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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
 76   77   78   79   80 
Application of Arbuscular Mycorrhiza from Senaru Forest Rhizosphere for Gyrinops versteegii Germination and Growth I Gde Adi Suryawan Wangiyana; Sukardi Malik
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14396

Abstract

The aims of this research are to apply Senaru forest  rhizosphere on Kabupaten Lombok Barat West Nusa Tenggara as Mycorrhiza inoculants for Gyrinops versteegii germination and growth. Rhizosphere sample was taken from ten sampling spot on Senaru forest between Latitude: 08o18.808’ S – 08o19.174’ S and Longitude: 116o24.138’ E – 116o24.181’E. This study employed Factorial Experiment Design with 2 Factor including: Medium Composition (M) and Mychorizza Inoculant (I). There were 5 media composition: M1 (sand), M2 (soil:sand = 1:2), M3 (soil:sand = 1:1), M4 (soil:sand = 2:1), M5 (soil). There were 2 types of Inoculation: I1 (without inoculant) and I2 (innoculant from senaru Rhizosphere). Growth parameters observed in this study were: germination percentage, stem length, stem diameter and root colonization. Germination percentage of G. versteegii seeds in all growth media are below 60 % which could be classified as low germination rate. Also germination from media without rhizosphere is higher than germination from media with rhizosphere. On the other hand, G. versteegii growth on rhizosphere media is slightly higher than growth of G. versteegii on media without rhizosphere based on stem diameter and length measurement. It tends that medium composition with higher sand proportion tended to gives better germination and growth rate of G. verteegii. Myorrhiza colonization on G. versteegii root was higher in media with rhizosphere addition. It could be concluded that Application of Senaru rhizosphere containing Mycorrhiza increases G. versteegii growth but not its germination percentage. This research enrich knowledge in biological science about asociation of mycorrhiza with G. versteegii especially on its growth and germination
Ketertarikan Bactrocera carambolae (Diptera: Tephritidae) pada Senyawa Volatil Olahan Limbah Kakao Dyah Rini Indriyanti; Edhi Martono; Andi Trisyono; Witjaksono -
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 1 (2013): March 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i1.2570

Abstract

Tanaman kakao (Theobroma cacao) menghasilkan biji. Biji ditutupi oleh pulp pada saat dipanen, kemudian difermentasi selama tiga hari untuk diambil biji kakao dan dihasilkan limbah cair. Penelitian ini bertujuan untuk menguji ketertarikan lalat buah pada olahan limbah kakao dan mengidentifikasi senyawa kimia volatil yang menarik lalat buah. Penelitian dilakukan di laboratorium, dimulai dari pengolahan limbah kakao, pengujian ketertarikan B. carambolae pada limbah dan mengidentifikasi senyawa volatil olahan limbah kakao. Hasil penelitian menunjukkan bahwa jantan dan betina B. carambolae tertarik pada limbah. Olahan limbah kakao berisi berbagai senyawa volatil yakni: amonia; etil-2-hidroksi propanoat ; 7-dodesenil asetat; senyawa asetamida; 3,5-dihidroksi-2-metil-5,6-dihidropiran; hidroksi metilfurfurol; dan derivat-1-undekuna.The cocoa plants (Theobroma cacao) produce seeds. The seeds are covered by pulp when it is harvested. Then these seeds were fermented for three days to transform them becoming cocoa seeds and the fermentation would produce liquid wastes. The study aims to test the attractiveness of fruit flies to the processed liquid cocoa wastes and to identify the chemical compounds which attract fruit flies. The study was conducted in the laboratory, beginning from the processing of cocoa wastes, the testing of the attractiveness of B. carambolae to wastes and to identify the chemical compounds of the processed cocoa wastes. The result showed that males and females B. carambolae were attracted to the wastes.The processed cocoa wastes contain various chemical compounds, i.e. ammonia; ethyl-2-hydroxy propanoate; 7-dodecenyl acetate; acetamide compounds; 3,5 dihydroxy-2-methyl-5,6-dihydropyrane; hydroxyl methylfurfurol; and 1-undecyne derivates.
Immunomodulatory Effectiveness of Aqueous Obat Pahit Extract of Lingga Malay Ethnic on White Rats (Rattus novergicus) Fitmawati Fitmawati; Rodesia Mustika Roza; Nery Sofiyanti; Isnaini Isnaini; Febrian Lailatul Fitri; Desi Paramita; Awal Prichatin Kusumo Dewi
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10496

