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The determination of quercetin in Plectranthus scutellarioides(L.) R.Br. leaves extract and its In SilicoStudy on Histamine H4 Receptor Moektiwardoyo, Moelyono; Levita, Jutti; Sidiq, Syafrudin Purnama; Ahmad, Khoziah; Mustarichie, Resmi; Subarnas, Anas; ., Supriyatna
Indonesian Journal of Pharmacy Vol 22 No 3, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.578 KB) | DOI: 10.14499/indonesianjpharm0iss0pp191-196

Abstract

Plectranthus  scutellarioides  (L.)  R.Br.,  or  jawer  kotok,  Family  Lamiaceae, grows  widely  in  Indonesia,  and  has  a  long  history  of  therapeutic  usage  in Indonesian traditional jamuto cure various diseases. The brownish purple leaves of  Plecranthus  contain  alkaloids,  saponin,  flavonoids,  tannin,  volatile  oils,  and quercetin  which  has  been  proven  to  exert  antiinflammatory  activity.  In  this research,  a  determination  of  quercetin  in  Plecranthus  leaves  extract  was performed and followed by a study of its interaction with histamine H4 receptor to  understand its  anti-inflammatory  activity.  The  dry  leaves  were  macerated by using  a  mixture  of  methanol  and  water  (1:1)  for  48  hours  and  the  solvent  was evaporated  at  low  temperature  (40-50oC).  Analysis  of  quercetin  in  the  extract was performed by using reversed-phase HPLC method LC-10AT VP (Shimadzu), Atlantis  Hilicsilica  C18  (Waters®)  150  mm  x  4.6  mm,  5  µm  as  stationary  phase and  a  mixture  of  acetonitrile,  phosphoric  acid,  and methanol  (40:50:10),  flow rate 0.8 mL/minute.  In silicostudy of quercetin with histamine H4 receptor was performed by using AutoDock Tools 3.0.5. Histamine H4 receptor (H4R) belongs to  G  protein-coupled  receptors  which  is  involved  in arthritis,  asthma,  and inflammations.  The  3D  structure  model  of  H4R  was  built  by  using  MODELLER 9v7.  Quercetin  contained  in  Plecranthus  leaves  extract  was  0.05  %.  This compound interacted with H4R viahydrogen bond formation with Lys158 (2.006 Å)  and  Glu182  (2.048  Å),  and  van  der  Waals  interaction  with  Trp90,  Leu91, Asp94, Tyr95, Phe168, Thr178, Ser179, Tyr319, Phe344, and Tyr340, therefore Plecranthus  leaves  extract  might  have  a  chance  to  be  used  as  histamine  H4 receptor inhibitor.Key  words  :   histamine  H4  receptor,  in  silico  study,  Plecranthus  leaves,  Plectranthus scutellarioides, quercetin
Analisis Mikroba, Timbal, Kadmium, dan Raksa pada Es Balok dan Bahan Pembuatannya Andriany, Dinny; Levita, Jutti; Warya, Sohadi; Maelaningsih, Firdha S
Indonesian Journal of Pharmaceutical Science and Technology Vol 1, No 2 (2014)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.672 KB) | DOI: 10.15416/ijpst.v1i2.7511

Abstract

Telah dilakukan penetapan kadar timbal, kadmium, dan raksa pada sampel es balok dan sumber air bahan baku pembuatan es balok yang berasal dari salah satu pabrik di Bandung menggunakan metode spektrofotometri serapan atom tanpa nyala. Penetapan kadar dilakukan berdasarkan persyaratan air minum yang terdapat dalam Permenkes RI No. 492/MENKES/PER/VI/2010. Hasil penelitian menunjukkan bahwa cemaran logam terbanyak yaitu es balok dari depot. Kadar timbal tertinggi dalam es balok berasal dari depot adalah 15,5 ppb dan kadar kadmium tertinggi adalah 11 ppb, sedangkan kadar timbal tertinggi pada air bahan baku adalah 4,32  ppb dan 5,77 ppb pada es balok, dan kadar kadmium tertinggi pada air bahan baku adalah 1,85 ppb, dan pada es balok sebesar 0,88 ppb. Kadar raksa tertinggi untuk air bahan baku dan es balok adalah 0,06 ppb. Dari hasil tersebut diketahui bahwa ketiga logam yang diperiksa tidak melebihi batas yang ditetapkan yaitu 10 ppb untuk timbal, 3 ppb untuk kadmium, dan 1 ppb untuk raksa. Kata kunci: Es balok, kadmium, raksa, timbal
Formulasi dan Uji Stabilitas Tetes Mata Sulfasetamida Abdassah, Marline; Noviardani, Tenri; Levita, Jutti; Suherman, Shelvy E
Indonesian Journal of Pharmaceutical Science and Technology Vol 2, No 1 (2015)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.024 KB) | DOI: 10.15416/ijpst.v2i1.7808

