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ILMU KELAUTAN: Indonesian Journal of Marine Sciences

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Published by Universitas Diponegoro

ISSN : 08537291 EISSN : 24067598 DOI : -

Core Subject : Science,

Earth & Planetary Sciences

ILMU KELAUTAN: Indonesian Journal of Marine Sciences (IJMS) is dedicated to published highest quality of research papers and review on all aspects of marine biology, marine conservation, marine culture, marine geology and oceanography.

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Articles 743 Documents

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Molecular Determination of a Gren Algae Isolate to Detecting 1-Deoxy-D-Xylulose-5-phosphate Synthase (DXS) Gene in Improvement of Carotenoid Production Hermin Pancasakti Kusumaningrum; Joedono Soedarsono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 11, No 2 (2006): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Sintesis karotenoid alami belum pernah melebihi produk sintetik pada skala komersial. Kurangnya pemahaman mengenai aspek mikrobiologis dan ekofisiologis isolat penghasil karotenoid menyebabkan terjadinya kesalahan penamaan spesies. Satu isolat lokal alga hijau dari BBAP Jepara yang digunakan sebagai pakan alami sumber karotenoid hewan-hewan perikanan, pada mulanya dianggap sebagai Dunaliella. Namun pengembangan produksi karotenoid menggunakan teknologi rekayasa genetik dan rekayasa metabolit terhadap isolat algahijau lebih lanjut memperlihatkan ketidaksesuaian hasil dengan penamaan yang ada. Akumulasi karotenoid jalur non-MVA pada alga hijau ditentukan oleh enzim D-1-Deoksixilulosa 5-fosfat Sintase, yang disandi oleh gen D-1-deoksixilulosa 5-fosfat sintase (DXS). Determinasi spesies secara molekuler menjadi penting dilakukan untuk menentukan spesies isolat dan jalur biosintesis karotenoid yang digunakan. Hasil determinasi digunakan untuk analisis keserupaan putative partial fragment gen DXS Isolat alga hijau yang telah berhasil diperolehpada penelitian sebelumnya. Tujuan utama penelitian ini adalah menentukan spesies satu isolat lokal alga hijau secara molekuler menggunakan 23S rRNA untuk mendeteksi keberadaan gen DXS penyandi biosintesis karotenoid. Hasil penelitian memperlihatkan bahwa Isolat alga hijau menunjukkan keserupaan yang tinggi dengan anggota-anggota Sianobakteria. Keserupaan tertinggi dimiliki dengan Cyanobacterium sp. MBIC 1021 sebesar 99 %, diikuti Synechocystis PCC6308 sebesar 95 %. Satu-satunya anggota Cyanobacteria yangmemiliki gen DXS adalah Synechocystis. Hasil analisis keserupaan parsial gen DXS isolat alga hijau terhadap tujuh parsial gen DXS pada daerah lestari yang telah ditemukan, memperlihatkan bahwa putative partialfragment gen DXS Isolat lokal alga hijau juga memiliki keserupaan tertinggi dengan gen DXS Sianobakteria Synechocystis.Kata kunci: isolasi alga hijau, Dunaliella, gen DXS, 23S rRNA, Cyanobacteria, SynechocystisCarotenoids production levels are not yet competitive with carotenoid levels presently produced by fermentation, synthesis and isolation. It needs application of metabolic engineering and genetic engineeringtechniques in improving their production. An attempt to optimize carotenoid production from local isolate of green algae from BBAP Jepara has faced several problems, primarily related to the microbiological and ecophysiological characteristic which affecting growth that have not sufficiently been understood. A misnamed of species also have arisen due to wrong characterization. One local isolate of an algal species from BBAPJepara was found potentially useful as source of carotenoids in food additives or as food supplement in fish farming. It was suspected as representing a strain of Dunaliella. Previous studies to improve carotenoidproduction using molecular approach on have shown unagreement. Therefore, the present study aimed to determinate the species of green algae isolate from Jepara waters based on molecular techniques using 23S rRNA approach for detecting DXS gene. Molecular analysis by 23S rRNA alignment showed the close relationship among isolate of green algae and most all of member of Cyanobacteria. Closest similarities wasshowed by Cyanobacterium sp. MBIC 1021 with 99 % similarity and Synechocystis PCC6308 with 95 % similarity. Synechocystis was the only member of Cyanobacteria which have DXS gene. Multiples aligmentsequences of partial DXS gene on the conserve region among seven species confirmed this result. The DXS gene analysis also showed closest relationship between partial DXS gene of Cyanobacteria Synechocystis anda green algae isolate. The result of this analysis proven as valuable parameter for the interpretation of the relation among DXS gene of a green algae isolate and Cyanobacteria and increase the possibility in getting the complete DXS gene from local isolate of green algae by designing primers from DXS gene of Synechocystis as a member of Cyanobcteria.Key words: a green algae isolate, Dunaliella, DXS gene, 23S rRNA, Cyanobacteria, Synechocystis

