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INDONESIA
Bioma : Berkala Ilmiah Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 25982370     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
BIOMA (ISSN 1410-8801) adalah Majalah Ilmiah Biologi yang bertujuan mewadahi semua informasi hasil penelitian, telaah pustaka, makalah teknis, dan kajian buku, dari berbagai cabang ilmu Biologi. Diharapkan penerbitan ini dapat berkontribusi dalam penyampaian informasi ilmiah yang merupakan mata rantai proses kegiatan ilmiah. Terbit sejak tahun 1999.
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol. 24, No 2, Tahun 2022" : 8 Documents clear
Eksplorasi Jamur Desa Tulung Agung, Lampung Timur dan Potensi Pemanfaatannya Sari, Okta Yulia; Mahardhika, Wahyu Aji; Afsari, Nadhila Mutia; Putra, Ivan Permana
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.24.2.105-119

Abstract

Mushrooms an be found in various places, such as litter, rotten wood, trees, and soil around residential areas. Tulung Agung Village in East Lampung Regency has various kinds of vegetation and several other abiotic factors that support the growth of mushroom. The study of mushroom’s exploration could elaborate the knowledge about mushroom diversity in resdential areas. This study aims to explore the types of mushrooms based on the description of their fruiting bodies from the residential location of Tulung Agung Village, District Pekalongan, East Lampung Regency, Lampung. The methods used were purposive sampling technique in a shady area or under the shade of a tree, documenting the mushroom fruiting bodies hat were found, and presenting mushrooms with fruiting bodies characters. After the exploration, 18 species from 14 genera of mushrooms were found, including Auricularia auricula-judae, Collybia sp., Conocybe sp., Daldinia sp., Gymnopilus sp., Marasmius sp., Marasmius haematocephalus, Mycena sp., Lepiota sp., Parasola sp., Pycnoporus sp., Trametes sp., Tremellodendron sp., and Xylaria sp. The various potentials of each type of mushrooms that were found are for food sources, traditional medicines, biodegradation of contaminants, sources of bioluminescence, and sources of various bioactive compounds. The Mushroom that were found need molecular identification to get the correct species so that the potential of Mushroom can be utilized optimally by residents based on more specific type information.
Antibacterial Activity of Freshwater Sponge Oncosclera asiatica Against Escherichia coli and Staphylococcus aureus Wulandari, Dyah; Dewantoro, Giwang; Lunggani, Arina Tri; Suprihadi, Agung; Riani, Catur; Setiawan, Edwin; Farikha, Siti Lutfiatul; Budiharjo, Anto
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.24.2.120-129

Abstract

Freshwater sponges are animals from the Porifera phylum that live in freshwater. The sponge used is Oncosclera asiatica was taken from Kali Porong, East Java. Seventeen isolates of bacteria have been obtained from isolation. Antibacterial potential testing was performed by paper disc inhibition assay using Escherichia coli and Staphylococcus aureus as pathogenic bacteria and amoxicillin as a positive control. The antibacterial activity test showed that four isolates have the potential activity. The isolates with the highest inhibition zones were identified using a 16S rRNA.The results of BLAST showed isolate number 2 was Pseudomonas moraviensis with 99.51% similarity. The phylogenetic tree analysis was build using the MEGA X program. The results of the phylogenetic tree analysis showed that P.moraviensis had a bootstrap value of 100% with a  genetic distance value of 0.001. P. moraviensis isolates screened for the presence of Non-Ribosomal Peptide Synthetase (NRPS) gene by A2gamF and A3gamR primers. The amplification result from NRPS gene showed positive meaning that P.moraviensis genome contained NRPS gene.
Respon Histologis Aorta dan Jantung Rattus Norvegicus Hiperlipidemia Setelah Pemberian Jus Buah Kersen (Muntingia calabura L.) dan Ekstrak Daun Lakum (Cayratia trifolia L.) Agustina, Enyda; Saraswati, Tyas Rini; Tana, Silvana
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.24.2.96-104

Abstract

This study aims to determine the histological features of the aorta and heart of male Rattus norvegicus after  administration of cherry juice (Muntingia calabura L.) dan lakum leaf extract  (Cayratia trifolia L.). The study used 30 male Rattus norvegicus L.  in a Completely Randomized Design (CRD) consisting of 5 treatments with 6 repetitions, namely the control group (P0), the standard feeding group (P1), the hyperlipid feed group + 0.2 mL/200 g BB cherry juice (P2), the hyperlipid feed group + 40 mg / 200 g BB lakum leaf extract (P3) and hyperlipid feed group + 0.18 mg / 200 g BB simvastatin (P4). Hyperlipid feed was given during the treatment, which was 28 days. To obtain histological data, preparations were made using the paraffin method and then observed using a photomicrograph with 400x magnification and observing the aortic lumen, foam cells and intracellular accumulation of lipids in smooth muscle. Data were analyzed by ANOVA followed by Duncan Test at a significant level of 5%. The results showed that the addition of cherry juice and lakum leaf extract gave a significant effect on lumen width and thickness of the aortic wall (p <0.05), while on changes in heart muscle cell size no significant effect (p> 0.05). Histomorphometri showed the presence of foam cells and accumulation of smooth muscle lipids in the control and group treatments with hyperlipid feeding. The conclusion of this study was that the addition of cherry juice  and lakum leaf extract can reduce atherosclerotic lesions by eliminating showed foam cells and prevent proliferating smooth muscle cells so the size of the aortic lumen width returns to normal, but didn’t cause enlargement of the heart muscle (cardiomegaly).
Pengamatan Morfologi Bagian Tanaman Lima Kultivar Kedelai [Glycine max (L.) Merrill] Sumarmi, S; Triyono, Kharis
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.24.2.130-137

