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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
 80   81   82   83   84 
Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis Sri Puji Astuti Wahyuningsih; Manikya Pramudya; Sugiharto Sugiharto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4969

Abstract

Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains β-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1) and adding polysaccharide krestin before-after exposure (P3) had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 ± 0.523 M and 1.456 ± 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2) had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 ± 97.96 µM and 953.86 ± 328.16 µM respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., Sugiharto, S. (2016). Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology Biology Education, 8(1), 12-17.
Efek Interaksi Ragi Tape dan Ragi Roti terhadap Kadar Bioetanol Ketela Pohon (Manihot Utilissima, Pohl) Varietas Mukibat Tri Budi Kurniawan; Siti Harnina Bintari; R. Susanti
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i2.3783

Abstract

Ketela pohon (Manihot utilissima, Pohl) varietas mukibat berpotensi sebagai bahan baku bioetanol. Penelitian bertujuan untuk mengetahui efek interaksi ragi tape dan ragi roti terhadap kadar bioetanol ketela pohon mukibat dan menentukan interaksi terendah yang efektif menghasilkan bioetanol yang dapat terbakar. Sampel yang digunakan adalah ketela pohon mukibat umur 7 bulan dengan berat rata-rata 500 gram. Penelitian menggunakan Rancangan Acak Lengkap pola faktorial, yaitu konsentrasi ragi tape (0%, 0,3%, 0,6% dan 0,9%) dan konsentrasi ragi roti (0%, 0,3%, 0,6% dan 0,9%) dengan 3 kali ulangan. Parameter yang diukur meliputi jumlah sel khamir (data pendukung) dan kadar bioetanol (data utama). Data kadar bioetanol yang diperoleh dianalisis menggunakan anava dua arah dan dilanjutkan uji Duncan (DMRT) pada taraf kesalahan 5%. Hasil penelitian menunjukkan ada pengaruh interaksi ragi tape dan ragi roti terhadap kadar bioetanol ketela pohon mukibat p0,05. Konsentrasi ragi tape dan ragi roti yang tinggi menghasilkan kadar bioetanol yang tinggi. Interaksi ragi tape 0,6% dan ragi roti 0,3% (T2R1) merupakan interaksi terendah yang efektif menghasilkan kadar bioetanol yang dapat terbakar (36%). Interaksi ragi tape 0,9% dan ragi roti 0,9% (T3R3) menghasilkan kadar bioetanol tertinggi dengan rata-rata mencapai 49,8%.Cassava (Manihot utilissima, Pohl) var Mukibat is potential for bioethanol feedstock. The study aimed to determine the effects of the interaction of bread yeast and tape yeast on the concentration of mukibat cassava bioethanol and to determine the lowest effective interaction can produce flammable bioethanol. The samples used were mukibat cassava aged 7 months with an average weight of 500 grams. Research design was completely randomized design factorial, tape yeast concentration (0%, 0.3%, 0.6% and 0.9%) and the concentration of yeast bread (0%, 0.3%, 0.6% and 0.9%) with 3 replications. Parameters measured include the number of yeast cells (supporting data) and ethanol concentration (primary data). Bioethanol content data obtained were analyzed using two-way ANAVA and Duncan continued test (DMRT) at the level of 5% error. The results showed that no effect of the interaction of breads yeast and tape yeast on levels of cassava bioethanol p 0.05. The concentration of bread yeast and tape yeast yielded in high concentration of ethanol. The interaction of yeast tape 0.6% and 0.3% bread yeast (T2R1) gave the lowest effective interaction produced 36% ethanol. The interaction of tape yeast 0.9% and 0.9% bread yeast (T3R3) resulted in the highest ethanol concentration with an average reached 49.8%.
Ethno-ecology of Komplangan Field of the Bromo, Tengger, and Semeru Area in East Java:A Qualitative Approach Jati Batoro; Serafinah Indriyani; Bagyo Yanuwiadi
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.9193

Abstract

This research supports the sustainable environmental development, especially at Perhutani area. The objective of this ethno-ecological study was to know relationship between daily life of local people related to their agroforestry practices in edge of the forest belong to Perhutani at the landscape of Bromo Tengger Semeru-East Java. The Non-Timber Forest Products (NTFPs) showed the importance of the forest for the local people. This study covered the perception and conception traditional management system of environment by the local society and also impact of their activities. Data were collected by applying ethno-ecology research methods. Quality of data  were measured by means of participatory ethno-botanical appraisal (PEA) and some research methods included semi-structural and open discussion, in-deep interview, direct observation and plants identification. The results showed that development and management of the natural resources, in Komplangan agroforestry, were highly related to the plant conservation policy. The land use system was adapted from indigenous knowledge which consisted of holly sites (Pedanyangan), worship sites (Sanggar Pamujan), cemetery area and terrace. The conservation model and traditional knowledge in agricultural practices could be used as a model of komplangan area which should be taken into account as the key of biodiversity conservation. Traditional knowledge from these integrative studies will support the sustainable development of NTFPs.
Potential of Sodium Alginate in Sargassum sp. in Lotion Preparation to Treat Incision Wound in Mice Amelia Cahya Angelina; Ervia Yudiati; Delianis Pringgenies
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.22539

