884/1.oa.2018.14.2.551

Abstract

The study aimed to assess the effects of Papain Enzyme Supplement in Feed on Protein Digestibity, Growth and Survival Rate of  Sangkuriang Catfish (Clarias sp).  Fingerlings used in the study were acquired from the Center for Freshwater Hatchery and Aquaculture, Muntilan, Central Java, Indonesia. The fingerlings had average weight of 3.43±0.50 g/fish. Completely Randomized Design was used in the experiments with 5 (five) treatment and 3 (three) repetitions. The experimental feed contained 31% of protein and 252.06 Kcal/g of energy. Various doses of the addition of papain enzyme were incorporated into the feed, those doses were A (0 g/kg feed), B (2 g/kg feed), C (4 g/kg feed), D (6 g/kg feed) and E (8 g/kg feed).  Parameters of digestibility of protein (ADCP), relative growth rate (RGR), efficiency of feed utilization (EFU), feed conversion ratio (FCR), protein efficiency ratio (PER), survival rate (SR) and water quality were evaluated.  The papain enzyme supplement significantly (P<0.01) influenced on ADCp, RGR, EFU, FCR, and PER, otherwise SR of catfish was insignificantly affected by the papain enzyme supplement. The best treatment result was the addition of 6/kg feed papain enzyme (treatment D). The study concluded that the optimum dose of the papain enzyme supplement for ADCp, EFU, FCR, PER and RGR were 5.65, 5.62, 6.0, 5.66, 6.0  g/kg feed respectively. Water quality during study was still in favorable condition for nurturing Sangkuriang catfish (Clarias sp).  
Micro/Nano Bubble Technology : Characteristics and Implications Biology Performance of Koi Cyprinus carpio in Recirculation Aquaculture System (RAS) Henry Kasmanhadi Saputra; Kukuh Nirmala; Eddy Supriyono; Nurul Taufiqu Rochman
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.789 KB) | DOI: 10.20884/1.oa.2018.14.2.539

Abstract

Technology for aquaculture is growing with the high demand of fish. Micro / nano bubble is one of technology that is developed to increase dissolved oxygen in water. This technology is used to increase Koi fish production in high density via RAS. The purpose of this study was to determine the characteristics of micro / nano bubble technology and its effect on biology of koi fish (Cyprinus carpio). Lutor was used to introduce micro / nano bubble in the fish tank by using fish length (e.g. 7.49 ± 0.29 cm). This research used completely randomized design with one factor (fish density) and three replications. This factor was three densities of fish at 15 fish.60 L-1 (A), 30 fish.60 L -1 (B), and 45 fish. 60 L -1 (C). The result showed that there was the absolute length of each treatments was (0.52 ± 0.03a) cm for A, treatment  B (0.36 ± 0.07ab) cm and C treatment (0.29 ± 0.08b) cm. The best treatment is A treatment (15 fish. 60 L -1) and different significant in statistic with C treatment (45 fish. 60 L-1) but not significant in statistic with B treatment (30 fish.­ 60 L -1).
Characteristics of Gel Emulsion Formulation of Snakehead (Channa Micropeltes) As Wound Healer Firlianty Firlianty; Choirul Anwar; Anang Najamuddin; Silvester B. Pratasik
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.642 KB) | DOI: 10.20884/1.oa.2018.14.2.545

Abstract

This study is aimed to know the characteristics and the effectivity of gel emulsion of snakehead (Channa micropeltes) extract in accelerating wound healing process. It used experimental method with Complete Randomized Design. Four treatments with 3 replications were employed, i.e. 1%, 2%, and 4% gel emulsion of snakehead Channa micropeltes extract and a control treatment. Results showed that C. micropeltes extract formulated in gel emulsion had white colour, typical aroma of gel emulsion, soft texture with mean dispersion ability of 3 cm, and pH 6. In vivo test on male mices that gel emulsion of C. micropeltes extract was highly effective (P≤0.05) to accelerate wound healing, in which the treatment of 1% gel emulsion gave the highest effect with 98.5% wound healing at day-14, followed with that of control treatment, 95% healing, then 2%, 90% healing and 4%, 90% healing, respectively. Snakehead (Channa micrpeltes) extract formulated in gel emulsion can speed up the wound healing process.
The Diversity of Causative Agent Associated With Bacterial Diseases on Catfish (Clarias gariepinus) with Molecular Based from Demak, Indonesia Sarjito Sarjito; A. Harjuno Condro Haditomo; Desrina Desrina; Restiana Ariyati; S. Budi Prayitno
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.723 KB) | DOI: 10.20884/1.oa.2018.14.2.553

