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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10 Tri Handayani Kurniati; Iman Rusmana; Ani Suryani; Nisa Rachmania Mubarik
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6448

Abstract

Pyrene degradation and biosurfactant activity by a new strain identified as Gordonia cholesterolivorans AMP 10 were studied. The strain grew well and produced effective biosurfactants in the presence of glucose, sucrose, and crude oil. The biosurfactants production was detected by the decreased surface tension of the medium and emulsification activity.  Analysis of microbial growth parameters showed that AMP10 grew best at 50 µg mL-1 pyrene concentration, leading to 96 % degradation of pyrene within 7 days. The result of nested PCR analysis revealed that this isolate possessed the nahAc gene which encodes dioxygenase enzyme for initial degradation of Polycyclic Aromatic Hydrocarbon (PAH). Observation of both tensio-active and emulsifying activities indicated that biosurfactants which produced by AMP 10 when grown on glucose could lower the surface tension of medium from 71.3 mN/m to 24.7 mN/m and formed a stable emulsion in used lubricant oil with an emulsification index (E24) of 74%. According to the results, it is suggested that the bacterial isolates G. cholesterolivorans AMP10 are suitable candidates for bioremediation of PAH-contaminated environments.How to CiteKurniati, T. H.,  Rusmana, I. Suryani, A. Mubarik, N. R. (2016). Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10. Biosaintifika: Journal of Biology Biology Education, 8(3), 336-343. 
Morphological Variation of Blue Panchax (Aplocheilus panchax) Lives in Different Habitat Assessed Using Truss Morphometric Diah Mustikasari; Suhestri Suryaningsih; Agus Nuryanto
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.26593

Abstract

Blue panchax (Aplocheilus panchax Hamilton, 1822) lives in broad ranges of habitat from open waters to closed waters, including at ex-tin mining pits in Bangka Island, Indonesia. Variable habitats might cause morphological variations due to different ecological factors. This study aimed to assess the morphological variation of blue panchax collected from different habitats using truss morphometric characters. Fish samples (70 individuals) were collected from abandoned ex-tin mining pits of different ages and a river in Bangka Island. Twenty-nine truss characters were analyzed using the Kruskal-Wallis test and post hoc with Dunn’s test . The results showed that almost all of the body parts of blue panchax found in ex-tin mining pits and rivers were significantly different (p-value 0.05), except some truss characters of caudal peduncle. The post hoc of Dunn’s test showed a positive correlation between habitats and truss characters (adj. sig 0.05). Some truss characters of killifish were different between ex-tin mining pits with different ages chronosequence, and some other characters were different between pits and river.  This study provides the first data about the morphological variation of blue panchax in ex-tin mining pits of different ages. The data is valuable as a scientific basis of further utilization of ex-tin mining pits in the areas.
Potency of Brown Sugar as a Nectar Substitute for Trichoglossus haematodus in Captivity Siti Nuramaliati Prijono; Rini Rachmatika
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i2.18730

Abstract

Trichoglossus haematodus (Linnaeus, 1771) is nectarivorous bird that feed on nectar as a source of carbohydrate. In captivity, it is not practical to provide a continued diet of nectar  from the flowers. Therefore, this study aimed to find other carbohydrate sources such as brown sugar as substitute nectar for T. haematodus. Twelve wild T. haematodus in four cages offered five types different brown sugar solution with different concentrations. Since brown sugar has low protein content, therefore the birds also  offered commercial baby biscuit  to meet the protein requirement.  The results showed that the birds like to consume all types of brown sugar solution with sugar concentration up to 40% . However, the siwalan brown sugar solution was the most favored by the bird. over the other type of brown sugar solutions (aren, coconut, sugar cane and regular commercial “palm” brown sugar).  Furthermore, the result showed that the birds prefer commercial baby biscuit was soaked in the brown sugar solution with concentration 20%. This study provides the information that the brown sugar solution based diet has potency as  an alternative carbohydrate source  to substitute nectar  for T. haematodus in captivity which  is more practical and can increase the survival rate in birds. 
The Impact of Lampenflora on Cave-dwelling Arthropods in Gunungsewu Karst, Java, Indonesia Isma Dwi Kurniawan; Cahyo Rahmadi; Tiara Esti Ardi; Ridwan Nasrullah; Muhammad Iqbal Willyanto; Andy Setiabudi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.13991

