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Meditory : The Journal of Medical Laboratory
Published by Poltekkes Kemenkes Denpasar
ISSN : 23381159     EISSN : 25491520     DOI : https://doi.org/10.33992/meditory
Core Subject : Science, Social,
Biochemistry, Genetics & Molecular Biology Environmental Science
Meditory : The Journal of Medical Laboratory secara khusus berfokus pada masalah utama dalam pengembangan ilmu di bidang laboratorium medis yang mencakup patologi klinik, mikrobiologi, kimia kesehatan dan patologi anatomi.
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Articles 165 Documents
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HASIL PEMERIKSAAN IMUNOKROMATOGRAFI COACTAIL ANTIGEN M.TUBERCULOSIS DAN METODE MOLEKULER PADA SUSPEK HIV Rizana Fajrunni'mah
Meditory : The Journal of Medical Laboratory Vol 8, No 1 (2020): Meditory, vol. 8 no 1 Juni 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.66 KB) | DOI: 10.33992/m.v8i1.1093

Abstract

Background Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) infection. Tuberculosis is one of the most common infections in people with HIV/ AIDS. Deaths due to TB in HIV patients occur due to delayed diagnosis. GeneXpert is a breakthrough discovery for TB diagnosis based on molecular examination using the semi quantitative Real Time Polymerase Chain Reaction Assay (RT-PCR) method. An examination has now been developed to detect MTB antigens using the method of rapid immunochromatography in the hope that it can be used as a means of supporting TB diagnosis better than detection of antibodies to MTB.Aim This study aims to compare the results of the TB GeneXpert RTPCR method with the rapid immunochromatography method in suspect HIV.Method This study uses a quantitative design with observational analytic design (cross sectional) with primary and secondary data collection.Result The results of this study found the value of sensitivity, specificity, accuracy, positive predictive value, negative predictive value of the ICT test in a row of 21%, 76%, 40%, 55.8%.Conclusion ICT TB has low sensitivity and sufficient specificity so that it is still not good if used for initial screening to detect pulmonary TB in suspect HIV. 
UJI FITOKIMIA DAN KAPASITAS ANTIOKSIDAN TEH RING CANG SEBAGAI TEH HERBAL KESEHATAN i wayan karta
Meditory : The Journal of Medical Laboratory Vol 8, No 1 (2020): Meditory, vol. 8 no 1 Juni 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.791 KB) | DOI: 10.33992/m.v8i1.1107

Abstract

Background: Diets that contain lots of antioxidants will reduce the risk caused by degenerative diseases. Based on this, many people develop food or health products that contain antioxidants. Jempiring leaves (Gardenia jasminoides Ellis) and Secang wood (Caesalpinia sappan L.) contain bioactive and antioxidant compositions that are beneficial to health, so they are developed into herbal products.Purpose: To analyze phytochemical reserves, antioxidant capacity and conduct organoleptic tests for Ring Cang tea, describing the benefits of ring rings based on laboratory test results and literature studies on jempiring leaves and secang wood.Method: This research is a descriptive study by conducting phytochemical testing laboratory tests and DPPH antioxidant methods and conducting organoleptic tests with 15 panelists.Results: Ring tea can contain flavonoids, tannins, terpenoids, alkaloids and polyphenols. Ring Cang tea has an antioxidant capacity of 291.43 mg / L and IC50 1.53 μg / mL which shows the antioxidant activity of ring cang tea in the very strong category. Ring tea can be tested organoleptically accepted by panelists in terms of color, taste, and aroma, so that it can be developed into herbal teas for the community.Conclusion: Cang tea is an antioxidant tea that can be used in handling generative or degenerative diseases, such as diabetes, antiviral, anti-inflammatory, and other diseases.
PENGARUH SUHU DAN WAKTU PEMANASAN TERHADAP KETAHANAN TELUR Ascaris lumbricoides Ni Putu Aryadnyani
Meditory : The Journal of Medical Laboratory Vol 8, No 1 (2020): Meditory, vol. 8 no 1 Juni 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v8i1.1113

