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Meditory : The Journal of Medical Laboratory
Published by Poltekkes Kemenkes Denpasar
ISSN : 23381159     EISSN : 25491520     DOI : https://doi.org/10.33992/meditory
Core Subject : Science, Social,
Biochemistry, Genetics & Molecular Biology Environmental Science
Meditory : The Journal of Medical Laboratory secara khusus berfokus pada masalah utama dalam pengembangan ilmu di bidang laboratorium medis yang mencakup patologi klinik, mikrobiologi, kimia kesehatan dan patologi anatomi.
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Articles 165 Documents
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GAMBARAN KADAR SERUM INTERLEUKIN-6 PADA PEROKOK AKTIF I Gusti Agung Dewi Sarihati; Kadek Ika Surya Cahyani; Ida Ayu Sri Arjani; Surya Bayu Kurniawan; Heri Setiyo Bekti
Meditory : The Journal of Medical Laboratory Vol 8, No 2 (2020): Meditory, Volume 8, No 2, Tahun 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v8i2.1282

Abstract

AbstractCigarette smoke contains Reactive Oxygen Species (ROS). ROS when it is over-produced causes  oxidative stress. Oxidative stress is related to the synthesis of pro-in-flamatory cytokines  which encourage inflammation which is characterized by the increase in proinflammatory cytokines. The purpose of this study was to determine the description of serum interleukin-6 levels in active smokers. The research sample was 11 active smokers in the Bali Regional Police who were selected using a non- probability sampling technique using purposive sampling. Measurement of serum interleukin-6 levels was performed using the ELISA method. The results showed that serum interleukin-6 levels increased in all age groups and most were found at the age of 35-44 years (36.3%), with normal and obese BMI each 45.5%, in white cigarette smokers with filters (72.7%), moderate smoking intensity (63.6%), with smoking duration of 11-15 years (36.4%). The conclusion in this study is that most active smokers have increased serum interleukin-6 levels. Keywords: active smokers, serum, interleukin-6
ISOLASI CEMARAN ENTEROBAKTER PADA AIR TANAH GAMBUT DI WILAYAH KUBU RAYA Sugito Sugito
Meditory : The Journal of Medical Laboratory Vol 8, No 2 (2020): Meditory, Volume 8, No 2, Tahun 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v8i2.1114

Abstract

Background. Peat soil is a type of soil that is formed from the accumulation of half-decayed plant debris. High organic matter content and some minerals with a thickness of approximately 50 cm. The high content of organic matter in peat soils is thought to be one of the factors that play an important role in supporting the process of adaptation of functional microbes. This is because the availability of organic materials needed by functional microbes for growth and metabolism is met.Method. The sampling method used is purposive sampling. Peat water samples were taken in a composite manner from 2 locations with 10 points on the right and 10 points on the creek flow with a distance of about 10 meters at each point with a trench flow length of 100 meters. Samples were taken in the morning, afternoon, and evening from each point then the inspection was carried out at the Polytechnic Laboratory of the Ministry of Health Pontianak.Results. The distribution of Enterobacter in peat soil water samples taken in the morning, afternoon, the evening is still high with based on identification obtained an average percentage of bacteria Klebsiella Pneumonia 100%, Shigella Dysentery 100%, Proteus Vulgaris 80%, Escherichia Coli 70% and Salmonella 70% and Salmonella Typhi 20%. The coagulation test to determine the type or species of a bacterium from the Enterobacter group is carried out with an antisera test.Concl. There is a relationship between morning water samples with afternoon and evening. Based on the Kendall Tau test, the value of the p-value smaller than α (0.05) is 0.009. 
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN JARAK PAGAR (Jatropha curcas Linn) PADA BERBAGAI KONSENTRASI TERHADAP PERTUMBUHAN BAKTERI Pseudomonas aeruginosa nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 9, No 2 (2021): Volume 9, nomer 2, Desember 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i2.1736