Abstract

 Tetes mata sulfasetamidayang mengandung natrium sulfasetamida 10%, 15%, dan 30% telah dibuat dan disterilkan. Metode sterilisasi yang digunakan adalah uap air mengalir 98-100 oC, penyaring bakteri, dan autoklaf 120-121 oC selama 15 menit. Tujuan penelitian ini adalah mendapatkan sediaan yang paling stabil selama penyimpanan 28 hari. Semua sediaan diamati kejernihan, pH dan konsentrasi natrium sulfasetamida.Semua sediaan mengalami kenaikan pH. Kekeruhan terjadi pada sediaan yang disterilkan dengan autoklaf. Sediaan paling stabil adalah tetes mata yang mengandung natrium sulfasetamid 10% yang disterilisasi dengan penyaring bakteri.
Peningkatan Permeasi Mikroemulsi Ketoprofen Gozali, Dolih; Warhead, Fara; Levita, Jutti; Khoirunisa, Anis
Indonesian Journal of Pharmaceutical Science and Technology Vol 2, No 2 (2015)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.418 KB) | DOI: 10.15416/ijpst.v2i2.7811

Abstract

Penggunaan obat yang sukar larut air tidak efisien apabila kadar penetrasi obat ke dalam tubuh sangat kecil. Mikroemulsi ialah satu sistem dispersi yang dapat meningkatkan kelarutan obat. Pada penelitian ini dilakukan formulasi mikroemulsi ketoprofen menggunakan zat peningkat penetrasi mentol (0%, 1%, 3%, 5%). Asam oleat digunakan sebagai fase minyak, tween 80 sebagai komponen surfaktan, dan propilen glikol sebagai kosurfaktan. Evaluasi sediaan mikroemulsi dilakukan dengan uji permeasi mikroemulsi ketoprofen secara in vitro menggunakan sel difusi Franz terhadap membran kulit ular dengan medium dapar fosfat pH 7,4 selama 90 menit. Formula F4 (mengandung mentol 5%) menunjukkan permeasi tertinggi setelah 90 menit (16,21 ppm) dibandingkan dengan F1 (tidak mengandung mentol). Keempat formula memiliki kestabilan yang baik selama pengamatan. Dapat disimpulkan bahwa mentol meningkatkan permeasi ketoprofen. Kata kunci: Ketoprofen, mentol, mikroemulsi, uji permeasi
Stabilitas Sampel SOD-Eritrosit dan GPx-Blood dalam Masa Penyimpanan Tujuh Hari Fattah, Miswar; Anggraeni, Sra R.; Alfian, Sofa D.; Levita, Jutti; Diantini, Ajeng
Indonesian Journal of Clinical Pharmacy Vol 1, No 4 (2012)
Publisher : Indonesian Journal of Clinical Pharmacy

