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Formulasi Krim Tipe A/M dari Ekstrak Rimpang Cyperus rotundus L. Untuk Pengobatan Nyeri Sendi Yenti, Revi; Rahim, Farida; Firmansyah, Firmansyah; Syayuni, Aziza
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 8, No 2 (2016)
Publisher : Indonesian Research Gateway

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The purpose of this study were to formulate and determined the effect of the cream type W/O ethanolic extract of the rhizome of teki (Cyperus rotundus L) as join paint medicine. This research were uses three formulas (F1, F2,and F3) with concentration 3%, 5% and 7% of ethanol extract. The formulas were evaluated for their organoleptic, homogenity, cream type, pH, washability, stability of temperature, particle size distribution, skin irritation. The test of curing the join pain effect has done to the white male rats which induced by AgNO 3 1% as the pain inductor via intra articular. The parameter that we have seen is amount of sweaking in 30 minutes, an hour, 1,2,4,6 and 8 hours. The cream formula of ethanol extract of the rhizome of teki was consistant in allconcentrations. Based on the result of statistical, group V (Formula 3) was gave faster reaction in fourth time of observation than other formula.

Validasi Metode Kromatografi Cair Kinerja Tinggi Untuk Analisis Rhodamin B Dalam Saos Tomat Yantih, Novi; Aziz, Zuhelmi; Aprillia, Heidy
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 8, No 2 (2016)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Rhodamine B is a synthetic red dye which is commonly used as textile dyes and banned its use in food and beverages. The red dye is harmful to health and it can cause poisoning symptoms if it is swallowed, and it can damage the liver when it is taken long term. Although it has been banned because of its toxicity, but there is abuse of rhodamine B as a food coloring, such as in tomato sauce product. The purpose of this study is to validate the high performance liquid chromatography (HPLC) for analysis of rhodamine B in a tomato sauce. The analytical method used is HPLC with reverse phase system. The analytical method has met the requirements of method validation according to the International Conference of Harmonization with the detection and quantitation limits, 1 ppm and 3 ppm respectively. Based on the results of the qualitative analysis of rhodamine B in tomato sauce samples, one of the three samples of tomato sauce that is not listed in NA-DFC (The National Agency of Drug and Food Control) showed positive result, it was shown with the same retention time between samples and standards (5.5 minutes). Average level of rhodamine B in the sample was 25 μg/g of tomato sauce.

Validasi Metode Kromatografi Cair Kinerja Tinggi Untuk Analisis Rhodamin B Dalam Saos Tomat Yantih, Novi; Aziz, Zuhelmi; Aprillia, Heidy
Jurnal Farmasi Indonesia Vol 8, No 2 (2016)
Publisher : Jurnal Farmasi Indonesia

Rhodamine B is a synthetic red dye which is commonly used as textile dyes and banned its use in food and beverages. The red dye is harmful to health and it can cause poisoning symptoms if it is swallowed, and it can damage the liver when it is taken long term. Although it has been banned because of its toxicity, but there is abuse of rhodamine B as a food coloring, such as in tomato sauce product. The purpose of this study is to validate the high performance liquid chromatography (HPLC) for analysis of rhodamine B in a tomato sauce. The analytical method used is HPLC with reverse phase system. The analytical method has met the requirements of method validation according to the International Conference of Harmonization with the detection and quantitation limits, 1 ppm and 3 ppm respectively. Based on the results of the qualitative analysis of rhodamine B in tomato sauce samples, one of the three samples of tomato sauce that is not listed in NA-DFC (The National Agency of Drug and Food Control) showed positive result, it was shown with the same retention time between samples and standards (5.5 minutes). Average level of rhodamine B in the sample was 25 Î¼g/g of tomato sauce.

Aktivitas Antibakteri dan Antioksidan Daun Pecut Kuda (Stachytarpheta jamaicensis L.) Secara Invitro Kumala, Shirly; Bekti, Novi Dyah Purnama
Jurnal Farmasi Indonesia Vol 8, No 2 (2016)
Publisher : Jurnal Farmasi Indonesia

Pecut Kuda leave (Stachytarpheta jamaicensis L.) was one of the many medicinal plants found in Indonesia and it has been widely used by the people in Indonesian to cure infection of tractus urinarius, pharingitis, hepatitis. Although, it has been very popular traditional medicine, very little scientific data were collected until now. This study focused on preliminary studies of antibacterial and antioxidant activity of Pecut Kuda leaves. Extracts were obtained using n-hexane, ethyl acetate and methanol. The antibacterial activity test was performed using the agar diffusion method against Staphylococcus aureus and Escherichia coli. While antioxidant activity was performed by the colorimetric method using DPPH radical reduction. The results showed that n-hexane extracts did not have either antibacterial or antioxidant activity. The ethyl acetate extract had zone inhibition (DDH) between 7 to 11.67 mm against S. aureus bacteria, whereas against E. coli with DDH between 7.67 to 10.83 mm, and had IC50 value of 182.97%. The methanol extract had DDH between 8.33 to 13.67 mm against S.aureus, whereas against E. coli bacteria with DDH between 9 to 14.67 mm, and IC50 value of 14.28%. In conclusion, ethyl acetate and methanol extract of Pecut kuda leaves have antioxidant and antibacterial activity against S. aureus and E. coli bacteria.

