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Jurnal Aplikasi Teknologi Pangan
Published by Universitas Diponegoro
ISSN : 20897693     EISSN : 24605921     DOI : -
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Jurnal Aplikasi Teknologi Pangan aims to expose the results of fundamental and applied research in food and its related fields to scholars, students, and food applicants. The journal covers the fields of application of technology on food, i.e. biotechnology, functional food, food process, health, food related field on agribusiness and agro-technology.
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Articles 109 Documents
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Isolasi dan Identifikasi Bakteri Asam Laktat Selulolitik yang Berasal dari Jus Kubis Terfermentasi Cahya Setya Utama; Zuprizal Zuprizal; Chusnul Hanim; Wihandoyo Wihandoyo
Jurnal Aplikasi Teknologi Pangan Vol 7, No 1 (2018): Februari 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1832.599 KB) | DOI: 10.17728/jatp.2155

Abstract

Penelitian bertujuan mengisolasi dan identifikasi jenis bakteri asam laktat (BAL) selulolitik yang berasal dari jus kubis terfermentasi. Tahapan penelitian meliputi isolasi mikrobia dari jus kubis terfermentasi, karakteristik morfologi sel, karakteristik biokimiawi, identifikasi dengan kit api 50 CHL dan pengujian kemampuan mendegradasi selulosa. Penelitian diawali dengan memfermentasi jus kubis selama 6 hari pada kondisi anaerobic fakultatif. Hasil fermentasi diisolasi dengan media de man rogosa and sharpe (MRS) yang ditambahkan CaCO3 1% dan diinkubasi selama 24 jam. Reinokulasi dilakukan sebanyak 5 kali sampai ditemukan kultur murni BAL, kemudian diidentifikasi dengan menggunakan kit analytical profile index (API) 50 CHL. Hasil identifikasi kemudian diuji kemampuannya untuk mendegradasi selulosa pada media MRS yang ditambahkan 1% carboxymethyl cellulosa (CMC) dan 0,1% congored sebagai indikator dan diinkubasi selama 24 jam. Hasil penelitian menunjukkan bahwa strain bakteri yang teridentifikasi adalah Lactobacillus plantarum dan Lactobacillus brevis yang dapat mendegradasi selulosa. Kesimpulan dari penelitian ini adalah ditemukannya 2 jenis bakteri asam laktat dari jus kubis terfermentasi yaitu Lactobacillus plantarum dan Lactobacillus brevis yang mempunyai sifat selulolitik.Isolation and Identification of Lactic Acid Bacteria Cellulolitik Originated from Fermented Cabbage JuiceAbstractThe objective of this study was to isolate and identify the type of lactic acid bacteria (BAL) of cellulolytic originating from fermented cabbage juice. Research was conducted with the following steps: microbial isolation from fermented cabbage juice, cell morphology characteristics, biochemical characteristics, identification with an analytical profile index (API) 50 CHL kit, and cellulose degradability testing. The research was begun by fermenting cabbage juice for 6 days under facultative aerobic condition. The fermentation product was then isolated with de man rogosa and sharpe (MRS) medium which added CaCO3 1% and incubated for 24 hours. Reinoculation was performed 5 times until BAL was found. BAL pure cultures were used to identify lactic acid bacteria strains using an analytical profile index (API) 50 CHL kit. After pure culture was found, it was then tested for the ability to degrade cellulose on MRS media with 1% carboxymethyl cellulose (CMC) and 0.1% congored as indicator and incubated for 24 hours. The results showed that the identified bacterial strains were Lactobacillus plantarum and Lactobacillus brevis which could degrade cellulose. The conclusion of this research was the discovery of two types of lactic acid bacteria from fermented cabbage juice namely Lactobacillus plantarum and Lactobacillus brevis which had cellulolytic properties.
Potensi Yogurt Rosella Probiotik Lactobacillus plantarum IIA-1A5 atau Lactobacillus fermentum B111K dalam Mengasimilasi Kolesterol Asti Yosela Oktaviana; Irma Isnafia Arief; Irmanida Batubara
Jurnal Aplikasi Teknologi Pangan Vol 7, No 3 (2018): Agustus 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.956 KB) | DOI: 10.17728/jatp.2760

