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INDONESIA
JOURNAL OF COASTAL DEVELOPMENT
Published by Universitas Diponegoro
ISSN : 14105217     EISSN : -     DOI : -
Core Subject : Education,
Education
The Journal of Coastal Development (ISSN 1410-5217) is dedicated to all aspects of the increasingly important fields of coastal and marine development, including but not limited to biological, chemical, cultural, economic, social, medical, and physical development. The journal is jointly published by the Research Institute of Diponegoro University, Semarang, Indonesia and Indonesian Assosiation of Oceanologist (ISOI).
Arjuna Subject : -
Articles 328 Documents
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OPTIMIZATION OF Bacillus sp. K29-14 CHITINASE PRODUCTION USING MARINE CRUSTACEAN WASTE Agustinus Robert Uria; Ekowati Chasanah; Yusro Nuri Fawzya
JOURNAL OF COASTAL DEVELOPMENT Vol 8, No 3 (2005): Volume 8, Number 3, Year 2005
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Chitin is present in large quantities in the marine crustacean waste disposed by seafood processing industries, making it very desirable as the substrate for producing chitinase, a hydrolytic enzyme of considerable interest in many industrial and agricultural applications. In our work, crustacean waste powder and its combination with colloidal chitin at different concentrations (0.5, 1.0, and 1.5%) were utilized to optimize the chitinase production by the bacterium, Bacillus sp. K29-14. The results showed that the chitinase production with the three different substrate concentrations was relatively constant in the range of 0.2 to 0.3 U/ml during 12 days cultivation, although there was a bit reduction after day 8. This activity profile seems to be similar to that of the protein content. Whereas the chitinase production on the media containing crustacean waste powder and its combination with colloidal chitin at the three concentrations showed that the highest activity (3.0 to 4.6 U/ml) was achieved on day 7 and 8. The specific chitinase activity with the waste powder at different concentrations of substrate (0.5, 1.0 and 1.5%) was increasing slowly during a nine-day cultivation. The optimal chitinase production (4.6 U/ml) was achieved with the combined substrate of 0.5% on day 8.
GROWTH INHIBITION OF MEDICALLY ANTIBIOTIC RESISTANT BACTERIA BY SPONGE-ASSOCIATED BACTERIA Ocky Karna Radjasa
JOURNAL OF COASTAL DEVELOPMENT Vol 11, No 2 (2008): Volume 11, Number 2, Year 2008
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Abstract

The improper and uncontrolled uses of antibiotics against pathogenic bacteria have resulted in theoccurrence of Multi Drugs Resistant bacteria. There is now an urgency to find alternative antibioticsto combat these bacteria. The metabolites from microorganisms are a rapidly growing field, due, atleast in part, to the suspicion that a number of metabolites obtained from algae and invertebrates maybe produced by associated microorganisms. Therefore, there is a shift in the search for secondarymetabolites from terrestrial to marine environment. Sponge-associated microorganisms are among ofthe most interesting and promising marine natural product sources, which produce polyketide and nonribosomal peptide products with various biological activities. In this study, marine bacteria wereisolated from sponge Haliclona sp. collected from North Java Sea, and were screened for antibacterialactivity against MDR strains. One out of 32 bacterial isolates were successfully screened and werefound to be active against MDR strains, strain Escherichia coli and strain Proteus sp., respectively.These active isolates were also capable of amplifying NRPS gene fragments necessary for thebiosynthesis of non ribosomal peptides. The identification results revealed that the active isolates areArthrobacter sp.
APPLICATION OF REPETITIVE SEQUENCE-BASED PCR ON THE RICHNESS OF VIBRIO ON THE TIGER SHRIMP (Penaeus monodon Fab.) S arjito; Nestin E.W. Ningrum; Ocky Karna Radjasa; Slamet Budi Prayitno
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
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Abstract

A molecular-based study was conducted to estimate the richness of the Vibrio on  tiger shrimp (Penaeus monodon Fab.) from brackish water pond of Mororejo, Kendal. Tiger shrimps were collected from the extensive brackish water pond and a total of 22 isolates were obtained from hepatopancreas and telson of tiger shrimp. Based on the repetitive sequence-based polymerase chain reaction (rep-PCR), it was found that two groups of Vibrio. To investige the efectiveness of rep-PCR in estimating the richness of Vibrio on tiger shrimps, three isolates  (JTW 01, JTW 03 and JTW 06) were chosen for further investigation. On the basis of sequence analysis, the result showed  that the JTW 01, JTW 03 and JTW 06 were closely related to Vibrio sp. Absa7 clone 423.1, Vibrio splendidus and Vibrio splendidus, respectively.  The result proved that two assosiated of Vibrio on tiger shrimp were Vibrio sp. Absa7 clone 423.1 and Vibrio splendidus. Therefore  the present study highlights the effectiveness of rep-PCR in rapid grouping and estimating the richness of Vibrio on  tiger shrimp.
OSMOTIC RESPONSE OF TIGER SHRIMP (Penaeus monodon) BROOD STOCK IN VARIOUS MOLTING STAGES Sutrisno Anggoro; Agung Setiarto
JOURNAL OF COASTAL DEVELOPMENT Vol 2, No 1 (1998): Volume 2, Number 1, Year 1998
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Abstract

