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AL-HAYAT: Journal of Biology and Applied Biology
Published by Universitas Islam Negeri Walisongo Semarang
ISSN : 26543702     EISSN : 26220725     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
AL-HAYAT: Journal of Biology and Applied Biology an international journal focuses on biological science, biotechnology, bioremidiation, environmental biology, and biological education. It is intended to communicate original research and article review in current issues on the subject. The subject covers field studies and literary with various perspectives, both interdiscipline and multidiscipline, i.e. ecology, technology, education and much more. This journal is published by Fakultas Sains dan Teknologi, Universitas Islam Negeri (UIN) Walisongo Semarang Indonesia, and managed to be issued twice in every volume. We warmly welcome contributions from scholars and researchers of many disciplines.
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol. 8 No. 2 (2025)" : 8 Documents clear
Isolation and Identification of Endophytic Fungi from leaves and petioles of Salam Plants (Syzygium polyanthum Wight) to Look for Antibiotic-Producing Fungi Yudiarti, Turrini; Tri Agus Sartono; Endang Widiastuti; Hanny Indrat Wahyuni; Sugiharto; Ika Agusetyaningsih
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i2.25602

Abstract

Synthetic antibiotics have been used to stimulate livestock growth. Because of the negative impact of using these material, it is necessary to find its substitute. One of the producer of natural antibiotics is microorganisms such as fungus. Endophytic fungi are one kinds of fungi that live in plant tissues and able to form colonies without endangering the host and can produce secondary metabolites such as antibiotic. This study is preliminary research that the ending of the purpuse is to find the endophytic fungi which producing antibiotic. This first aims of the study is to psolation and identification of the endophytic fungi found on leaves and petioles of Salam (Syzygium polyanthum Wight). The materials used are leaves and petioles of Salam which are obtained from traditional markets. The method was the isolation by direct planting used spread methods, then continue to identification. The results was the isolation of endophytic fungi from the Salam plant parts obtained as follows from leaves got five isolates and from petiole are seven isolates. Identification of all isolates found as follows from leaves are Mycelia sterilia, Acremonium sp and Fusarium sp and from petioles were Mycelia sterilia , Fusarium sp, Aspergillus sp and unidentified fungi. Keywords: endophytic fungi, antibiotics, petiols, leaves, Syzygium polyanthum
Biological Control Agent of Spodoptera frugiperda Using Bacillus thuringiensis Bacteria Indriyani, Indriyani; Handayani, Kusuma; Firmansyah, Alvian; Pratami, Gina Dania
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i2.26475

Abstract

The main obstacle in the development of food crop production and horticulture is the attack of S. frugiperda larvae. Pest control using chemical insecticides has a long-term negative impact. The biological control of Spodoptera frugiperda larvae is accomplished by utilizing B. thuringiensis bacteria. This study aims to determine the best B. thuringiensis isolate for killing S. frugiperda larvae, to identify the most effective concentration of B. thuringiensis suspension, and to ascertain the mortality rate of S. frugiperda larvae. The design of this study was a randomized block design (RBD) with two factors. The first factor was the type of B. thuringiensis isolate, consisting of four isolates. Larval mortality data were analyzed using Analysis of Variance (ANOVA) and further tested with the Duncan test. The results obtained showed the highest average mortality of S. frugiperda larvae using isolates Bt3BP14 and Bt4TSR6. These two isolates had the highest average mortality on the third day. Bt3BP14 and Bt4TSR6 isolates have high potential in controlling S. frugiperda larvae, with an average mortality proportion of 86,67% and 66,67% observed over three days. The best doses of B. thuringiensis suspension for killing S. frugiperda larvae were 15 ml and 20 ml. It can be concluded that B. thuringiensis isolates can be used as natural biological control agents against S. frugiperda larvae that attack food crops and horticulture.
Plants sold in traditional marketplace in West Java highland: an ethnobotanical analysis Fitriah, Lia; Mulyanto, Dede
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i2.28426

