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AL-HAYAT: Journal of Biology and Applied Biology

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Published by Universitas Islam Negeri Walisongo Semarang

ISSN : 26543702 EISSN : 26220725 DOI : -

Core Subject : Science,

Biochemistry, Genetics & Molecular Biology

AL-HAYAT: Journal of Biology and Applied Biology an international journal focuses on biological science, biotechnology, bioremidiation, environmental biology, and biological education. It is intended to communicate original research and article review in current issues on the subject. The subject covers field studies and literary with various perspectives, both interdiscipline and multidiscipline, i.e. ecology, technology, education and much more. This journal is published by Fakultas Sains dan Teknologi, Universitas Islam Negeri (UIN) Walisongo Semarang Indonesia, and managed to be issued twice in every volume. We warmly welcome contributions from scholars and researchers of many disciplines.

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Articles 20 Documents

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Search results for , issue "Vol. 7 No. 2 (2024)" : 20 Documents clear

Antioxidant Activity Of The Peel Citrus sinensis. L On The Histological Features Of Second Degree Burned Mus musculus Samsudin, Rinza Rahmawati; Arimurti, Anindita Riesti Retno; Puspitasari, Renny Novi
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.17580

Burns are damage to the tissue that does not only occur on the surface of the skin, but can occur under the skin. Globally, burns are the fourth most common type of injury, after traffic accidents, falls and physical abuse. The cost of treating burns is relatively expensive according to the area of the burn, the larger the area of the burn, the higher the cost of treatment. Research for the treatment of burns using herbal ingredients has begun to be carried out by many researchers. One of the typical Indonesian herbal plant ingredients is the Pacitan orange (Citrus sinensis) L Osbeck. This type of research is an experimental research with the aim of looking at the formation of epithelial cells, fibroblasts, and collagen which are formed after being given Pacitan orange peel extract (Citrus sinensis) treatment. The sample of this study used 20 white rats which were divided into 4 groups, namely Group 1 (K1) the burn group without treatment, Group 2 (K2) the burn rats with bioplacenton treatment, Group 3 (K3) the burn rats with NaCl treatment 0.9%, Group 4 (K4) burnt rats treated with extra 100% Pacitan orange peel. From the research results obtained, it can be concluded that the administration of Pacitan Orange Peel extract is proven to accelerate the healing process of second degree burns on the skin of white rats viewed microscopically, namely from increased collagen production, epithelial thickness, and fibroblasts.Keywords: Pacitan orange peel extract, Burns, Mus musculus, histopathology

Diversity of Bactrocera spp. in Different Habitat Types in Citrus Orchards at Malang Regency Rahmawati, Yunita Fera; Leksono, Amin Setyo; Gama, Zulfaidah Penata; Rizali, Akhmad
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20834

Fruit flies are important pests of citrus crops. Identifying fruit fly species is very important to make the appropriate conclusions in determining controlling methods. This study was intended to establish the dominant species, diversity index, and identification of fruit fly species in Siam citrus (Citrus nobilis Lour.) orchards in Dau District, Malang Regency, Indonesia. This study was conducted in two habitat types: close and far from residential areas. Six petrogenol-treated traps were placed at each location from December 2022 to February 2023, and every two weeks, the fruit flies caught in the traps were counted as part of the survey. The results showed that three species, namely B. dorsalis, B. carambolae, and B. umbrosa, were found in locations close to residential areas, while B. umbrosa was not found in locations far from residential areas. The dominant species was B. dorsalis in both locations. The diversity index of Bactrocera spp. in orchards close to residential areas was 1.14 (moderate), and Spearman's analysis (0.913) showed a more substantial influence than in orchards far from residential areas. Climatic factors of air humidity, air pressure, and host availability play an essential role in the presence of fruit flies.

Detection of NFKB1 Gene in Patients with Type 2 Diabetes Mellitus and Non Diabetics Using Polymerase Chain Reaction Method Cahyaningtyas, Priska Putri; Sayekti, Fitria Diniah Janah
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20856