Abstract

Yogurt rosella susu kambing merupakan susu kambing fermentasi menggunakan bakteri stater “Lactobacillus bulgaricus dan Streptococcus thermophilus” serta ditambah ekstrak bunga rosella (Hibiscus sabdariffa L). Bakteri Lactobacillus plantarum IIA-1A5 dan Lactobacillus fermentum B111K digunakan sebagai probiotik pada pembuatan minuman yogurt rosella. Tujuan penelitian untuk mengevaluasi karakteristik dan kemampuan yogurt rosella probiotik dengan menggunakan L. plantarum IIA-1A5 dan L. fermentum B111K dalam mengasimilasi kolesterol secara in vitro dengan lama penyimpanan berbeda. Perlakuan yang dilakukan dalam penelitian ini adalah P1 : Yogurt dengan bakteri L. bulgaricus dan S. thermophilus, P2 : Yogurt rosella dengan L. bulgaricus dan S. thermophilus,  P3 : Yogurt rosella dengan bakteri L. bulgaricus, S. thermophilus dan L. plantarum IIA-1A5,  P4 : Yogurt rosella dengan bakteri L. bulgaricus, S. thermophilus dan L. fermentum B111K. Hasil penelitian menunjukkan bahwa bakteri L. plantarum IIA-1A5 dan L. fermentum memberikan pengaruh nyata (P<0.05) terhadap nilai viskositas, aktivitas air, total asam tertitrasi (TAT) selama penyimpanan 15 hari. Penggunaan bakteri L. plantarum IIA-1A5 dan L. fermentum B111K tidak memberikan pengaruh yang nyata (P>0.05) terhadap nilai total bakteri asam laktat (BAL) tetapi lama penyimpanan 15 hari memberikan pengaruh nyata (P<0.05). Karakteristik fisik, kimia dan mikrobiologi yogurt rosella probiotik Lactobacillus plantarum IIA-1A5 dan Lactobacillus fermentum B111K selama penyimpanan 15 hari masih baik dan layak untuk dikonsumsi. Selama penyimpanan 15 hari yogurt rosella probiotik Lactobacillus fermentum B111K (YRPF) memiliki potensi mengasimilasi kolesterol tertinggi dengan kolesterol terasimilasi sebesar 4.59 µg/ml dan persentase kolesterol terasimilasi sebesar 15.7%.Potency of Yogurt Roselle Probiotic Lactobacillus plantarum IIA-1A5 or Lactobacillus fermentum B111K in Assimilating CholesterolAbstractYogurt roselle milk goat is fermented milk goat using starter bacteria "Lactobacillus bulgaricus and Streptococcus thermophilus" probiotic bacteria added with rosella flower extract (Hibiscus sabdariffa L). The Lactobacillus plantarum IIA-1A5 and Lactobacillus fermentum B111K bacteria are used as probiotics in the manufacture of functional beverages of rosella yogurt. The objective of the study was to evaluate the characteristics and abilities of probiotic rosella yogurt by using L. plantarum IIA-1A5 and L. fermentum B111K in assimilating cholesterol by in vitro analysis with different storage times. Treatment conducted in this research was P1: yogurt with L. bulgaricus and S. thermophilus bacteria, P2: Yogurt with L. bulgaricus and S. thermophilus bacteria, P3: Yogurt with bacteria L. bulgaricus, S. thermophilus and L. plantarum IIA-1A5 bacteria, P4: rosella yogurt with stater bacteria and L. fermentum B111K. The results showed that L. plantarum IIA-1A5 and L. fermentum had significant effect (P <0.05) on viscosity, water activity, total titrated acids (TAT) during 15 days storage. The use of L. plantarum IIA-1A5 and L. fermentum B111K bacteria did not give significant effect (P> 0.05) to the total value of lactic acid bacteria (BAL) but 15 days storage time gave significant effect (P <0.05). Physical, chemical and microbiological characteristics of yogurt roselle probiotic L. plantarum IIA-1A5 and yogurt roselle probiotic L. fermentum B111K for 15 days storage are still good and feasible for consumption. During the 15 day storage of probiotic yogurt rosella L. fermentum B111K (YRPF) has the potential to assimilate the highest cholesterol with assimilated cholesterol by 4.59 μg/ml and the assimilated cholesterol percentage of 15.7% 
Kadar Protein, Kadar Lemak dan Solid non Fat Susu Kambing Pasteurisasi pada Penyimpanan Refrigerator Oktavia Rahayu Puspitarini; Merlita Herbani
Jurnal Aplikasi Teknologi Pangan Vol 7, No 1 (2018): Februari 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.562 KB) | DOI: 10.17728/jatp.2162

