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Bioma : Berkala Ilmiah Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 25982370     DOI : -
BIOMA (ISSN 1410-8801) adalah Majalah Ilmiah Biologi yang bertujuan mewadahi semua informasi hasil penelitian, telaah pustaka, makalah teknis, dan kajian buku, dari berbagai cabang ilmu Biologi. Diharapkan penerbitan ini dapat berkontribusi dalam penyampaian informasi ilmiah yang merupakan mata rantai proses kegiatan ilmiah. Terbit sejak tahun 1999.
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Articles 318 Documents
Pengaruh Dosis Kompos dengan Stimulator Trichoderma terhadap Pertumbuhan dan Produksi Tanaman Jagung (zea mays l.) Varietas Pioner -11 pada Lahan Kering Afitin, Retno -; Darmanti, Sri -
Bioma : Berkala Ilmiah Biologi Vol. 11, No. 2, Tahun 2009
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.765 KB) | DOI: 10.14710/bioma.11.2.69-75

Abstract

Lahan kering di Indonesia sangat luas. Sifat lahan kering yang kurang subur dengan tingkat erosi yang tinggidan rendahnya pasokan air menyebabkan pemanfaatan lahan kering belum optimal. Kompos sebagai salah satupupuk organik dengan bahan baku yang tersedia cukup banyak merupakan alternatif yang tepat untuk digunakandalam memanfaatkan lahan kering sebagai lahan pertanian. Penelitian menggunakan Rancangan Acak Lengkapdengan 4 perlakuan berupa dosis pemupukan , yaitu : D0 : 0 ton / Ha (kontrol), D1 : 15 ton / Ha, D2 : 30 ton / Hadan D3 : 45 ton / Ha. Data yang diperoleh dianalisis dengan Analisis of Varians (Anava), bila terdapat beda nyatadilanjutkan dengan uji Beda Nyata terkecil (BNT) pada taraf kepercayaan 95%. Dari hasil penelitian dapatdisimpulkan bahwa kompos dengan stimulator tricoderma berpengaruh meningkatkan pertumbuhan dan produksitanaman jagung pada lahan kering dan dosis 3 kg / m2 merupakan dosis optimal untuk pertumbuhan dan produksijagung.
Pelacakan Gen Sitokrom Oksidase Subunit 1 (Co1) DNA Mitokondria Pada Itik Tegal (Anas sp.) Rahayu, Annisa Rizky; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.763 KB) | DOI: 10.14710/bioma.18.2.114-122

Abstract

Itik Tegal adalah salah satu sumber plasma nutfah ternak Indonesia yang belum memiliki informasi asal usul dan identitas genetik, sedangkan ini sangat diperlukan sebagai dasar dalam usaha persilangan dan pemuliaan untuk menghasilkan itik hibrida unggul. Penelitian ini bertujuan untuk mengetahui identitas genetik itik Tegal menggunakan gen sitokrom oksidase subunit 1 (CO1) dengan primer BirdF1 dan BirdR1. Penelitian dilakukan dengan cara isolasi DNA dari otot paha itik, diikuti amplifikasi gen CO1, dan sekuensing. Sekuen gen CO1 digunakan untuk analisis hubungan kekerabatan dengan mengkonstruksi pohon filogenetik menggunakan metode neighbor-joining dengan analisis bootstrap 1.000 ulangan. Model Kimura 2-parameter digunakan untuk menghitung jarak genetik dengan pairwise distance. Hasil penelitian memperoleh fragmen gen CO1 itik Tegal. Fragmen tersebut homolog dengan sekuen gen CO1 Anas platyrhynchos voucher NHMO-BC400. Analisis filogenetik menunjukkan itik Tegal memiliki hubungan kekerabatan paling dekat dengan A. platyrhynchos yang terdistribusi di Skandinavia dan Amerika Utara, A. poecilorhyncha yang terdistribusi di Asia tropis dan timur, serta Tadorna tadorna yang terdistribusi di China. Kata kunci: gen CO1, genetik, itik Tegal
Perilaku Vektor Malaria Anopheles farauti Laveran (Diptera: Culicidae) Di Ekosistem Pantai (Kabupaten Biak Numfor) Dan Ekosistem Rawa (Kabupaten Asmat) Propinsi Papua Kawulur, Hanna; Soesilohadi, RC Hidayat; Hadisusanto, Suwarno; Trisyono, Andi
Bioma : Berkala Ilmiah Biologi Vol. 17, No.1, Tahun 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.599 KB) | DOI: 10.14710/bioma.17.1.34-40

