cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Dr. Ir. Dyah Rini Indriyanti
Contact Email
biosaintifika@mail.unnes.ac.id
Phone
-
Journal Mail Official
biosaintifika@mail.unnes.ac.id
Editorial Address
-
Location
Kota semarang,
Jawa tengah
INDONESIA
Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
 78   79   80   81   82 
Production of Manooligomannan from Palm Kernel Cake by Mannanase Produced from Streptomyces Cyaenus Awan Purnawan; Yopi Yopi; Tun Tedja Irawadi
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.9201

Abstract

The increase of public attention to health has prompted researchers to look for new sources of functional food. Palm Cake Kernel (PKC) waste was abundant in Indonesia, Oligosaccharide has an important benefit for human health. Recently oligosaccharide is not only important as an artificial sweetener, but also as a functional food component. This study was aimed to produce oligo-mannan enzymatically from PKC waste using mannanase derived from of Streptomyces cyaenus isolates of indigenous Indonesia. The enzyme concentration was determined by enzyme activity assay while oligo-mannan content in the PKC was analyzed using TLC and HPLC. Mannanase enzyme activity of 1706 U/ml on the second day of agitation 200 rpm at a temperature of 30°C Hydrolysis of mannooligomannan by using mannanase produced by streptomyces cyaenus. The optimum mannanase enzyme activity obtained on day 2 with the value of the activity as much of 0.702 U/mL. The protein content of the 2nd day at an agitation speed of 150 rpm, 200 rpm, and 250 rpm, respectively, were 1783, 1950 and 2283 ppm. Streptomyces cyaenus is Indonesian original isolates potentially producing mannanase that can produce mannooligomannan.
Efek Antibakteri Ekstrak Daun Mimba (Azadirachta indica A. Juss) terhadap Bakteri Vibrio algynoliticus Secara In Vitro Uli Ayini; Siti Harnina B.; Titis Candra Dewi
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.3787

Abstract

Budidaya udang windu di Indonesia telah berkembang pesat. Salah satu kendala budidaya udang adalah penyakit Vibriosis yang disebabkan oleh bakteri Vibrio algynoliticus. Tujuan penelitian ini adalah untuk mengetahui efek antibakeri ekstrak daun mimba terhadap bakteri Vibrio algynoliticus. Penelitian ini menggunakan metode dilusi untuk mengetahui efek antibakteri ekstrak daun mimba terhadap bakteri Vibrio algynoliticus secara in vitro. Konsentrasi ekstrak yang digunakan (%) yaitu: 0; 2,5; 5; 7,5; 10; 12,5 dan sebagai kontrol terdiri dari kontrol positif, dan kontrol negatif. Pengumpulan data untuk menentukan MIC (Minimum Inhibitory Concentration) dilakukan dengan membandingkan kejernihan kultur di medium TSB 2% pada berbagai konsentrasi yang berbeda, dengan kontrol positif dan kontrol negatif. Penentuan MBC (Minimum Bacterisidal Concentration) dilakukan dengan melihat ada tidaknya dan jumlah koloni bakteri Vibrio alginolyticus yang muncul pada medium subkultur TSA 2% setelah inkubasi 24 jam. Hasil penelitian menunjukkan nilai MIC yaitu konsentrasi 5%, hal ini ditunjukkan dengan tabung yang mulai jernih. Nilai MBC ekstrak daun mimba terhadap bakteri Vibrio alginolyticus adalah konsentrasi 12,5% ditandai dengan sudah tidak munculnya  koloni bakteri Vibrio alginolyticus. Berdasarkan penelitian ini dapat disimpulkan bahwa ekstrak daun mimba dapat memberikan efek antibakteri terhadap bakteri Vibrio alginolyticus secara in vitro.Tiger shrimp cultivation in Indonesia has been growing rapidly. The main obstacle is the shrimp farming vibriosis disease caused by the bacterium Vibrio algynoliticus. The aim of this research was to determine the effects of neem leaf extract antibakeri against Vibrio algynoliticus. This study used a dilution method to determine the antibacterial effect of neem leaf extract against Vibrio algynoliticus bacteria in vitro. The concentration of the extract used (%): 0; 2.5; 5; 7.5; 10; 12.5 and as a control consisting of a positive control, and negative control. Data collection to determine the MIC (Minimum Inhibitory Concentration) was done by comparing the clarity of culture in TSB medium 2% on a variety of different concentrations, the positive control and a negative control. Determination MBC (Minimum Bacterisidal Concentration) was done by looking at the presence or absence and the number of colonies of bacteria Vibrio alginolyticus that appears in the subculture medium TSA 2% after 24 h incubation. The results showed that the value of MIC was 5%, as shown by the tube began to clear. Value of MBC of neem leaf extract against Vibrio alginolyticus was characterized by a concentration of 12.5% has been no emergence of bacterial colonies Vibrio alginolyticus. Based on the research, it concluded that neem leaf extract can provide antibacterial effect against bacteria Vibrio alginolyticus in vitro.
The Influence of Inducers on the Coltricia cinnamomea Laccase Activity and its Ability to Degrade POME Yohanes Bernard Subowo; Arwan Sugiharto
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.29660