Abstract

The prevalence of intestinal nematode infection in Soil Transmitted Helminth (STH) is still high in Indonesia because the tropical climate and high rainfall greatly affect soil moisture as an important medium in its life cycle. One of the STH species is Ascaris lumbricoides which is transmitted through food contamination, especially raw vegetables. Eggs from Ascaris worms have resistance to strong acids, strong bases, oxidants, reductants, and surface-active agents. Ascaris lumbricoides eggs are resistant in 10% formalin dissolved in aquadest, NaCl 0.85%, and sodium phosphate. Ascaris lumbricoides eggs also have durability in 10% formalin solution which is heated at 60°C, 70°C and 80°C. This study aims to determine the resistance of Ascaris lumbricoides eggs to temperature and heating time. This research is expected to provide benefits in proving the temperature and heating time can affect the resistance of Ascaris lumbricoides eggs, so that it can provide certainty and a sense of security for the public that the heating process in food, especially vegetables can cause Ascaris lumbricoides eggs to be inactive.This research is a true experimental study with the design of The Randomized Posttest Control Group Design. The treatments used are heating temperatures 80°C, 90°C and 100°C for 10 minutes, 20 minutes, and 30 minutes. Based on the research, it was found that Ascaris lumbricoides eggs could not withstand heating 80°C, 90°C and 100°C for 10 minutes, 20 minutes, and 30 minutes. Suggestions in this study are to avoid Ascaris lumbricoides infection by increasing personal hygiene and always consuming foods, especially vegetables that have been heated for at least 80 ° C for 10 minutes.
Gambaran Methicillin Resistant Staphylococcus aureus (MRSA) Pada Petugas Kesehatan RSUD Wangaya Kota Denpasar Ida Bagus Oka Suyasa
Meditory : The Journal of Medical Laboratory Vol 8, No 1 (2020): Meditory, vol. 8 no 1 Juni 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.681 KB) | DOI: 10.33992/m.v8i1.1074

Abstract

Background.        Methicillin Resistant Staphylococcus aureus (MRSA) is a Staphylococcus aureus bacterium that is immune to penicillin isoxazoyl antibiotics such as methicillin, oxacillin and flucloxacillin. Staphylococcus aureus is one of the normal flora of the anterior nares and skin, which can be pathogenic when the patient's immune condition is deteriorating.Purpose. to determine the percentage of Methycillin Resistant Staphylococcus aureus (MRSA) in health staff at Wangaya District Hospital in Denpasar.Method. type of research is descriptive research design with Cross Sectional. The results of nasal swabs were implanted on MSA media and then catalase and coagulase tests were performed. The methicillin resistance test is carried out if after a positive catalase test and a positive coagulase test. If found MRSA will be continued by sensitivity test with antibiotics based on CLSI.Results.  found five isolates of Staphylococcus aureus (11.36%), one MRSA isolate (2.27%), sensitive antibiotics were Tetracyclin, Cephazolin, Trimethroprim, Amikacin, Ciprofloxacin, Doxycycline, Ofloxacine and Levofloxacine.Conclusion. One MRSA isolate (2.27%) founded from nasal swabs of health staff at Wangaya District Hospital in Denpasar.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BUAH KAWISTA (LIMONIA ACIDISSIMA) TERHADAP ESCHERICHIA COLI Putu Rika rika Veryanti; Ika Maruya Kusuma
Meditory : The Journal of Medical Laboratory Vol 8, No 2 (2020): Meditory, Volume 8, No 2, Tahun 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v8i2.1269