Abstract

The development of wild caves into show caves is required an installation of electric lights along the cave passages for illumination and decoration purposes for tourist attraction. The presence of artificial lights can stimulate the growth of photosynthetic organisms such as lampenflora and alter the typical cave ecosystem. The study was aimed to detect the effect of lampenflora on cave-dwelling arthropods community. Four caves were sampled during the study, 2 caves are show caves with the existence of lampenflora and 2 others are wild caves without lampenflora. Arthropods sampling were conducted by hand collecting, pitfall trap, bait trap and berlese extractor. Lampenflora comprises of algae (Phycophyta), moss (Bryophyta) and fern (Pteridophyta) grow mostly around white light lamps. Richness, diversity, and evenness indices of Arthropods are higher in caves with the existence of lampenflora compared to caves without lampeflora. This study clearly shows that the presence of lampenflora can increase Arthropods diversity and suppress dominancy of common Arthropods species in caves, also increasing the relative abundance of predators. This condition will shift the ecosystem equilibrium and lead to cave ecosystem destruction. The results of this study should be a scientific consideration for show cave development and management. Lampenfloras have to be removed from all caves and preventive efforts should be taken to minimize their growth.
Keanekaragaman Burung di Desa Karangasem Kecamatan Wirosari Kabupaten Grobogan Jawa Tengah Margareta Rahayuningsih; Fajar Adi Purnomo; Bambang Priyono
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 2 (2010): September 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i2.1156

Abstract

The aim of this study was to measure the bird’s diversity at Karangasem Village, Wirosari District, Grobogan Regency mainly at the forest habitat, farm land and it settlements. The study was conducted on September until November 2008 used point count method. The data of birds species and its number analyzed by Shanon-Wiener species diversity index (H’), evenness index (E), dominance index (Di) and similarity index of Jaccardto describe the similar species of birds inter-habitat at Karangasem. The result indicated that there are 43 birds species of 22 families recorded at the location. About eight species among them are protected by the government, two among them included into CITES appendix II and one of them included into Vulnerable category of IUCN Red List. The birds diversity index and evenness index in the village of Karangasem ranges from 1.9 to 2.7 and 0.68 to 0.8. The highest and most dominant species are Collocalia linchi, Pycnonotus aurigaster, Lonchura leucogastroides and Lonchura punctulata. The analysis of Jaccard’s similarity index showed that the forests had more similarity to settlements than rice farming, which means there are high differences between habitats. There are differences in composition, structure, and featured bird species of the habitat, whichcaused low similarity index between the habitat ranges from 19.23% to 25.71%. Keywords: Birds diversity, point count, Karangasem
The Gonad Maturity of Female Osteochillus vittatus in the presence of Ascorbic Acid Nuning Setyaningrum; Sugiharto Sugiharto; Hexa Apriliana Hidayah
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9848

Abstract

The fish’s reproduction status is affected by both fed compositions and vitamins intake lead to determine improvement of eggs quality as well as fish production. The presence of Ascorbic Acid (AA) in the cultivation ponds, might accelerate female’s gonad maturation and so rematuration. The research aimed to determine: (1) Level gonad maturity of supplementation Ascorbic Acid; (2) Oocyts diameter; (3) Larvae survival rate. The research used experimental methods.The method was a completely randomized design (CRD) of 4 treatments and 4 replications. Treatments were supplementation of AA at different dosages of 0, 600, 1200 and 1800 mg/kg fish-fed ration.The gonad maturity level was analysed descriptively and oocyte diameter and larvae survival rate data were analysed by ANOVA. The result showed that supplementation of AA at a dosage of 1200 mg / kg fish-fed ration accelerated the process of gonad maturity, development of oocyt diameter and larvae survival rate as well as the viability of O. vittatus larvae at 90 rearing days. In this case, level IV gonad maturity was reached at 90 days which characterized by completed vitelogenesis process and oocyte diameter of 1.1 mm. Thus, this study is useful for aquaculture science by providing information on utilization of AA as food supplement in fish culture and also for fish farmer who wish to accelerate O. vittatus reproduction.
Growth Response of Sorghum bicolor (L.) Moench. Cultivars to Trivalent Chromium Stress Sri Kasmiyati; Santosa Santosa; Irfan Dwidja Priyambada; Kumala Dewi; Sucahyo Sucahyo; Rintawati Sandradewi
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5178