Abstract

Some species of Basidiomycetes, specifically white rot groups, produce three ligninolytic enzymes, namely, Lignin Peroxidase (LiP), Manganese Peroxidase (MnP) and Laccase (Lac), which have low activity in degrading Palm Oil Mill Effluent (POME). The research objective was to obtain the data on the ability of the Coltricia cinnamomea to produce LiP, MnP, and Lac enzymes to degrade POME. This research also studied the effect of sucrose, alcohol, veratryl alcohol, CuSO4 and ZnSO4,as inducers. Isolates of Coltricia cinnamomea, which were stored in a PDA media at -20℃ were obtained from the Microbiology section of the Research Center for Biology (LIPI). Furthermore, the growth media used were DM, Bean sprout Extract (TE) and PDB. The result indicated that PDB is the most suitable growth media for the production of ligninolytic enzymes, because in this medium these enzymes showed the highest activity. It was also observed that sucrose increased the laccase activity by 40.80%. Furthermore, Coltricia cinnamomea was able to reduce the concentration of Poly R-478 by 60.74%, after the addition of ZnSO4. In addition, it degraded and decreased the color and COD of POME, by 72.63% and 91.19% respectively, after the addition of veratryl alcohol, and incubation for 10 days. Therefore, this fungus can be used to degrade POME in order to prevent environmental pollution. Coltricia cinnamomea has not been used for POME degradation. By using Coltricia cinnamomea, we  obtained new data regarding the activity of laccase and its ability to degrade POME. 
The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation Raudhah Mahfudhah; Kartini Eriani; Zainal Abidin Muchlisin
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22353

Abstract

The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds.
The Correlation Between Heavy Metal and Nutrient Content in Plecostomus (Pterygoplichthys pardalis) from Ciliwung River in Jakarta Dewi Elfidasari; Laksmi Nurul Ismi; Afina Putri Shabira; Irawan Sugoro
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.16248

Abstract

Most of the rivers’ quality in Indonesia is in highly-polluted status including Ciliwung River in Jakarta. The pollution can affect river ecosystem especially the rivers biota such as plecostomus species that is mostly found in Ciliwung River. The information about correlation between heavy metal and nutrition content in Plecostomus flesh still limited, so the result from this research can give the new information about that. The aims of this research is identifying the effect of the heavy metals towards the contents of protein and fat of plecostomus flesh from Ciliwung River. The analyses methods use X-Ray Fluoresence (XRF) Spectrometer, the analysis of protein using kjeldahl method, and fat analysis using extraction method. The result of this research showed that small-sized plecostomus contained the highest fat and protein compared to medium-sized and big-sized plecostomus. The assumption was the contents of heavy metal which can impede protein and fat absorption. Meanwhile, the content of carbohydrate in small-sized plecostomus was the lowest. The identified type of heavy metal using XRF method was Pb, Hg and Cd with the concentration was higher than the maximum standard (SNI) so that the flesh of plecostomus from Ciliwung River in Jakarta is categorized unsuitable to consumption
Potensi Ekowisata Danau di Kawasan Kamipang Kalimantan Tengah Decenly -; Tri Retnaningsih Soeprobowati; Fuad Muhammad
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i2.3104