Asexual Reproduction of Black Sea Cucumber from Jepara Waters Retno Hartati; Muhammad Zainuri; Ambariyanto Ambariyanto; Sri Redjeki; Ita Riniatsih; Ria Azizah; Hadi Endrawati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 24, No 3 (2019): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Black sea cucumber or Lollyfish are trade name for Holothuria atra, one species of family Holothuriidae abundance in Jepara waters, especially in Panjang Island. They inhabit on the seabed, in shallow waters on reefs and sand flats or in Seagrass meadows. Beside reproduce sexually, H. atra also do fission (biology), i.e. able to reproduce asexually by transverse fission. Monthly survey has been conducted for three months to determine frequency of fission among H. atra population in Panjang Island waters. In total 891 individu of H. atra inhabit in seagrass meadow mixed with rubble were examimed. In fissiparous sea cucumber, transverse fission are followed by regeneration and in this research identified from external observations. The fission state was divided into three category, i.e. closed wound (Category 1), little regeneration either posterior or anterior part (category 2), moderate regeneration either posterior or anterior part (category 3). The result showed that the fission frequency was highest during end of rainy season in April (13,21%) and decreased during May (4,61%) and June (4,86%). Body regeneration seem happened fast, since the new individu sea cucumber at category 1 was low but high as category 3. The regeneration were related with the condition of environment.

Pengaruh Beda Porositas Terumbu Buatan Tipe Silinder Berongga (Bottle Reef TM) Sebagai Submerged Breakwater Terhadap Kinerja Peredaman Gelombang (Effect of Porosity Differences of Hollow Cylinder (Bottle Reef TM) Types of Artificial Reefs as Submerged ..) Rudhy Akhwady; Mukhtasor Mukhtasor; Haryo D Armono; Mahmud Musta’in
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 17, No 2 (2012): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Gesekan antar luas permukaan terumbu buatan dengan gelombang dapat menyebabkan hambatan aliran, sehingga menyebabkan turunnya energi gelombang. Untuk itu perlunya direncanakan kesesuaian antara luas permukaan dengan model bentuk terumbu, sehingga diperoleh tingkat reduksi dan stabilitas agar struktur dapat bekerja dengan optimal. Hal ini dengan mempertimbangkan kinerja aliran yang terjadi denganmemberikan porositas aliran agar dapat mengalir melalui celah-celahnya sehingga gesekan antara gelobang dengan struktur terumbu buatan tidak akan berlangsung secara ekstrim dan terus menerus, dikarenakanmembahayakan struktur tersebut. Hasil pengujian di laboratorium wave flume dengan menggunakan gelombang tipe irreguler menunjukkan bahwa, untuk memperbesar peredaman gelombang dilakukan dengan memperkecil nilai koefisien transmisi, yaitu dengan cara memperbesar kecuraman gelombang, memperbesar lebar puncak relatif dan memperkecil freeboard. Dengan semakin kecil diameter rongga dan jumlah rongga yang sedikit, kemampuan reduksi juga makin meningkat di bandingkan dengan rongga yang berjumlah sama tetapi berdiameter lebih besar. Dari hasil penelitian ini, diharapkan nantinya dapat menjadi sumber informasi tentang kinerja terumbu buatan model bottle, dan dapat gunakan sebagai salah satu alternatif struktur habitat buatandan peredam gelombang yang dapat bekerja secara optimal dengan memperhatikan dan aspek lingkungan.Kata kunci: bottle reef, submerged breakwater, porositas, transmisi Interaction among reef surface with ocean wave, causing current barrier that might reduce wave energy. Therefore it was necessary to plan a compatibility of surface and shape in order to gain a degree of reductionand stability so that structure could work optimally. This consider the occurrences of current performance by giving porosity for current to be able to flow through its gap so that the friction between wave and structurewould not take place in an extreme way continuously that would jeopardize the structure. The result test of wave flume in laboratory with irreguler wave showed that to increase wave damping it was done by enlarging and reducing the peak relative width of freeboard. By having smaller porous diameter and smaller amount of porous, the ability of reduction will also increase compare to same amount of porous but had bigger porous diameter. Finally, this result could be as an information in project of bottle reef, and could also be used as one of marine habitat structure and wave damping alternative that worked optimally and had an environmental friendly view. Key words: bottle reef, submerged breakwater, porosity, transmission

