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ILMU KELAUTAN: Indonesian Journal of Marine Sciences

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Published by Universitas Diponegoro

ISSN : 08537291 EISSN : 24067598 DOI : -

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Earth & Planetary Sciences

ILMU KELAUTAN: Indonesian Journal of Marine Sciences (IJMS) is dedicated to published highest quality of research papers and review on all aspects of marine biology, marine conservation, marine culture, marine geology and oceanography.

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Aktivitas Antioksidan dan Toksisitas Ekstrak Metanol dan Pigmen Kasar Spirulina sp. Ervia Yudiati; Sri Sejati; Sunarsih Sunarsih; Rani Agustian
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 4 (2011): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Penelitian ini bertujuan untuk mengukur aktivitas antioksidan dan menguji toksisitas ketiga ekstrak. Penelitian dilakukan secara laboratoris. Ekstrak Kasar Spirulina sp didapatkan melalui proses maserasi dengan pelarut methanol. Ekstrak pigmen kasar diperoleh melalui partisi dengan pelarut methanol/aseton serta eter. Aktivitas antioksidan dilakukan dengan mengukur IC50 dengan metode spektrofotometri dengan DPPH (1,1-diphenyl-2-picrylhydrazyl) sedangkan kadar toksisitas dilakukan dengan menghitung LC50 dengan uji BSLT (Brine Shrimp Lethality Test). Kromatografi Kolom Terbuka dilakukan untuk mengisolasi pigmen sedangkan identifikasi pigmen dilakukan dengan teknik Kromatografi Lapis Tipis dan metode spektroskopi. Data dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa ekstrak kasar metanol, ekstrak pigmen kasar methanol/aseton dan eter mempunyai nilai IC50 323,7; 51 dan 34,85 ppm. Nilai LC50 dari ekstrak methanol r, ekstrak kasar pigmen methanol/aseton dan eter berturut-turut adalah 113,20; 65,22 ppm dan 34,11 ppm. Hasil isolasi pigmen dan identifikasi pigmen menunjukkan bahwa pigmen mengandung β-karoten dan klorofil α. Hasil uji fitokimia menunjukkan bahwa ketiga ekstrak positif mengandung senyawa golongan flavonoid dan sterol. Ekstrak pigmen kasar Spirulina sp. selain mempunyai aktivitas antioksidan yang tinggi juga mempunyai toksisitas yang tinggi terhadap nauplii Artemia sp. Kata kunci: Spirulina sp,ekstrak metanol, ekstrak kasar pigmen, IC50, LC50. The aims of this research were to determine the antioxidant activities and level of toxicity of those three extracts. Spirulina sp crude extract has obtained by maseration technique with methanol solution while crude pigment extract has collected by partition technique with methanol/acetone and ether solution. The laboratory experiments of antioxidant activities (IC50) was determine by DPPH (1,1-diphenyl-2-picrylhydrazyl) and spectrophotometrical methods while the level of toxicity was done by BSLT (Brine Shrimp Lethality Test). The pigment was isolated using the Couloumb Chromatography Methods while pigment identification was done by Thin Layer Chromatography Methods and confirmed spectrophotometrically. The data was analized descriptively. The results showed that IC50 of methanol extract, crude pigment methanol/acetone and ether were 323.7; 51 and 34.85 ppm, respectively. Furthermore, the toxicity level LC50 of methanol extract, crude pigment methanol/acetone and ether were 113.20; 65.22 and 34.11 ppm respectively. β-carotene and chlorophyll α was identified and isolated. Those three extract contains flavonoid and sterol. It is concluded that crude pigment extract of Spirulina sp. has a high antioxidant activity as well as toxicity to the nauplii of Artemia sp. Key words: Spirulina sp.,methanol extract, crude pigment extract, IC50, LC50