Abstract

Background, Pseudomonas aeruginosa is the main bacterium in nosocomial infections. Pseudomonas aeruginosa is a bacterium that is resistant to almost all of antibiotics. As a result of these resistance cases, it is necessary to find the alternative products derived from natural ingredients antibacterial agents, one of which is Jatropha leaves. Objective, the objective of this study was to know the antibacterial activity of ethanol extract of Jatropha leaves at concentrations of 20, 40, 60 and 80% against the growth of Pseudomonas aeruginosa. Method, This study used experimental posttest only control group design with disc diffusion method used concentrations of 20, 40, 60 and 80%. Positive control using ciprofloxacin antibiotics and negative control using 96% ethanol. Results, of the study obtained the average diameter of inhibitory zones at concentrations of 20, 40, 60%, and 80% respectively were 11.5 mm, 18.3 mm, 19.5 mm, and 20.7 mm. Respectively the analysis of Least Significant Difference test, it was found that there were significant differences in inhibition zones in each extract concentration. Conclusion, the conclusion of this study is that the ethanol extract of Jatropha leaves has antibacterial activity against the bacterium Pseudomonas aeruginosa. Keywords: Jatropha leaves, extract, antibacterial activity, Pseudomonas aeruginosa
Pengaruh Pemberian Streptozotocin Dosis Tunggal terhadap Kadar Glukosa Tikus Wistar (Rattus norvegicus) Nihayah Enis Munjiati
Meditory : The Journal of Medical Laboratory Vol 9, No 1 (2021): Meditory, Volume 9, No 1, Juni Tahun 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i1.1330

Abstract

Diabetes Mellitus (DM) is a disease caused by metabolic disorders in the pancreas, which is characterized by an increased glucose levels or hyperglycemia due to decreased insulin levels from the pancreas. In animal research, DM is often caused by administration of diabetogenic agents. Streptozotocin works by forming free radicals that can damage the pancreatic beta cells; therefore, insulin production can be disrupted. This study aims to determine the glucose level on Wistar Rats (Rattus norvegicus) before and after a single dose streptozotocin-induced. This study was conducted experimentally with a posttest-interpretation design with a control group design. The result demonstrated that the average blood glucose level before the induction of streptozotocin was 92,63 (SD 10,487) mg/dL, after being induced by streptozotocin was 235,56 (SD 164,929) mg/dL with a difference of 142,938 (SD 160,543) mg/dL. In this study, it can be concluded that the induction of a single dose of streptozotocin has an effect on increasing blood glucose levels on Wistar Rats (Rattus norvegicus).
GAMBARAN ANGKA LEMPENG TOTAL DAN IDENTIFIKASI BAKTERI Escherichia coli PADA LOLOH BUNGA TELENG DI DESA PENGLIPURAN KECAMATAN BANGLI nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 9, No 1 (2021): Meditory, Volume 9, No 1, Juni Tahun 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i1.1307

Abstract

AbstractBackground : Teleng flower herb is a processed beverage made from teleng flower which is popular as a traditional drink from Penglipuran Village. As one of the beverage products, teleng flower herbs have to obtain consent from the BPOM before distributed and consumed. Purpose : this study was conducted to analyze the quality of microbiology of teleng flower herb. The analyzed parameters were total plate count (ALT) and E. coli bacteria identification. Method : this study used descriptive research with laboratory examinations which was conducted at the Bacteriology Laboratory. The 7 samples were obtained from producers with three times repetition so that it became 21 samples with sampling techniques were saturated sampling. Result : The ALT examination results show that 5 samples (71.4%) are not qualify and 2 samples (28.6%) are qualify based on BPOM Regulation Number 16 In 2016. The obtained results of E. coli identification show that there were 4 (57.1%) contain of E. coli pathogens and 3 samples (42.9%) are not contains of E. coli pathogens. It can be concluded that most of the hygiene of teleng flower herb at Penglipuran Village does not meet the requirements. Conclusion : Based on these results, we suggest to the producers of teleng flower herb to maintain the quality of teleng flower herb that they sell. Key words: teleng flower herb, microbiological quality, total plate count, E. coli
UJI EFEKTIVITAS EKSTRAK BUAH BALONGGA (Cucumis melo L. forma agrestis) SEBAGAI INSEKTISIDA ALAMI TERHADAP NYAMUK Aedes sp. Sri Aprilianti Idris
Meditory : The Journal of Medical Laboratory Vol 9, No 2 (2021): Volume 9, nomer 2, Desember 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i2.1441