Abstract

One of heavy metal pollutants in the soil that can be absorbed by the sorghum is chromium (Cr). The study was conducted to determine the growth response of Cr3+ stress of sorghum cultivars. Two chemical compounds Cr3+ and 3 level concentrations were exposed to sorghum cultivars. The research was conducted in two separate experiments i.e. during seed germination and early seedling development stages. The parameters measured were radicle/root length, seedling length, fresh weight, dry weight, and stress tolerance index (STI) value. The results showed that Cr3+ either in form of CrCl3 or KCr(SO4)2 significantly reduced the seedling growth of sorghum cultivars. The growth responses of sorghum cultivars toward Cr3+ stress showed differences both on stage of the germination and early seedling. Based on the average of STI value, four sorghum cultivars (Badik, Keris, Keris M3 and Numbu) were classified as very strong tolerant, 4 cultivars (Hegari, Mandau, Sangkur and Gambela) were categorized as moderate tolerant, two cultivars (UPCA and Selayer) were weak tolerant, and 2 cultivars (Kawali and Batari) were sensitive ones, under stress condition of Cr3+.The results of this study are expected to provide the scientific basis of the physiological and tolerance responses of sorghum cultivars toward Cr3+ stress condition.How to CiteKasmiyati, S., Santosa, S., Priyambada, I., Dewi, K., Sucahyo, S., Sandradewi, R. (2016). Growth Response of Sorghum bicolor (L.) Moench. Cultivars to Trivalent Chromium Stress. Biosaintifika: Journal of Biology Biology Education, 8(1), 71-84.
Effectivity of Pedada Fruit (Sonneratia caseolaris) Extract to The Level of Sgot and Sgpt in Rat Treated by Paracetamol Induction Ratih Devi Lestari; R Susanti
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 1 (2015): March 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i1.3541

Abstract

The study was aimed to determine the effectiveness of pedada fruit extract as a hepatoprotector in the experimental rat that fed by  toxic dose of paracetamol. The total of 30 white rats (Wistar strain, two months age, and 150-200 g weight) were randomly divided into 5 groups. Group I (normal control) only given distilled water for 7 days). Group II (negative control) that given distilled water for 7 days and then treated by 270 mg/head single dose of paracetamol. Group III, IV, and V (treatment group) were given a pedada fruit extract at a dose of 28 mg/head/day, 56 mg/head/day, and 84 mg/head/day for 7 days and then treated by 270 mg/head single dose of paracetamol. On the 9th day of treatment, the blood samples were taken and were further measured for its SGOT and SGPT level using photometry enzymatic method. The result of LSD test on SGOT and SGPT data showed that III, IV, and V groups were not significantly different to the group I (p0.05). However, it significantly different with the group II (p 0.05). Data of SGOT showed that group IV were significantly different (p 0.05) with the group V. Whereas, the data of SGPT among groups III, IV, and V were not significantly different (p0.05). The result of linier regression test indicated that dose 28 mg/head was the most effective dose. It was concluded that pedada fruit extract was able to provide a hepatoprotective effects in rats that fed by toxic dose of paracetamol and most effective dose as a hepatoprotector was 28mg/head/day.Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak buah pedada sebagai hepatorotektor tikus putih yang diberi parasetamol dosis toksik.Sebanyak 30 ekor tikus putih (strain Wistar jantan berumur dua bulan dengan berat badan 150-200 g) dibagi secara acak dalam lima kelompok.Kelompok I (kontrol normal) diberi aquadest selama tujuh hari.Kelompok II (kontrol negatif),diberi aquadest selama tujuh hari dilanjutkan pemberian parasetamol 270 mg/ekor dosis tunggal.Kelompok III, IV, dan V (kelompok perlakuan) diberi ekstrak buah pedada pada dosis 28 mg/ekor/hari, 56 mg/ekor/hari, dan 84 mg/ekor/hari selama tujuh hari dilanjutkan pemberian parasetamol 270 mg/ekor dosis tunggal. Hari ke-9 darah diambil dan diukur kadar SGOT dan SGPT dengan metode fotometri enzimatik. Hasil uji LSD data SGOT dan SGPT menunjukkan bahwa kelompok III, IV, dan V tidak berbeda nyata terhadap kelompok I (p0,05), namun berbeda nyata terhadap kelompok II (p0,05). Data SGOT kelompok IV berbeda nyata (p0,05) dengan kelompok V. Data SGPT tidak ada perbedaan nyata (p0,05) antara kelompok III, IV, dan V. Hasil uji regresi linier, dosis 28 mg/ekor adalah dosis paling efektif.Disimpulkan bahwa ekstrak buah pedada mampu memberikan efek hepatoprotektor pada tikus yang diberi parasetamol dosis toksik dan dosis yang paling efektif sebagai hepatoprotektor adalah dosis 28 mg/ekor/hari.
Taxonomic Approach for Species Diversity of Yeasts and Yeasts-like Fungi through D1/D2 Region of Large Subunit Ribosomal DNA Sequences I Nyoman Sumerta; Atit Kanti
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11588