Isolasi dan Karakterisasi Bakteri Hidrokarbonoklastik dari Perairan Dumai dengan Sekuen 16S rDNA Nursyirwani Nursyirwani; Kathy Copper Amolle
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 12, No 1 (2007): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Penelitian ini dilaksanakan dari bulan September sampai Desember 2006. Tujuan penelitian adalah untuk mempelajari karakteristik molekuler bakteri hidrokarbonuklastik yang diisolasi dari perairan laut Dumai berdasarkan sekuen 16S rDNA. Isolasi bakteri dilakukan pada media cair dan padayang ditambahk an Sumatran crude oil. Karakteristik molekuler diperoleh melalui isolasi dan ampHdkasi DNA dengan PCR dan sekuensing menggunakan ABI 3130 Genetic Analyzer. Dari 6 isolat yang danalisis, hanya ada 3 isolatyang dapat disekuensing. Dari perbandingan dengan BLAST database, didapatkan kesamaan sekuen yang terdekat untuk isolat CA (91%) adalah Providenda vermicola, isolat DA (93%) adalah Burkholderia cepacia, dan isolat FA (99%) adalah Myroides odoratimimus.Kata kunci: Bakteri hidrokarbonuklastik, PCR dan 16S rDNAThe research was conducted from September to December 2006. The aim was to study molecular characterization of hidrocarbonoclastic bacteria based on sequence 16S rDNA from Dumai waters. The bacteria was isolated in both broth and solid media added with the Sumatran crude oil. Molecular characterization included DNA isolation and amplification using PCR, and sequencing by ABI 3130 Genetic Analyzer. Three of six isolateswere successfully sequenced. The comparison of 16S rDNA with known 16S rDNA sequences from BLAST database showed that the closest sequence similarity of isolate CA (91%) was Providencia vermicola, isolate DA(93%) was Burkholderia cepacia, and isolate FA (99%) was Myroides odoratimimus.Key words : Characterization, Hydrocarbonoclastic Bacteria, PCR, 16S rDNA

Assessment of Carbon Status in Marine Protected Area of Payung Island Waters, South Sumatera Province, Indonesia Anna Ida Sunaryo Purwiyanto; Fitri Agustriani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 1 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

CO2 is a greenhouse gas that receive more attention than the other gases because the properties of carbon easily deformed and diffuseed. Changes in the concentration of CO2 in the water will impact on changes in the amount of CO2 in the atmosphere that affect sea surface temperatures. It continuously will result in a change of marine capture fisheries. Payung Island is one of the important areas in South Sumatra that acts as the provider of the fishery. This because Payung Island is located in the mouth of Musi and Telang River covered by mangrove, has a very important ecological function. However, the condition of the carbon in the waters of the Payung Island has not explored further. This elementary study is to determine status on Payung Island waters as a sink or source of CO2. The study was conducted in June until August 2015. The research stages include surface water sampling, measurement of the CO2 in the atmosphere, the analysis of the concentration of Dissolved Inorganic Carbon (DIC) and Total Alkalinity (TA), and partial pressure of carbon dioxide (pCO2) calculation. Atmospheric CO2 were measured insitu, while the DIC and TA were analyzed using titration methods. Partial pressure of carbon dioxide (pCO2) obtained from the calculation using the software CO2Calc using data of DIC, TA, nutrients and atmospheric CO2. The results showed that the content of DIC and TA on the Payung Island waters has similar distribution pattern i.e. high in areas close to the river, and getting lower in the area which were closer to the sea. The comparisons between pCO2 atmosphere and pCO2 waters showed that Payung Island waters generally act as a carbon sink in area towards the sea but however, in the territorial waters adjacent to the river as a source of carbon. Keywords: carbon, marine protected area, Payung Island waters