Abstract

AbstrakPenelitian bertujuan untuk mengetahui pengaruh lama simpan refrigerator terhadap kadar protein, kadar lemak dan solid non fat (SNF) susu kambing pasteurisasi. Penelitian ini telah dilaksanakan di Laboratorium Teknologi Hasil ternak, Fakultas Peternakan, Universitas Islam Malang mulai tanggal 01 Juli – 21 Agustus 2017. Materi yang digunakan adalah susu kambing, gula, alat pasteurisasi sederhana, refrigerator, lactoscan. Metode dalam penelitian ini adalah eksperimen. Parameter yang diamati adalah kadar protein, kadar lemak dan solid non fat (SNF). Pengujian kadar protein, kadar lemak, dan SNF menggunakan alat lactoscan. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan 5 perlakuan lama simpan yaitu P1 (1 hari), P2 (3 hari), P3 (6 hari), P4 (9 hari), P5 (12 hari) penyimpanan dan 4 ulangan. Data yang diperoleh dianalisis dengan analisis of variance (ANOVA) dan dilanjut dengan uji duncan apabila terdapat pengaruh. Hasil penelitian menunjukkan adanya lama penyimpanan refrigerator mempengaruhi kadar protein, kadar lemak, dan SNF susu kambing pasteurisasi (P<0,05). Kadar protein, lemak dan SNF tertinggi pada penyimpanan P1 sebesar 4,26±0,01, 6,93±0,01, 8,30±0,01. Penyimpanan susu kambing pasteurisasi pada refrigerator selama 9 hari pada refrigerator menghasilkan kadar protein, kadar lemak dan SNF sesuai standar SNI. Kesimpulannya adalah penyimpanan susu kambing pasteurisasi pada refrigerator selama 9 hari masih dapat dikatakan aman untuk dikonsumsi.Kata kunci: susu kambing pasteurisasi, penyimpanan refrigerator, protein, lemak, solid non fat Content of Protein, Fat and Solid Non Fat Pasteurized Goat Milk in Refrigerator StorageAbstractThe aim of this research is to know the influence of refrigerator saving to protein content, fat content and solid non fat (SNF) of pasteurized goat milk. This research was conducted in Animal Production Technology Laboratory, Faculty of Animal Husbandry, Islamic University of Malang from July 01–August 21st, 2017. Goat milk, sugar, simple pasteurization, refrigerator, lactoscan were used in this research. The obtained fresh goat milk was pasteurized at 71.2°C for 15 seconds. The storage of pasteurized goat milk was done in the refrigerator for 12 days. During storage, protein content, fat content and SNF were observed. Testing protein levels, fat content, and SNF using lactoscan. The experimental design used a complete randomized design with 5 treatments, i.e. P1 (1 day), P2 (3 days), P3 (6 days), P4 (9 days), P5 (12 days) of storage. The data were analyzed by analysis of variance (ANOVA) and continued with Duncan test if there was significant effect. The results showed that refrigerator storage significantly influenced protein level, fat level, and SNF pasteurized goat milk (P< 0,05). The highest content of protein, fat and SNF was achieved at P1, i.e. 4.26±0.01, 6.93±0.01, 8.30±0.01, respectively. As conclusion, based on content of protein, fat and SNF pasteurized goat milk stored at refrigerator was still fulfill the requirement in SNI, thus the product was still safe for consumption.Keywords: pasteurized goat's milk, refrigerator storage, protein, fat, solid non fat
Perubahan Antosianin dan Aktivitas Antioksidan Tepung Uwi Ungu selama Penyimpanan Siti Tamaroh; Sri Raharjo; Agnes Murdiati; Sri Anggrahini
Jurnal Aplikasi Teknologi Pangan Vol 7, No 1 (2018): Februari 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (394.288 KB) | DOI: 10.17728/jatp.2224