Abstract

Traditional markets are crucial for sustaining plant diversity, local food systems, and cultural heritage. They also function as learning spaces for the diversity and cultural connections of food plants. This study documents the diversity and utilization of plant species traded in a traditional marketplace in West Java’s highland, Indonesia. Data were collected through stall surveys, observations, and semi-structured interviews with 38 traders. A total of 96 species from 31 families were recorded. Fabaceae and Solanaceae were the most represented families (11 species each). Vegetables were the largest category of use (47%); fruits (53%) and leaves (25%) were the most frequently used parts. Most (83%) of the plants sold were exotic, with only 21% being native species. Eight edible wild plant species, including Centella asiatica and Solanum nigrum, remain an essential part of the traditional diet. Women dominate the vegetable trade (64.51%); most vendors reported having 5–20 years of experience. These findings highlight that traditional markets play a crucial role in preserving and serving as learning spaces for plant diversity, local food systems, and cultural heritage.
Isolation and Molecular Identification of Cadmium-Resistant Bacteria from Paddy Soil in the Tallo Watershed, Makassar Nurhikmayani, Risky; Dewi, Aghis Sukma
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i2.28874

Abstract

Cadmium contamination of paddy soils is a global concern because Cd can accumulate in rice through nutrient absorption, posing a food safety risk. More than a quarter of the Tallo watershed area, Makassar is a rice field that is vulnerable to pollution due to industrial activities and water runoff from urban areas. This study aims to isolate and identify Cd-resistant bacteria from paddy field soils in the Tallo watershed to find potential isolates that have the potential for bioremediation. The soil samples collected were from the rhizosphere of rice fields in the Tallo watershed, which were varying distances from the Tallo Main River. Bacterial isolation used nutrient agar media, followed by Cd resistance testing using Nutrient Agar-CdCl₂ media with increasing concentrations from 10 mg L-1 to 110 mg L-1. Molecular identification was carried out on the most Cd-tolerant isolates using the 16S rRNA marker gene with primers 27F and 1492R. From the isolation, 22 isolates were obtained, with 19 isolates resistant to CdCl2 10 mg L-1; Only five isolates could continue growing at a concentration of 100 mg L-1, and only one isolate could grow up to a concentration of 110 mg L-1; the isolate was L1(4). The isolate L1(4) exhibited the best growth and was identified as Aeromonas veronii through 16S rRNA gene sequencing. These findings highlight Aeromonas veronii as a promising candidate for the bioremediation of Cd-contaminated soils in the Tallo watershed
Developmental Anatomy of Anther in the Red Dragon Fruit (Hylocereus polyrhizus Britton & Rose) Utaminingsih, Utaminingsih
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i2.28955

Abstract

The developmental anatomy of the anther in Hylocereus polyrhizus (red dragon fruit) was examined to elucidate the sequential processes of microsporogenesis and microgametogenesis that underlie pollen formation. Flower buds were sampled at five developmental stages (1-5) and analyzed histologically using paraffin embedding and safranin staining. Anther length increased progressively by 96% from stages 1 to 4 and plateaued before anthesis, indicating growth cessation before pollen maturation. The anther wall followed a dicotyledonous pattern, comprising the epidermis, endothecium, middle layer, and tapetum. Early stages featured archesporial differentiation into primary parietal and sporogenous cells, followed by the formation of secondary parietal and sporogenous layers. The endothecium developed fibrous thickenings, while the binucleate, secretory tapetum provided nutrients for developing microspores and degenerated at maturity. Microsporogenesis proceeded from microspore mother cells to tetrads and solitary microspores, culminating in tricellular pollen grains at anthesis. These findings demonstrate that anther development in H. polyrhizus conforms to the typical dicotyledonous type, with characteristic features such as a secretory tapetum and fibrous endothecium. The results provide essential anatomical insight to support breeding programs, artificial pollination strategies, and reproductive biology studies of this economically valuable cactus species.
The Protective Effect Of Rambutan Honey On Macroscopic And Microscopic Examination Of Pancreas Rattus norvegicus Induced By Traditional Alcoholic Beverages Ciu Sayekti, Fitria Diniah Janah; Kirana Putri , Pramesti
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i2.26971