Abstract

Written descriptions of soybean plants often have different results after planting. Not much has been done to observe the morphology of the anthers of various soybean cultivars. Not much has been done to observe the morphology of the anthers of various soybean cultivars. This study aims to observe the morphological characteristics of five soybean cultivars.  The research was conducted in the research field and plant laboratory at the Faculty of Agriculture, Universitas  Slamet Riyadi, Surakarta, using a completely randomized design. Five soybean cultivars were planted namely Argomulyo, Grobogan, Wilis, Anjasmoro and Hitam Malika. Observations on the seeds used, plant height growth, leaf morphology, flower morphology and anther conditions. Argomulyo, Grobogan and Anjasmoro cultivars have large seeds, weighing 100 seeds between 15-18 grams. Plant height growth occurs until the plant is 5 weeks old. The color and shape of the leaves of five one-month-old soybean cultivars are almost the same. At the beginning of the 2nd month, the leaves of the soybean plants turned dark green, which was evident in Grobogan and Hitam Malika cultivars. The crown color of the Black Malika soybean flower is dark purple, different from the other 4 cultivars with purple flowers. Cultivar Anjasmoro, 5 weeks old, reached 63 cm, the highest among the five cultivars planted. Each soybean cultivar has morphological characteristics in terms of plant height, shape, leaf color, flowers, pods, seeds and or anthers.
Potensi Rizobakteri Pembentuk Endospora dari Brokoli (Brassica oleracea var. Italica) sebagai Agen Biokontrol Ralstonia solanacearum serta Biofertilizer Fauzaan, Muhammad Faishal; Wijanarka, Wijanarka; Kusdiyantini, Endang; Budiharjo, Anto; Ferniah, Rejeki Siti
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jis.%v.%i.%Y.210-226

Abstract

Penyakit Layu Bakteri merupakan penyakit tular tanah yang menyerang tanaman hortikultura yang disebabkan oleh bakteri fitopatogen Ralstonia solanacearum. Komunitas bakteri tanah yang menghuni rizosfer tanaman diketahui memiliki potensi yang baik dalam mendukung pertumbuhan maupun melindungi tanaman dari serangan patogen. Bakteri pembentuk endospora dipilih karena memiliki toleransi yang cukup bagus apabila dihadapkan dengan lingkungan yang tidak cocok bagi pertumbuhannya. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi isolat rizobakteri pembentuk endospora potensial sebagai agen biokontrol melawan R. solanacearum dari rizosfer brokoli sehat di lahan pertanian organik desa Kopeng Kabupaten Semarang serta mengetahui potensinya sebagai agen biofertilizer. Isolasi rizobakteri pembentuk endospora menggunakan metode spread plate; purifikasi isolat rizobakteri menggunakan metode streak plate, karakterisasi dengan uji pewarnaan Gram dan uji pewarnaan endospora, uji antibakteri melawan R. solanacearum menggunakan metode Kirby-Bauer; identifikasi molekuler dengan gen 16S rRNA; konstruksi pohon filogenetik dan uji potensi biofertilizer berupa uji produksi IAA menggunakan reagen Salkowski, uji pelarutan fosfat menggunakan media Pikovskaya dan uji fiksasi nitrogen menggunakan media Nitrogen-free Bromothymol-blue. Hasil penelitian diperoleh 18 isolat rizobakteri pembentuk endospora; isolat RB5 memiliki potensi antibakteri dengan daya hambat kategori sedang (6 mm) dan teridentifikasi sebagai Bacillus subtilis strain FP.PT.1.1-CTCRI dengan nilai similaritas sebesar 98,10% serta mampu memroduksi IAA dan melarutkan fosfat tetapi tidak mampu memfiksasi nitrogen secara kualitatif.
Analisis Kondisi Kesehatan Ekosistem Mangrove Di Pantai Takari Kabupaten Bangka Farhaby, Arthur Muhammad; Anwar, Muhammad Syaiful
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.24.2.147-154