Abstract

Background: Aedes sp is a vector of Dengue Hemorrhagic Fever (DHF). DHF is a disease caused by the dengue virus of the genus Flavivirus, family Flaviviridae. DHF is transmitted from person to person through the bite of the Aedes aegypti and Aedes albopictus (female) mosquitoes. Balongga fruit is a wild plant typical of Southeast Sulawesi which is usually consumed by the people of Southeast Sulawesi as a food ingredient such as vegetables and to add flavor to fish dishes. Balongga fruit contains secondary metabolites, namely flavonoids, saponins and tannins which have the ability to inhibit the growth of larvae and mosquitoes.Objective: The purpose of this study was to determine the results of the effectiveness test of the extract of Balongga fruit (Cucumis melo L. forma agrestis) as a natural insecticide against Aedes sp.Methods: This research was conducted experimentally, in the test using four treatments with variations in concentration, namely 20%, 40%. 60% and 80%, negative control (aquadest) and positive control X-Electric (transflutrin 12.38 g/l)), and the number of mosquitoes used was 25 in each treatment with three repetitions. The observation time required in this study is 1440 minutes. Observations were also made by counting the dead mosquitoes in each treatment with time intervals of 5, 10, 20, 40, 60, 120, 240, 480, and 1440 minutes. The data in this study were analyzed using the one-way ANOVA test.Conclusion: The results showed that the 80% concentration of Balonga fruit flesh extract was the most effective as a natural insecticide to kill Aedes sp mosquitoes with an interval of 240 minutes.
Gambaran kolesterol total pada lansia di Puskesmas I Denpasar Selatan I Gusti Agung Ayu Putu Swastini
Meditory : The Journal of Medical Laboratory Vol 9, No 2 (2021): Volume 9, nomer 2, Desember 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i2.1526

Abstract

background Total Cholesterol is the amount of cholesterol carried In all cholesterol carrying particle In the blood including HDL, LDL, and VLDL. THR elderly will experience the aging process and decreased physiology function change which can lead tO reduced activity of the receptors of controlling the amount of choledterol In the body. The normal value of total cholesterol level is normal 200 mg/dl, high limit 200-239 mg/dl, and high 240 mg/dl. Risk factors can affect total cholesterol level are age gender, BMI, and blood pressure. The purpose of this study was tO determine to description of total cholesterol levels In elderly. The research method uses discritive research with purpisive sampling method involving 115 respondents take using secondary data for the elderly at Puskesmas I South Denpasar. THR results showed That most elderly respondents who experienced an increase total cholesterol were female as many as 26 people (22,6%) the age group 65-74 years as many as 18 people (15,7%), In the normal BMI category as many as 26 people (22,6%), and normal bolos pressure as many as 25 people (21,7%), high limit total cholesterol levels as many 45 people(31,9%), and high total cholesterol levels as many as 45 people (31,9%).
MEDIA ALTERNATIF PERTUMBUHAN Staphylococcus aureus DARI BIJI DURIAN (Durio Zibethinus murr) Siti Juariah
Meditory : The Journal of Medical Laboratory Vol 9, No 1 (2021): Meditory, Volume 9, No 1, Juni Tahun 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i1.1400