The attempt to optimize shrimp culture production has faced with several problems related to its biotechnical management, which was suspected unable to meet the needs of the cultured species. This is primary because the ecophysiological characteristics of shrimp, particularly those related to the osmoregulation mechanism in connection with their growth, have not been sufficiently understood. The research was aimed at studying the ecophysiological characteristics of tiger shrimp (Penaeus monodon) involved in biotechnical management of shrimp hatchery with special emphasis on the osmotic response and isosmotic medium requirement for adult spawners in various molting stages. The results showed that osmotic responses were closely realted to the salinity of water medium and molting phases. It was also found that the minimum osmotic works of tiger shrimp occurred in isosmotic medium, i.e. 34 – 35 ppt for premolt, 33 – 34 ppt for molt and 30 – 31 ppt for intermolt stages.
CHARACTERIZATION OF CAROTENOID PIGMENTS FROM BACTERIAL SYMBIONTS OF SEAGRASS Thalassia hemprichii Sri Achadi Nugraheni; Miftahuddin M Khoeri; Lia Kusmita; Yustin Widyastuti; Ocky Karna Radjasa
JOURNAL OF COASTAL DEVELOPMENT Vol 14, No 1 (2010): Volume 14, Number 1, Year 2010
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Abstract

Carotenoids are pigments that can be used in various applications including cosmetics and precursor ofvitamins A. Carotenoids are mostly found in higher plant leaves, fruit, and bacteria. Marine bacteriaassociated with seagrass Thalassia hemprichii collected from Menjangan Kecil Waters, KarimunjawaIslands were screened to produce the pigment and has allowed the use of these microrganism as anenvironmental friendly alternative source of new natural pigment. The isolation of bacterial symbionts onZobell 2216E medium from seagrass Thalassia hemprichii resulted in 20 isolates of which 8 bacterialsymbionts have produced pigments but only one bacterium positively synthesize carotenoids. Initial analysiswith atomic absorption spectrophotometric method revealed that the wave lengt of bacterial pigment were inthe range of 300-600 nm, which are categorized that within the group of carotenoid pigments. From theresults of molecular identification by 16S rDNA method, it was shown that bacterium TH8 was closelyrelated to Bacillus licheniformis with 98% homology value.
COASTAL AND MARINE ENVIRONMENT PROBLEMS IN EAST KALIMANTAN PROVINCE Supriharyono .
JOURNAL OF COASTAL DEVELOPMENT Vol 3, No 2 (2000): Volume 3 Number 2 Year 2000
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Abstract

Coastal and marine problems were collected from the coastal community in area of the Municipality of Balikpapan East Kalimantan Province, from fishermen, NGO’s and government officials. The technique used was that of the Field Participatory Workshop’s. the result of the study revealed some coastal community awarenees, coastal resources usage, and governmental policy. The local government’s failure to solve those problems was mainly due to lack of social awarenees for environmental coastal management and lack enforcement.
ANTIBACTERIAL ACTIVITY OF SPONGE ASSOCIATED-BACTERIA ISOLATED FROM NORTH JAVA SEA Ocky Karna Radjasa
JOURNAL OF COASTAL DEVELOPMENT Vol 10, No 3 (2007): Volume 10, Number 3, Year 2007
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Abstract

A total of 90 bacterial isolates were obtained from association with sponges collected from Bandengan water, Jepara and Karimun island of Karimunjawa islands in the north java sea. Antibacterial screening by using a pathogenic Escherichia coli indicated that two isolates BSP.12 and MKSP.5 inhihited the growth of tested strain. Molecular identification based on 16S rDNA approach revealed that isolate BSP.12 was closely related to Vibrio harveyi with a homology of 100% and isolate MKSP.5 showed highest similarity to Brachybacterium rhamnosum (99%), respectively.A PCR-based approach by using specific primers targeting the occurrence of gene fragments necessary for the biosynthesis of major natural products, namely Non-ribosomal peptide synthetases (NRPS) and Polyketide synthases (PKS) was applied to estimate the genetic potential of these active strains. Both isolates were capable of amplifying the NRPS gene fragments but not the PKS gene fragments.
INTERACTIONS OF SQUID AND SMALL PELAGIC RESOURCES IN THE ALAS STRAIT, INDONESIA A. Ghofar
JOURNAL OF COASTAL DEVELOPMENT Vol 6, No 1 (2002): Volume 6, Number 1, Year 2002
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Abstract