A metabolic condition called type 2 diabetes mellitus is characterized by elevated blood sugar levels because of reduced insulin release by β pancreatic cells. The primary form of NF-kB is Nuclear Factor Kappa Beta subunit-1 (NFKB1), which is a gene that encodes the DNA binding protein (p50). The NFKB1 gene contributes to the oxidative stress and mild inflammatory processes that might exacerbate diabetes. The enzymatic procedure known as Polymerase Chain Reaction (PCR), multiplies a nucleotide sequence in order to identify if type 2 diabetes mellitus or non-diabetic has the NFKB1 gene. The purpose of this study was to detect the presence of the NFKB1 gene in type 2 diabetes mellitus and non-diabetes. The study used a descriptive research method using a purposive sampling technique conducted at the molecular biology laboratory of the Sekolah Tinggi Ilmu Kesehatan Nasional. The respondents in this study were 9 patients with type 2 diabetes mellitus who were prolanes of Puskesmas Wonosari I Klaten and 9 non-diabetic patients in PKK Kadilangu RT 2/RW 1, Baki, Sukoharjo. From the results of the study, it can be seen that the NFKB1 gene was detected after electrophoresis and visualized at 176 bp (base pair). The qualitative presence of the NFKB1 gene in DNA is still detectable, but the level of gene expression in the soil of transcription and translation is not yet known.

Appropriate Concentration of Curcumin as a Growth Factor in Neural Stem Cells Novianti, Titta; Kurniawan, Muhamad Panji Januarsyah; Nainggolan, Ita M
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20862

The growth of Neural Stem Cell (NSC) in adult organisms is limited. Therefore, growth factors are needed to stimulate NSC cell proliferation and differentiation. Herbal Curcumin may be a growth factor. We promoted the growth of Cryopreserved Rat Cortical NSC cells with Curcumin (0.1 µM; 0.5 µM; 1 µM; 2 µM), DMSO, and synthetic growth factors (bFGF, TGF, and heparin). We analyzed the proliferation ability of NSCs by WST-1 assay, cell morphology, and expression of NCS cell marker genes (Nestin, MAP, and Sox2). Morphological analysis showed that cells reproduced optimally at 0.5 µM. The one-way ANOVA and Tukey's posthoc test on the WST-1 test showed significant differences between 0.5 µMCurcumin and other treatment groups. Sox2, MAP-2, and Nestin gene expression peaked at 0.5 µM. The appropriate concentration of Curcumin to stimulate NSC proliferation is 0.5 µM. Herbal extract curcumin has the same effect as commercial growth factors and can substitute synthetic growth factors. Curcumin acts as a growth factor that stimulates the proliferation of mouse NSCs.

Composition and Distribution of Invasive Species in The Peson Subah I Nature Reserve, Batang District, Central Java santoso, budi; Kurniayu, Tiara Putri
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20963

Research has been carried out regarding the composition of invasive plants in the Peson Subah I Nature Reserve (PSINR), a conservation area located at Kuripan Village, Subah District, Batang Regency Central Java. The research, which was carried out from 17 to 20 April 2023, aims to identify invasive plants in PSINR and mapping their spatial distribution. This research used a double plot method which was determined purposively with a sampling intensity of 10%. The analysis method used is quantitative descriptive based on the parameters of the vegetation analysis results. Based on the results of observations, 80 plant species were found, of which 53% or 43 species were invasive species. The highest Importance value Index for the invasive species at each tree level is Acacia (Acacia auriculiformis) 66.44, at the pole level it is Klampis (Flacourtia indica) with an Importance value index of 91.46, at the sapling level it is Lampeni (Ardisia elliptica) and at the seedling level the invasive species Lampeni (Ardisia elliptica) 55.16. The plant diversity index in PSINR at each growth level is in the medium category, while the evenness index is in the high to medium category.

Dimensional Stability Test of Mahogany Wood by Heat Treatment Kusumawardhani, Dina Tiara
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.22225

Mahogany wood (Swietenia macrophylla) is one of Indonesia's fast-growing commercial tree species. Its wood is widely used for construction because of its great potential in the timber industry. Mahogany wood has low dimensional stability and high hygroscopic properties. This study aims to improve the quality of mahogany wood in terms of dimensional stability with the heat treatment method. Samples of mahogany wood were heat treated with three-time levels, namely control, 3 hours, and 6 hours at 180 °C. The results showed that heat treatment at 180 °C for 6 hours had the best results for maintaining wood dimensions with a low increase in water content, increased specific gravity, low absorption ability, minor swelling of wood in three directions of wood, and the value of ASE is also common than 3 hours of treatment.