Pestisida Organoklorin pada Aqifer Dangkal di Wilayah Pesisir Kota Semarang Chrisna Adhi Suryono; Baskoro Rochaddi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 14, No 3 (2009): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Studi ini bertujuan untuk menganalisis kondisi fisika-kimiawi dan tingkat kontaminasi pestisida organoklorin dalam aqifer dangkal di wilayah pesisir Kota Semarang. Sampel airtanah diambil di 10 lokasi di pesisir Semarang, yang mewakili daerah industri, pertanian dan pemukiman. Hasil penelitian menunjukkan nilai parameter fisika-kimiawi airtanah (pH, suhu, salinitas) mempunyai variasi yang rendah. Ke 10 sampel yang diamati menunjukan warna, rasa dan bau dalam air sampel. Hasil rata-rata kandungan heptaclor adalah 0.023-0.055 µg L-1 dan enfrin adalah tidak terdeteksi sampai 0.648 µg L-1. Hasil perbandingan kandungan pestsida organochlorine dalam aqifer dangkal di pesisir Semarang dengan WHO dan IWQS menunjukan bahwa air pada aqifer di Semarang termasuk terkontaminasi pestisida organochlorine dan membuktikan telah ditemukannya pestisida organochlorine pada aqifer dangkal di wilayah pesisir Kota Semarang.Kata kunci: aqifer dangkal, organochlorin pestisida, wilayah pesisir The present study was conducted to assess psycho-chemical parameters and the level of organochlorine pesticides contamination in shallow aquifer of Semarang city coastal areas. Ten samples of groundwater were collected in different sites of Semarang coastal i.e. industrial, agriculture and settlement areas. The results indicated that low variation existed among some physico-chemical parameter (pH, temperature, salinity). In the colors, taste and odor of 10 water sample were also investigated. Mean values found in positive samples were heptachlor, ranging from 0.023 to 0.055 µg L-1, whereas endrin, ranging from undetected to 0.648 µg L-1. Compare to World Health Organization (WHO) limits and Indonesian Drinking and Domestic Water Quality Standard for Ground Water (IWQS) showed that this study proved the presence of organochlorine pesticides contamination of some shallow aquifer supplies in the coastal areas of Semarang city. Key words : shallow aquifer, organochlorine pesticides, coastal area

Relationship Between Stage of Gonad Maturity and Level of Osmotic Work of Sea Cucumber, Paracaudina australis Widianingsih Widianingsih; Muhammad Zainuri; Sutrisno Anggoro; Hermin Pancasakti Kusumaningrum; Retno Hartati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 1 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

The holothuroid spawning can be succesful depend on varoius factors such as salinity, temperature, primary productivity and other physiological adaptation toward environment influences. The salinity fluctuation is one of the environment factors, which influence and can cause various osmotic pressure of coelomic fluid of Holothoroid and also gonad maturity stage. Holothuroid (sea cucumber), Paracaudina australis, is overexploited in Kenjeran waters, Surabaya, East Java. The study was aimed to reveal relationship between gonad maturity stage and level of osmotic work of sea cucumber P. australis. Fifty samples of P. australis were collected monthly. Coelomic fluid samples of approximately 200–1000 µl were obtained from all samples using disposable insulin syringes. There were 37 holothuroids classified in the late maturity (stage 5), 74 holothuroid in the earlier maturity stage (stage 1). Gonad maturity stage 5 (late maturity) could be reached on December 2016. Contrasly, the gonad maturity stage 1 (earlier maturity) was found on August 2016. The analysis of coelomic fluid showed that the average value of level of osmotic work ranged 70 to 91 mOsm/L H2O in the earlier gonad maturity stage. Contrasly, in the late maturity, the average value of level of osmotic work ranged 118 to 156 mOsm/L H2O. There is relationship between gonad maturation stage and level of osmotic work. From stage of gonad maturity 1 to 5, there were upward trend level of osmotic work in coelomic fluid of P. australis.