Studi Perubahan Densitas Zooxanthellae pada Translokasi dan Transplantasi Karang Acropora aspera dan Stylophora pistillata di Jepara Rohani Juniarta; Errien N Aisyah; Munasik Munasik
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 10, No 4 (2005): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Karang hidup bersimbiosis dengan zooxanthellae, dimana karang mendapat zat-zat makanan dan O2 sebagai hasil fotosintesis dan sebaliknya zooxanthellae mendapat perlindungan, zat hara dan CO2. Hubungan simbiosis mutualisme ini dipengaruhi faktor biotik dan abiotik. Jika salah satu faktor tersebut melewati batas normal dapat mengakibatkan karang memutih atau “bleaching” dan untuk memulihkannya kembali memerlukan waktu yang lama. Salah satu cara yang dilakukan adalah dengan memakai teknik translokasi dan transplantasi. Untukpelaksanaan teknik ini, dipilih karang Acropora aspera dan Stylophora pistillata dan dilakukan pengamatan terhadap densitas zooxanthellae dari ke dua karang tersebut. Karang dipindahkan dari perairan P. Panjang dan ditanam di perairan Teluk Awur Jepara. Pada akhir waktu penelitian, A. aspera bisa bertahan hidup sedang S. pistillata mengalami kematian. Pengamatan terhadap densitas zooxanthellae, yang meliputi zooxanthellae sehat, berproliferasi dan terdegradasi selama penelitian, menunjukkan adanya fluktuasi tergantungdari kondisi karang.Kata kunci: zooxanthellae, translokasi, transplantasi, A. aspera, S. pistillata.Life corals perform symbioses with zooxanthellae, where corals get nutritions and O2 as photosynthesize results of zooxanthellae, whereas zooxanthellae get shelter, trace elements and CO2. This mutual symbioticrelation is affected by biotic and abiotic factors. If one of these factors is beyond the normal limit, it can cause the corals exposed to bleaching, and it will take a long time for the corals to be recovered. One of themethods to manage this is by using translocation and transplantation techniques. For this purpose, Acropora aspera and Stylophora pistillata were used and zooxanthellae density of both corals was analysed. The coralswere moved from Panjang Island waters and transplanted in Teluk Awur waters. At the end of the analyses, A. aspera could survive but S. pistillata was bleaching. Analyses on the zooxanthellae densities, consisting ofthe healthy, proliferated and degradated zooxanthellae, show fluctuations depending on corals condition.Key words: zooxanthellae, translocation, transplantation, A. aspera, S. pistillata.

Dampak Aktivitas Perkotaan dan Penambangan Nikel Terhadap Tingkat Kontaminasi Logam Berat dalam Air Laut dan Sedimen (Impact of City Activities and Nickel Mining Toward Heavy Metal Contaminant in the Seawater and Sediment) Fasmi Ahmad
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 18, No 2 (2013): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Teluk Kendari dan Teluk Lasolo yang terletak di Sulawesi Tenggara banyak menerima limbah berasal dari beberapa sungai yang bermuara ke teluk tersebut dan dari kegiatan penambangan logam nikel di daratan dan kawasan pantai. Limbah tersebut mengandung berbagai macam bahan kontaminan, termasuk logam berat. Tujuan penelitian adalah untuk mengetahui tingkat kontaminasi logam berat dalam air laut dan sedimen di perairan Teluk Kendari dan Teluk Lasolo dalam kaitannya untuk kepentingan biota laut. Pengukuran kadar logam berat Pb, Cd, Cu, Zn dan Ni dalam air laut dan sedimen di Teluk Kendari dan Teluk Lasolo. Contoh air laut diambil dengan menggunakan Rosette Water Sampler dan sedimen dengan gravity core pada 10 stasiun penelitian. Kadar logam berat diukur dengan menggunakan Spektrofotometer Serapan Atom (SSA). Hasil penelitian menunjukkan kadar logam berat Pb, Cd, Cu, Zn dan Ni dalam air laut dan sedimen di Teluk Lasolo lebih tinggi dibandingkan dengan Teluk Kendari. Tingginya kadar ke lima logam berat di Teluk Lasolo ini disebabkan oleh limbah yang berasal dari aktivitas penambangan nikel di kawasan darat dan pantai Lasolo. Hasil penelitian ini menunjukkan bahwa kadar ke lima logam berat di kedua perairan teluk ini masih aman untuk biota laut. Kata kunci: sedimen, air laut, logam berat, pencemaran Lasolo, Kendari Kendari and Lasolo Bay which were situated in Southeast of Sulawesi. Both of Bay have been received a lot of waste originated from a variety of activities in Kendari City and Nickel mining activities in the land and in coastal area of Lasolo region. Waste contains a variety of materials contamination, one of these contaminants are heavy metals. The aim of this research is to determine the level of heavy metal contamination in seawater and sediment in Kendari and Lasolo Bay in their relation to the interests of marine organisms. Measurement of levels of heavy metals Pb, Cd, Cu, Zn and Ni in sediment in the Kendari and Lasolo Bay has been done in June 2011. Seawater and sediment samples have taken by using a Rosette water sampler and gravity cores at 10 research stations. Heavy metal levels were measured by Atomic Absorption Spectrophotometer (AAS). The results showed that the concentration of heavy metals Pb, Cd, Cu, Zn and Ni in seawater and sediments in Lasolo Bay is higher compared to Kendari Bay. This condition is believed to be attributable from the nickel mining activities in the land and coastal area of Lasolo. Generally, the concentration of those heavy metals in both of that bay still saved for marine organism Keywords: sediment, seawater, heavy metal, pollution Lasolo, Kendari