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Abstract

Telah dilakukan penelitian mengenai aktivitas SOD eritrosit pada hari ke-0, 1, 3, 5, 7 dengan sentrifugasipada suhu ruangan (22,5 0C) dan pada suhu penyimpanan -80 0C, aktivitas SOD pada hari ke-0 dengan sentrifugasi pada suhu 4 0C, dan aktivitas SOD dengan inkubasi sampel whole blood selama satu hari pada suhu 2–8 0C serta aktivitas GPx hari ke-0, 1, 3, 5, 7 pada suhu penyimpanan 2–8 0C. Penelitian ini menggunakan reagen dari Randox Laboratories yang diukur pada panjang gelombang 505 nm untuk SOD dan 340 nm untuk GPx menggunakan alat Hitachi 917 dari Boehringer Mannheim. Data yang dianalisis menggunakan metode t-test menunjukkan bahwa aktivitas SOD pada hari ke-0, 1, 3, 5, dan 7 dengan sentrifugasi pada suhu ruangan tidak menunjukkan perbedaan yang signifikan, sedangkan pada hari ke-0 dengan sentrifugasi pada suhu 4 0C dan inkubasi sampel whole blood selama 1 hari pada suhu 2–8 0C berbeda secara signifikan. Aktivitas GPx pada hari ke-3 tidak berbeda secara signifikan, sementara aktivitas pada hari ke-0, 1, 5 dan 7 terdapat perbedaan yang signifikan.Kata kunci: Stabilitas enzim, Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx) Stability of Seven Days Sample Storage of Erythrocyte’s SOD and Blood’s GPxAbstractThe research was about SOD erythrocyte activities at day 0, 1, 3, 5, and 7 which centrifuged at room temperature (22.5 0C) and storage temperature (-80 0C), SOD activities at day-0 which centrifuged at 4 0C, SOD whole blood activities with one day incubated at 2-8 0C and GPx activities at day 0, 1, 3, 5, and 7 with 2–8 0C storage temperature. Laboratory analysis were performed by using reagent from Randox Laboratories, and Hitachi 917 analyzer from Boehringer Mannheim. SOD activities were measured at 505 nm absorbance meanwhile 340 nm absorbance is used to measure GPx. Data was analyzed by using t-test method and showed that SOD activities at day 0, 1, 3, 5, and 7 with room temperature centrifuged had no significant differences. Significant differences are found at day-0 with centrifuged at 4 0C and one day incubated whole blood at 2–8 0C. GPx activities at day- 3 had no significant differences. Significant differences are found at day-0,1, 5 and 7 after storage.Key words: Enzyme stability, Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx)
Inhibitory Activity of Andrographolide and Andrograpanin on the Rate of PGH2 Formation Sumiwi, Sri A.; Halimah, Eli; Saptarini, Nyi M.; Levita, Jutti; Nawawi, Asari; Mutalib, Abdul; Ibrahim, Slamet
Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.747 KB) | DOI: 10.15416/pcpr.v1i3.15246

Abstract

Cyclooxygenase (COX) or prostaglandin H2 synthase (PGHS) catalyzes the conversion of arachidonic acid into prostaglandins. Nonsteroidal anti-inflammatory drugs (NSAIDs) work by inhibiting both COX-1 and COX-2 isoforms, thus disturbing this reaction. In Indonesia, Andrographis paniculata (local name: sambiloto), is empirically used to reduce inflammation by consuming the herb tea of this plant. This work studied the inhibitory activity of andrographolide and andrograpanin, diterpenoids of the plant, on the rate of prostaglandin formation. Previous works have proven that andrographolide inhibited PGE2 production in LPS-induced human fibroblast cells. This study was performed by measuring the absorbance of TMPD (tetramethyl-p-phenyldiamine) oxidized by andrographolide and andrograpanin. Acetosal was used as a control drug. The rate of PGH2 formations on either COX-1 or COX- 2 was affected by andrographolide and andrograpanin. Andrographolide and andrograpanin interact longer with COX-1 than COX-2. Andrographolide shows weak inhibition on the rate of PGH2 formation, whilst andrograpanin might be further developed for potential antiinflammatory drugs.Keywords: Andrographis paniculata, anti-inflammatory, COX, cyclooxygenase, prostaglandin
Prediction of Binding Mode of Secondary Metabolites in Apium graveolens to Bcl-2 Shan, Kee P.; Saptarini, Nyi M.; Levita, Jutti
Pharmacology and Clinical Pharmacy Research Vol 2, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.792 KB) | DOI: 10.15416/pcpr.v2i2.15209