Abstract

Uwi ungu (Dioscorea alata L) merupakan bahan pangan yang berpotensi sebagai sumber antioksidan alami, karena adanya komponen antosianin dan senyawa fenolik. Penelitian ini bertujuan mengetahui perubahan komponen tepung uwi ungu selama penyimpanan pada kondisi ruang simpan berbeda. Penyimpanan tepung uwi ungu disimpan pada ruang berukuran 60x60x60 cm3 yang diberi perlakuan lampu 150 lux (ruang KL) dan tanpa perlakuan lampu (TKL). Kelembaban udara (RH) ruang simpan dicatat pada saat penyimpanan. Penyimpanan dilakukan selama 25 hari, setiap 5 hari dilakukan uji pada kadar air, kadar antosianin, kadar fenolik, aktivitas antioksidan (%RSA). Data yang diperoleh diuji statistik dengan ANOVA, jika ada beda nyata diuji dengan DNMRT pada tingkat kepercayaan 5%. Hasil penelitian menunjukkan bahwa RH ruang simpan kondisi KL stabil (65%) dan RH ruang simpan TKL berubah-ubah (antara 77–85%). Penyimpanan pada ruang KL dapat menyebabkan peningkatan kadar air, penurunan kadar antosianin, penurunan kadar senyawa fenolik dan aktivitas antioksidan (%RSA) yang lebih rendah dibanding penurunan pada penyimpanan ruang TKL. Penyimpanan tepung uwi ungu pada hari ke-10 menunjukkan peningkatan kadar air, penurunan kadar antosianin, kadar fenolik dan aktivitas antioksidan yang signifikan. Komponen penyusun tepung uwi setelah penyimpanan 10 hari pada kondisi KL sebagai berikut; kadar air 15,5 %, kadar antosianin 56,24 mg/100 g bk, kadar senyawa fenolik 104,2 mg EGA/100 g bk dan aktivitas antioksidan 75,42%  (% RSA). Kesimpulannya, oleh karena ruang simpan KL terbukti menekan penurunan kadar air, kadar antosianin, kadar senyawa fenolik dan aktivitas antioksidan dibanding penyimpanan pada ruang TKL, maka tepung uwi ungu lebih baik disimpan dalam ruang KL.Antocyanin and Antioxidant Activity Purple Yam Flour Changes During StorageAbstractThe purple yam (Dioscorea alata L) is a food that has the potential as a source of natural antioxidants, because of the anthocyanin and phenolic component. The objective of this research is to know the change of purple yam flour component during storage at different  room storage. In this study, the purple yam flour was stored in the space size 60x60x60 cm3, with lamp 150 lux (LT) and without lamp (KLT). Room relative humidity (RH) was recorded. Purple yam flour was stored for 25 days. Analysis of the moisture content, anthocyanin concentration, phenolic concentration, antioxidant activity (% RSA ) was done in every 5 days. The data were statistically analized by ANOVA method, if there were significant difference were followed by Duncan Multiple Range Test at 5% confidence level. The results showed that KL provided stabil relative himidity (65%) and TKL provided much less stabil relative himidity (77–85%). Stored purple yam at KL room increased water content, decreased anthocyanin, phenolic compounds and antioxidant activity (% RSA). These changes generated lower value than that of in storage of TKL room. Storage purple yam flour on 10 days increased water content, decreased anthocyanin and phenolic levels, and antioxidant activity, significantly. Purple yam flour components after 10 days storage at KL room provided composition as follows: moisture content of 15.5%,  anthocyanin content of 56.24 mg/100g db,  phenolic compounds EGA of 104.2 mg/100 g bk and antioxidant activity of 75.42%. As conclusion, KL provided better storage of purple yam than TKL.
Karakteristik Fisik dan Kimia Tepung Malt Gabah Beras Merah dan Malt Beras Merah dengan Perlakuan Malting pada Lama Germinasi yang Berbeda Yasmin Aulia Rachma; Dina Yulia Anggraeni; Lita Lusiana Surja; Siti Susanti; Yoga Pratama
Jurnal Aplikasi Teknologi Pangan Vol 7, No 3 (2018): Agustus 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jatp.2707