Abstract

Obat pahit has been generally known and believed by Lingga Malay society as anti-aging agent. However, the study of Obat pahit is not scientifically proven. This research was aimed to prove immunomodulatory ability of Obat pahit potion from Lingga, Riau Archipelago. This study used white rats as an animal modelling, and Staphylococcus aureus as bacteria tester. The rats had been treated with aqueous Obat pahit extract from three TMPs on dose scales of 0.09, 0.18 and 0.27 mL/200g of body weight through oral administration for 7 days. Furthermore, on the 8th days, the experiment animals were injected by the preparation of bacteria tester through intraperitoneal administration in the amount of 0.5 mL/200 gram of body weigth and subsequently incubated for 1 hour after the injection. There were 2 observed parameters on this study, i.e efectivity and capacity of phagocytosis by leukocytes. The observation of leukocytes-phagocytocis activity was carried out by making a smear preparat samples of peritoneum fluid from rats. After the observation under microscope on a magnification of 100 times. The result was obtained the Obat pahit from Kalan PMT swere more effective on dose 2, while from SP4 and Linau TMPs were much more effective on dose 1. It is therefore, using these data of the results, the advanced doses scale of this Obat pahit would not be necessary. Obat pahit potion from Malay Lingga Malay Ethnic could become raw materials of immunomodulatory herbal medicine based on traditional knowledge. It also potentially as a standardized herbal.
Ethnotaxonomical Study of Mole Crab (Crustacea:Hippoidea) on Coastal Community of Cilacap Dian Bhagawati; Sutrisno Anggoro; Mohammad Zainuri; Lachmudin Sya'rani
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6491

Abstract

Local wisdom is a cultural heritage that needs to be preserved. The Cilacap’s coastal communities have traditional taxonomy knowledge that hasn’t been informed, especially about mole crab. This study aimed to explore the ethnotaxonomy knowledge of sand crabs (Crustacea: Hippoidea) in Widarapayung coastal communities. The purposive snowball sampling was used for the method, followed by observation and semi-structured interviews with 30 respondents. Data were analyzed descriptively based on observations and interviews. The results showed that the coastal communities in Widarapayung tourist areas could be divided into five groups, namely catchers, collectors, processors, traders, and consumers. The catcher was able to recognize and describe three types of sand crabs based on the shape of the body and their catchment areas. Captured crabs had the local name of ‘yutuk jambe’ (Emerita emeritus Linnaeus 1767), the appellation was based on carapace shape that resembled the shape of areca fruit; ‘Yutuk bathok’ (Hippa adactyla Fabricius 1787), had the carapace shape resembling a coconut shell, and ‘yutuk Kethek’ (Albunea symmysta Linnaeus 1758), had ornaments on the carapace surface similar with a monkey face, there were dense setae on the edge of the carapace, spiny long antennas and aggressively pinch when captured. The knowledge and skills of sand crabs classification and local name entitlement acquired by the people were in accordance with the Berlin’s model. How to CiteBhagawati, D., Anggoro, S., Zainuri, M. Sya’rani, L. (2016). Ethnotaxonomical Study of Mole Crab (Crustacea:Hippoidea) on Coastal Community of Cilacap. Biosaintifika: Journal of Biology Biology Education, 8(2), 222-230.
Carotenoid Production by Rhodosporidium paludigenum Using Orange Peel Extract as Substrate Renna Eliana Warjoto; Jennifer Jennifer; Bibiana Widyawati Lay
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24867

Abstract

Carotenoids are fat-soluble pigments that have various benefits in health and beauty. The demand and market of carotenoids are increasing; thus, the faster and cheaper biological production of these pigments using microorganisms is desired. In this study, the optimum medium pH and nitrogen concentration for carotenoid production by Rhodosporidium (R.) paludigenum using orange peel extract as substrate were determined. The soluble sugars from orange peels were extracted using distilled water at 100 °C. The yeast inoculum was cultivated in Yeast Peptone Dextrose (YPD) media before fermentation. The independent variables in the fermentation factorial design included initial medium pH (5; 6; and 7) and nitrogen concentration (0; 1; and 1.75 g/L urea in the medium). The decrease in reducing sugar concentration and the increase in biomass dry weight during fermentation demonstrated carbon source consumption by R. paludigenum for growth and carotenoid production. On the sixth day of fermentation (D6), the culture was harvested for the total carotenoid extraction and determination. The highest total carotenoid yield (107.63 µg/g) was achieved by the treatment group with an initial medium pH of 6 without nitrogen supplementation. This result indicated the orange peel extract potential as a substrate for carotenoid production using R. paludigenum as a workhorse. This was the first reported research in natural carotenoid generation using R. paludigenum (strain InaCC Y-236) locally isolated in Indonesia as the producer and orange peel extract as the substrate. Thus, this research would enrich the microbial carotenoid exploration and waste valorization to value-added products at the same time. The study can then be developed further and upscaled for industrial applications. 
Repellent Activity of Waste Extract from Two Local Medicinal Plant Against Rice Weevil (Sitophilus oryzae) Priyantini Widiyaningrum; Devy Candrawati; Dyah Rini Indriyanti; Bambang Priyono
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.17651