Uji Bioaktivitas Ekstrak Batang Tumbuhan Benalu Mangrove (Cassytha filiformis) : II. Uji Anti Bakteri Subagiyo Subagiyo; Wilis A Setyati; Ali Ridlo
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 10, No 1 (2005): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Benalu adalah tumbuhan epifit parasit yang hidup menempel dan menghisap makanan dari tumbuhaninangnya. Sehingga benalu yang hidup pada tumbuhan mangrove mempunyai kemampuan khusus untuk beradaptasi terhadap senyawa-senyawa yang dibentuk oleh tumbuhan mangrove. Kondisi ini memungkinkan untuk ditemukannya jenis-jenis senyawa baru yang mempunyai potensi sebagai senyawa yang mempunyai nilai di bidang farmakologi dan agrokimia, diantaranya adalah anti bakteri Penelitian ini bertujuan untuk melakukan pengujian secara kualitatif dan kuantitaif bioaktivitas antibakteri dari fraksi-fraksi ekstrak batang C. filiformis. Penelitian dilakukan dengan metode experimental. ekstraksi dan pemisahan menggunakan pelarutkloroform-metanol (20:1). Fraksi-fraksi yang diperleh diuji bioaktivitas antibaktri menggunakan metodepaper disk. Ada 5 variasi konsentrasi fraksi yang diuji yaitu 50 ug/disk, 10 ug/disk, 5 ug/disk, 1 ug/diskdan 0,5 ug/disk. Berdasarkan hasil uji bioaktivitas antibakteri dari ekstrak batang benalu mangrove (C.filiformis) dapat disimpulkan bahwa ekstrak C. filiformis mempunyai potensi sebagai sumber metabolit antibakteri. Dalam penelitian ini semua fraksi tidak menunjukan aktivitas antibakteri terhadap bakteri V. parahaemoliticus dan S. aereus. Aktivitas antibakteri ditunjukan oleh semua fraksi terhadap bakteri uji V. harveyi dan V. anguilarum. Sedangkan aktivitas anti bakteri terhadap E. coli hanya ditunjukan oleh fraksi 4 dan fraksi 5.Kata kunci: Antibakteri, ekstrak C filiformis, benalu mangroveMistletoe as arboreal parasitic plant, hold and absorb their nutrient from host. During nutrient absorption,all of metabolites (include secondary metabolites) which host producing will be absorbed too. For thatmistletoe must have capability to developing strategies to adapt. The one of adaptation strategies is creating bioactive compounds, and predicted that between bioactive compounds have antibacterialactivity. The aim of this research were to antibacterial test of C filiformis stem extract. C filiformis wasextracted in chloroform-methanol (20:1). The fractions were examined to antibacterial activity using paper disc method. There are 5 variation concentration, which tested, 50 ug/disc, 10 ug/disc, 5 ug/disc, 1 ug/disc and 0,5 ug/disc. The result showed that all of fractions of stem extract of C. filiformis were able to inhibit the growth of both V. harveyi dan V. anguilarum, but not toward V. parahaemoliticus and S. aereu, while fraction 4 and fraction 5 were able to hold the growth of E. coli.Key words : Antibacteria, stem extracts of C filiformis, mangrove mistletoe

