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Meditory : The Journal of Medical Laboratory

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Published by Poltekkes Kemenkes Denpasar

ISSN : 23381159 EISSN : 25491520 DOI : https://doi.org/10.33992/meditory

Core Subject : Science, Social,

Biochemistry, Genetics & Molecular Biology Environmental Science

Meditory : The Journal of Medical Laboratory secara khusus berfokus pada masalah utama dalam pengembangan ilmu di bidang laboratorium medis yang mencakup patologi klinik, mikrobiologi, kimia kesehatan dan patologi anatomi.

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Articles 165 Documents

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PENGARUH VARIASI SUHU PENYIMPANAN TERHADAP VITALITAS SPERMA I Wayan Merta
Meditory : The Journal of Medical Laboratory Vol 5, No 2 (2017): meditory ,Volume 5, No 2, Desember 2017
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Background. Sperm is very sensitive to changes in temperature, this can cause changing the characteristics of spermatozoa. To perform sperm analysis it is necessary to determine the optimal storage temperature to keep the characteristic of sperm samples remain representative.Objective. The purpose of this study to determine the effect of variation in storage temperature on sperm vitality, in order to determine the optimal storage temperature of sperm samples when delay of examination.Methods. In this study we used microscopic method with supravitalstainting using 0,5% eosin staint. Dead spermatozoa will be stained by eosin and live spermatozoa will not be stained by eosin.The study was conducted on 8 subject of the Department of Health Analyst Poltekkes Denpasar that selected by randomly simpling with One Group pretest-posttest design. The measured variable is the value of sperm vitality before and after treatment.Results : Analysis with T-Paired test showed significant difference before and after treatment (p 0,05) before treatment 84,687% ± 3,796, to 78.725% ± 3.809 at 4oC, at temperature 25oC is 82,7875 % ± 3,588, and at 37°C is 70% ± 4,152. Conclusion. From the results of research that has been done, it can be concluded that the decrease in sperm vitality is obtained at least 25oC storage temperature for 1 hour and the most decrease obtained at storage at 37oC for 1 hour. Keywords: Sperm vitality, spermatozoa

TINJAUAN TENTANG KOLESTEROL DARAH PADA SOPIR BUS PARIWISATA DI KABUPATEN BADUNG nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 5, No 1 (2017): Juni 2017 Meditory
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

ABSTRACT Background The lifestyle of consuming high fatty foods, smoking and drinking coffee frequently in the work place could be expected to cause high cholesterol that affects the health of the tourism bus driver. Objective The aims study to determine a cholesterol levels in tourism bus driver at Badung regency. Methods This research is a descriptive study. Tourism bus driver who works at Indah Putra Transport. Result Of the 39 respondents obtained 18% had normal cholesterol levels and 82% had high cholesterol levels. Based on the frequency of smoking every day, the highest frequency indicated by smoking more than 20 cigarettes per day by 25 respondents (64%). The frequency of coffee consumption < 5 cups per day, 41% had high cholesterol levels, consume coffee > 5 cups of coffee per day, 41% have cholesterol levels high. Based on Physical activity habits 3˗4 times a week indicated 5% had high cholesterol levels and physical activity < 3 times a week indicated 77% had high cholesterol levels. 1˗5 year working period earned as much as 8% had high cholesterol levels, 6˗10 year working period earned 3% had normal cholesterol levels and 23% had high cholesterol and the rest of the respondents were 51% in working period above of 11 years have high cholesterol levels. Conclution It can be conceded that bad lifestyle of tourism bus drivers can affect the level of cholesterol. Keywords : driver, cholesterol, lifestyle

KADAR SERUM GLUTAMATE PIRUVAT TRANSAMINASE PECANDU MINUMAN KERAS DI BANJAR AMBENGAN DESA SAYAN UBUD GIANYAR nyoman mastra; I Gusti Ayu Tari Diva Pradnya Dewi; I Wayan Merta
Meditory : The Journal of Medical Laboratory Vol 4, No 2 (2016): Desember 2016
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Background Banjar Ambengan is one banjar contained in Sayan village, where people still have the habit of gathering accompanied by excessive alcohol consumption. Consumption of alcohol has a direct relationship with the death cases caused by liver sirosis. The liver mechanism disturbance leads to the case of swelling as the result of the rise of serum glutamate piruvat transminase (SPGT) enzyme. Objective This study is aimed at investigating the SPGT level of the serum of the alcoholic. The study uses the non-probability sampling technique called snowball sampling. The samples of this study are thirty alcoholic men aging upper eighteen. The respondents will be interviewed to get the deeper information. The research reveals that 6 respondents have high SPGT level (20%) and 24 of them have normal SPGT level (80%). Result of the examination serum SGPT levels alcoholics in the study ranged from 11 to 79.20 U / L. The measurement results SGPT levels are high on the research respondents based on the characteristics of age at most 33.3%, based on the frequency of consumption of at most 33.3%, based on the period of consumption at most 50%, based on the volume of consumption at most 66.7%, based on the type of liquor consumed at most 66.7%, and based on the type of work at most 50%. Conclusion Keywords: Liquor, Liver, Addict, SGPT Levels

