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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 54 Documents
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Search results for , issue "Vol 12, No 3 (2020): December 2020" : 54 Documents clear
Morphological Variation of Blue Panchax (Aplocheilus panchax) Lives in Different Habitat Assessed Using Truss Morphometric Mustikasari, Diah; Suryaningsih, Suhestri; Nuryanto, Agus
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.26593

Abstract

Blue panchax (Aplocheilus panchax Hamilton, 1822) lives in broad ranges of habitat from open waters to closed waters, including at ex-tin mining pits in Bangka Island, Indonesia. Variable habitats might cause morphological variations due to different ecological factors. This study aimed to assess the morphological variation of blue panchax collected from different habitats using truss morphometric characters. Fish samples (70 individuals) were collected from abandoned ex-tin mining pits of different ages and a river in Bangka Island. Twenty-nine truss characters were analyzed using the Kruskal-Wallis test and post hoc with Dunn’s test . The results showed that almost all of the body parts of blue panchax found in ex-tin mining pits and rivers were significantly different (p-value < 0.05), except some truss characters of caudal peduncle. The post hoc of Dunn’s test showed a positive correlation between habitats and truss characters (adj. sig < 0.05). Some truss characters of killifish were different between ex-tin mining pits with different ages chronosequence, and some other characters were different between pits and river.  This study provides the first data about the morphological variation of blue panchax in ex-tin mining pits of different ages. The data is valuable as a scientific basis of further utilization of ex-tin mining pits in the areas.
Potential of Ecology based Agrotourism for Agricultural Education in Besur Village, Lamongan Regency Ifandi, Slamet; Rahma, Yustika Aulia
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25886

Abstract

Currently, agriculture has prospective potential to be developed as a tourist attraction, one of which is agricultural tourism with an ecological perspective. The objective of the research was to examine the potential of ecology-based agrotourism as a means for agricultural education in Besur Village, Lamongan Regency. The research applied a quantitative descriptive approach in the form of scoring, weighting, and ranking of the categories being assessed. The research data consisted of primary and secondary data. The data was collected using direct observation and in-depth interviews. The data analysis was carried out using for the feasibility of  agrotourism area. The results showed that the area of Besur Village was classified as suitable (potential) with zone I that had the highest KKA value (2.6) as the object of the agrotourism. Potential ecological aspects obtained analysis 36 species of food producing plants. The highest Importance Value Index (IVI) of plant species was  recorded for Oryza sativa spp. (83.76%) and Zea mays L. (27.11%). Analysis of the physico-chemical properties of soil resulted in moderate or sufficient fertility. Analysis of public perceptions obtained was 4.08 and classified as good for the development of agrotourism in Besur Village. The novelty of this research was to obtain of the potential for agrotourism management in Besur Village through an ecological approach based on the concept of Healthy Plant Management.
Biochemical and Histopathology Analysis of Liver Damage in Hypercholesterolemic Rats Induced by Tomato Extract Iswari, Retno Sri; Dafip, Muchamad; Rifa'i, Muhammad
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23337

Abstract

Hypercholesterolemia is a condition caused by high cholesterol consumption. Tomatoes is well known has cholesterol-lowering effects. However, high consumption of tomato shall be concerned especially prooxidant potential that may damage the organ, especially in liver. This study aims to understand the effect of hypercholesterolemic and tomato administration to the rat’s liver, which is monitored using aspartate transaminase (AST), alanine transaminase (ALT) and histological condition. A total 24 of 12-weeks-old male-rats divided into 4 groups, equally. The K1 as normal group consist of placebo-treatment rats; K2 group as hypercholesterolemia group induced with high-cholesterol diet and 2 ml of cholesterol; K3 group was hypercholesterolemia rats treated with 20 mg of atorvastatin; and the last is K4 group was cholesterol rats supplemented with 16 mg/ day of tomato extract. All samples were treated for 60 days. The highest levels of AST and ALT level was 76.39 U/L and 45.40 U/L, respectively, was found in K2. Then, K4 is not significantly different from K3 and K1 and significantly different from K2. The scoring results showed that all groups experienced damage in the form of parenchymal degeneration, fat degeneration and necrosis. From this study it can be concluded that tomato extract gives a protection to the liver from cholesterol-oxidation damage effect. The updating information about how tomato inhibite liver fattening. The liver condition probably can be considered as biomarker-related hypercholesterolemia and developed a diagnostic marker to prevent increases metabolic disorder in community.
Histopathological of White Rats Aorta Induced by High-Fat Feed After Administered by Neem Leaf Ethanolic Extract Jannah, Baiq Miftahul; Isdadiyanto, Sri; Sitasiwi, Agung Janika
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25494