Assessing the Organic Pollution and Aquaculture Activity of Surface water at Shatt-Al-Arab Estuary Southern of Iraq Makia M. Al-Hejuje; Prof. Hamid Talib Al-Saad; Najah A. Hussain
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 4 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Surface water samples were collected monthly from December, 2012 to November, 2013 at the middle part of Shatt Al-Arab Estuary at Basrah city during the low tide period to assess the organic pollution status using the Organic Pollution Index (OPI), and the aquaculture activities using Minimum Operator Concept (MOC). Results of biological oxygen demand (BOD5), ammonium, nitrite and orthophosphate were used to calculate OPI, while dissolved oxygen (DO), total phosphate and turbidity were used to calculate MOC. Results showed that OPI values range from 4.5 - 4.75 referred to weak organic–non organic pollution status. On the other hand, the water of Shatt Al-Arab Estuary can be considered as medium–excellent water for aquaculture activity according to the MOC range (66.67 - 93.33). Keywords: Estuary, Surface water, Organic Pollution Index, Aquaculture activity, Minimum Operator Concept

Potensi Berbagai Jenis Lamun Sebagai Sumber Makanan Kesehatan : Analisis Proksimat Wilis A Setyati; Subagiyo Su8bagiyo; Ali Ridlo
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 10, No 2 (2005): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.10.2.115-118

Penelitian ini bertujuan untuk mempelajari status nutrisi berbagai jenis lamun yang terdapat diperairanBandengan, Jepara berdasarkan analisis proksimat (gula reduksi, serat kasar, lemak, protein dan abu). Penelitian ini juha melakukan analisis vegetasi lamun di perairan Bandengan, Jepara. Hasil penelitian menunjukan ada 6 jenis lamun yang tumbuh di perairan Bandengan Jepara yaitu Cymodecea serrulata, Thalassia hemprichii, Enhalus acoroides, Syrirgodium isoetifolium, Halodule uninervis dan Thalassodendron ciliatum. Keenam jenis lamun tersebut berturut-turut mempunyai kepadatan 109,00 ind/m2, 106,87 ind/m2, 89,35 ind/m2 , 82,97ind/m2 , 73,65 ind/m2 dan 18,75 ind/m2. Hasil analisis proksimat utuk ke enam jenis lamun tersebut berturut turut adalah sebagai berikut, Cymodecea serrulata mempunyai kandungan protein, gula reduksi, lemak, abu dan serat kasar berturut-turut (% berat kering) 9,39 ; 0,91; 7,81; 67,09; 19,25, Thalassia hemprichii berturut turut 8,35; 1,10 ; 7,38; 62,43; dan 17,27, Enhalus acoroides berturut-turut 7,65 ; 1,00; 6,13 ; 68,14 ; dan 19,92, Syrngodium isoetifolium berturut-turut 5,52 ; 2,19; 4,71; 70,62 dan 12,16. Sedangkan Halodule uninervis dan Thalassodendron ciliatum tidak dilakukan analisis proksimat karena jumlah vegetasi yang adasangat sedikit.Kata kunci : Lamun, analisis proksimat, analisis vegetasiThe research was done to answer how about nutritive profile (protein, lipids, reduction sugar ash and crudefibre) of some seagrass and how about some seagrasses can be improved potentially as foodhealthness. In this research vegetaion analyze or seagrasses also was done, to identified rheir diversity and density. The research found 6 species of seagrass in seagrass filed of Bandengan waters, Jepara, i.e. Cymodecea serrulata, Thalassia hemprichii, Enhalus acoroides, Syrirgodium isoetifolium, Halodule uninervis dan Thalassodendron ciliatum. There have population density respectively 109,00 ind/m2, 106,87 ind/m2, 89,35 ind/m2 , 82,97 ind/m2, 73,65 ind/m2 and 18,75 ind/m2.The result of proximate analyze showed that Cymodecea serrulata haveprotein , sugar reduction, lipid, ash and crude fibre (% dry weight) respectively 9,39 ; 0,91; 7,81; 67,09;19,25, Thalassia hemprichii 8,35; 1,10 ; 7,38; 62,43; dan 17,27, Enhalus acoroides 7,65; 1,00; 6,13; 68,14 ; and 19,92, Syrngodium isoetifolium 5,52 ; 2,19; 4,71; 70,62 and 12,16. Halodule uninervis and Thalassodendron ciliatum have not beeb abalyzed.Key words: seagrass, proximate analysis, vegetation analysis.