Abstract

Biak Numfor and Asmat districts reported as malaria endemic areas in Papua (Papua Global Fund, 2011). Anopheles farauti mosquito is one of the malaria vectors in the region. Malaria control efforts have been made but there are still many cases of malaria. Malaria control will provide maximum results if there is a match between the vector behavior and programs undertaken. The purpose of this study was to determine the bionomics factors (behavioral) of malaria vector An. farauti mosquitoes in coastal ecosystems (Biak Numfor) and swamp (Asmat); namely: (a). density (b) age and (c) blood-seeking behavior. The method used in this study is human landing collection, resting collection and ELISA blood-feed. The results showed that the population density of An. farauti mosquito did not have a positive relationship with the number of malaria patients in both study areas. The activity of An. farauti mosquito on blood-seeking at the coastal and swamp ecosystems lasted all night but mainly at 18:00 to 19:00 hour. The study also showed that blood-seeking activity mostly done outside of the house. Age estimation of An. farauti mosquito population at coastal ecosystems ranging from 16-18 days, while in the swamp ecosystem is 12-14 days. Population density, approximately age and blood-seeking behavior indicates that An. farauti mosquitoes on the coastal and swamp ecosystems have the potential to be an effective malaria vector.   Keywords: Malaria, Vector, Behavior
Karakterisasi Senyawa Flavonoid Ekstrak Polar Daun Gamal Kultivar Lampung Utara Dan Uji Aktivitasnya Terhadap Kutu Putih Kakao (Planococcus minor, Hemiptera: Pseudococcidae) Nukmal, Nismah; Pasutri, Agata Yelin; Pratami, Gina Dania
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.705 KB) | DOI: 10.14710/bioma.21.1.25-34

Abstract

Cocoa is one of the export commodities that have great prospects. The quality of cocoa in Indonesia are not satisfactory, this is caused by cocoa mealybug (Planococcus minor). Gamal (Gliricidia maculata) contains flavonoid compounds which can be used as bioinsecticides. The aims of research to determine the characteristic of flavonoid compounds in gamal leaf powder which is effective for kill of cocoa mealybug P. minor. Extraction and spectroscopy analysis (UV-Vis and FTIR) were done in the Integrated Laboratory of Technology Innovation Center (ILTIC) and the bioassay was done at Zoology Laboratory of Unila. The results shows that the crude water extract of gamal leaf powder North Lampung Cultivar (NLC) more effective killed lower than purified water extract. It was indicated by value of LC50.72hours the crude water extract than purified water extract (0.11%: 0.27%). The flavonoid compounds of gamal leaf powder NLC have the characteristics of blue fluorescence in UV light λ 254 and 366 nm. The flavonoid with the maximum peak of wavelength 310 could be grouped to flavonone with structural frame O-H, C=O carbonyl, aromatic C=C, and C-O.
Dinamika Populasi Wideng (Sesarma spp) dan Tangkapan (Populasi) Scylla di Kawasan Mangrove Tapak, Tugurejo Semarang : Suatu Kajian Pemberdayaan Predator untuk Mengendalikan Wideng Hama Bibit Mangrove Berbasis Manajemen Ekosistem Hidayat, Jafron Wasiq; Anggoro, Sutrisno; Hendrarto, Ign Boedi
Bioma : Berkala Ilmiah Biologi Vol. 14, No. 2, Tahun 2012
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.673 KB) | DOI: 10.14710/bioma.14.2.49-63