Tembaga (Cu) Menurunkan Kandungan Pigmen dan Pertumbuhan Mikroalga Merah, Porphyridium cruentum (Effect of Copper on Pigments Content and Growth of Red Microalgae, Porphyridium cruentum) Reza Hafiz Pranajaya; Ali Djunaedi; Bambang Yulianto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 19, No 2 (2014): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Logam berat tembaga (Cu) merupakan salah satu pencemar yang paling mengkhawatirkan di wilayah pesisir dan lautan. Berbagai metode telah banyak dikembangkan untuk mengatasi dan mengurangi pencemaran logam berat, baik secara fisika, kimia dan biologi. Masalah teknis dan biaya yang mahal menyebabkan manusia menggunakan cara biologis (bioremediasi). Salah satu diantaranya menggunakan mikroalga Porphyridium cruentum. Penelitian bertujuan untuk mengetahui pengaruh tingkat konsentrasi logam berat Cu terhadap kandungan klorofil, pigmen fikobiliprotein dan pertumbuhan mikroalga P. cruentum. Bibit mikroalga diperoleh dari Balai Besar Pengembangan Budidaya Air Payau Situbondo. Rancangan penelitian menggunakan metode eksperimental laboratorium. Konsentrasi logam berat Cu yang digunakan adalah 0 ppm sebagai kontrol, 1, 2, 3 dan 4 ppm. Logam berat Cu dianalisa menggunakan AAS dan pigmen (klorofil dan fikobiliprotein) menggunakan spektrofotometri UV-Vis.Hasil yang didapat menunjukkan bahwa logam berat Cu dengan konsentrasi yang berbeda memberikan pengaruh yang sangat nyata (P<0,01) terhadap kandungan pigmen (klorofil dan fikobiliprotein), BCF dan pengaruh nyata (P<0,05) terhadap laju pertumbuhan P. cruentum. Semakin tinggi logam berat Cu membuat laju pertumbuhan, kandungan pigmen (klorofil dan fikobiliprotein), dan BCF pada P. cruentum semakin menurun. Prosentase penyerapan logam berat Cu tertinggi sebesar 13,1 % (1 ppm), 8,2 % (2 ppm), 6,9 % (3 ppm), dan 2,6% (4 ppm). Hasil penelitian ini menunjukkan bahwa P. cruentum berpotensi sebagai bioremediator. Kata kunci: Porphyridium cruentum; pigmen; klorofil; fikobiliprotein; pertumbuhan; tembaga Copper (Cu) is one of heavy metals and the most pollutant at seawater ecosystem. Various methods have been developed to reduce heavy metal pollution with in physics, chemistry and biology method. Technical problems and high costs cause human use biological method (bioremediation). One of them used microalgae Porphyridium cruentum.This study aims to find out the influence of copper exposure levels on chlorophyll, pigment Phycobiliproteins, and the growth of microalgae Porphyridium cruentum. P. cruentum stock was collected from Main Center Brackish Water Aquaculture Development, Situbondo. The research design this study used a experimental laboratory. Concentrations of heavy metals Cu used are 0 ppm as control, 1 ppm, 2 ppm, 3 ppm, and 4 ppm. Heavy metals Cu analysised by AAS and measurent pigments (chlorophyll and phycobiliproteins) performed by spectrometric UV-Vis. The results showed that heavy metals Cu with different concentrations give a very effect influence (P<0.01) to pigment (chlorophyll and phycobiliproteins) content, BCF, and effect influence (P<0.05) to growth. The high Cu resulted decrease to growth of microalgae, cell density, pigments (chlorophyll and phycobiliproteins), and bio concentration factor Porphyridium cruentum. The highly percentage absorption of heavy metals Cu at 13.1% (1 ppm), 8.2% (2 ppm), 6.9% (3 ppm), and 2.6% (4 ppm). The results suggest that P. cruentum has the potential as bioremediator. Keywords: Porphyridium cruentum; chlorophyll; phycobiliproteins; growth; copper

Skrining Bakteri yang Berasosiasi dengan Spons Jaspis sp. Sebagai Penghasil Senyawa Antimikroba Hermawaty Abubakar; Aris Tri Wahyudi; Munti Yuhana
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 1 (2011): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Organisme bentik laut seperti spons, seringkali hidup berasosiasi dengan bakteri yang menghasilkan senyawa antimikroba. Penelitian ini bertujuan untuk mengetahui kemapuan antagonis isolat-isolat bakteri yang berasosiasi dengan spons Jaspis sp. terhadap beberapa bakteri patogen, dengan metode skrining secara kualitatif. Sebanyak 32 (45,71%) dan 20 (29,41%) isolat yang berasal dari bagian mesohyl dan permukaan Jaspis sp. menunjukkan kemampuan antimikroba, karena mampu menghambat pertumbuhan Staphylococcus aureus, Vibrio harveyii, Escherichia coli, Pseudomonas aerogenosa, EPEC K-11, Candida albicans, and C. tropicalis. Uji fenotipik dilakukan pada beberapa isolat dengan aktivitas antimikroba terbaik, yaitu SAB E-8, SAB E-33, SAB E-35, SAB E-38, SAB E-40 dan SAB S-43. Hasil pewarnaan Gram menujukkan isolat SAB E-8, SAB E35, and SAB E-40 adalah Gram negatif, sedangkan isolat SAB E-33, SAB E-38, and SAB S-43 adalah gram positif yang dilanjutkan dengan identifikasi parsial (pengecatan gram dan uji katalase) untuk kelompok Bacillus.Kata kunci: Bakteri, Asosiasi, Jaspis sp., antimikroba Living benthic marine organisms such as sponges are frequently assosiated with as bacteria that may be produce antimicrobial compounds. This study aims to determine antagonistic of bacterial isolates that associated sponge Jaspis sp., with a qualitative screening method. Screening of bacteria from marine sponge Jaspis sp. which have bility to produce antibacterial subtances was investigated. There are 32 (45,71%) and 20 (29,41%) isolates from mesohyl and surface sponge respectively. Those isolated bacterial showed the antibacterial activity against Staphylococcus aureus, Vibrio harveyii, Escherichia coli, Pseudomonas aerogenosa, EPEC K-11, Candida albicans, and C. tropicalis. However, use of a few additional simple phenotypic tests for those isolate can be used to differentiate among isolates. The simple phenotypic test divided two ways based on staining gram. Gram negative bacteria were desingned SAB E-8, SAB E-35, and SAB E-40 and gram positive bacteria were desingned SAB E-33, SAB E-38, and SAB S-43. Parsial identification that directed to Bacillus was used for positive gram bacteria, involve gram staining, endospora staining and katalase test. Key words: Bacteria, Assosiation, Jaspis sp, antimicrobe