Abstract

Gabah Beras Merah (GBM) dan Beras Merah (BM) merupakan bahan pangan yang memiliki nilai gizi yang tinggi dan bermanfaat bagi kesehatan. Malting merupakan proses pengolahan serealia dan biji-bijian untuk meningkatkan nilai gizinya dengan tahapan perendaman, germinasi, kemudian pengeringan. Penelitian ini bertujuan untuk mengetahui karakteristik densitas kamba, kelarutan, kadar air, dextrose equivalent, dan aktivitas antioksidan pada tepung malt GBM dan malt BM dengan perlakuan malting pada lama germinasi yang berbeda. Perlakuan malting dilakukan pada lama germinasi 0, 24, 48, dan 72 jam. Data dianalisis menggunakan pola regresi. Hasil penelitian menunjukkan bahwa lama germinasi pada proses malting mempengaruhi karakteristik kimia dan fisik tepung malt GBM dan tepung malt BM dengan korelasi sangat kuat. Pembuatan tepung malt BM menghasilkan tepung dengan karakter fisik dan kimia yang lebih baik dibanding GBM. Waktu tahap germinasi proses malting utamanya pada BM masih dapat dioptimalkan sehingga karakteristiknya masih berpotensi untuk ditingkatkan. Kesimpulannya, lama germinasi dapat mempengaruhi karakteristik kimia dan fisik tepung malt, baik untuk GBM maupun BM.Physical and Chemical Properties of Rough Red Rice Malt and Red Rice Malt Flour with Malting Treatment in Different Germination TimeRough red rice and red rice have high nutritional value and beneficial to health. Malting is a method of processing cereals and grains to increase its nutritional value by soaking, germination, and drying. This study aims to know the characteristic of bulk density, solubility, moisture content, dextrose equivalent, and antioxidant activity of rough red rice malt flour and red rice malt flour with malting treatment in vaious germination times. The data were analyzed using regression pattern with the treatment of malting germination time 0, 24, 48, and 72 hours. Results indicated that the various time of malting germination influenced the chemical and physical properties of rough red rice malt flour and red rice malt flour with very strong correlation. Red rice malt flour had better physical and chemical characteristics than rough red rice malt flour. Germination time in malting process specially for red rice still could be optimized. As conclusion, time of germination affected to the chemical and physical properties of malt flour from rough red rice and red rice.•|•|•
Karakteristik Fisik dan Kimia Gelatin dari Tulang Ikan Patin dengan Pre-Treatment Asam Sitrat Mega Pertiwi; Yoni Atma; Apon Zaenal Mustopa; Rizkia Maisarah
Jurnal Aplikasi Teknologi Pangan Vol 7, No 2 (2018): Mei 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.392 KB) | DOI: 10.17728/jatp.2470

Abstract

Penelitian ini bertujuan untuk melakukan ekstraksi gelatin dari tulang ikan patin menggunakan asam sitrat dan menganalisis karakteristik fisiko-kimianya. Ekstraksi gelatin melalui dua tahap yaitu tahap pre-treatment dengan asam sitrat dan ekstraksi utama dengan aquadest. Tahap pre-treatment dilakukan dengan variasi waktu yakni 24, 36, 48 dan 56 jam. Tahapan ekstraksi utama dilakukan pada suhu 45, 55, 65, dan 75°C. Hasil ekstraksi dilanjutkan dengan analisis keberadaan protein gelatin dengan metode SDS-PAGE. Analisis fisiko-kimia gelatin meliputi derajat keasaman (pH), rendemen, kekuatan gel, profil tekstur, viskositas, kadar air, kadar abu, kadar protein dan kadar lemak juga dilakukan. Hasil SDS-PGE gelatin perlakuan terbaik (yakni dengan pre-treatment 48 jam dan ekstraksi utama pada suhu 75°C) diketahui memiliki bobot molekul 162 kDa. Gelatin hasil ekstraksi terbaik memiliki rendemen sebesar 6,14%. Gelatin tulang ikan patin memiliki pH 4,46, kekuatan gel 364,19 bloom, daya kunyah sebesar 261,76 g dan viskositas 3,83 cP. Kadar proksimat gelatin tulang ikan patin meliputi kadar air, kadar abu, kadar protein dan kadar lemak masing-masing 7,72; 0,38; 58,70, dan 2,79%. Hasil penelitian juga menunjukan bahwa semakin tinggi suhu ekstraksi dan lamanya proses pre-treatment yang digunakan, maka bobot molekul protein menjadi semakin tinggi pula. Kesimpulannya, pre-treatment asam sitrat selama 48 jam dan ekstraksi utama pada suhu 75°C telah berhasil menunjukkan hasil yang paling baik dan dapat terkarakterisasi sifat fisik serta kimianya.Physical and Chemical Characteristics of Gelatin from Pangasius Catfish Bone with Pre-Treatment of Citric AcidThis research aimed to extract gelatin from Pangasius catfish bone using citric acid and then analyse its physicochemical characteristics. The extraction of gelatin was done in two stages soaking i.e pre-treatment with citric acid and main extraction by aquadest. Pre-treatment was done by soaking bones at 24, 36, 48 and 56 h, then the main extraction was done by soaked leached bones in temperature 45, 55, 65, and 75°C. The gelatin from catfish bone then was analyzed the presence of protein by SDS-PAGE and its physico-chemical including pH, yield, gel strength, texture profile, viscosity, moisture content, ash content, protein content, and fat content. Based on SDS-PAGE, fish bone gelatin had molecular weight of 162 kDa. The best extraction treatment (pre-treatment 48 h and main extraction at 75°C) produced 6.14% of gelatin yield. Gelatin of pangasius catfish bone had pH 4.46, gel strength of 353.76 bloom, chewing power of 261.76 g, and viscosity of 3.83 cP. Water, ash, protein and fat content was 7.72, 0.38, 58.70, and 2.79%, respectively. The higher extraction temperature and the longer time for pre-treatment process, the higher molecule weight of protein. As conclusion, the best treatment that was found in the sample with the pre-treatment at 48 h and the main extraction at 75˚C, was successfully characterized on its physicochemicals.•|•|•|•
Pengaruh Steam Blanching terhadap Aktivitas Polifenol Oksidase, Total Polifenol dan Aktivitas Antioksidan Biji Kakao Nurhayati Nurhayati; Djagal Wiseso Marseno; FMC Sigit Setyabudi; Supriyanto Supriyanto
Jurnal Aplikasi Teknologi Pangan Vol 7, No 3 (2018): Agustus 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jatp.2314