Differences in the Number and Karyotypes of Chromosomes of the Anura Order in Indonesia Khairati, Ikmanisa; Razak, Abdul
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.23808

The diversity of an organism can be studied down to the cellular level. Cytological data related to certain organisms obtained can be used as information to classify these organisms. One of them is by examining the number and karyotypes owned by organisms. A karyotype is a chromosomal phenotype that includes its structural description. The purpose of this study is to see the difference in the number and karyotype of chromosomes in species belonging to the order Anura in Indonesia. The method used is a literature study of various studies related to chromosomes of species belonging to the order Anura. Based on the results of the analysis, there are differences in the number and karyotype of several species classified in the order Anura. The species of the order Anura studied in this study are Rana rufipes, Rana parvaccola, Huia sumatrana, Fejervarya limnocharis, Limnonectes cf. grunniens, Limnonectes cf. modestus, Fejervarya cancrivora, Limnonectes blythii, Polypedates celebensis, Polypedates leucomystax, Microhyla pulchra, which belongs to the families Ranidae, Dicroglossidae, Rhacophoridae, and Microhylidae. The variation in the number of diploid chromosomes of the organism studied was in the range of 22-26 chromosomes. In addition to the number of chromosomes, there are also differences in chromosomal karyotypes in each organism

The Diversity of Terrestrial Ferns in the Customary Forest of Depati Karo Jayo Tuo, Jambi Nazila, Jumiatun; Husnudin, Uni Baroroh; Ihsan, Mahya; Hariyadi, Bambang; Maritsa, Hasna Ul
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.23967

The Depati Karo Jayo Tuo Customary Forest represents a rich ecosystem of Sumatran rainforest biodiversity. Observations reveal dense canopy cover and various terrestrial fern species, yet information regarding the diversity of terrestrial ferns remains limited. Exploratory research is essential to uncover the biodiversity of terrestrial ferns in this area, including their conservation to support effective conservation strategies, protect species and ecosystems. This study aims to identify the diversity of terrestrial fern species and evaluate their conservation status. The research employs the cruise method in the trekking path of the forest. Environmental factors measured include soil temperature, soil moisture, pH, air temperature, humidity, and light intensity. Species identification is based on the identification keys of ferns. Conservation status was analyzed based on IUCN and CITES. The study found 49 species divided into Filicinae class (45 species, 24 genera, 18 families, 5 orders) and Lycopodiinae (4 species, 1 genus, 1 family, 1 order). According to the IUCN, 32 species are categorized as Not Evaluated, 2 species as Least Concern (LC), and 15 species remain uncategorized. CITES data revealed that one species is listed under Appendix II, so it is important to protect biodiversity in the Depati Karo Jayo Tuo Customary Forest.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20834

Fruit flies are important pests of citrus crops. Identifying fruit fly species is very important to make the appropriate conclusions in determining controlling methods. This study was intended to establish the dominant species, diversity index, and identification of fruit fly species in Siam citrus (Citrus nobilis Lour.) orchards in situs gacor777 Dau District, Malang Regency, Indonesia. This study was conducted in two habitat types: close and far from residential areas. Six petrogenol-treated traps were placed at each location from December 2022 to February 2023, and every two weeks, the fruit flies caught in the traps were counted as part of the survey. The results showed that three species, namely B. dorsalis, B. carambolae, and B. umbrosa, were found in locations close to residential areas, while B. umbrosa was not found in locations far from residential areas. The dominant species was B. dorsalis in both locations. The diversity index of Bactrocera spp. in orchards close to residential areas was 1.14 (moderate), and Spearman's analysis (0.913) showed a more substantial influence than in orchards far from residential areas. Climatic factors of air humidity, air pressure, and host availability play an essential role in the presence of fruit flies.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20862

The growth of Neural Stem Cell (NSC) in adult organisms is limited. Therefore, growth factors are needed to stimulate NSC cell proliferation and differentiation. Herbal Curcumin may be a growth factor. We promoted the growth of Cryopreserved Rat Cortical NSC cells with Curcumin (0.1 µM; 0.5 µM; 1 µM; 2 µM), DMSO, and synthetic growth factors (bFGF, TGF, and heparin). We analyzed the proliferation ability of NSCs by WST-1 assay, cell morphology, and expression of NCS cell marker genes (Nestin, MAP, and Sox2). Morphological analysis showed that cells reproduced big77 optimally at 0.5 µM. The one-way ANOVA and Tukey's posthoc test on the WST-1 test showed significant differences between 0.5 µMCurcumin and other treatment groups. Sox2, MAP-2, and Nestin gene expression peaked at 0.5 µM. The appropriate concentration of Curcumin to stimulate NSC proliferation is 0.5 µM. Herbal extract curcumin has the same effect as commercial growth factors and can substitute synthetic growth factors. Curcumin acts as a growth factor that stimulates the proliferation of mouse NSCs.

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