Pertumbuhan dan Kemampuan Planula Karang Scleractinia AIveopora japonica Eguchi dalam Menempel Thamrin Thamrin
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 7, No 3 (2002): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Alveopora japonica Eguchi memproduksi tipe planula dalam bentuk normal dan abnormal (kembar). Kedua tipe planula-planula ini mampu menempel, melakukan metamorfosis dan tumbuh. Planula kembar mampu menempel sebagai mana planula normal bila disediakan substrat (substrat dari alam). Tipe planulaplanula kembar ini diperkirakan menempel sangat dekat dengan induknya. Akan tetepi, kecepaten penempelannya berbeda nyata antara berbeda substrat dan sebagian kecil planula menempel pada batu hitam (black shale) dan ubin keramik dalam 4 minggu. Kondisi permukaan substrat yang ditutupi oleh microorganisme tidak begitu penting dalam penempelan, dan tipe substrat sendiri mungkin jauh lebih penting menentukan keberhasilan planula-planula ini dalam penempelan.Kata kuncl: karang; Alveopora japonica; planula; substrate; penempelan Alveopora japonica Eguchi produced normal and abnormal (fused) type of planulae. Both planulae types were capable in settling, metamorphosing and growing. Branched planulae could settle in the same as normal planulae when there were introduced to an appropriate substrate type (the natural substret). These fused planulae types were predicted to settle very close to their parents. However, settlement rate was significantly different among different substrate types and small number of planulae settled on black shale and tiles during 4 weeks. The surface condition of substrate covered by organic coating may not be so important in settlement, and the substrate type itself might be much more essential for determining the successof these planulae in settlement.Keywords: coral; Alvopora japonica; planulae; substrate; settlement

The Effect of Various Salinity Level on the Growth and Characterization of Dunaliella sp Isolated from Jepara Waters Hermin Pancasakti Kusumaningrum; Endang Kusdiyantini; Triwibowo Yuwono; Joedoro Sudarsono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 3 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Dunaliella adalah salah satu biota dengan kandungan β-carotene cukup tinggi. Upaya optimalisasi produksi bcarotene pada Dunaliella berhadapan dengan beberapa masalah kultivasi, untuk mendapatkan species yang paling potensial. Hal ini terkait dengan keterbatasan pengetahuan karakteritik ecophysiologi. Alga hijau Dunaliella diketahui dapat tumbuh pada media dengan kandungan garam yang cukup tinggi, namun karena pemahaman characteristik yang keliru dapat menyebabkan identifikasi yang salah pada satu species dalam genus Dunaliella. Kultur laboratoris pada media microcosms berdasarkan salinitas telah dilakukan untuk mengetahui tingkat pertumbuhan dan karakterisasi Dunaliella sp. dari perairan Jepara. Hasil pengamatan menunjukkan bahwa Dunaliella sp. dapat beradaptasi pada salinitas 0 sampai dengan 30 ‰. Berdasarkan kepada perubahan warna pigmen Dunaliella sp. yang tidak menjadi merah pada media pemeliharaan sampai dengan 25 ‰, maka jenis yang dijumpai di Jepara mempunyai karakter dan secara taksonomis berafiliasi dengan Dunaliella viridis.Kata kunci: Dunaliella sp, Salinity, Growth, CharacterizationDunaliella is one the most enriched β-carotene eucaryotic organism known. The attempt to optimize bcarotene production from Dunaliella has faced with several problems related to its growth management,which was suspectedly unable to meet the needs of the cultured species. This is primarily because the ecophysiological characteristic affecting growth of Dunaliella have not been sufficiently understood. It wasknown that the halophilic species of the green alga Dunaliella was grown in concentrated salt solutions, but based on this characterization, some misnamed of species in genus Dunaliella also have arisen due to wrongcharacterization understanding. Laboratory cultures and mixed-species microcosms were used to asses the growth and characterization of Dunaliella sp. from Jepara Coastal Region with special emphasis on the several factors that affecting growth of organisms including salinity. The result showed that Dunaliella sp. could adapted to a variety of salt concentration from as low as 0.0 % to salt saturation of about 30 ‰. Based on its pigment colour that Dunaliella sp. doesn’t turn red in the growth on salinities up to 25 ‰, it can be characterized and affiliated taxonomically as Dunaliella viridis.Key words: Dunaliella sp, Salinity, Growth, Characterization