Abstract

Neem (Azadirachta indica A. Juss) is one of the traditional medicines used by local people as antioxidants, anti-hyperlipidemia, anti-inflammatory, and treatment for other diseases such as heart disease. This study aimed to analyze the effect of neem leaf ethanolic extract on aortic wall thickness and aortic lumen diameter of white rats induced by high-fat feed. The male Wistar rats (Rattus norvegicus L.) with 2 months of age were used. This research was an experimental study with a Randomized Complete Block Design consisted of 6 treatments (P0: commercial feed, P1: high-fat feed and duck egg yolk 2.5 ml/200 g BW per oral, P2: P1 + 8 mg/200 g BW simvastatin, P3-P5: P1+ neem leaf extract of 75, 100, and 125 mg/200 g BW respectively) and 4 replications. Aortic preparations were made by the paraffin method and Hematoxylin-Eosin staining. Data were analyzed using ANOVA followed by Duncan Multiple Range Test with 95% confidence level. The result showed that the decrease in wall thickening and lumen narrowing was getting higher along with the increasing doses of ethanol extract of neem leaves. Based on the result of this research, it was found that the high doses of neem leaf ethanolic extract has the same ability with simvastatin to reduce aortic wall thickning and aortic lumen narrowing of white rats. This study is give a new information that the neem leaves can be used as an alternative medicine for cardiovascular disease.
Stability and Antibacterial Property of Polyherbal Mouthwash Formulated Using Local Ingredients Nafea, Juman; Edbeib, Mohamed; Notarte, Kin Israel R.; Huyop, Fahrul; Yaakub, Harisun
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25243

Abstract

The oral cavity is a home to more than 500 bacterial species. Although some of the oral bacteria are harmless, there are certain species that may cause oral plaques, bad breath, and mouth disease. Thus, maintaining a good oral hygiene is essential for a healthy mouth and body. The present study aimed to formulate a polyherbal mouthwash that may have antibacterial properties. Mouthwash formulations were prepared containing varying percentages of herbal extracts, with each formulation stored at 12 °C and 25 °C. Over the course of 12 weeks, the appearance and pH of the formulated mouthwash were measured. The mouthwash formulations maintained good homogeneity and color when stored at 25 °C, displaying lower pH level ranging between 3.71 and 4.85. Although the mouthwash stored at 12 °C maintained good homogeneity, a change in color in the formulation was evident and a more unstable pH readings were recorded. Antibacterial assay showed that mouthwash formulations stored at 25 °C had better inhibitory activity compared to those stored at 12 °C. Furthermore, mouthwash formulation containing (30% v/v) aleppo oak extract as the major ingredient conferred the greatest inhibition zone diameter (IZD = 10-18 mm) against salivary bacteria compared to formulations with (30% v/v) clove and (30% v/v) turmeric extracts as major ingredients. The best polyherbal mouthwash formulation in terms of inhibiting bacterial growth followed the 3:1:2 ratio for aleppo oak extract, clove extract, and turmeric, respectively. Therefore, the polyherbal mouthwash formulated in this study has the potential to be optimized and commercialized to antagonize growth of pathogenic oral bacteria.
Feed Resources Determination Based on Pollen Diversity in Trigona Bees (Trigona sp.) Colony Abas, Muachiroh; Sucianto, Eddy Tri
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.26603

Abstract

Trigona bees' food resources can be determined either directly based on flowering plants live closed to the nest or based on pollen diversity inside the nest. There is no study about Trogona bee's food resources determination based on pollen diversity inside the bee colony's nest. This study aimed to determine plant food resources based on pollen diversity found inside the Trigona nest. This research was conducted in Serang village, sub-district of Karangreja, Purbalingga Regency. Pollen samples were taken from flowering plants lives around the nest and those in the nest and then were prepared using the acetolysis method. The variables observed were pollen morphology, with parameters such as unit, shape, size, aperture, and ornamentation. The data obtained were analyzed descriptively. Based on pollen diversity, 43 species and 22 plant families were live around Trigonanest in Serang Village. Forty-one pollen types were found inside the Trigonas' nest, with 37 of them are identic to the pollen collected from flowers around the nest. It can be concluded that 37 species of flowering plants could be determined as food resources for the Trigona bee based on pollen diversity inside the nest. This research provides the first data about feed resources for Trigona bee in Serang Village based on pollen diversity. The results provide essential information about food resources, which is vital for the development of Trigona bee cultivation.
Comparison of Nutritional Content of Mangifera indica L. var. Wirasangka with Three Other Varieties in Tegal Regency Rahayu, Enni Suwarsi; Retnoningsih, Amin; Abdullah, Muhammad; Susanti, Priyanti
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.26728