KEMAMPUAN DAYA LARVASIDA EKSTRAK DAUN ALPUKAT (Persea americana Mill.) TERHADAP Culex quinquefasciatus Adhi Setya
Meditory : The Journal of Medical Laboratory Vol 6, No 1 (2018): Meditory, volume 6 , nomer 1 , Juni 2018
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Abstract BackgroundCulexquinquefasciatusadalahkelompoknyamuk yang pentingdansalahsatuhama yang seriusdarisudutpandangkesehatanmasyarakat. Pengendalian secara kimiawimerupakanstrategiefektif yang digunakansecaraluassetiapharidikehidupantetapibanyakkonsekuensinegatifdari pemakaiannya. Di antarabiopestisida, tanaman alpukat layak menjadi kandidat penelitian karena kaya akan kandungan fitokimia anti serangga.Method Penelitian ini merupakan uji eksperimental dengan rancangan studi Post-test With Control yang hasilnya diolah menggunakan analisis probit. Konsetrasi dilakukan dengan delapan variasi (0,1%, 0,05%, 0,025%, 0,0125%, 0,006%, 0,003%, 0,001% dan 0,0007%), dengan masing-masing konsentrasi dilakukan tiga kali pengulangan. Sebagai kontrol positif dalam penelitian ini menggunakan temefos. Jumlah larva instar III yang digunakan tiap perlakuan sebanyak 10 ekor. Result Dari hasil fitokimia ekstrak ditemukan alkaloid, flavonoid dan saponin. Analisis probit menunjukan lethal consentration (LC 90) terjadi pada konsentrasi 0,0314% dan kontrol positif berada pada konsentrasi 0,01%. Suhu, kelembaban dan pH menunjukan dalam batas aman untuk larva, yaitu secara berurutan 23oC, 68% dan 6.Conclusion Kemampuan larvasida dari ekstrak daun alpukat berada pada konsentrasi 0,0314%. Hasil ini masih sedikit lebih tinggi dibandingkan kontrol positif (temefos), yaitu 0,01%.Keywords: Culexquinquefasciatus, daun alpukat, ekstrak, larvasida

MAKROFAG DAN ATEROSKLEROSIS IGA Dewi Sarihati
Meditory : The Journal of Medical Laboratory Vol 5, No 1 (2017): Juni 2017 Meditory
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Abstrak Inflamasi dalam aterogenesis terjadi oleh karena adanya interaksi antara leukosit, trombosit dan sel-sel dari dinding pembuluh darah. Rangsangan dari faktor risiko aterosklerosis yaitu hiperkolesterolemi akan meningkatkan produksi monosit di sumsum tulang . Monosit masuk ke arteria dan menempel pada sel endotel serta berubah jadi makrofag setelah sampai di ruang subendotel. LDL yang tinggi pada hiperkolerolemia akan mengakibatkan teradinya oksidasi LDL yang akan difagosit oleh makrofag sehingga berubah menjadi sel busa (foam cell) yang merupakan tahap awal dari aterogenesis. Peningkatan aktivitas dari makrofag M1 akan mendorong terjadinya aterogenesis sementara peningkatan aktivitas makrofag M2 akan mengakibatkan perbaikan dari lesi pada arteri. Untuk mengurangi terjadinya aterosklerosis adalah salah satunya dengan mencegah terjadinya inflamasi pada dindimg pembuluh darah karena hiperkolesterolemia. Kata Kunci: makrofag; aterosklerosis, inflamasi Abstract Inflammation in atherogenesis occurs because of the interaction between leukocytes, platelets and cells of the blood vessel wall. Stimulation of atherosclerosis risk factors as well as hypercholesterolemia will increase the production of monocytes in the bone marrow. Monocytes enter the arteria and attach to the endothelial cells and turn into macrophages after arriving at the subendotel space. High LDL in hypercholerolemia will result in the occurrence of LDL oxidation to be phagocytosed by macrophages and thus transform into foam cells that are the early stages of atherogenesis. Increased activity of M1 macrophages will promote atherogenesis while increased M2 macrophage activity will result in improvement of lesions in the arteries. To reduce the occurrence of atherosclerosis is one of them by preventing the occurrence of inflammation in the walls of blood vesselst due to hypercholesterolaemia. Keywords: macrophages; atherosclerosis, inflammation