Abstract

Penelitian ini bertujuan mengakaji potensi ekowisata di Kawasan Kamipang. Penelitian dilakukan melalui beberapa tahapan yaitu pra survei, survei dan analisis laboratorium. Pra survei dilakukan untuk menentukan 3 (tiga) stasiun pengukuran kualitas air dan 3 (tiga) posisi transek untuk analisis vegetasi. Survei meliputi pengukuran kualitas air danau, inventarisasi keanekaragaman ikan, analisis vegetasi, serta presepsi masyarakat lokal, pengunjung dan stakeholder. Analisis laboratorium yang meliputi identifikasi ikan dan tumbuhan. Potensi aspek abiotik dan sosial akan diuraikan secara deskriptif dan potensi aspek biologi dianalisis dengan pendekatan Indeks keanekaragaman ikan dan Indeks Nilai Penting (INP) untuk vegetasi. Berdasarkan hasil penelitian, diketahui bahwa potensi abiotik yaitu berupa danau-danau alami dengan kondisi hidrologi yang cukup baik (nilai BOD berkisar antara 2-4 mg/L dengan kekeruhan kurang dari 25 NTU) dan iklim tropis dengan tanah yang subur, sedangkan potensi biologi yaitu berupa keanekaragaman ikan dan tumbuh-tumbuhan di sekitar danau ditambah dengan keberadaan ikan dan tumbuhan yang termasuk dalam The IUCN Red List of Threatened Species 2013 dengan kategori Least Concern yaitu Ikan Peang (Channa maurilius), Balida (Notopterus chitala) dan Tumbuhan Kantong Semar (Nepenthes gracilis). Adapun potensi sosial berupa keunikan budaya dan kesenian tradisional. The study was aimed to evaluate the potential of ecotourism in Kamipang Region. The study was conducted through several stages of pre-survey, survey, and laboratory analysis. The pre-survey was conducted to determine three (3) water quality monitoring stations and three (3) transect positions for vegetation analysis. The survey included the measurement of lake water quality, the inventory of fish biodiversity, the vegetation analysis, and the perception of the local community, visitors and stakeholders. Laboratory analysis included the identification of fishes and plants. The potential of abiotic and social aspects were outlined descriptively and the potential biological aspects were analyzed using fish biodiversity index and vegetation important value index. The study showed the potential of abiotic aspects i.e. natural lakes with relatively good hydrological conditions (BOD value ranged between 2-4 mg/L and turbidity was less than 25 NTU) and tropical climate with fertile soil, whereas the biological potential comprised the diversity of fishes and vegetation around the lake along with the presence of fishes and plants that have been listed in the IUCN Red List of Threatened Species 2013 from the category of Least Concerned, i.e. Peang fish (Channa Maurilius), Balida (Notopterus Chitala) and Kantong Semar (pitcher) plants (Nepenthes Gracilis). The social potential was the uniqueness culture and the traditional arts.
Improving Students’ Scientific Reasoning and Problem-Solving Skills by The 5E Learning Model Sri Mulyani Endang Susilowati; Khaerul Anam
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.12022