Abstract

Escherichia coli is a normal bacteria lives in the large intestine to help the process and maintain human digestive health. However, there are certain types of Escherichia coli which are pathogenic to human body and cause infections, such as diarrhea, urinary tract infections, meningitis, sepsis and so on. Irrational use of antibiotics leads to bacterial resistance to antibiotics. Kawista is one of the fruits that used as traditional medicine in Indonesia. Kawista peel extract can be used as an alternative therapy to prevent resistance. Based on the content of secondary metabolites, kawista peel extract is thought to have antibacterial activity. The purpose of this study was to determine the antibacterial activity of kawista (Limonia accidisima) peel extract against Escherichia coli. This research is an in-vitro study, preceded by a phytochemical screening test for methanol extract of kawista peel, then followed by an antibacterial activity test using the disc diffusion method and determining the minimum inhibitory concentration (MIC). The results showed that the kawista peel extract contained secondary metabolites such as alkaloids, flavonoids, saponins, tannins and terpenoids which have antibacterial activity. The concentration of the extract is directly proportional to its antibacterial activity. The 5% extract concentration gave an average inhibition zone diameter of 8.81 mm (moderate antibacterial activity), while at the 80% extract concentration the average inhibition zone diameter was 22.71 mm (very strong bacterial activity). Kawista peel extract (Limonia acidissima) has antibacterial activity with a minimum inhibitory concentration of 5%. Keywords: Antibacterial, Kawista, Limonia acidissima, Escherichia coli
FORMULASI DAN UJI AKTIVITAS ANTI BAKTERI GEL PEMBERSIH TANGAN EKSTRAK ETANOL MIDING (STENOCHLAENA PALUSTRIS) Ratih Indrawati
Meditory : The Journal of Medical Laboratory Vol 8, No 2 (2020): Meditory, Volume 8, No 2, Tahun 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v8i2.1122

Abstract

Background Kalimantan is the largest island in Indonesia which has a tropical climate with abundant biological resources. Biodiversity has the potential to be used as medicinal plants. Plant extracts are preferred as antibacterial, one of which is miding plant (Stenochlaena palustris).Aim This study aims to find out the potential of miding plant extracts as an antibacterial against Staphylococcus aureus.Method Miding was extracted using maceration using 70% ethanol solvent. Ethanol miding extraction showed a yield of 3.12% (w / w).Result The extract obtained was carried out by phytochemical test, phytochemical test of miding ethanol extract showed the composition of alkaloid, flavonoid, stereoid, phenolic, and saponin groups.Conclusion Antibacterial activity test results using the well diffusion method. Formula 30%, 20 and 10%. Obtained inhibition zone diameter of 12.50 mm and 20% of 12.00 mm showed greater antibacterial activity compared to a comparison of only 10.02%.
EFEKTIFITAS EKSTRAK BONGGOL NANAS (Ananas comosus L. Merr) TERHADAP Escherichia coli Siti Juariah; Diana Wati
Meditory : The Journal of Medical Laboratory Vol 8, No 2 (2020): Meditory, Volume 8, No 2, Tahun 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v8i2.1246

Abstract

Escherichia coli is one of the bacteria that lives in digestive system and could cause a diarrheal. Diarrheal treatment is done by using antibiotic.  The use of antibiotic in continuous way could be affected the bacteria resistances toward antibiotic. However, the study on natural anti-bacterial substance is being carried out using pineapple hump. This study was aimed to determine the secondary metabolites that contain in pineapple hump and the inhibition test of Escherichia coli by using experimental laboratory method in vitro. Pineapple hump extract created in many concentrations are 10%, 15%, 20%, 25%, 40%, 60%, and 80%, after that inhibition test of Escherichia coli was done and it will be incubated during 1x24 hours. The result  Shows that pineapple hump extract gain percentage of an inhibition test of Escherichia coli in concentrations 40%, 60%, and 80% are 50%, 63%, and 75%, compared with positive control and active compounds that contain in pineapple hump are bromelin, flavonoid, tannin, and saponin enzymes. This result could be concluded that pineapple extract hump potentially inhibit Escherichia coli which is marked by the formation of inhibitory zone.
TINJAUAN KEAMANAN PANGAN, HYGIENE SANITASI DAN KANDUNGAN GIZI MAKANAN TRADISIONAL DI KABUPATEN TABANAN Ni Putu Widyartini; I Gst Putu Sudita Puryana; Anak Agung Nanak Antarini
Meditory : The Journal of Medical Laboratory Vol 8, No 2 (2020): Meditory, Volume 8, No 2, Tahun 2020
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v8i2.1136