Abstract

In order to find natural and inexpensive bioinsecticides, the active compound of waste extract from two medicinal plants were analyzed.  Ethanolic extracts of Amomum cardamomum and Zingiber zerumbet were evaluated under laboratory conditions for their repellency against rice weevil (Sitophilus oryzae, L.). Four extracts concentration were tested in this research, i.e: 25, 50, 75 and 100%.  Efficacy was measured based on the response of rice weevil in preference tests using Y Olfactometer Tube, then the results were converted into the Percentage Repellency (PR) and Preference Index (PI). The PR data were analyzed using ANOVA, whereas PI values were analyzed descriptively. The result showed that the PR were significantly (LSD test; α 0.05) and revealed that waste extracts repel the rice weevil at 100% solution.  The highest PR was found at 100% solution in both extracts, however, statistically the repellency of A. cardamomum waste extract was higher than Z. zerumbet’s. Likewise, the PI of both extracts showed negative value at 75 and 100% concentration. This study concluded that extract of A. cardamomum and Z. zerumbet wastes have repellent effect against rice weevil and A. cardamomum repellency is better than Z. zerumbet. The findings of this study can provide a useful information for development of a rice weevil repellent extracted from waste product of medicinal plants that is more economically and environmentally friendly than synthetic insect repellents.
Mealworm Powder as Culture Media of Local Isolate Semarang Entomopathogenic Nematodes Priyantini Widiyaningrum; Minnathul Khasanah; Dyah Rini Indriyanti
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13874

Abstract

Many researchers confirmed that entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema can be cultivated in vitro using artificial media that containing animal nutrition. However, artificial media with insect component hasn’t been widely studied. This research aims to analyze the population of EPNs isolate of Semarang cultivated in mealworm powder media. Five doses of mealworm powder (Tenebrio molitor) were tested in this research, i.e: 0.5; 1.0; 1.5; 2.0 g.  Culture media using 1 g of mealworm larvae was used as control. The best treatment was further tested for its pathogenicity on Macrotermes sp. at seven levels of invective juveniles (IJs) : 0; 50; 100; 150; 200; 250; 300 IJs/mL. Each treatment was repeated five times. The EPNs population and termites mortality were analyzed using ANAVA, whereas pathogenic value was calculated using Probit analysis. The result showed EPNs population were significantly (LSD test; α 0.05), likewise on termites mortality. The EPNs isolate of Semarang optimally at 0.5 g mealworm powder and  pathogenicity against termites based on LD50 and LD90 values at 220 JI/mL and 410 JI/mL doses, respectively. In conclusion, this result  can be an alternative to mass cultivation of EPNs, in effort of development of local bioinsecticides. The findings of this study also inform farmers that EPNs can be easily cultivated using the simple and available materials.
Efektivitas Penambahan Elisitor Asam Jasmonik dalam Peningkatan Sintesis Senyawa Bioaktif Andrografolid pada Kultur Suspensi Sel Sambiloto (Effectiveness of Jasmonic Acid Elicitor Addition for Andrographolide Synthesis Induction of Sambiloto Culture) Noor Aini Habibah
Biosaintifika: Journal of Biology & Biology Education Vol 1, No 1 (2009): March 2009
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v1i1.42