Abstract

Developing new cytotoxic agent which has minimal effect against normal cell is requiredto reduce the side effects obtained from the existing chemotherapy agents. Celery (Apiumgraveolens) showed several pharmacology activities, including anti-cancer. This study wasconducted to predict and visualize the binding mode of ten secondary metabolites in A. graveolens,i.e., apigenin, caffeic acid, kaempferol, limonene, shikimic acid, chlorogenic acid,ascorbic acid, quercetin, eugenol and ferulic acid against Bcl-2. Navitoclax was employed asreference. Molecular dokcing simulation was performed using AutoDoc Vina v.1.5.6. Theresults showed that the interaction enery (Ei) ranged from -4.20 to -6.01 kcal/mol, whereasinhibition constant (Ki) were 40.15 to 842.29 μM. Kaempferol showed the best affinity toBcl-2 (Ei=-6.01 kcal/mol; Ki=24,21 μM). Hydrogen bonds were bond between kampferoland Lys20, Ser102, and Arg103, amino acid residues in Bcl-2.In conclusion,Keywords: anti-cancer, Apium graveolens, Bcl-2, navitoclax
Zingiber officinale var. Rubrum Reduces the Rate of Prostaglandin Production Fikri, Fauzan; Saptarini, Nyi M.; Levita, Jutti; Nawawi, Asari; Mutalib, Abdul; Ibrahim, Slamet
Pharmacology and Clinical Pharmacy Research Vol 1, No 1
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (594.225 KB) | DOI: 10.15416/pcpr.v1i1.15200

Abstract

In Indonesia, red ginger (Zingiber officinale var. Rubrum) is usually used as topical pain reducer by directly applying the fresh rhizome. The aim of this research was to provide information regarding the pharmacological activity of Z. officinale var. Rubrum rhizome infusion on the rate of prostaglandin production. The Z. officinale var. Rubrum rhizome used in this research was purchased from Research Institute for Spices and Medicinal Plants (Balittro) Manoko Lembang, West Java, Indonesia. This research was conducted by applying TMPD (N,N,N’,N’-tetramethyl-p-phenylendiamine) as the reagent. COX-1 and COX-2 enzyme inhibitory activity can be seen from TMPD chromogenic changes that occur during PGG2 reduction to PGH2. Phytochemical screening showed that flavonoid, quinone, and monoterpenoid/sesquiterpenoid were detected in both dried rhizomes and the water extract. Three spots were detected on thin-layer chromatography system which employing chloroform-methanol (5:5) as the eluent. The rate of prostaglandin formations either by Z. officinale var. Rubrum rhizome infusion or acetylsalicylic acid on COX-1 is slower (at 25th minutes) rather than COX-2 (5th minutes). We concluded that the rhizome of Z. officinale var. Rubrum reduces the rate of prostaglandin production. The rhizome of red ginger reduces the rate of prostaglandin production, which is slower in COX-1 than in COX-2. This plant could be further developed as anti-inflammatory drug candidate.Keywords: acetosal, antiinflammation, cyclooxygenase, NSAIDs, red ginger
Predictor of Diabetes: Correlation between Leucine Concentration and Insulin Resistance Wijaya, Chyntia R.; Sukmana, Indriyanti R.; Levita, Jutti
Pharmacology and Clinical Pharmacy Research Vol 2, No 3
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.181 KB) | DOI: 10.15416/pcpr.v2i3.16225

Abstract

Leucine catabolism changes among people with central obesity. This condition can lead to metabolic pathway disorder and increased mTORC-1 activation. Downstream signal of mTORC-1 is p70S6K1, which causes phosphorylation of insulin-1 receptor substrate (IRC-1). This study was performed to evaluate correlation between leucine concentration and insulin resistance (IR). This study was a prospective cross-sectional study, involving two groups; control and obese group. General characteristics and blood sample were taken from each subject. Leucin and Homeostasis Model Assesment (HOMA)-IR, as the marker of insulin resistance, were evaluated. The result indicated a significant positive correlation between leucine concentration and insulin resistance value (R=0.351; P=0.006) in central obese men. The higher leucine concentration, the higher the risk of insulin resistance occurrance. Therefore, leucine can be used as a biomarker for early detection of insulin resistance.Keywords: amino acids, mTORC-1, insulin-1 receptor substrate
MODE IKATAN METABOLIT SEKUNDER DI TANAMAN AKAR KUNING (Arcangelisia flava L.) DENGAN NITRAT OKSIDA SINTASE Kolina, Jennifer; Sumiwi, Sri Adi; Levita, Jutti
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 8, No 1 (2018): Fitofarmaka, Vol.8, No.1, Juni 2018
Publisher : Universitas Pakuan