Abstract

Penelitian ini bertujuan untuk untuk mengetahui pengaruh steam blanching terhadap aktivitas polifenol oksidase, total polifenol, dan aktivitas antioksidan biji kakao. Bii kakao segar yang telah dihilangkan pulpnya ditimbang sebanyak 100 dan 200 g kemudian masing masing massa biji kakao diberi perlakuan steam blanching selama 1, 3, dan 5 menit. Biji kakao steam blanching dianalisa karakteristik fisikokimia (kadar air, pH, warna dan suhu), gugus fungsional (FTIR), aktivitas polifenol oksidase (PPO), kandungan total polifenol, dan aktivitas antioksidan. Hasil penelitian menunjukkan bahwa steam blanching dengan perlakuan kombinasi antara massa 100 g dan lama 1 menit menyebabkan menurunnya aktivitas PPO dari 445,87 menjadi 11,28 U/g berat biji kakao segar (FW), meningkatkan kandungan total polifenol dari 72,63 menjadi 92,15 mg Ekuivalen Asam Galat (EAG)/g bubuk kakao bebas lemak (DS), dan meningkatkan aktivitas penangkapan radikal bebas DPPH dari 55,36 % menjadi 68,99 % atau dengan nilai IC50 dari 3,39 menjadi 2,58 ml/l apabila dibandingkan dengan kontrol (tanpa steam blanching). Aktivitas antioksidan pada biji kakao steam blanching juga lebih tinggi dibandingkan antioksidan sintetis seperti BHT dan asam askorbat. Kesimpulannya, steam blanching dapat menurunkan aktivitas PPO, meningkatan kandungan total polifenol dan meningkatkan aktivitas antioksidan. Oleh karena itu, perlakuan steam blanching dapat dijadikan sebagai salah satu alternatif proses pengolahan dalam mempertahankan antioksidan alami polifenol biji kakao.Steam blanching effect on polyphenol oxidase  activity, total polyphenol content and antioxidant activity of cocoa beanThe aim of this research were to determine the effect of steam blanching on the activity of polyphenol oxidase, total polyphenol content, and antioxidant activity of cocoa beans. The fresh cocoa beans that have been removed the pulp were 100 and 200 g and then each cocoa beans mass was treated with steam blanching for 1, 3, and 5 minutes. Physicochemical characteristics (moisture content, pH, color, and temperature), functional group, polyphenol oxidase (PPO) activity, total polyphenol content and antioxidant activity of cocoa beans were observed. The results showed that steam blanching by treatment (100 g; 1 min) decreased of the PPO activity from 445.87 to 11.28 U/g of fresh weight (FW), increased total polyphenol content from 72.63 to 92.15 mg of Equivalent Galic Acid (EAG)/g defatted sample (DS), increased RSA DPPH from 55.36 % to 68.99 % or with IC50 value from 3.39 to 2.58 ml/l when compared to the control (without steam blanching). Antioxidant activity of steamed blanching cocoa beans also were detected higher than the synthetic antioxidants such as BHT and ascorbic acid. As conclusion, the steam blanching treatment might decrease the PPO activity, increase the total polyphenol content, and increase of antioxidant activity. Therefore, the steam blanching treatment could be used as an alternative process for preserving natural antioxidant of cocoa bean polyphenols.••
Ekstraksi Teh Putih Berbantu Ultrasonik pada Berbagai Amplitudo Asri Widyasanti; Tri Halimah; Dadan Rohdiana
Jurnal Aplikasi Teknologi Pangan Vol 7, No 3 (2018): Agustus 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.332 KB) | DOI: 10.17728/jatp.2295