Morfometri dan Perkembangan Gonad untuk Pendugaan Ukuran Kematangan Seksual pada Rajungan (Portunus pelagicus) Ali Djunaedi; Sunaryo Sunaryo; Dyah Rahmawati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 13, No 1 (2008): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Dalam rangka pengelolaan sumberdaya rajungan, penelitian tentang morfometri dan perkembangan gonad telah dilakukan dengan tujuan untuk mengetahui pendugaan kematangan seksual rajungan (Portunus pelagicus). Pengukuran karakteristik morfblogi, seperti: lebar karapas, lebar capit, panjang capit, dan panjang daktilus yang dapat bergerak dan lebar abdomen serta pengamatan perkembangan gonad telah dilakukan. Estimasiukuran kematangan morfometri dilakukan dengan metode smooth spline. Sedangkan, estimasi ukuran kematangan fisiologi dilakukan dengan memplotkan data perkembangan gonad pada fungsi logistik. Hasil penelitian menunjukkan kematangan seksual morfometri dan fisiologi pada rajungan jantan maupun betina dicapai secara bersamaan yaitu pada rajungan jantan masing-masing pada ukuran lebar karapas 81 mm dan 86,36 mm dan pada rajungan betina masing-masing dicapai pada ukuran karapas 87 mm dan 91,25 mmKata kunci: Portunus pelagicus, morfometri, perkembangan Gonad, kematangan seksual

Polycyclic Aromatic Hydrocarbons (PAHs) in water of Al-Kahlaa River in Missan Province, Iraq Salih Hassan Jazza; Abdul Hussain Y. Al-Adhub; Hamid T. Al-Saad
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 1 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

The present study was performed to have knowledge of Polycyclic Aromatic Hydrocarbonspollution status in water. The samples were collected during two seasons (winter and summer 2012 ,2013) from four different stations) Al-Magideh, Treatment unit, Al-Husaichi and Al-Zubair) distributed along Al-KahlaaRiver in Missan province, in addition to reference station lies on the Tigris river before 25 Km from entering to Amara city. The concentrations of PAHs were determined in by using capillary Gas Chromatography. Results of the present study revealed that the total concentrations of PAHs in dissolved fraction ranged from 0.739 ng.l-1 in Reference station to 1.974 ng.l-1 in Treatment unit during winter, and from 0.300 ng.l-1 in Reference station to 1.125 ng.l-1 in Treatment unit during summer, while in the particulate fraction they varied from 0.79 ng.g-1 dry weight in Reference station to 24.42 ng.g-1 dry weight in Treatment unit during winter, and from 4.369 ng.g-1 dry weight in Reference station to 10.545 ng.g-1 dry weight in Al-Husaich during summer. It had been noticed that there were a predominance of high molecular weight PAHs on low molecular weight, while BaA/(BaA+Chr) ratio in water ranged from 0.218 to 0.804. InP/(InP+BghiP) ratio ranged between 0 and 0.578,whereas Fl/Pyratio ranged between 0.150 and 2 ,this give an indication of the origin of PAHs compounds in water which were mainly pyrogenic and few from them petrogenic. Keywords: polycyclic aromatic hydrocarbons, PAHs, water, pollution

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