Abstract

Biology learning in MA (Madrasah Aliyah) Khas Kempek was still dominated by teacher with low students’ involvement. This study would analyze the effectiveness of the 5E (Engagement, Exploration, Explanation, Elaboration, Evaluation) learning model in improving scientific knowledge and problems solving. It also explained the relationship between students’ scientific reasoning with their problem-solving abilities. This was a pre-experimental research with one group pre-test post-test. Sixty students of MA Khas Kempek from XI MIA 3 and XI MIA 4 involved in this study. The learning outcome of the students was collected by the test of reasoning and problem-solving. The results showed that the rises of students’ scientific reasoning ability were 69.77% for XI MIA 3 and 66.27% for XI MIA 4, in the medium category. The problem-solving skills were 63.40% for XI MIA 3, 61.67% for XI MIA 4, and classified in the moderate category. The simple regression test found a linear correlation between students’ scientific reasoning and problem-solving ability. This study affirms that reasoning ability is needed in problem-solving. It is found that application of 5E learning model was effective to improve scientific reasoning and problem-solving ability of students.
Relationship between Zingiberaceae Leaves Compounds and its Tyrosinase Activity Irmanida Batubara; Yuni Kartika; Latifah K Darusman
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6742

Abstract

The leaves of Zingiberaceae family has not been much explored its potential, especially as a skin lightening. The relationship between total anthocyanins, chlorophyll, carotenoids and tannins contents of Zingiberaceae leaves and tyrosinase activity were determined. Ten species of Zingiberaceae were separated by n-hexane and the residues were extracted with ethyl acetate. The total anthocyanins, chlorophyll, carotenoids, tannins contents and the activities of ethyl acetate extracts were determined by spectrometric method. The tyrosinase inhibition was determined by using L-tyrosine (monophenolase) and L-DOPA (diphenolase) substrates. The most active extract was the leaves extract of Zingiber purpureum which exhibited 82.86% for monophenolase inhibition and the leaves extract of Curcuma zedoaria which exhibited 90.20% for diphenolase inhibition. The correlation between carotenoids content and monophenolase inhibition was 52%, while between tannin content and diphenolase inhibition was only 15%. Therefore, among 10 leaves species of Zingiberaceae, Zingiber purpureum Roscoe and Curcuma zedoaria leaves are the most potential for tyrosinase inhibitors and can be developed as whitening agent.How to CiteBatubara, I., Kartika, Y. Darusman, L. K. (2016). Relationship between Zingiberaceae Leaves Compounds and its Tyrosinase Activity. Biosaintifika: Journal of Biology Biology Education, 8(3), 371-377. 
Effects of Moringa oleifera Leaves Powder in Fish Feed Toward Growth Rate and Health of Colossoma macropomum Safrida Safrida; Nonong Noviasyah; Khairil Khairil
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.22655

Abstract

Tambaqui (Colossoma macropomum) is one of freshwater fish that preferred by the community and also in high demand by fish farmers. It has a high selling value and easy to maintain but the growth is slow. One alternative that can be used to improve the fish growth is making rations with the addition of Moringa oleifera leaves. The aim of this study was to determine the effect of Moringa leaves powder on the growth rate and health of C. macropomum. This study used an experimental method with Completely Randomized Design (CRD) with 6 treatments and 4 replications. The data were analyzed using ANOVA Test in α =95%. The results showed that P3 with the addition of 30% Moringa leaves powder could increase the growth of Colossoma macropomum. An increase in fish body weight, width, and length was 40.1%, 21.6%, and 6.4% respectively.  Results of ocular, reflex, and defensive test showed that the fish were in healthy condition compared to their condition before acclimatization. The conclusions of this study is that the addition of Moringa leaves powder in an influential ration can increase the growth and maintain the health of C. macropomum. M. oleifera daily supplement can be used as a substitute for soy flour to promote growth and health of Colossoma macropomum in the seed phase. The addition of M. oleifera can be used as feed supplements to improve the growth and health of Colossoma macropomum.
Effects of Light Environments on Leaf Traits and Phenotypic Plasticity of Canna indica Yorianta Hidayat Sasaerila; Sakinah Sakinah; Nita Noriko; Risa Swandari Wijihastuti
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.30175