Abstract

Bacterial diseases is frequently occur in catfish culture. The aim of this research was to find out the diversity of causative agent associated with bacterial diseases in catfish based on 16S rDNA gene sequences. The combination between exploratory in the field and experiment, method were applied. Seventeen isolates (D01–D17) were gained from kidney and external wound of moribound catfish with NA and GSP medium that were collected from fish pond of Demak Regency, Indonesia. Based on the postulat results showed that four isolates (D07, D10, D11 and  D14) that  were  caused  10–55% of fishes get sick  and  0–30%  fishes mortal.  On the other hand, there were 13 isolates do not cause both sick and mortality of fish.  On the basis of sequence 16S rDNA analysis, the result showed that D07, D10, D11 and D14 were closely related to Aeromonas caviae (96%), Aeromonas veronii (97%.), Plesiomonas shigelloides (97%) and Pseudomonas putida (96%) respectively. The sensitivity test result indicated that these causative agents have not sensitively to some fish drugs test.
Study of Proportions Seaweed Eucheuma cottonii L. and Cooking Time on Quality of Functional of Seaweed Dodol David Hermawan; Warkoyo Warkoyo; Claudia Jasmine; Okta Pringga Pakpahan
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.825 KB) | DOI: 10.20884/1.oa.2018.14.2.540

Abstract

Seaweed as a source of nutrition contains carbohydrates, protein, lipid and ash. Ones of ingredients that works for healthy were dietary fiber. Eucheuma cottonii seaweed contains carrageenan, which can act as a stabilizer, a material thickening, gelling agent, and emulsifiers. This research is aim to know the level of resilience Dodol with the proportion of seaweed and cooking time. The study was conducted using a randomized block design consisting of two factors. The first factor is proportions seaweed thickener composition, glutinous rice flour, rice flour (30:35:30, 40:25:20, 50:15:10), the second factor is cooking time (20, 30, 40 minutes) repeated three times. The observations parameter includes moisture content, ash content, protein, lipid, crude fiber, and texture.The research results showed that has interaction between the proportion of seaweed and cooking time. The proportion of seaweed and cooking time gives effect to the moisture content and the texture of dodol seaweed. The proportions give effect to the moisture content, ash, lipid, crude fibre, and texture while treatment cooking time gives effect to the moisture content, ash, protein, lipid, and texture of dodol seaweed. The best treatment is produced by treatment of 50 g of seaweed: 15 g of glutinous rice: 10 g of rice flour and 40 minutes cooking time with the result 62.10% moisture content, 3.32% ash content, 2.42% protein, 3.25% lipid, 5.20% crude fibre, 76.70 N texture. 
Effectively Of 17α-Methyltestosterone on Tropical Eel, Anguilla bicolor McClelland Masculinization in Different Salinity Culture Farida Nur Rachmawati; Ridwan Affandi; Yulia Sistina
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.843 KB) | DOI: 10.20884/1.oa.2018.14.2.546

Abstract

Eel population in nature reaches critical number, so that culture strategy is urgently needed to fulfil the high demand of this fish. A shortcut to get functional male, which proven difficult to be founded from natural catching, is masculinization. This research aimed to induce masculinization of tropical eel, Anguilla bicolor McClelland supplemented with various doses of 17α-methyltestosterone during a month culture in freshwater (0 ppt) or brackish water (10 ppt).  The eel was grouped and fed with supplementation of 17α-methyltestosterone at various doses, depending upon treatments, namely 0 mg Kg-1 diet (control), 40, 80 or 120 mg Kg-1 diet. Eels size were similar, at approximately 16,78 g ± 0,62 in weight and 25,38 cm ± 0,15 in length were either culture in freshwater or brackish during the experiment for eight weeks. Sex gonad, based on anatomical histological structures, Eye Index and Fin Index were measured after time culture treatment achieved, as well as body length, weight, eye diameter and the length of the pectoral fin were measured. Results showed that supplementation 17α-methyltestosterone 80 mg/Kg diet culture in brackish water has the highest number of male (90%).  This study proven that, the hormone was effective for masculinization in eels, It useful for masculinization in eels. Results proved that the 17α-methyltestosterone highly significant (P<0.01) effect on the Eye index (3.63 – 5.14) and Fin Index (3.03 – 4.08) of eels. This study concluded, that 17α-methyltestosterone more effective in improving the number of males in brackish water than in freshwater culture.
Transfer of Green Fluorescent Protein (GFP) Gene to Betta splendens Embryos by Transfection and Electroporation Methods Eni Kusrini; Alimuddin Alimuddin; Erma Primanita Hayuningtyas; Syuhada Restu Danupratama
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.692 KB) | DOI: 10.20884/1.oa.2018.14.2.556