Abstract

The traditional ciu alcoholic beverages is a drink made from distilled sap and sticky tape. Ciu is a type of drink that has an alcohol content of 25%. Alcoholic beverages will be toxic if consumed in excess. The pancreas is one of the organs that undergoes a process of damage when toxicmaterials or metabolites accumulate in the organ. Rambutan honey is a natural ingredient that contains flavonoid compounds. Flavonoids act as antioxidants which can reduce damage to pancreatic cells due to toxic substances. The aim of this study was to determine the protective effect of rambutan honey on the macroscopic and microscopic examination of white rats (Rattus norvegicus) induced by traditional ciu alcohol. This research is experimental with 5 treatment groups including 1 control normal, 1 control negative and 4 ciu and honey treatments. Pancreatic tissue was observed macroscopically for color and texture. Microscopic observations were observed in the form of degeneration and necrosis. The microscopic results showed that there was a color difference between the control group and the alcohol-induced honey treatment group. The results of microscopic observations were known to show degeneration and necrosis in the positive group and the treatment group. The results of the study based on the ANOVA test obtained <0.05. Based on the Anova test and Duncan test it can be concluded that rambutan honey has a protective effect on the pancreatic histopathology of white rats (Rattus norvegicus) in the form of normal cells and degenerated cells in groups K2 and K3
Starter Culture Modulates Microbial Diversity During Wine-Coffee Fermentation: A DGGE-Based Molecular Study Nasir, Fitri Handayani; Astuti, Dea Indriani; Taufik, Intan; Fitriagustiani; Ikramullah, Muh. Chaeril; Putri, Nurul Febriani; Pasmawati
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i2.28269

Abstract

Wine-flavored coffee is a unique post-harvest product characterized by fruity, acidic, and winey notes, highly favored by Indonesian consumers and possessing strong potential in the global market. However, maintaining consistency in flavor and quality remains a challenge due to the variability of natural fermentation. This study aimed to evaluate the impact of yeast and bacterial starter culture inoculation on microbial community dynamics during wine-coffee fermentation, using a molecular approach based on PCR-DGGE. DGGE analysis revealed that natural fermentation without inoculation involved diverse populations of bacteria, yeasts, and filamentous fungi, with eukaryotic microbes dominating from the early stages. Sequencing identified prevalent yeast genera including Pichia, Torulaspora, Hanseniaspora, Saccharomyces, and Candida, as well as Aspergillus among filamentous fungi. Bacterial communities were dominated by lactic acid bacteria and members of Lactobacillus, Klebsiella, Enterobacter, Pantoea, and Bacillus. In contrast, controlled fermentation with inoculated starter cultures (Pichia kudriavsevii and Klebsiella sp.) showed a more stable microbial profile throughout the process. Shannon-Wiener diversity indices demonstrated a significant difference (p = 0.05) between natural and inoculated fermentations, with species dominance observed in the latter. Cluster analysis confirmed that starter culture inoculation significantly influenced microbial succession and community structure. These findings highlight the importance of controlled fermentation using selected microbial starters to ensure consistent microbial ecology, which in turn contributes to reproducible quality in wine-flavored coffee. The molecular profiling approach provides valuable insights for improving fermentation practices and developing reliable starter culture formulations tailored to enhance flavor consistency and product quality.
The Gel Formulation of Clitoria ternatea L. Flower Extract on Total Flavonoid Content Wardhani, Irma Yuniar; Hayya, Adieba Warda; Hilman, Khansa Melatika; Febrian, Rizki Gatot
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i2.28806

Abstract

Clitoria ternatea L. or telang flower is widely used as a natural food coloring, herbal tea and also as an additional active ingredient in pharmacological products. C. ternatea contains antioxidants are good for protecting cells from free radicals, maintaining the immune system, anti-aging and good for skin health. The aim of this research was to determine the gel formulation of C. ternatea flower extract regarding its total flavonoid content. The formulation results have gone through physical quality tests and total flavonoid tests. Physical quality test parameters include organoleptic test, homogeneity test, pH test, spreadability test, hedonic test, moisture test, and irritation test. The high extract content will increase the flavonoid content in the gel formulation, and it also make the color of the preparation more intense (purplish blue), making it less preferred by users. Therefore, from the hedonic test results, it is known that is preferred by users is with a concentration of C. ternatea condensed extract of 1%. The last formulation showed that the total flavonoid content of C. ternatea gel formulation had a total flavonoid content of 63.21 ug/g using UV-Vis spectrophotometry. This flavonoid content indicates that the gel formulation contains antioxidants are good for skin health.

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