Abstract

Kondisi kerusakan hutan mangrove sudah sangat memprihatinkan. Gangguan hama wideng (Sesarma spp) mempengaruhi keberhasilan penghijauan. Wideng merupakan hama minor yang dapat bergeser sifatnya jika populasinya tinggi pada kondisi tertentu. Oleh karena itu perlu dilakukan penelitian tentang dinamika populasi wideng, sehingga dapat mengetahui waktu kapan wideng berpotensi menjadi hama. Kajian yang sama juga ditujukan terhadap pemangsa potensialnya yaitu Scylla yang ditujukankan untuk mengetahui tekanan  predator terhadap wideng dan peluang pemberdayaannya sebagai pakan budidaya. Penelitian bersifat observasional yang dilakukan di kawasan mangrove Tapak, Tugurejo. Sampling dilakukan di enam stasiun yang dipilih secara justified random sampling. Kajian eksperimental menggunakan Rancangan Acak Lengkap(RAL) dengan tiga perlakuan dan tiga ulangan yang dilanjut dengan uji Beda Terkecil. Hasil memperlihatkan bahwa populasi wideng mengikuti pola unimodal dengan puncak populasi pada bulan Nopember mencapai 30/m2.Kepiting Scylla memasuki musim tangkap sejak Oktober hingga Maret dan mencapai puncaknya pada bulan Pebruari. Kepadatan populasi tersebut selaras dengan keberadaan wideng yang juga tinggi populasinya, sehingga dapat dipergunakan sebagai pakan budidaya sekaligus menekan sifat hamanya.Kepiting bakau menyukai wideng pada semua ukuran, dimana tidak membedakan ukuran badan wideng mangsanya  pada hari ke-5, meskipun pada hari ke-3 memperlihatkan kecenderungan menyukai ukuran badan yang kecil; sehingga wideng bersifat fleksibel sebagai pakan budidaya Scylla.   Kata kunci : Dinamika populasi, wideng, Scylla spp, tingkat predasi
Abundance and Diversity of Bryofauna in Coffee and Tea Vegetations Rahadian, Rully; Perwati, Lilih Khotim; Baskoro, Karyadi
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.764 KB) | DOI: 10.14710/bioma.18.2.1-6

Abstract

Forest alteration into agroecosystem affect many organisms including bryofauna which their survivorship depend much on their host. Coffee and tea plantation are common agroecosystem in Indonesia. So far, the effect of habitat alteration on Bryofauna community have not been known yet. The objective of this study is to determine community structure attributes i.e., abundance, diversity and composition of bryofauna living in coffee and tea vegetation. The study was located in Ungaran Mountain, District of Semarang Indonesia. Bryofauna were extracted from moss using Tullgren funnel method and were identified until possible taxa. Bryofauna sampling was conducted both in coffee and tea vegetation. Bryofauna were extracted from the moss using Tullgren funnel method. The result shows that Acari was the most abundant both in coffee and tea area. Generally, bryofauna was more abundant in the coffee vegetation than in the tea vegetation. The diversity of bryofauna was not affected neither by altitude nor vegetation. However, the diversity of bryofauna seems affected more by their host, bryophyte. Keywords: Bryofauna, Acari, coffee and tea vegetation.
Eksplorasi Kandungan Klorofil pada beberapa Sayuran Hijau sebagai Alternatif Bahan Dasar Makanan Tambahan Setiari, Nintya -; Nurchayati, Yulita -
Bioma : Berkala Ilmiah Biologi Vol. 11, No. 1, Tahun 2009
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (44.214 KB) | DOI: 10.14710/bioma.11.1.6-10

Abstract

Chlorophyll is a plant pigment which recently have been popular as food supplement. The source ofchlorophyll consumed now are alfalfa’s leaves algae such as Spirulina and Chlorella.. Chlorophyll content find in allkinds of plant especially vegetables so that have potent to be source of chlorophyll. The aims of this research are toexplore alternative source of chlorophyll aside of Leguminosae, from vegetables such as sweet basil, spinach, waterspinach, cassava leaves, pegagan, and papaya’s leaves and to study the vegetables potent to be food supplement.The experiment has been conducted by extracting 0,1 g each leaves in 10 mL acetone 85%. The extract was filteredwith Whatman paper No. 1, thus record the absorbance by using spektrofotometer UV Vis. Statistical analysis wasused to analyze chlorophyll content is Analysis of Variance (ANOVA) with Randomized Complete Design (RCD) in95% significancy. If the ANOVA showed significantly different, the analysis were continued with Duncan’s test.The result showed that the highest content of chlorophyll-a, b, and total found in papaya’s leaves. However thelowest one was found in sweet basil. Papaya’s leave potential as food supplement.
Identification of water conservative tree species with high economic value around “Sendang Kalimah Toyyibah” Wiryani, Erry; Anggoro, Sutrisno; Mulyani, Sri
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 2, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.108 KB) | DOI: 10.14710/bioma.19.2.104-118