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Abstract

ABSTRAKInflamasi dapat menginduksi NOS (nitrat oksida sintase), yaitu enzim pengkatalisispembentukan NO (nitrat oksida) berlebih sebagai pro-inflamasi. Akar kuning (Arcangelisia flava. L) banyak digunakan sebagai obat tradisional oleh masyarakat Asia Tenggara, baik sebagai obat luar maupun obat dalam. Senyawa kimia yang terkandung di dalam A. flava antara lain saponin, flavonoid dan tanin.   Akar tanaman ini juga mengandung glikosida dan alkaloid, terutama golongan isokuinolin, yaitu berberin, jatrorizin dan palmatin. Terdapat juga beberapa alkaloid minor seperti kolumbamin, dehidrokoridalmin, homoaromolin dan talifendin, serta diterpen fibraleusin. Fibraurin berpontensi sebagai anti-bakteri, anti-tumor, dan anti-inflamasi. Tujuan penelitian ini adalah untuk menelaah mode ikatan senyawa-senyawa kimia yang terkandung dalam A. flava terhadap enzim NOS dengan metode penambatan molekuler menggunakan perangkat lunak AutoDock. Hasil penelitian menunjukkan bahwa berberin, daidzein, dehidrokorildamin, epikatekhin, hidroksiekdison, jatrorizin, kaempferol, piknarin, kuersetin,  dan  talifendin  dapat  berinteraksi dengan  enzim NOS  pada  kantung  aktif melalui pembentukan ikatan hidrogen dengan residu asam amino  Glu377. Senyawa lainnya berinteraksi dengan residu asam amino tidak spesifik. Tidak ditemukan interaksi dengan Tyr347 pada semua senyawa. Hanya satu senyawa yang tidak membentuk interaksi dengan enzim NOS yaitu fibraurin.Kata kunci: antiinflamasi, AINS, alkaloid, iNOS, metabolit sekunder,   MODE OF BONDING COMPUNDS IN YELLOW ROOTS (Arcangelisia flava L.) WITH NOS ENZUMES F0R ANTIINFLAMMATORY ABSTRACTInflammation can induce NOS (nitric oxide synthase), the enzyme catalyzing the formation of NO (nitric oxide) excess as pro-inflammatory. The yellow root (Arcangelisia flava. L) is widely used as a traditional medicine by the people of Southeast Asia as medicine for external and internal use. Chemical compounds contained in A. flava include saponins, flavonoids and tannins. The root of this plant also contains glycosides and alkaloids, especially groups of isokuinolin, i.e berberine, jatrorizin and palmatin. There are also some minor alkaloids such as kolumbamin, dehidrokoridalmin, homoaromolin and talifendin, as well as fibraleucin. Fibraurin has the potential to be anti-bacterial, anti- tumor, and anti-inflammatory. The purpose of this study was to examine the bonding mode  of chemical  compounds  contained  in  A.  flava  towards  NOS  enzymes  using molecular docking method with AutoDock software. The results showed that berberine, daidzein, dehydrocorilineamine, epicatechin, hydroxyecdison, jatrorizine, kaempferol, piknarin, quercetin, and talifendin interact  with NOS enzymes in the active site by forming hydrogen bonds with Glu377 amino acid residues. Other compounds interact   with non-specific amino acid residues. No interactions with Tyr347 were found in all compounds. Only one compound that does not form an interaction with the NOS enzyme is fibraurin.Keywords : antiinflamation, AINS, alkaloid, iNOS, metabolite