Abstract

The identification of yeasts or yeasts-like fungi and verify their diversity are principal aspect for bioindustry and ecosystem sustainability. Taxonomic approach provides identification tool to ensure the taxonomic position of yeasts and yeasts-like fungi which definitely set to utilization concerns. The aim of this study is to understanding the taxonomic position of yeasts and yeasts-like fungi from the distinctive of its sequences relationship. Yeasts and yeasts-like fungi strains were isolated through various culture dependent methods from natural resources samples of Karimun Besar Island, Province of Riau Islands, Indonesia. The identification process was performed through amplifying the accurate DNA-based in D1/D2 region of large subunit (26S) ribosomal DNA. As the result, a total of 85 isolates of yeasts and yeasts-like fungi were obtained with 16 closest related taxa through phylogenetic tree construction. Ascomycetous was the predominating group representing 91% of the total isolates sequences followed by Basidiomycetous (8%) and Zygomycetous (1%). The black yeasts (yeasts-like) known as Aureobasidium melanogenum was predominant species with represent to 54% of total isolates and present in particular habitat. Taxonomically, there are six isolates are represent to be novel taxa candidates which pretend to enhance genetic resources of yeasts and yeasts-like fungi especially from Indonesia. In addition, this information provides specific technique to reach specific yeasts or yeasts-like fungi species in nature by managing the sample collection and culture methods.
Bioinformatics Education in Greece: A Survey Efrosyni-Alkisti Paraskevopoulou-Kollia; Pantelis G Bagos
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.7257

Abstract

Bioinformatics is an interdisciplinary field, placed at the interface of Biology, Mathematics and Computer Science. In this work, we tried for the first time to investigate the current situation of Bioinformatics education in Greece. We searched the online resources of all relevant University Departments for Bioinformatics or relevant courses. We found that all the Departments of Biological Sciences include in their curricula courses dedicated to Bioinformatics, but this is not the case for Departments of Computer Science, Computer Engineering, or Medical Schools. Despite the fact that large Universities played a crucial role in establishing Bioinformatics research and education in Greece, we observe that Universities of the periphery invest in the field, by including more relevant courses in the curricula and appointing faculty members trained in the field. In order for us to “triangulate” we didn’t confine ourselves to online resources and descriptive statistics but we also included interviews so as to have a more spherical view of the subject under discussion. The interviews provided useful insights regarding the teaching methods used by bioinformatics tutors, their attitudes and the difficulties they encounter. The tutors mentioned also the material that they choose, the audience’s attraction techniques and the feedback they receive.

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