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh amplitudo yang berbeda terhadap sifat fisikokimia ekstrak teh putih dengan menggunakan Ultrasound Assisted Extraction (UAE). Ekstraksi dilakukan menggunakan pelarut etanol 96% serta ukuran bubuk teh putih 18 mesh. Sonikasi digunakan pada amplitudo 25, 50, 75 dan 100%. Metode yang digunakan adalah percobaan laboratorium dengan analisis deskriptif. Parameter uji yang dilakukan meliputi rendemen total, kadar sisa pelarut, bobot jenis, warna, dan kadar polifenol. Hasil penelitian menunjukkan perbedaan penggunaan amplitudo pada proses ekstraksi mempengaruhi nilai rendemen ekstraksi dan mutu ekstrak dengan penggunaan amplitudo 100% sebagai perlakuan terbaik dengan nilai rendemen total sebesar 67,35%; nilai bobot jenis filtrat 0,8241; bobot jenis ekstrak sebesar 1,0306; dan kadar polifenol sebesar 0,90 mg asam galat ekivalen/g bobot kering. Hasil menunjukkan nilai yang lebih baik dibandingkan dengan maserasi dengan rendemen ekstraksi hasil maserasi 60,12 %; nilai bobot jenis filtrat 0,8163; bobot jenis ekstrak sebesar 1,0053; dan kadar polifenol sebesar 0,64 mg asam galat ekivalen/g bobot kering. Karakteristik warna ekstrak etanol teh putih seluruh perlakuan amplitudo dan maserasi menghasilkan kromatisitas warna merah. Kesimpulan dari penelitian ini adalah adanya pengaruh teknik sonikasi pada sifat fisikokimia ekstrak teh putih dan ekstraksi menggunakan amplitudo 100% menghasilkan nilai rendemen dan kadar polifenol terbaik. Extraction of White Tea by Ultrasound Assisted Extraction at Various AmplitudeAbstractThe purpose of this study was to determine the effect of different amplitudes on physicochemical properties of white tea extract using Ultrasound Assisted Extraction (UAE). Ethanol was used as solvent and material size was 18 mesh. This research used amplitude: 25, 50, 75, and 100%. The method used laboratory experiment using descriptive analysis. The parameters were total extraction total yield, residual solvent content, specific gravity, colour, and total polyphenol. The result showed that the amplitude in extraction process influenced the yield and the quality of the extract. As much as 100% amplitude was the best treatment resulted in total yield of 67.35%; the specific gravity of filtrate was 0.8241; the specific gravity of extract was 1.0306; and the polyphenol content was 0.90 mgGAE /g dryweight. The result of UAE showed a better result than hot maceration with total yield of 60.12%; specific gravity of filtrate was 0.8163; specific gravity of extract was 1.0053; and polyphenol content was 0.64 mgGAE /g dry weight. Colour characteristics of white tea ethanol extracts from all operating amplitude and hot maceration were resulting in red colour. As conclusion, ultrasound technique provided the physicochemical properties changes of extract and 100% amplitude was resulting best value of yield and polyphenol content.••••••
Perubahan Aktivitas Antioksidan dan Total Mikroba Salak Pondoh Selama Penyimpanan dengan Asam Hypoiodous (HIO) Wildan Alfira Gusrianto; Siti Fatimah-Muis; Ahmad Ni&#039;matullah Al-Baarri; Anang Mohamad Legowo
Jurnal Aplikasi Teknologi Pangan Vol 7, No 3 (2018): Agustus 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.184 KB) | DOI: 10.17728/jatp.2874

