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Inhibitory Activity of Andrographolide and Andrograpanin on the Rate of PGH2 Formation Sumiwi, Sri A.; Halimah, Eli; Saptarini, Nyi M.; Levita, Jutti; Nawawi, Asari; Mutalib, Abdul; Ibrahim, Slamet
Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

Cyclooxygenase (COX) or prostaglandin H2 synthase (PGHS) catalyzes the conversion of arachidonic acid into prostaglandins. Nonsteroidal anti-inflammatory drugs (NSAIDs) work by inhibiting both COX-1 and COX-2 isoforms, thus disturbing this reaction. In Indonesia, Andrographis paniculata (local name: sambiloto), is empirically used to reduce inflammation by consuming the herb tea of this plant. This work studied the inhibitory activity of andrographolide and andrograpanin, diterpenoids of the plant, on the rate of prostaglandin formation. Previous works have proven that andrographolide inhibited PGE2 production in LPS-induced human fibroblast cells. This study was performed by measuring the absorbance of TMPD (tetramethyl-p-phenyldiamine) oxidized by andrographolide and andrograpanin. Acetosal was used as a control drug. The rate of PGH2 formations on either COX-1 or COX- 2 was affected by andrographolide and andrograpanin. Andrographolide and andrograpanin interact longer with COX-1 than COX-2. Andrographolide shows weak inhibition on the rate of PGH2 formation, whilst andrograpanin might be further developed for potential antiinflammatory drugs.Keywords: Andrographis paniculata, anti-inflammatory, COX, cyclooxygenase, prostaglandin

Diabetes Patients Perceptions on Drugs Consultation Program by Pharmacists Sumaryono, Budi; Moektiwardoyo, Moelyono; Yulianto, Ari
Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

Drugs consultation by pharmacist is an important service to improve the quality of medication use in patients with type 2 diabetes mellitus (T2DM). Perception of patients regarding this service is very useful to boost pharmacists confidence to conduct such service. The purpose of this study is to provide an overview regarding interests and categories of drugs consultation needed by T2DM patients and to determine the effect of quality of drugs consultation on patients interest to participate in such program. Survey and interviews were conducted with T2DM patients in outpatient clinic, Dr. M. Salamun Air Force Hospital, Bandung during April-June 2013. One hundred patients were recruited for this study. Most of the patients consumed combination of oral antidiabetic and non-antidiabetic medication (71 %) and were given polypharmacy (45 %). The results showed that the information regarding drugs interactions, adverse drugs reaction (ADR), and drugs dosage were among the aspects that should be included in drugs consultation service by pharmacists. The quality of drug information service was associated with increased interest to participate in such program. These aspects should be considered in the implementation of drugs consultation program by pharmacists.Keywords: drugs consultation, T2DM, pharmacists

Physicochemical Properties of Phosphate Pregelatinized Musa balbisiana Starch as Pharmaceutical Excipient Anggraini, Deni; Lukman, Anita; Teruna, Hilwan Y.
Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

Starch is among the important pharmaceutical excipient, which is particularly used as filler in the tablet formulation and suspending agent. Starch from natural source has a potential to be developed as pharmaceutical excipient with comparable characteristics, including starch from Musa balbisiana (M. balbisiana). This study aimed to synthesize and evaluate physicochemical properties of phosphate pregelatinized M. balbisiana starch. We isolated the starch from M. balbisiana and performed pregelatinization. Since pregelatinized starch still could experience retrogradation which cause syneresis, chemical modification of pre-gelatinized starch was conducted using 5% of sodium tripolyphosphate at pH of 9-10. Physicochemical properties of phosphate pregelatinized starch were then investigated. It included the assessment of its organoleptic, pH, water content, particle size distribution, angle of repose, swelling power, amylose content, adsorbs isotherm, and particle analysis using Scanning Electron Microscope (SEM). Phosphate-pregelatinized M. balbisiana starch had low swelling power, better flow properties and viscosity. It can be used particularly in the formulation of slow released tablets and suspending agents in suspension formula.Keywords: pregelatinized starch, Musa balbisiana, psychochemical properties, excipients

Secondary Metabolites and Antioxidant Activity of Methanol Extract of Castanopsis costata Leaves Alkandahri, Maulana Y.; Nisrihadi, Leo; Salim, Emil
Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

Castanopsis costata (C.costata) leaves were empirically used by people in North Sumatera, Indonesia, as an antioxidant dietary supplement. However, its pharmacological effect has not been scientifically explored. The purpose of this study was to identify the secondary metabolites and antioxidant activity of C. costata. The leaves were cold extracted with methanol. The secondary metabolites were determined using thin layer chromatography. Its antioxidant activity was investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The results showed that methanol extract of C. costata leaves consisted of alkaloids, flavonoids, glycosides, anthraquinone glycosides, tannins and triterpenoids. The methanol extract of C. costata leaves showed comparable antioxidant activity with vitamin C, IC50 35.56 Î¼g/ml and 14.17 Î¼g/ml, respectively.Keywords: Castanopsis costata leaves, methanol extract, antioxidant, DPPH, vitamin C.