Abstract

Sargassum sp. is a known genus of brown seaweed whose dominant component is alginic acid or alginate. Various studies found that alginate having proinflammatory activity, so it may have potential to improve the wound healing process. This study aimed to obtain sodium alginate extract and to know the potency of the sodium alginate extract in incision wound treatment in mice abdomen. Lotion application was performed once a day in a span of five consecutive days. Observation on the wound was carried out to obtain data on blood coagulation, tissue reparation, and fibroblast development rate on the wound using histology. The results showed that the average yield from the Sargassum sp. extraction process was 31.3%. Quality control of the prepared lotion included organoleptic test; light brown coloration, lotion specific odor, semi- solid texture. The acidity of the lotion was measured as 6.61 with 6.731 cp of viscosity. The lotion adhesiveness test showed 0.21 seconds and the lotion was shown to be oil-in-water. Application of the lotion on incision wounds made in the abdomen of rats showed blood coagulation on the first day and onset of wound reparation process on the second day. Wound observation by the third day showed that the inflicted area had undergone a near-complete reparation. The study also showed that on the fifth day of reparation Sargassum sp. extract lotion treatment group showed fibroblast formation, as opposed to the non-extract lotion treatment group. Previous study use alginates as wound dressing material, but in this study alginate used as active ingredient in wound healing treatment. Therefore, this study concludes that sodium alginate in Sargassum sp. seaweed has a potential application in the field of medicine.
Genetic Difference between Two Phenotypically Similar Members of Asteraceae By the Use of Intergenic Spacer atpB – rbcL Agus Hery Susanto; Murni Dwiati
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.22137

Abstract

Two Asteraceae species, i.e. Synedrella nodiflora (L.) Gaertn and Eleutheranthea ruderalis (Swartz) Sch.-Bpi. are phenotypically similar with each other, although some differences in morphological and anatomical traits are apparently observable. Molecular comparison using particular marker is required to support a phenotype-based study that previously reported. Chloroplast DNA marker, . atpB – rbcL IGS, was used to identify genetic difference between both species. Six samples of the respective species were collected randomly from some places in Banyumas Regency, Central Java, Indonesia. Amplification of the marker was performed employing a pair of universal primers. Sequence alignment on the PCR products showed that no difference in atpB – rbcL IGS sequences, either within S. nodiflora or E. ruderalis samples was observed. On the other hands, several deletions and base substitution in both S. nodiflora and E. ruderalis were detected when alignment was made between both species. This result suggests that they reveal a convincing genetic difference. Inspite of no direct correlation between this genetic and some visible phenotypic differences, this finding provides preliminary scientific background on the phenotypic traits of both species, which are often difficult to find at a rapid observation.
Diversity and Conservation Knowledge of Chondrichthyans in Northern Coastal Areas of Central Java Ning Setiati; Endah Peniati; Partaya Partaya
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.15341

Abstract

Northern coastal areas of Central Java potentially produces sharks and rays (Chondrichthyes) for dishes. However, lack of community understanding of protected species catchment affects the Chondrichthyans conservation management. This study aimed to identify of total Chondrichthyans that were captured by fisherman correlated to the conservation knowledge and action in northern coastal areas of Central Java. This research was conducted by using interview, and direct observation. Samples were collected in fish capture household places in Tegal, Pemalang, Pekalongan, Semarang, Pati, and identified in Animal Taxonomy Laboratory of Biology Department, Universitas Negeri Semarang. Data were analyzed using descriptive qualitative based on the morphology characteristic. This research found that every season, at least 1,106 individuals from 23 species of Rajiformis and Lamniformis (Chondrichthyes) orders was captured. This condition was aggravated with lack of access to information and low fisherman’ awareness of protected species. It was also found that shark and ray trading generates value chain in culinary business. It increases fish consumption demand that only can be provided by fish catchment from the ocean. The implication of species catchment is declining population of Chondrichthyes in Central Java north areas. Based on the research, a high demand and lack of access to information correlated with declining community awareness on conservation actions. this research adds more information as an effort to conserve shark and ray species based on problems faced by fishermen. The information is also able to be applied as a foundation to develop community based conservation action.
PERBAIKAN PERTUMBUHAN DAN HASIL STEVIA (Stevia rebaudiana BERTONI M) MELALUI APLIKASI Trichoderma sp. Haryuni -
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 2 (2013): September 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i2.2746