Abstract

In this research, we have studied synthesis of improvement of andrographolid bioactive compound on cell culture of sambiloto by addition of jasmonic acid. The essential problems in this research are firstly, the effects of addition of jasmonic acid either can induce or not andrographolide synthesis improvement of cell culture of sambiloto and secondly, to observe the largest content of andrographolide in jasmonic acid concentrations. Meanwhile, the purpose of this research are to observe the functions of jasmonic acid elicitor for induction of andrographolide synthesis improvement of cell culture of sambiloto and to optimize jasmonic acid concentrations which can produce the largest andrographolide content. The independent variable is concentration of addition of jasmonic acid on cell culture and the dependent variable are the growth of cell suspension culture and andrographolide bioactive content. Experiment result show that the optimum medium of sambiloto cell consist of Murashige Skoog (1962) medium supplemented by 0,5 ppm kinetin and 2,4-D 5 ppm. The cell growth phases are the followings : lag phase at age of 0-5 days, exponential phase of 5-15 days, and stationary phase at age of longer than 15 days. The highest andrographolide was 4,66 x 10-2 reached in cell culture was supplemented with 10 µM jasmonic acid. Keywords : andrographolide, sambiloto cell suspension culture, jasmonic acid elicitor.
Retracted: Characterization of the Interaction between RIN13 (RPM1-Interacting13) and Sumo Proteins in Arabidopsis thaliana. This article has been retracted at the request of the author. The reason is due to poor quality of the publication, which may reflect poorly on the scientific quality of the authors. Venny Santosa; Mio Nagabuchi; Sachiko Okada; Katsunori Tanaka
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9487

Abstract

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Preliminary Study on the Single Nucleotide Polymorphism (SNP) of XRCC1 Gene Identificationto Improve the Outcomes of Radiotherapy for Cervical Cancer Devita Tetriana; Wiwin Mailana; Iin Kurnia; Mukh Syaifudin
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3949

Abstract

Cervical cancer is the most fatal disease among Indonesian women. In recognition of the substantial variation in the intrinsic response of individuals to radiation, an effort had been done to identify the genetic markers, primarily Single Nucleotide polymorphisms (SNPs), which are associated with responsiveness of cancer cells to radiation therapy. One of these SNPs is X-ray repair cross-complementing protein 1 (XRCC1) that is one of the most important genes in deoxyribonucleic acid (DNA) repair pathways. Meta-analysis in the determination of the association of XRCC1 polymorphisms with cervical cancer revealed the potential role of XRCC1 polymorphisms in predicting cell response to radiotherapy.Our preliminary study with real-time polymerase chain reaction (RT-PCR) showed that radiotherapy affected the XRCC1 gene analyzed in blood of cervical cancer patient. Other published study found three SNPs of XRCC1 (Arg194Trp, Arg280His, and Arg399Gln) that cause amino acid substitutions. Arg194Trp is only SNPs that associated with high risk of cervical cancer but not others. Additionally, structure and function of this protein can be altered by functional SNPs, which may lead to the susceptibility of individuals to cancers. Anotherstudy found G399A polymorphisms. We concluded that SNP of this DNA repair genes have been found to be good predictors of efficacy of radiotherapy.Kanker serviks adalah penyakit yang paling fatal pada perempuan di Indonesia. Untuk memahami variasi substansial respon intrinsik individual terhadap radiasi, suatu usaha telah dilakukan untuk mengidentifikasi petanda genetik, terutama Single Nucleotide polymorphism (SNP), yang berkaitan dengan responsel kanker terhadap terapi radiasi. Satu dari SNP tersebut adalah X-ray repair cross-complementing protein 1 (XRCC1) yang merupakan satu dari gen paling penting dalam lajur perbaikan asam deoksiribonukleat (DNA). Meta-analysis dalam penentuan hubungan polimorfisme XRCC1 dengan kanker serviks menemukan adanya peranan potensial polimorfisme XRCC1 dalam memprediksi respon sel terhadap radioterapi. Studi awal kami menggunakan real-time polymerase chain reaction (RT-PCR) menunjukkan bahwa radioterapi mempengaruhi gen XRCC1yang dianalisis dalam darah pasien kanker serviks. Studi yang telah dipublikasi menemukan tiga SNP dari XRCC1 (Arg194Trp, Arg280His, dan Arg399Gln) yang menyebabkan substitusi asam amino. Arg194Trp merupakan satu-satunya SNP yang berkaitan dengan tingginya risiko kanker serviks, tetapi tidak pada yang lain. Di samping itu, strukturdan fungsi protein ini dapat berubah oleh SNP fungsional, yang mengarah ke kerentanan individu untuk menderita kanker. Studi lain menemukan polimorfisme G399A. Kami menyimpulkan bahwa SNP dari gen perbaikan DNA ini merupakan prediktor yang baik dari keberhasilan radioterapi.

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