Abstract

Canna indica L (African arrowroot), is a beneficial, multi-use tropical perennial with a worldwide distribution, but relatively unexplored. This plant has the potential to be developed as a food crop in an intercropping system, utilizing idle land under commercial plantations such as rubber or teak. This study aimed to determine the best light-growing conditions for C. indica. A completely randomized design was used with growth light as the treatment consisting of 25%, 50%, and 100% of natural light, respectively. Leaf traits, growth characteristics, and phenotypic plasticity were used to measure C. indica’s response to different treatments. The results of this study showed that C. indica grown in low light has the best growth with increased height, leaf area, root and shoot dry weights, but decreased leaf thickness, which caused the increase in specific leaf area, leaf area ratio, and leaf weight ratio, but decreased root to shoot ratio. Based on leaf traits and biomass allocation, the phenotypic plasticity index was 0.23, a typical number for shade tolerant species. These findings were the first time to be reported for C. indica. For agroforestry practices, it can be recommended that C. indica be used as an intercropping plant under tree canopies.

Page 80 of 107 | Total Record : 1068

 78   79   80   81   82 

Filter by Year

2009 2023


Reset
Filter By Issues
All Issue Vol 15, No 3 (2023): December 2023 Vol 15, No 2 (2023): August 2023 Vol 15, No 1 (2023): April 2023 Vol 14, No 3 (2022): Desember 2022 Vol 14, No 2 (2022): August 2022 Vol 14, No 1 (2022): April 2022 Vol 13, No 3 (2021): December 2021 Vol 13, No 2 (2021): August 2021 Vol 13, No 1 (2021): April 2021 Vol 12, No 3 (2020): December 2020 Vol 12, No 2 (2020): August 2020 Vol 12, No 1 (2020): April 2020 Article-in-Press Vol 12, No 1 (2020): April 2020 Vol 11, No 3 (2019): December 2019 Vol 11, No 2 (2019): August 2019 Vol 11, No 1 (2019): April 2019 Vol 11, No 1 (2019): April 2019 Vol 11, No 3 (2019): Article-in-Press Vol 10, No 3 (2018): December 2018 Vol 10, No 3 (2018): December 2018 Vol 10, No 2 (2018): August 2018 Vol 10, No 2 (2018): August 2018 Vol 10, No 1 (2018): April 2018 Vol 10, No 1 (2018): April 2018 Vol 9, No 3 (2017): December 2017 Vol 9, No 3 (2017): December 2017 Vol 9, No 2 (2017): August 2017 Vol 9, No 2 (2017): August 2017 Vol 9, No 1 (2017): April 2017 Vol 9, No 1 (2017): April 2017 Vol 8, No 3 (2016): December 2016 Vol 8, No 3 (2016): December 2016 Vol 8, No 2 (2016): September 2016 Vol 8, No 2 (2016): September 2016 Vol 8, No 1 (2016): March 2016 Vol 8, No 1 (2016): March 2016 Vol 7, No 2 (2015): September 2015 Vol 7, No 2 (2015): September 2015 Vol 7, No 1 (2015): March 2015 Vol 7, No 1 (2015): March 2015 Vol 6, No 2 (2014): September 2014 Vol 6, No 2 (2014): September 2014 Vol 6, No 1 (2014): March 2014 Vol 6, No 1 (2014): March 2014 Vol 5, No 2 (2013): September 2013 Vol 5, No 2 (2013): September 2013 Vol 5, No 1 (2013): March 2013 Vol 5, No 1 (2013): March 2013 Vol 4, No 2 (2012): September 2012 Vol 4, No 2 (2012): September 2012 Vol 4, No 1 (2012): March 2012 Vol 4, No 1 (2012): March 2012 Vol 2, No 2 (2010): September 2010 Vol 2, No 2 (2010): September 2010 Vol 2, No 1 (2010): March 2010 Vol 2, No 1 (2010): March 2010 Vol 1, No 1 (2009): March 2009 Vol 1, No 1 (2009): March 2009 More Issue