Abstract

Spring conservation require serious concern on the economic advantages for the society. Without economic advantages achieved from the conservation activities, the conservation programme should face intense conflict of land utilization. Plantation of economically valuable conservative plant species is one of the proposed solution to overcome the problem. This research aimed to identify the economic value of conservative plant species found in “Sendang Kalimah Toyyibah” surrounding. Research was conducted through field observation involving 4 line transects and 4 square transects at each line with transect size of 20 m x 20 m. Plant identification was conducted for tree strata. Data analysis was including diversity, evenness and importance index of respective plants. Economic valuation was conducted through literature study. The result showed there were 28 plants species availabile in “Sendang Kalimah Toyyibah” surrounding. Among the plant species 22 of the had been identified to provide conservative function, while 6 of them weren’t including Banana, Papaya, Melinjo, Pangi, Longan and Stink Bean. Instead of providing conservative function, most plants also provide economic advantages including wood, fruit, flower, bud, leaf, fibre, sugar, peel and bean products while only 3 of them were not identified including Banyan, Manila Tamarind and Amboyna Wood. Plantation of economically valuable conservative plant species is recommended to support the conservation of the spring as well as to provide economic advantage for the society. Keywords: conservation, economic, plant, “Sendang Kalimah Toyyibah”, spring
Pengaruh Pepton dan Waktu Inkubasi terhadap Produksi Inulinase oleh Pichia alni DUCC-W4 Berbahan Dasar Tepung Umbi Dahlia (Dahlia variabilis Willd.) Wijanarka, Wijanarka; Rukmi, MG Isworo; Sutrisna, Lynda -
Bioma : Berkala Ilmiah Biologi Vol. 09, No. 2, Tahun 2007
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.9.2.52-57

Abstract

Sumber pemanis alami alternatif yang aman bagi kesehatan dapat diproduksi dari inulin dalam umbi dahlia (Dahlia variabilis Willd.) dan dapat dihidrolisis dengan inulinase dari Pichia alni DUCC-W4. Peningkatan produksi inulinase dapat dilakukan dengan menambahkan sumber nitrogen organik berupa pepton ke dalam medium. Hasil penelitian menunjukkan aktivitas inulinase tertinggi sebesar 1,237 IU/mL (P2T3), aktivitas invertase tertinggi sebesar 1,568 IU/mL (P2T3) dan aktivitas katalitik inulinase sebesar 0,824 IU/mL (P2T2) diperoleh melalui rasio S/I dengan membandingkan aktivitas invertase (S) dan aktivitas inulinase (I). Kesimpulan dari penelitian ini adalah penambahan pepton dengan berbagai konsentrasi (0%; 0,5% dan 1%) dan lama waktu inkubasi (12 jam, 18 jam dan 24 jam) tidak meningkatkan produksi inulinase Pichia alni DUCC-W4.
Pengaruh Perendaman Akar Bibit Bayam Merah (Alternanthera amoena Voss.) dalam Larutan Na2CuEDTA terhadap Pertumbuhan dan Kandungan Antosianin Aini, Nabilah; Nurchayati, Yulita; Suedy, Sri Widodo Agung
Bioma : Berkala Ilmiah Biologi Vol. 20, No 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.173 KB) | DOI: 10.14710/bioma.20.2.123-132

Abstract

Red spinach is one of plants containing anthocyanin pigment which has various functions as food and beverage dye, and play a role in the field of health. Anthocyanin biosynthesis in plants is influenced by environmental factors, one of them are nutrients. One of the nutrients needed by plants and part of the anthocyanin biosynthesis pathway is copper (Cu). Cu can be added in the form of a fertilizer such as Na2CuEDTA. This research aims to study the effect of soaking the red spinach in Na2CuEDTA solution on growth and anthocyanin content, and knowing the optimal Na2CuEDTA concentration for growth and anthocyanin content of red spinach. This research conduct to Complete Random Design of single factor pattern with 5 treatments and 5 replications which are aquades, nutrient solution, nutrient solution+Na2CuEDTA 5 ppm, nutrient solution+Na2CuEDTA 10 ppm, nutrient solution+Na2CuEDTA 20 ppm. Sprouts red spinach that already has 4 leaves soaked in the treatment solution for 10 days, then the plants moved into a soil medium inside the pot for 20 days. The analyzing data which is used is ANOVA continuing with further testing of Duncan Multiple Range Test (DMRT) at the 95% significance level. The result of this research show that soaking of red spinach in Na2CuEDTA solution did not show any significant effect on growth. The growth of red spinach plant is more influenced by nutrient solution while the anthocyanin contents is influenced by nutrients in soil medium. Key words : Red Spinach, Copper, Anthocyanin, Na2CuEDTA

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