Abstract

Traditional food is food made in the traditional way using traditional materials and equipment. Traditional foods consist of complete meals, side dishes, vegetables and traditional snacks that are in high demand by local and national tourists so that their safety needs to be tested. The purpose of this study is to determine the Food Safety, Sanitation Hygiene and Nutrition Content of Traditional Traditional Foods Tabanan Regency. This type of research is observational with cross sectional study design. Traditional Foods of Tabanan Regency there are 103 food samples and the sample size used is 30 samples (19%) of the total sample. Food safety contained 15 (50%) exceeded the maximum limit for coliform contamination and 1 (3%) sample exceeded the limit of Escherichia Coli contamination. Hygiene Traditional food sanitation all food safety scores from traditional food processors are included in the category of vulnerable but safe for consumption. Nutritional content of traditional foods in complete foods contains high energy, namely 321,9g (14.3%) of adequacy, in side dishes containing high protein 11,6g (19.2%) of adequacy and fat that is 20,8g (32%) of adequacy, in vegetables containing high fat which is 8g (12,3%) of adequacy and in snacks contain high carbohydrate which is 67,2g (18.7%) of adequacy.Keywords: Traditional Food, Food Safety, Sanitation Hygiene, Nutrition
NILAI INDEKS TROMBOSIT SEBAGAI KONTROL KUALITAS KOMPONEN KONSENTRAT TROMBOSIT Dewi Astuti
Meditory : The Journal of Medical Laboratory Vol 8, No 2 (2020): Meditory, Volume 8, No 2, Tahun 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v8i2.1238

Abstract

Platelet component therapy is given to patients who have thrombocytopenia and platelet dysfunction, therefore an assessment of the quality of platelet components is necessary. The platelet component evaluation in UTDD PMI DKI Jakarta was carried out on the parameters of blood type test, volume, platelet count, pH and macroscopic examination of platelets (swirling) once on days 1, 2 or 3. This study aims to determine the value of the platelet index during the shelf life of the platelet concentrate component as a quality control. The research method uses a cross sectional experimental approach. Platelet index analysis was performed using Sysmex SN 350 hematology analyzer on five platelet component samples. The quality of platelet components is assessed based on the presence or absence of the difference between the platelet index values during the shelf life. Statistical results show that there are significant differences in the values of MPV, PDW and PCT from the shelf life of the 2nd day until the 5th day, but the examination results are still within normal limits. Platelet index results show an increasing trend. This indicates an increase in platelet volume and variations in platelet form. The conclusion of this study, the platelet component has good quality until the 5th day, and the platelet index value can be used as a platelet quality control parameter.
PERBANDINGAN IDENTIFIKASI Toxoplasma gondii MENGGUNAKAN METODE POLYMERASE CHAIN REACTION (PCR) DAN METODE ENZYME LINKED FLUORESCENT ASSAY (ELFA) Miftahul Mushlih; Alifia Nurfitriana; Kurnia Wahyu Ningsih; Nurul Azizah; Nia Lukita Arian
Meditory : The Journal of Medical Laboratory Vol 8, No 2 (2020): Meditory, Volume 8, No 2, Tahun 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v8i2.1266

Abstract

Toxoplasmosis is an infectious disease that does not show specific symptoms. This study aimed to determine the differences results of T. gondii detection in human blood using the PCR (polymerase chain reaction) with the ELFA (Enzym Linked Fluorescent Assay) method. The research was done by descriptive exploratory methods. Samples taken from 9 West Sumatra Pramita Clinics Laboratory and 1 person came from Sidoarjo as a control. Data analyzes was conducted by a chi-square test with a 95% confidence level. In the ELFA method detects IgG Anti-Toxoplasma. Whereas the molecular test detects  B1 gene target. The serological test was able to detect 9 patients of toxoplasmosis and 1 patient was negative/ control. The molecular test showed a specific band with 195 bp in length. The accuracy of the molecular test was 77% (T count (4.4) T table (3.84). Based on the results its can be concluded that there a difference in identification between the PCR method and the ELFA method.

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