Efficacy and Side Effects of Deferasirox and Deferiprone for Thalasemia Major in Children Rindarwati, Asti Y.; Diantini, Ajeng; Lestari, Keri
Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

Thalassemia major (TM) is an inherited disease caused by defective or absent of hemoglobin chain synthesis. Regular chelation therapy is necessary to reduce excess iron in several organs of TM patients. The most commonly used chelating agents are deferasirox and deferiprone. However, information regarding their effectiveness and side effects in Indonesian children population with TM were limited. This study was conducted to assess the effectiveness and side effects of deferasirox and deferiprone in pediatric patients with TM. This was an observational study with prospective analysis which was conducted during April-August 2015. We included pediatric patients with TM who visited a hospital in Bandung, Indonesia, using consecutive sampling method. Thirty two subjects were divided into two groups, i.e., deferasirox and deferiprone group. Review of medical records and interview were performed for each participants. Effectiveness was defined as reduction in ferritin level. Side effects were assessed using Naranjo scale. Data were analyzed using Mann-Whitney test, Wiloxon test and Chi square test. P value < 0.05 defined statistical significance. We found that deferasirox was more effective than deferiprone for the treatment of TM in pediatric patiens, with less side effects. The use of deferasirox as iron chelating agent is recommended for patients with TM.Keywords: deferasirox, deferiprone, ferritin, thalassemia major

Inhibitory Activity of Andrographolide and Andrograpanin on the Rate of PGH2 Formation Sri A. Sumiwi; Eli Halimah; Nyi M. Saptarini; Jutti Levita; As'ari Nawawi; Abdul Mutalib; Slamet Ibrahim
Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

Cyclooxygenase (COX) or prostaglandin H2 synthase (PGHS) catalyzes the conversion of arachidonic acid into prostaglandins. Nonsteroidal anti-inflammatory drugs (NSAIDs) work by inhibiting both COX-1 and COX-2 isoforms, thus disturbing this reaction. In Indonesia, Andrographis paniculata (local name: sambiloto), is empirically used to reduce inflammation by consuming the herb tea of this plant. This work studied the inhibitory activity of andrographolide and andrograpanin, diterpenoids of the plant, on the rate of prostaglandin formation. Previous works have proven that andrographolide inhibited PGE2 production in LPS-induced human fibroblast cells. This study was performed by measuring the absorbance of TMPD (tetramethyl-p-phenyldiamine) oxidized by andrographolide and andrograpanin. Acetosal was used as a control drug. The rate of PGH2 formations on either COX-1 or COX- 2 was affected by andrographolide and andrograpanin. Andrographolide and andrograpanin interact longer with COX-1 than COX-2. Andrographolide shows weak inhibition on the rate of PGH2 formation, whilst andrograpanin might be further developed for potential antiinflammatory drugs.Keywords: Andrographis paniculata, anti-inflammatory, COX, cyclooxygenase, prostaglandin

Diabetes Patients Perceptions on Drugs Consultation Program by Pharmacists Budi Sumaryono; Moelyono Moektiwardoyo; Ari Yulianto
Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

Drugs consultation by pharmacist is an important service to improve the quality of medication use in patients with type 2 diabetes mellitus (T2DM). Perception of patients regarding this service is very useful to boost pharmacists confidence to conduct such service. The purpose of this study is to provide an overview regarding interests and categories of drugs consultation needed by T2DM patients and to determine the effect of quality of drugs consultation on patients interest to participate in such program. Survey and interviews were conducted with T2DM patients in outpatient clinic, Dr. M. Salamun Air Force Hospital, Bandung during April-June 2013. One hundred patients were recruited for this study. Most of the patients consumed combination of oral antidiabetic and non-antidiabetic medication (71 %) and were given polypharmacy (45 %). The results showed that the information regarding drugs interactions, adverse drugs reaction (ADR), and drugs dosage were among the aspects that should be included in drugs consultation service by pharmacists. The quality of drug information service was associated with increased interest to participate in such program. These aspects should be considered in the implementation of drugs consultation program by pharmacists.Keywords: drugs consultation, T2DM, pharmacists