Abstract

Penelitian ini bertujuan untuk mengetahui efek asam hypoiodous (HIO) pada aktivitas antioksidan dan total mikrobia pada salak Pondoh selama penyimpanan pada suhu ruang. Metode eksperimen yang digunakan untuk penelitian ini adalah rancangan acak kelompok dua perlakuan dan tiga kali ulangan dengan total sampel sebanyak 45 salak Pondoh dengan minimum usia panen adalah sekitar 5 bulan dengan berat sebesar 65±7 g per salak. Penyemprotan HIO dilakukan satu kali perhari dan dengan volume sebanyak 0,5 ml. Setelah penyemprotan, dilakukan penyimpanan pada suhu ruang selama 12 hari. Pengukuran aktivitas antioksidan dan total mikroba dilakukan setiap dua hari sekali. Analisis data menggunakan Shapiro-Wilk, Independent t-test, dan Mann-Whitney. Hasil penelitian menunjukkan bahwa selama penyimpanan, telah terjadi peningkatan aktivitas antioksidan pada salak mulai dari 21,7±1,72% hingga kisaran 82,4% untuk tanpa dan dengan perlakuan HIO serta terdapat peran HIO yang sangat nyata dalam menurunkan total mikroba pada salak pondoh HIO. Kesimpulannya, HIO dapat digunakan untuk agen yang dapat meningkatkan antioksidan serta dapat digunakan untuk menekan perkembangan mikroba pada salak.Hypoiodous Acid Effect on Antioxidant Activity and Total Microbes in Salak Pondoh in Room Temperature of StorageAbstract This study was aimed at analyzing the influence of hypohiodous acid or HIO on antioxidant activity and total microbes in salak pondoh during storage at room temperature. This study used a group randomized design with two treatments and three replications. Each group consisted of 45 salak Pondoh which was harvested at 5 months age with size 65±7 g/salak. The spraying of 0.5 ml of HIO was done everyday for entire of salak surface, then salak was stored at 25ºC for 12 days. The measurement of antioxidant activity and total microbes was performed every two days. Data were analized using Shapiro-Wilk, Independent t-test, and Mann-Whitney. Result indicated that HIO was able to increase antioxidant activity from 21.7±1.72 to about 82% for control and HIO treated salak. HIO was also inhibit growth total microbes significantly. As conclusion, HIO might be used as an agent to increase antioxidant activity and inhibit total microbe in salak.
Pemanfaataan Limbah Padat Industri Tahu sebagai Co-Subtrat untuk Produksi Biogas Fredynanta Saputra; Sutaryo Sutaryo; Agung Purnomoadi
Jurnal Aplikasi Teknologi Pangan Vol 7, No 3 (2018): Agustus 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.251 KB) | DOI: 10.17728/jatp.2315

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penggunaan ampas tahu sebagai co-substrat dalam digesti secara anaerob terhadap kecernaan protein, konsentrasi VFA dan total amonia nitrogen. Penelitian dilakukan dengan menggunakan dua buah digester kontinyu dan data dikoleksi selama tiga kali hydraulic retention time (HRT), dimana satu kali HRT setara dengan 25 hari. Data yang diperoleh dibahas dengan metode independent sampel comparison dengan membandingkan variabel hasil pengamatan dari digester satu (tanpa ampas tahu) dan digester dua (penambahan 5% ampas tahu) yang keduanya diencerkan menggunakan air dengan perbandingan 1:1. Hasil dari penelitian ini adalah terdapat adanya perbedaan yang nyata (P<0,05) dari perlakuan yang diterapkan terhadap kecernaan protein, konsentrasi VFA, dan konsentrasi total amonia nitrogen (TAN). Nilai kecernaan protein, konsentrasi VFA dan konsentrasi TAN dari digester 1 dan digester 2 secara berturut turut adalah 36,13% dan 25,71%; 25,39 ml/mol/l dan 11,21 ml/mol/l serta 1959 dan 1675 mg/l. Kesimpulan dari penelitian ini adalah konsentrasi VFA dan TAN yang stabil pada konsentrasi yang relatif rendah pada slurry dari digester 2 dipertengahan dan akhir penelitian mengindikasikan bahwa ampas tahu bisa digunakan sebagai co-subtrat pada feses sapi, namun demikian perlu dilakukan penelitian lebih lanjut untuk mengevaluasi level ampas tahu yang terbaik untuk meningkatkan produksi biogas dari digester biogas berbasis feses sapi.Utilization of Waste from Tofu as Co-Substrate in Biogas ProductionAbstractThe aim of this research was to evaluate the effect of co-substrate of waste of tofu in anaerobic digestion on protein digestibility, VFA concentration, and total ammonia nitrogen. The experiment was performed in two continuously feeding digesters for three hydraulic retention times (HRT) which was a HRT equal to 25 d. The observed data was analysed using independent sample comparison. The treatments were digester 1 as no co-substrat and digester 2 as 5% solid waste from tofu addition which both of them then diluted with tap water at 1:1 ratio. The results of this study showed that there were significant effect (P<0.05) of treatments on protein digestibility, VFA concentration and total ammonia nitrogen. The protein digestibility, VFA concentration and TAN concentration of digester 1 and digester 2 were 36.13 and 25.71%; 25.39 and 11.21 ml/mol/L; 1959 and 1675 mg/L, respectively. As conclusion, a stabil at low concentration of VFA and TAN in the middle and in the end experiment might be used to indicate that waste from tofu is suitable substrate for co-digestion with cow feses, however a further experiment is needed to obtain optimum level of tofu cake to enhance biogas production of digester biogas base on cow feses.•••

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