Abstract

Media that is often used for bacterial growth is Nutrient agar. The high price incurred for the manufacture of instant media becomes a constraint so that innovation is needed that can reduce the cost of expenditure that is looking for alternative media from materials that are easily available. One of the natural ingredients that have a source of nutrients that can be used for bacterial growth of durian seeds that have a high carbohydrate content as a source of carbon. The objective of this research was to know the growth of Staphylococcus aureus bacteria on alternative medium of durian seed as substitution media of Nutrient agar. This research is laboratory experimental research, the sample used is durian seed with three concentrations of 200 g, 300 g, 400 g. The parameters observed were calculating total plate number and morphological form of Staphylococcus aureus bacteria growth. The results showed that the highest population on medium durian medium was the concentration of 200 g as much as 5.7 x 10-4 cfu and the lowest population at the concentration of 400 g was 3.5 x 10-3 cfu. While the population on the media Nutrient agar that is 7.7 x 10-6 cfu. Based on morphological observation of bacterial growth on durian seed medium, round shape, size 0.1 cm, white milk color, smooth surface, and convex elevation. The conclusion of this research is the growth of Staphylococcus aureus on alternative medium of durian seeds can be used as substitution media of Nutrient agar.
DETEKSI KEBERADAAN TELUR Soil Transmitted Helminth (STH) PADA KUKU PETANI DITA PRATIWI KUSUMA WARDANI
Meditory : The Journal of Medical Laboratory Vol 9, No 2 (2021): Volume 9, nomer 2, Desember 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i2.1698

Abstract

Soil-Transmitted Helminth (STH) is an intestinal nematode that requires soil for the process of reproduction and maturation from non-infective to infective. The nematodes included in this species include Ascaris lumbricoides, Trichuris trichiura, and two species of hookworm, Necator americanus, and Ancylostoma duodenale. Personal hygiene can affect the incidence of helminth infections in humans. The purpose of this study was to determine the relationship between personal hygiene and the presence of Soil-Transmitted Helminth (STH) eggs on farmers' nails. This research was conducted by analytical observation with a cross-sectional design in April-May 2021. The research method was filling out questionnaires and identifying eggs using the 10% KOH sedimentation method. Data were analyzed by univariate and Pearson Chi-Square tests. The results of this study showed that 4 fingernail samples (47.05%) were positive for Ascaris lumbricoides eggs and 2 fingernail samples (11.76%) were positive for Minute Intestinal Fluke, as many as 8 toenail samples (82.35%) were positive for Ascaris lumbricoides eggs. and 1 sample of toenails (5.88%) positive for Minute Intestinal Fluke eggs. There was no relationship between personal hygiene and the presence of STH eggs on farmers' nails (p = 0.05). STH eggs were found in the fingernails and toenails of farmers in Kaligondang District, Purbalingga Regency. Farmers need to improve personal hygiene to avoid helminth infections.
GAMBARAN ANGKA LEMPENG TOTAL DAN IDENTIFIKASI Escherichia coli PADA BAKSO AYAM YANG DIJUAL DI DESA SANUR KAUH DENPASAR SELATAN I Gede Sudarmanto
Meditory : The Journal of Medical Laboratory Vol 9, No 2 (2021): Volume 9, nomer 2, Desember 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i2.1746

Abstract

ABSTRACTIntroduction: Chicken meatballs are a typical Indonesian processed meat food made from a mixture of chicken meat, tapioca flour, and spices. Chicken meatballs are one of the favorite foods of the society, but several studies on meatballs still show the result that meatballs unqualified in health.Aims: The aim of this study was to determine the quality of bacteriology of chicken meatball which are sold in Sanur Kauh Village. The parameters analyze were total plate count and identification of Escherichia coli bacteria. Methods:This research used description study with laboratory examination conducted at Panureksa Utama Laboratory. Total sample that taken in this study were 12 samples of chicken meatball. The sampling technique is using saturated sampling. Results: Based on total plate count examination, it was found from 12 samples chicken meetballs that 3 samples (25%) unqualified and 9 samples (75%) qualified according to SNI No. 01-3818:2014 about Bakso Daging. The results of identification of Escherichia coli bacteria was found that 1 sample chicken meatball (8,3%) unqualified and 11 samples (91.7%) qualified according to Permenkes No. 1096/MENKES/PER/VI/2011.Discusion: Based on the results, it can be conclude was the most of chicken meatballs was qualified in health. The sellers must be attentive about sanitation and personal hygiene during processing of chicken meatballs.Keywords: chicken meatballs, quality of bacteriology, total plate count, Escherichia coli.

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