Abstract

The coastal area is a transitional ecosystem that is influenced by land and sea, which includes several ecosystems, one of which is the mangrove forest ecosystem. The area of the Bangka Belitung Islands Province has a coastal area with a very wide mangrove cover, with an area of around 273,692.81 Ha. The degradation of mangrove forests in Indonesia is caused by various factors, namely: the number of unconventional tin mining activities that are widely carried out by the community. For this reason, a management effort is needed that includes an ecological monitoring effort on the condition of the mangrove community in an area. The purpose of this study was to determine the health condition of mangrove forests in Takari Beach, Bangka Regency as the basis for determining sustainable mangrove management policies. This research was conducted in August-September 2022 at Takari Beach. The results obtained from this study are that Takari Beach has a cover percentage of around 39.20% to 44.52%, where at that location there are 5 species, namely: Rhizophora apiculata, Sonneratia caseolaris, Rhizophora mucronata, Avicennia alba and Avicennia lanata. The health condition of mangroves on Takari Beach is categorized as rare/damaged. These conditions can be influenced by human activities in coastal areas such as marine mining activities or changing natural factors such as temperature, salinity, pH, DO and others. Keyword :  Healt Condition, Mangrove, Percentage Cover, Takari Beach Abstrak Wilayah  pesisir  merupakan  ekosistem  transisi  yang  dipengaruhi daratan  dan  lautan,  yang mencakup  beberapa  ekosistem,  salah  satunya  adalah  ekosistem  hutan  mangrove. Wilayah Provinsi Kepulauan Bangka Belitung memiliki kawasan pesisir dengan tutupan mangrove yang begitu luas yaitu dengan luas sekitar 273.692,81 Ha. Degradasi hutan mangrove di Bangka Belitung disebabkan oleh berbagai faktor, yaitu: banyaknya dijumpai aktivitas – aktivitas antropologis terutama konversi lahan mangrove menjadi pemukiman, tambak, dan tambang  Untuk itu, diperlukan sebuah upaya pengelolaan yang mencakup di dalamnya usaha pemantauan ekologi terhadap kondisi komunitas mangrove di suatu kawasan. Tujuan dari penelitian ini yaitu untuk mengetahui kondisi kesehatan hutan mangrove di Pantai Takari Kabupaten Bangka sebagai dasar penentuan kebijakan  pengelolaan  mangrove  berkelanjutan.  Penelitian  ini  dilakukan  pada  bulan  Agustus- September 2022 di Pantai Takari. Hasil yang diperoleh dari penelitian ini yaitu Pantai Takari memiliki persentase tutupan sekitar  39,20%  sampai 44,52%, Dimana pada lokasi tersebut terdapat 5 spesies yaitu : Rhizophora apiculata, Sonneratia caseolaris, Rhizophora mucronata, Avicennia alba dan Avicennia lanata  .  Kondisi kesehatan  mangrove  di  Pantai Takari  termasuk  kategori jarang/rusak. Kondisi tersebut dapat dipengaruhi oleh kegiatan manusia dikawasan pesisir misalnya aktivitas pertambangan laut atau faktor alam yang berubah-ubah seperti suhu, salinitas, pH, DO dan lain-lain. Kata Kunci: Mangrove, pantai takari, persentase tutupan, kondisi kesehatan.  
Uji Kesetaraan Aktivitas Antioksidan Seduhan Bunga Kenop (Gomphrena globosa L.) dengan Vitamin C Menggunakan Metode DPPH Rohmah, Martina Kurnia; Yulianti, Ella Rahman; Ambari, Yani
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.24.2.155-161

Abstract

Steeping of Kenop flower (Gomphrena globosa L.) is widely used as a health herbal drink that contains betacyanin and flavonoid compounds that act as antioxidants. This study aims to determine the equivalence of vitamin C levels in steeping of Kenop Flower (Gomphrena globosa L) using the DPPH method. The DPPH method is a simple, fast, inexpensive and can be used to measure the ability of an antioxidant. Data analysis of the absorbance of the steeping of the Kenop flower was calculated using TAC (Total Antioxidant Capacity Index). Phytochemical screening of infusion of the positive Kenop flower contains alkaloids, flavonoids, saponins, tannins and triterpenoids. Kenop flower is made with various concentrations of 5, 10 and 15 gr is steeping in 100 ml aquades. The antioxidant activity of steeping of Kenop flower 50, 100, and 150 mg/ml was equivalence with vitamin C respectively at 9,71 ppm (0,009 mg/ml) 13,96 ppm (0,013 mg/ml), and 15,18 ppm (0,015 mg/ml).
Produksi dan Ekstraksi Pigmen Anka Monascus purpureus pada Beras IR42 Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.24.2.162-165

Abstract

Monascus merupakan kapang filamentous penghasil pigmen yang telah lama digunakan sebagai pewarna alami pangan, terutama M. purpureus dan M. ruber. Anka merupakan kultur padat dari M. purpureus dengan media beras. Tujuan penelitian ini untuk analisis pigmen merah, oranye dan kuning dari Anka M. purpureus. Beras IR42 digunakan untuk pembuatan Anka yang diinkubasi selama 30 menit. Ekstraksi pigmen menggunakan solven metanol dan diukur dengan spektrofotometer pada panjang gelombang 500 nm untuk pigmen merah, 470 nm untuk oranye dan 390 nm untuk pigmen kuning. Anka yang dihasilkan berwana merah yang menunjukkan adanya pertumbuhan M purpureus. Konsentrasi pigmen tertinggi diperoleh dari pigmen kuning sebesar 1.121 CVU/g, sedangkan pigmen merah sebesar 1.049 CVU/g dan yang paling rendah adalah pigmen oranye sebesar 837 CVU/g.

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