Physicochemical Properties of Phosphate Pregelatinized Musa balbisiana Starch as Pharmaceutical Excipient Deni Anggraini; Anita Lukman; Hilwan Y. Teruna
Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

Starch is among the important pharmaceutical excipient, which is particularly used as filler in the tablet formulation and suspending agent. Starch from natural source has a potential to be developed as pharmaceutical excipient with comparable characteristics, including starch from Musa balbisiana (M. balbisiana). This study aimed to synthesize and evaluate physicochemical properties of phosphate pregelatinized M. balbisiana starch. We isolated the starch from M. balbisiana and performed pregelatinization. Since pregelatinized starch still could experience retrogradation which cause syneresis, chemical modification of pre-gelatinized starch was conducted using 5% of sodium tripolyphosphate at pH of 9-10. Physicochemical properties of phosphate pregelatinized starch were then investigated. It included the assessment of its organoleptic, pH, water content, particle size distribution, angle of repose, swelling power, amylose content, adsorbs isotherm, and particle analysis using Scanning Electron Microscope (SEM). Phosphate-pregelatinized M. balbisiana starch had low swelling power, better flow properties and viscosity. It can be used particularly in the formulation of slow released tablets and suspending agents in suspension formula.Keywords: pregelatinized starch, Musa balbisiana, psychochemical properties, excipients

Secondary Metabolites and Antioxidant Activity of Methanol Extract of Castanopsis costata Leaves Maulana Y. Alkandahri; Leo Nisrihadi; Emil Salim
Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

Castanopsis costata (C.costata) leaves were empirically used by people in North Sumatera, Indonesia, as an antioxidant dietary supplement. However, its pharmacological effect has not been scientifically explored. The purpose of this study was to identify the secondary metabolites and antioxidant activity of C. costata. The leaves were cold extracted with methanol. The secondary metabolites were determined using thin layer chromatography. Its antioxidant activity was investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The results showed that methanol extract of C. costata leaves consisted of alkaloids, flavonoids, glycosides, anthraquinone glycosides, tannins and triterpenoids. The methanol extract of C. costata leaves showed comparable antioxidant activity with vitamin C, IC50 35.56 μg/ml and 14.17 μg/ml, respectively.Keywords: Castanopsis costata leaves, methanol extract, antioxidant, DPPH, vitamin C.

Efficacy and Side Effects of Deferasirox and Deferiprone for Thalasemia Major in Children Asti Y. Rindarwati; Ajeng Diantini; Keri Lestari
Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

Thalassemia major (TM) is an inherited disease caused by defective or absent of hemoglobin chain synthesis. Regular chelation therapy is necessary to reduce excess iron in several organs of TM patients. The most commonly used chelating agents are deferasirox and deferiprone. However, information regarding their effectiveness and side effects in Indonesian children population with TM were limited. This study was conducted to assess the effectiveness and side effects of deferasirox and deferiprone in pediatric patients with TM. This was an observational study with prospective analysis which was conducted during April-August 2015. We included pediatric patients with TM who visited a hospital in Bandung, Indonesia, using consecutive sampling method. Thirty two subjects were divided into two groups, i.e., deferasirox and deferiprone group. Review of medical records and interview were performed for each participants. Effectiveness was defined as reduction in ferritin level. Side effects were assessed using Naranjo scale. Data were analyzed using Mann-Whitney test, Wiloxon test and Chi square test. P value < 0.05 defined statistical significance. We found that deferasirox was more effective than deferiprone for the treatment of TM in pediatric patiens, with less side effects. The use of deferasirox as iron chelating agent is recommended for patients with TM.Keywords: deferasirox, deferiprone, ferritin, thalassemia major

Home Page

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Editorial Team

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Reviewer

Google Scholar

Contact Name
Widya Norma Insani, M.Sc., Apt.

Contact Email
Widya Norma Insani, M.Sc., Apt.

Phone
-

Journal Mail Official
pcpreditorialteam@gmail.com

Editorial Address
-

Location
Kota bandung,

Jawa barat

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Widya Norma Insani, M.Sc., Apt.

Contact Email Widya Norma Insani, M.Sc., Apt.

Phone -

Journal Mail Official pcpreditorialteam@gmail.com

Editorial Address -

Location Kota bandung, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Widya Norma Insani, M.Sc., Apt.

Contact Email
Widya Norma Insani, M.Sc., Apt.

Phone
-

Journal Mail Official
pcpreditorialteam@gmail.com

Editorial Address
-

Location
Kota bandung,

Jawa barat

INDONESIA