Abstract

Mangifera indica L. var. wirasangka or wirasangka mango, a local plant in Tegal Regency, has a low population because it is rarely planted by people as its unknown nutritional content. To increase people's interest in planting, it is necessary to know the nutritional content of wirasangka mango. The aim of the study was to compare the nutritional content of wirasangka mango with three other varieties in Tegal Regency. This study used a one-factor completely randomized design. The factor was the mango variety which included six varieties, namely the varieties of wirasangka from three areas, okyong, tengkoeh, and golek. The variables observed were content of water, fiber, fructose, β-carotene, potassium and iron, as well as vitamin C and antioxidant activity. Normally distributed data were analyzed using One-Way Analysis of Variance and Least Significant Difference (LSD) test. Besides, the non-normally distribution data were analyzed using Kruskal Walis and continued with post-hoc test, i.e. Mann-Whitney. The results showed that the levels of water, dietary fiber, fructose, β-carotene, and iron were significantly different at the significance level of 0.1. Wirasangka mango has a higher fiber, and fructose content, but lower of β-carotene and iron content compared to okyong, tengkoeh and golek mangoes. The levels of vitamin C, potassium, and antioxidant activity of wirasangka mango were equivalent to the three other varieties studied. Thus the wirasangka mango has advantages which are expected to increase the interest of people in Tegal Regency to plant this variety. As a result, its population in the future will also increase.
Qualitative Tests of Secondary Metabolite Compounds in Ethanol Extract of Spirulina platensis from Karimun Jawa Sea, Indonesia Yuniati, Renni; Zainuri, Muhammad; Kusumaningrum, Hermin
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23153

Abstract

Spirulina platensis is a spiral green-blue algae that is abundant in the subtropical and tropical water bodies. S. platensis has several potentials to be used in the medical fields due to its wound healing and antitumor potentials. This research aimed to evaluate the secondary metabolite compounds contained in the ethanolic extract of S. platensis from Karimun Jawa Sea, Indonesia. The dried S. platensis was macerated and extracted using ethanol, then was filtered using Whatman filter paper. A series of tests has been done to determine the secondary metabolites in the ozonated oil using several chemistry tests and reagents in the ethanol extract of S. platensis. The tests found that S. platensis microalgae harvested from Karimun Jawa sea contains alkaloid, saponin, flavonoid, and quinone. As S. platensis from different region contains different substances, this research shows the substances contained in S. platensis from Karimun Jawa sea. With this research, we knew that S. platensis from Karimun Jawa islands contains alkaloid, saponin, flavonoid, and quinone which can be used as a medicinal ingredient.
Characteristics of DNA Barcodes from Three Thrixspermum Orchids Based on ITS2 Regions Rohimah, Siti; Ratnasari, Tri; Su’udi, Mukhamad
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23737

Abstract

Thrixspermum (T.) is one of the genus in Orchidaceae that has small flowers. Among species in this genus has a high homology and also has many synonyms. Identification using morphological characters can be constrained since Thrixspermum flowering time occurs in a very short period. This study aimed to conduct molecular-based identification of Thrixspermum orchids using DNA barcoding. This method applied molecular-based species identification technique using DNA sequences from genomic fragments that are considered fast, accurate, and consistent. The molecular markers used were Internal Transcribed Spacer 2 (ITS2), while the samples used were T. centipeda, T. lucidum, and T. angustifolium. BLAST results show that T. centipeda has a close relationship with T. centipeda from Malaysia (KX679332) with 99.79% percent identity, T. lucidum has a high homology with T. linusii (KX679333; 97.30%), while T. angustifolium has a high homology with T. triangulare (KX679348; 99.38%). There is a unique sequence that only T. lucidum and T. angustifolium have that distinguishes the two from other species. ITS2 can be recommended as a molecular marker for determining the Thrixspermum orchid barcode. The benefit obtained from this research is the DNA barcode sequences (ITS2) of Thrixspermum orchids would be very useful to enrich the plant barcodes database for further molecular taxonomy and biodiversity of orchid. 
Bifidobacterium longum, a Predominant Bifidobacterium in Early-life Infant Potentially Used as Probiotic Kusharyati, Dyah Fitri; Rovik, Anwar; Ryandini, Dini; Pramono, Hendro
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25895

Abstract

In early life, Bifidobacteria are reported as dominant bacteria in the human digestive tract. Bifidobacterium is potential as a probiotic. The probiotic property of Bifidobacterium is strain-specific. This study aimed to identify the Bifidobacterium (isolated from less than one-month-old healthy infant stool that potentially used as probiotic) based on the 16S rRNA gene and determining their similarities among Bifidobacteria. The probiotic-potentially Bifidobacterium was re-characterized by performing a Gram’s staining and catalase test. The DNA extraction process was followed by the 16S rRNA amplification using 27F-1492R primers. Sequence similarity was checked by using the BLAST program in the GenBank. The phylogenetic tree was constructed by using a neighbor-joining (NJ) method within the MEGA version 7.0 package. The 16S rRNA gene was presented at 1,500 bp length. Bifidobacterium strains have a 91.14-94.26 % sequence similarity to B. longum subsp. longum strain CCUG30698 which is considered as insufficient for species and genus identifications. However, those isolates could be assigned in a phylogenetic position. This present study suggested the B. longum as the dominant strain of Bifidobacterium in the gut of early-life infants which has potential as a probiotic and is considered as an ideal probiotic for human consumption. This study is useful as basic information for other related research, as well as its application in industrial or community service fields.

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