Abstract

AbstrakTujuan penelitian ini adalah menguji perbaikan pertumbuhan dan hasil stevia (Stevia rebaudiana Bertoni M) melalui penggunaan  Trichoderma sp.  Perbanyakan  Trichoderma sp. dilakukan di laboratorium Balai Proteksi Perkebunan di Salatiga Jawa Tengah. Penelitian dirancang menggunakan rancangan factorial dengan dua faktor. Faktor pertama adalah inokulasi Trichoderma sp (To = tanpa  Trichoderma sp. T1 = menggunakan Trichoderma sp. 100 g.  Faktor kedua adalah variasi aplikasi perlakuan yaitu: 1). S0 =  tanpa perlakuan, 2). S1 = 10 hari sebelum tanam, 3). S3 = tanama dan  4). S3 = 10  hari setelah tanam. Tiap perlakuan diulang tiga ulangan, tiap ulangan terdiri dari 16 tanaman. Hasil penelitian menunjukkan  bahwa inokulasi Trichoderma sp. dan aplikasi perlakuan  S3 = 10 hari setelah tanam dapat meningkatkan pertumbuhan dan hasil dari  tanaman tevia. AbstractThe object of this research was examine to repair of growth and yield of stevia (Stevia rebaudiana Bertoni M) by Trichoderma sp. application.  Reproduction of Trichoderma sp. performed in the laboratory center of Protection plantation Central of Java at Salatiga.The experiment was arranged in a completely randomized completely factorial design, consisted of two factors. The first factor was Trichoderma sp inoculation which were To = without Trichoderma sp. and T1 = Trichoderma sp. 100 g. The second factor was variation  of plant application treatment, which were 1). S0 = without treatment, 2). S1 = 10 days before of planting , 3). S3 = planting  and 4). S3 = 10 days after planting. Each treatment was repeated three times and each replicate consisted of 16 plants. The result showed that that the inoculation of Trichoderma sp.  and application of treatmen 4 is S3= 10 days after planting increases plant growth and yields of stevia.
Colour and Morphometric Variation of Donacid Bivalves from Nepa Beach, Madura Island, Indonesia Reni Ambarwati; Ulfi Faizah
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11267

Abstract

Donacid bivalves vary in colour, pattern, and the shape of the shells. A population of donacid bivalves had been found in the northern shore of Madura Island, Indonesia. This study aimed to identify the donacids found in northern shore of Madura Island as well as to describe their variation on morphometric and morphological characters. Colour and pattern of 215 shells were observed and carefully photographed. Morphometric of the shells including the shell height, shell length, shell width, dorsal length, and umbo-margin length were measured and then their patterns were analyzed using regression analysis. The results revealed that there were two species of donacid bivalves in Nepa Beach, namely Donax cuneata and Donax faba with different morphological characteristics, including twelve different patterns and colours of the shells. These variations were described completely in this paper. This study contributes to the research on bivalve taxonomy, and is useful for the identification of donacid bivalves based on morphological characteristics.
The Potential Fruit Crop of Cibodas Botanical Garden Suluh Normasiwi; Muhammad Imam Surya
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5235

Abstract

As an institute for ex situ plant conservation of high mountains, Cibodas Botanical Garden (CBG), has more than 1652 species and 8140 specimens of plant collections. An inventory of potential fruit crop in CBG which will support the conservation program had never been done before. The aim of this activity is to determine its potential collections as fruit crop. Descriptive analysis was used to analyze all the data achieved from registration unit and catalogue of (CBG). The results showed that 422 numbers of collections from 31 family, 56 genus and 114 species have high potential as a fruit crop. Moreover, Cibodas Botanical Garden has 74% collection of indigenous fruit (included 85 species) and 61% collection of underutilize fruit (included 68 species) from the total number of fruit plant collections. Most of potential plant collections are able to be developed as an edible fruit crop in Indonesia in order to enhance local food security through diversification of fruit crop.How to CiteNormasiwi, S., Surya, M. I. (2016). The Potential Fruit Crop of Cibodas Botanical Garden. Biosaintifika: Journal of Biology Biology Education, 8(2), 206-213.
Total Phenol Content of Avicennia marina Leaf and Its Relationship to the Environmental Quality Endah Dwi Hastuti; Munifatul Izzati; Sri Darmanti
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23957

Abstract

Environmental dynamic in the coastal area is suggested to affect the metabolite concentration in mangrove plants. This research aimed to study the concentration of total phenol in A. marina leaf and to analyze the effect of environmental parameters on total phenol content dynamics. Environmental parameters studied in this research was temperature, pH, DO, and salinity, as well as N, P, and C sediment content. Data analysis was carried out through multiple regression of natural logarithm transformed data. Laboratory analysis resulted the value of total phenol content in A. marina leaf ranging from 0.88 – 1.62% with the average concentration of 1.28 ± 0.28%. Regression analysis resulted the significant effect of temperature, DO, and sediment content P and C expressed in the formula: ln(TP) = 31.229 – 7.224ln(T) – 0.067ln(DO) – 1.054ln(P) – 1.241ln(C). The research implicated that the increasing value of those factors was approaching the suitable condition for A. marina. Thus, instead of increasing the phenol concentration, the parameters negatively effect the secondary metabolite. The result showed that increasing temperature, DO, and content of P and C reduced the stress in A. marina and reduce total phenol content. This suggests that low temperature, DO, P and C concentration provides more potential of phenolic products from A. marina. 

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