Small pelagic and squid fisheries of the Alas Strait in Indonesia are compared, to explore potential interactions  between major components caught, which may then be taken into management consideration. The data time series used for analysis were taken from major fishing harbours and landing places, and go back as far as 1970s. The small pelagic fishery in Alas Strait is mainly targeted upon squid (mostly Loligo edulis) by means of ‘jala-oras’ (payang-type) fishing, which is also used to catch small pelagic fishes during squid-off season. The small pelagic catches consist primarily of lemuru (Sardinella lemuru),  tembang (Sardinella fimbriata), layang scad (Decapterus spp), and kembung (Rastrelliger spp), which are usually taken by various pelagic fishing gears. There was a general increase in the small pelagic catches from around 4,000t in 1976 to nearly 12,000t in 1990, after which a considerable fluctuation occurred, reaching a level of 8,000t in 1999. Squid catches  increased steadily from <100t to 1,700t prior to1978 due to “jala-oras” boat motorization, but fluctuated sharply afterward. Another peak catch may be observed to approach 1,900t in 1997. During this period of large fluctuation, again, the landings  of small pelagic fishes tend to replace squid position. There is a strong indication of the existence of interaction between squid and small pelagic fisheries. In general the overall small pelagic exhibits a strong linear relationships  with a correlation coefficient, r, of 0.5270 (highly significant). More specifically their species components also shows even stronger correlations, with coefficient correlation of 0.5898, 0.6686 and 0.6358 respectively for layang, kembung and lemuru. Interactions of the species group and their  substantial implication in fisheries research and management are discussed in this paper
MAPPING OF CORAL REEF ECOSYSTEM IN THE NATUNA ISLANDS USING ALOS IMAGERY Suyarso .; Yaya Ihya Ulumuddin; Bayu Prayuda
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 1 (2011): Volume 15, Number 1, Year 2011
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Abstract

The coral reef ecology of the Natuna Islands has been studied over 10 years. However, none of those studies produced a coral reef map. Maps of coral reef ecosystem are important for planning, management and monitoring tool. The present study integrates the 115 field data and the ALOS satellite data, using depth invariant index algorithm to generate coral reef ecosystem classes. Those classes are: life corals, dead coral and rubble aggregates, mixing of substrates and sand. The algorithm that composed of three visible bands is applicable at clear water rather than at turbid water environment. Hence, vegetation coverage as well as seagrass, seaweed and macro algae which are in small extent and usually covered by fine sand materials and associated with turbid water, cannot be classified.The aim of this research, which is funded by Critic Coremap – LIPI, is to produce map of coral reef ecosystem in the Natuna Islands.
DISTRIBUTION AND SPECIATION OF HEAVY METALS (Cd, Cu AND Ni) IN COASTAL SEDIMENTS OF DUMAI SUMATERA, INDONESIA Bintal Amin; Ahmad Ismail; Aziz Arshad; M Salleh Kamarudin
JOURNAL OF COASTAL DEVELOPMENT Vol 10, No 2 (2007): Volume 10, Number 2, Year 2007
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Abstract

Heavy metal concentrations in sediment collected from Dumai coastal waters have been studied by using sequential extraction technique to determine the distribution and geochemical speciation (EFLE, acid reducible, oxidisable organic and resistant) of Cd, Cu and Ni. The results showed that the highest concentrations of total Cd was in Cargo Port area and the lowest in Penyembal, whilst for Cu and Ni the highest concentrations were in Ferry Port and the lowest in Batu Panjang.  The total concentrations of Cd, Cu and Ni ranged from 0.65 – 1.82, 1.84 – 13.16 and 7.68 – 17.98 µg/g dry weight, respectively. Higher metal concentrations were detected in the eastern and central parts of Dumai city center where most of anthropogenic activities are concentrated. However, most of the concentrations of Cd, Cu and Ni were still below the ERL and ERM values. Only at few stations, especially in the eastern and central parts of Dumai, showed Cd concentrations exceed the ERL but still well below the ERM values. Metal concentrations in 78.26% (Cd) and 91.30% (Cu and Ni) of the sampling stations were dominated by resistant fraction indicating natural origin of these metals. Non resistant fractions, however, were found higher than resistant fractions at Pelintung, Pertamina, Cargo Port, Penyembal and Batu Panjang for Cd; Penyembal and Batu Panjang for Cu and Pelintung and Guntung for Ni. These findings indicated that anthropogenic inputs of Cd, Cu and Ni occurred in these stations. For all sampling site groups, Cd, Cu and Ni were mostly accumulated in the resistant fraction (55.28 – 58.31%; 65.02 – 91.84% and 50.08 – 66.88% of the total concentrations respectively) which indicated that the mobility and anthropogenic inputs of these metals in Dumai coastal waters were quite low.

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