Akumulasi dan Depurasi Toksin PSP (Paralytic Shellfish Poisoning) oleh Kerang Hijau (Accumulation and Depuration of PSP Toxin (Paralytic Shellfish Poisoning) by Green Mussels) Haryoto Kusnoputranto; Setyo S Moersidik; Djarot S Wisnubroto; Murdahayu Makmur
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 19, No 1 (2014): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Ledakan mikroalga sering dilaporkan terjadi di Teluk Jakarta, dimana di lokasi tersebut juga terdapat kegiatan budidaya kerang hijau (Perna viridis). Terkait dengan hal tersebut maka dilakukan studi akumulasi dan depurasi toksin PSP (Paralytic Shellfish Poisoning) pada kerang hijau. Studi akumulasi dilakukan di bagan kerang hijau perairan Cilincing Jakarta Utara, dengan memisahkan kerang hijau yang berukuran sama dan ditempatkan kembali ke bagan. Sampling dilakukan setiap minggu selama 2 bulan dan diukur juga kelimpahan fitoplankton, pH, suhu dan salinitas perairan. Depurasi dilakukan di Unit Depurasi Kekerangan KKP Panimbang Banten, yang dilakukan selama 24 jam. Pencuplikan sampel dilakukan setiap jam pada 4 jam pertama dan setiap 2 dan 3 jam pada waktu berikutnya. Penentuan konsentrasi toksin PSP dilakukan dengan menggunakan HPLC detektor fluoresensi. Prosedur preparasi, ekstraksi dan pengukuran konsentrasi toksin mengikuti Manual AOAC Official Method 2005.06 untuk toksin PSP dalam kekerangan. Akumulasi toksin PSP oleh kerang hijau di perairan Cilincing pada bulan Januari–Pebruari 2011 berkisar antara 4,11–11,96 µg STX eq. per 100 g dan tidak mempunyai korelasi dengan kelimpahan Dinoflagelata di perairan. Hal ini disebabkan uji akumulasi tidak dilakukan pada saat blooming mikroalga. Uji depurasi selama 24 jam mengeliminasi toksin PSP sebesar 60%, sehingga bisa diajukan sebagai sistem pemutus rantai toksin dari mikroalga ke manusia. Kata kunci: akumulasi, depurasi, PSP toksin, kerang hijau, Cilincing Microalgae blooms have been frequently reported in the Jakarta Bay, which is also the location of green mussel (Perna viridis) aquaculture. Accumulation and depuration of Paralytic Shellfish Poisoning (PSP) toxin in the green mussels were investigated in the field, where the toxin accumulation studies conducted in the mussel farming at Cilincing, North Jakarta. Accumulation test carried out by placing back the selected green mussel (equal size) into the mussel farming. Every week for 2 months, the green mussel were collected from mussel farming and transported to the laboratory. The fitoplankton abundance also was checked including pH, Suhue and salinitiy paramaters. Toxin depuration was conducted at Clams Sanitation Unit at Panimbang Banten. The depuration studies were conducted for 24 hours with sampling every hour in the first 4 hours and every 3 and 2 hours until the 24th hour. Preparation, extraction and toxin concentration measurements performed by following the Manual AOAC Official Method 2005.06 for PSP toxin in oyster. This research concluded that the accumulation of PSP toxin by green mussel, Perna viridis in the mussel farming at Cilincing, North Jakarta in ranged between 4,11–11,96 µg STX eq. per 100 g during January–February 2011. No correlation between PSP toxin concentration in the green mussel, Perna viridis with abundance of the PSP toxin sources phytoplankton, because the study wasnt done when microalgae blooming. The depuration processes was eliminate 60% the PSP toxins for 24 hours depuration processing. It can be proposes as a banded system the PSP toxin from algae to human being. Keywords: accumulation, depuration, PSP toxin, green mussel, Cilincing

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