Abstract

Transfection and electroporation method shave a high possibility to apply towards transgenic production of small eggs size fish species.  This study aimed to examine the potential of transfection and electroporation methods to use for transferring a foreign gene into betta fish (Betta splendens) embryos using green fluorescent protein (GFP) gene as a model.  Fish were spawned naturally in the ratio of male: female was 1:1, then a total of 200 eggs were taken for each treatment.  Transfection was performed for 30 minutes (room temperature of about 25 °C) at two-cell stage of embryos using transfast reagent.  Transfection reaction consisted of 0.75 µL transfast reagent, 0.25 µL GFP expression vector (DNA concentration: 50 µg/µL) and 99 µL NaCl solution (concentration: 0,95%).  Electroporation was performed using 125 volt cm-1, 3 times pulse frequency at one second interval and pulse length of 7 micro seconds.  A volume of 800 µL GFP expression vector solution (DNA concentration: 50 µg/ µL) in PBS was used for electroporation.  The successful of foreign gene transfer was determined by PCR method with GFP specific primers.  The results showed that hatching rate of eggs in transfection treatment was 67.08%, while the electroporation was 72.09%.  Survival of larvae in transfection treatment was 73.00%, while the electroporation was 75.00%.  The results of PCR analysis showed that transfection method allowed 65% of the survived fish carrying GFP gene, whereas the electroporation method was 70%.  Thus, foreign gene transfer in betta fish can be conducted using the transfection and electroporation methods. 
Effects of Leads Pb on Gill Microanatomy Structure of Hard-Lipped Barb Fish Osteochilus hasselti Asrul Sahri Siregar; Sofi Alfiyah; Norman Arie Prayogo; Isdy Sulistyo
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.399 KB) | DOI: 10.20884/1.oa.2018.14.2.566

Abstract

As a harmful and non-degradable heavy metal, lead (Pb) is also toxic to aquatic organisms. This metal damages tissue and sensitive organs such as gills. A study dealt with the effect of Pb concentration and contact time on gill microanatomy structure of hard-lipped barb fish (Osteochilus hasselti). An experimental method was applied during study with Completely Randomized Design to test 4 concentrations of Pb, i.e 0 mg/L (P0), 8.93 mg/L (P1), 17.86 mg/L (P2) and 26.80 mg/L (P3), in quadruplicates. The gills were removed, and fixed in Bouin solution, from fish sampled twice in a month. The gills were subsequently processed in classical histology to obtain gill microanatomy structure damage data. The data were established from Pantung method, which were then analyzed with One-way ANOVA. The results showed that Pb damaged gills, mildly to severely, in form of oedema, hyperplatia, lamella fusions, and necrosis. Concentration levels and contact time affected microanatomy structure damage of gills. The increasing concentration levels and length of contact time did not gravely increase in damage.
SOFT CORAL (Sinularia sp.) EXTRACTS WITH ANTIBACTERIAL ACTIVITY Wendy Alexander Tanod; Anita Treisya Aristawati; Masteria Yunovilsa putra; Muliadin Muliadin
Journal Omni-Akuatika Vol 14, No 1 (2018): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (553.859 KB) | DOI: 10.20884/1.oa.2018.14.1.375

Abstract

There is a growing need for new antibacterial agents, in particular because many antibiotics are becoming ineffective due to the emergence of resistant bacterial strains. Soft corals of the Genus Sinularia, Family Alcyoniidae, have potential as a source of terpenoid and steroid compounds with antibacterial activity. These corals may vary in external morphology (shape, colour, size).The aim of this research was to identify extracted fractions with high antibacterial activity. Sinularia sp. specimens were extracted, fractionated based on solvent polarity, and tested for antibacterial bioactivity against pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Antibacterial activity of the three fractions varied in strength. The dichloromethane fraction showed strong antibacterial activity, inhibiting S. aureus and E. coli growth at a concentration of 1 mg ml-1, while the ethyl acetate and ethanol fractions were effective at 10 mg ml-1 and 100 mg ml-1, respectively.