GAMBARAN KADAR KALSIUM DARAH PADA WANITA MENOPAUSE DI BANJAR BINOH KAJA, DESA UBUNG KAJA, KECAMATAN DENPASAR UTARA IGA Dewi Sarihati
Meditory : The Journal of Medical Laboratory Vol 4, No 2 (2016): Desember 2016
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

ABSTRACT Background: Menopause women are one of the groups that have a high risk of osteoporosis, because at that time the amount of estrogen is reduced so that the absorption of calcium from the digestive tract will be reduced about 20-25%. This is the reason why the level of calcium decreasing in blood. According to the initial observation at Banjar Binoh Kaja by researcher, from 10 menopause women, four of them are at risk of osteoporosis. Methode: This study is a descriptive study which describes the blood calcium levels in menopause women at Banjar Binoh Kaja, Ubung Kaja Village, North Denpasar District, by doing measurement on their blood calcium concentration with O – Cresolphthalein Complexon (OCPC) method. The 34 respondents taken from total population of menopause women in age range 50-65 years. Respondent was selected using purposive sampling with criteria: menopause women in age range 50-65 years and not undergoing hormone replacement therapy. .Result: The results showed that the average concentration of blood calcium is 8,83 mg/dL which 11.76% respondents had low levels of blood calcium and 88.24% respondents had normal blood calcium levels. 33,33% respondents in age range 62 – 65 years and 66,67% respondents in 16 – 20 years periods of menopause has decrement of blood calcium levels. Coclusion: The decrement of blood calcium levels mostly held in respondents in age range 62 – 65 years and respondents with 16-20 years periods of menopause. Keywords: blood calcium, menopause

GAMBARAN KADAR ASAM URAT, GLUKOSA DARAH DAN TINGKAT PENGETAHUAN LANSIA DI DESA SAMSAM KECAMATAN KERAMBITAN KABUPATEN TABANAN ida arjani
Meditory : The Journal of Medical Laboratory Vol 6, No 1 (2018): Meditory, volume 6 , nomer 1 , Juni 2018
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Background Uric acid is an adjunct product of normal metabolism of the digestion of protein foods containing purines or from the decomposition of purines (damaged body cells), which should be excreted through the kidneys, feces or sweat. While blood glucose levels should be maintained in sufficient concentration to provide nutrients for the organs of the body. Conversely, too high glucose concentrations can also have negative effects such as osmotic diuresis and cell dehydration.The purpose of this study was to determine the description of uric acid levels, blood glucose and level of knowledge of the elderly in Samsam Village, Kerambitan District, Tabanan Regency.Method, The type of research used is descriptive. The population in this study were all elderly in Samsam village area with sample size of 57 samples. Sampling technique used Nonprobability Sampling and sampling with Accidental Sampling.Result.From 57 respondents, there were 14 men (25%), women 43 people (75%), age group ≤ 60 years were 18 persons (32%) and age group 60 years were 39 people (68% ). The results of examination of uric acid levels of respondents in the normal category of 7 people (12%) and high 50 people (88%). Blood Glucose Levels when the respondents were normal 52 people (91%) and high 5 people (9%). Knowledge of respondents was 22 people (39%) in good category and 35 people (61%) in medium category.Conclusion, as many as 88% of respondents have high uric acid levels, 9% of respondents have high blood glucose levels and 61% of respondents have knowledge in the category of being Keywords: Uric Acid Level, Blood Glucose, Elderly’s knowledge

GAMBARAN KADAR KREATININ SERUM PADA PENDERITA DIABETES MELLITUS TIPE 2 DI RUMAH SAKIT UMUM PUSAT SANGLAH DENPASAR ida arjani
Meditory : The Journal of Medical Laboratory Vol 5, No 2 (2017): meditory ,Volume 5, No 2, Desember 2017
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

ABSTRACTBackground Diabetes mellitus (DM) is a metabolic disease which characterized by hyperglycemia due to abnormalities insulin secretions, insulin performance, or both of them. The condition of insulin resistance in DM type 2 causes chronic complications such as diabetic nephropathy. It has become the second leading cause of end-stage kidney disease, and one of the most common and demaging complication of diabetes. The level of creatinine in blood is one of the parameters used to assess renal function, as in the plasma concentration and excretion in the urine within 24 hours. Serum creatinine levels greater than the normal value suggests an impaired renal function. Objective The purpose of this study was to determine serum creatinine levels in patients with DM type 2 in Sanglah General Hospital Denpasar. Methods The method uses an analytical study with description, used accidental sampling methods, involving 30 patients with DM type 2. Blood samples were analyzed for creatinine levels and data are presented as table. The reslts of this study showed that 60% samples had high levels of serum creatinine, 30% samples had normal levels serum creatinine, and 10% samples had low levels serum creatinine. From the result was concluded, most patients with DM type 2 in Sanglah Genaral Hospital have highly serum creatinine levels. Keywords: creatinine serum, DM type 2