Co-Authors A. Diantini - A. Mutalib - Abdul Mutalib Abdul Mutalib Abdul Mutholib Achmad Al Baihaqi Ade Zuhrotun Agung Karuniawan Ahmad Muhtadi AINAA HAZIRAH SHAMSUL ANUAR AJENG DIANTINI Ajeng Diantini, Ajeng Amalia, Riezki Ami Rahmati Syadiah Anas Subarnas Anas Subarnas Anggraeni, Sra R. Anis Khoirunisa Anis Khoirunisa, Anis Anis Yohana Chaerunisaa Anisa Pebiansyah Anna Yuliana, Anna Ardiansyah, Fahriza As'ari Nawawi As'ari Nawawi As’ari Nawawi Aulifa, Diah L. Batari A. Larasati Bawono, Lidya Cahyo Billy Dwi Saputra Cahya Nova A. Chyntia R. Wijaya Dani Sujana Debby Ustari Diah Lia Aulifa DIANTINI, AJENG - Diina, Tresnafuty Rasyiida Dina Mulyana Syafitri DINA SEMBIRING Dinny Andriany Dinny Andriany, Dinny DOLIH GOZALI Dolih Gozali Driyanti Rahayu Driyanti Rahayu Dwi Lestari Sulistyaningsih Eli Halimah Eli Halimah, Eli Ellin Febrina Ellin Febrina Ellin Febrina Ellin Febrina Enade P. Istyastono Ervita Indriani Fara Warhead Fara Warhead, Fara Fathurrahmi Fathurrahmi Fattah, Miswar Fauzan Fikri Febrina Amelia Saputri Fikri, Fauzan Firdha S Maelaningsih Firdha S Maelaningsih, Firdha S Gofarana Wilar Herawati, Irma E. HILMA AWALIA RAHMAH Ida Musfiroh Ida Musfiroh - Ika WAHYUNI Imam A. Wicaksono Indah Suasani Wahyuni Indah Suasani Wahyuni INDRIANI, ERVITA Indriyanti R. Sukmana Irma Erika Herawati Irma Erika Herawati Irma Erika Herawati Irma Erika Herawati Irma M. Puspitasari, Irma M. Irma Melyani Puspitasari Irma Meylani Puspitasari Iwan J. P. de Esch IYAN RIFKY HIDAYAT Jennifer Kolina Jiranusornkul, Supat Jopi Arpina Kee P. Shan Keri Lestari Khairinisa, Miski Aghnia Khoziah Ahmad, Khoziah KIKI IKRIMA Kolina, Jennifer Marline Abdassah Marline Abdassah Marline Abdassah, Marline Maryam Hasymia Ishmatullah Masripah Masripah Masripah MEGANTARA, SANDRA Megantara, Sandra Meigita Indah Farkhani Ming, Wong Chun Miswar Fattah Mochamad Rizal Moelyono Moektiwardoyo Moelyono Moektiwardoyo, Moelyono Monika Novryanti Monika Novryanti, Monika Muchtaridi Muchtaridi Muhammad Ikhsan Muhammad Ryan Radix Rahardhian Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Mutakin Nawawi, Asari Nela - Simanjuntak Nuraeni Nuraeni Nurdjanah Azinar Nyi M. Saptarini Nyi M. Saptarini Nyi M. Saptarini Nyi Mekar Saptarini Pratidina, Malfa Laila Putri, Refitha Nurul Raden Maya Febriyanti Renny Amelia Renyiska Yula Resha Resmawati Shaleha Resmi Mustarichie Ridwan, Hanna Salwa Riezki Amalia Riezki Amalia Riezki Amalia Riezki Amalia Rini Daud Supu Rini Hendriani Ronny Lesmana Rosdianto, Aziiz M. Rusdianto, Aziiz M. Saeful Amin, Saeful Saptarini, Nyi M. Shaleha, Resha Resmawati Shan, Kee P. Shelvy E Suherman Shelvy E Suherman Shelvy E Suherman, Shelvy E Sitepu, Rini Daud Slamet Ibrahim Slamet Ibrahim Slamet Ibrahim Slamet Ibrahim Slamet Ibrahim Sofa D. Alfian Sofa D. Alfian, Sofa D. Sohadi Warya Sohadi Warya Sra R. Anggraeni Sri A. Sumiwi Sri Adi Sumiwi Sri Adi Sumiwi Sriwidodo Sriwidodo STEFANNY AGNES SALIM Steffi Liem Steffi Liem Sukmana, Indriyanti R. Sumiwi, Sri A. Sumiwi, Sri A. Supriyatna ., Supriyatna Susilawati, Elis Syadiah, Ami Rahmati Syafrudin Purnama Sidiq, Syafrudin Purnama Tarkus Suganda Tenri Noviardani Tenri Noviardani, Tenri TIANA MILANDA Tina Rostinawati Tresnafuty Rasyiida Diina Trixie A. Ulimaz Ulfinana Hafni Vergel Concibido Virda Aziza Wahyuni, Indah S. Wijaya, Chyntia R. Winarni, Rina Wiwiek Indriyati - Wong Chun Ming Yasmiwar Susilawati Yogiyanto Yogiyanto Yudha Prabowo Yuli Andriani Yulin Prisdiany ZAHRA DZAKIRAH ABNAZ Zain, Dichy Nuryadin ZELIKA MEGA RAMADHANIA