GAMBARAN GLUKOSA DARAH DAN KOLESTEROL PADA PEDAGANG DI OBYEK WISATA SANGEH KECAMATAN ABIANSEMAL KABUPATEN BADUNG ida arjani
Meditory : The Journal of Medical Laboratory Vol 5, No 1 (2017): Juni 2017 Meditory
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Bacground The level of glucose in the blood must be kept in a sufficient concentration to provide nutrition for body organs. But on the contrary, the concentration of glucose that is too high can have a negative impact as osmotic diuresis and cellsdehydration. Therefore, blood glucose should be maintained in a constant concentration. Objective The purpose of this study is to describe the levels of blood glucose and cholesterol in traders at Sangeh tourist attraction, Abiansema, District of Badung. Methods This study is a descriptive study. Population of this research was all traders at Sangeh Tourist attraction or about 132 traders. 50 sample was selected by random sampling. Results showed blood glucose levels at any of the 50 samples, 84% normal and 16% higher. Blood glucose levels> 140 mg / dL by age group most in the age range 55 years and over is 12%, the blood glucose levels 140 mg / dL by gender be obtained at most in the female sex is as much as 10%. Test results cholesterol levels of 50 samples of 24% normal and 76% high cholesterol levels> 200 mg / dL based on age in range 55 years and over 36% whereas cholesterol levels 200 mg / dL highs also in the female sex is as much as 60%. Keywords : Blood glucose, cholesterol, traders

ANALISIS KANDUNGAN GIZI EKSTRAK KULIT SALAK PRODUKSI KELOMPOK TANI ABIAN SALAK DESA SIBETAN SEBAGAI UPAYA PENGEMBANGAN POTENSI PRODUK PANGAN LOKAL dhyana putri
Meditory : The Journal of Medical Laboratory Vol 4, No 2 (2016): Desember 2016
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

BackgroundSnakefruit more technically known as ‘Salak’ (Salacca sp) is native fruit of Indonesia regarded as exotic fruit that can consumed not only its flesh fruit but also its skin in the form of extract as it has an efficacy. Extract of snakefruit rind can be proceed into a healthy beverages that contains phenolic compounds which known as natural antioxidants. ObjectiveThis study aimed to describe the content of nutrients such as carbohydrates, proteins, and antioxidants in the extract of snakefruit rind products. This data will be useful as information material to local food industryas well as further research on the processed products for health. Methods This research is a descriptive study that examines the content of carbohydrates, protein, and antioxidants in the extract of snakefruit rind products. Samples were rind of snakefruit with various concentration masses, in water ratio 1%, 5%, 10%, and non compositions. ResultCarbohydrate, protein, antioxidant capacity and IC50 respectively for each composition, are (1) the composition of the 1%: -0.04%; 0.21%; 20.70 ppm, 426.58 ppm; (2) the composition of 5%: 0.82%; 0.20%; 73.40 ppm; 151.41 ppm; (3) the composition of 10%: 0.93%; 0.20%; 110.29 ppm; 87.11 ppm; (4) non composition: 28.14%; 44.90%; 215.10 ppm; 56.10 ppm, and non composition: 1.00%; 0.00%; 25.73 ppm; 92.18 ppm. The most potential nutrient compound in extract of snakefruit rind is the antioxidant, which produced as beverages like tea product. The composition of 10% is the most active category of antioxidant activity, whereas 5% in medium category, and composition of 1% in weak category. Carbohydrates and proteins compound are gradually lower in each composition, caused by boiling process due to the process of water dilution. ConclusionsThis study revealed that extract of snakefruit rind can produced as healthy beverages (such as tea), as it is a potential wastes treatment for local food products. The extract of snakefruit rind consist of nutrition just like proteins, carbohydrates which are great for health and also contains lots of a powerful antioxidant and works well to prevent cardiovascular disease, strokes, and even cancer, prevent allergies, increase fertility, and boost immunity. Keywords: Rind of snakefruit. Antioxidant, Tea

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Home Page

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Contact Name I Gusti Ngurah Dwija Putra

Contact Email dwijaputra07@gmail.com

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Editorial Address Jalan Sanitasi No 1 Denpasar Selatan

Location Kota denpasar, Bali INDONESIA

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Contact Name
I Gusti Ngurah Dwija Putra

Contact Email
dwijaputra07@gmail.com

Phone
+6285772042829

Journal Mail Official
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Editorial Address
Jalan Sanitasi No 1 Denpasar Selatan

Location
Kota denpasar,

Bali

INDONESIA