Aktivitas Antidiabetes Ekstrak Etanol Kulit Batang Faloak (Sterculia quadrifida R.Br.) Terhadap Tikus Wistar yang Diinduksi Aloksan Diki Dongga, Irene Rambu Yety; Sunarti, Sunarti; Sunarti, Titik
Jurnal Farmasi Indonesia Vol 8, No 2 (2016)
Publisher : Jurnal Farmasi Indonesia

Diabetes mellitus is a chronic disease with hyperglycemic and oxidative stress component that can lead to various complications. The stem bark of faloak have been used traditionally to treat diabetes by local people in East Nusa Tenggara. Therefore, this research aimed to study antidiabetic activities in alloxan-induced rats of faloak ethanolic extract. The all rats were divide at random into group I : normal control; groups II : diabetic control; group III : standart (glibenclamide); group IV â?? VI : administrations group of faloak stem bark ethanol extract at a dose of 65 mg / kg, 130 mg / kg and 260 mg / kg for 14 days. Blood samples were collected from rats in duration 0 â?? 14 days for T0, T4, T7 and T14 after alloxan treatments. The results showed that ethanol extract of faloakâ??s bark dose of 260 mg/ kg on 14 days had decreased blood glucose levels 46,07%, no different glibenclamide group. The dose of 65 mg/kg and 130 mg/kg reduced glucose level respectively 26,32 % and 38,09 %.

Standarisasi Ekstrak Sarang Semut untuk Pemenuhan Mutu Ekstrak Menuju Obat Herbal Terstandar Antikanker Lindawati, Novena Yeti; Murtisiwi, Lusia
Jurnal Farmasi Indonesia Vol 8, No 2 (2016)
Publisher : Jurnal Farmasi Indonesia

Dry extract of sarang semut (Myrmecodia pendens Merr & Perry) water/n-butanol fraction is native plant from Papua is proven to be active against cancer cells. This research aims to improve the quality of extracts of sarang semut become the raw material that meets the standards of medicinal plant extracts in the fulfillment of the quality of the extracts into anticancer Standardized Herbal Medicine / Obat Herbal Terstandar (OHT). Extraction with ultrasonic methods (1:10 with methanol) and fractionation with water:butanol (1:1) as much as 1:20. Standardization and data analysis based on parameters of the Medicinal Plant Extracts (Kepmenkes RI No: 55/Menkes/SK/I/2000) and BPOM RI Regulation No. 12, Year 2014 about quality requirements of traditional medicine. Dry extract of sarang semut (water/n-butanol fraction) met the standards of raw material preparation for OHT with organoleptics shaped dried extracts, dark brown color, aromatic smell, and has a sour-bitter taste. Moisture content was 9,259 Â± 0.001 (â?¤ 10%). Total Plate Count was 5 x 103 colonies/g (< 104 colonies/g). Yeast and Mold Count was 0,5 x 101 colonies/g (< 103 colonies/g). Pathogenic bacteria test shows that MPN Coliform value was 0 colonies/g and negatively to the presence of Escherichia coli, Salmonella sp, Staphylococcus aureus, Pseudomonas aeruginosa, and Shiggella sp. The extract was not detected the presence of aflatoxin total (aflatoxin B1, B2, G1 and G2). The content of heavy metal Cd was 0,0052 Â± 0.0005 ppm (â?¤ 0.3 ppm) and heavy metals Pb was 0,9429 Â± 0,0087 ppm (â?¤ 10 ppm).

Ekstrak Daun Binahong Mencegah Kenaikan Kolesterol Darah pada Tikus yang Diberi Pakan Lemak Tinggi Rahman, Asep Abdul; Kurniati, Neng Fisheri; Sukandar, Elin Yulinah
Jurnal Farmasi Indonesia Vol 8, No 2 (2016)
Publisher : Jurnal Farmasi Indonesia

Binahong (Anredera cordifolia (Ten.) v. Steenis) leaves empirically have been used to lower cholesterol levels. In this study, preventive activity of ethanol extracts will be tested on the animals fed with a high-cholesterol chow. Hypercholesterolemic animal model established by orally administering high-cholesterol chow, pure cholesterol, cholic acid, and propiltiourasil. The test animals were divided into 5 groups, including the positive control group, ethanol extract of binahong leaves at doses of 50 mg/kg bw, 100 mg/kg bw, and 200 mg/kg bw; and simvastatin at dose of 36 mg/kg bw. Extract was given at the same time as hypercholesterol induction. Lipid profiles were measured at the time before the induction, 15 days and 29 days after induction. Index of atherogenic was also calculated. The results showed that level of cholesterol and LDL of extract binahong at dose of 50 mg/kg (109.17Â±26.60 mg/dL and 55.78Â±23.92 mg/dL, respectively) had significant difference compared to a positive control group (p < 0.05). Furthermore, binahong extract at dose of 50 mg/kg bb had the lowest atherogenic index (0,68Â±0,27) and aorta thickening (150.60Â±41.72 Î¼m) compared to other extracts at day 15 of induction. In conclusion, extract binahong at dose of 50 mg/kg bw rat gives the best effect to prevent the increase of cholesterol and LDL level on wistar male rats fed with a high-cholesterol chow.

Isolasi dan Skrining Aktivitas Antimikroba Jamur Laut pada Alga Kappaphycus alvarezii dari Kabupaten Takalar Sulawesi selatan Fitriana, Fitriana; Julianti, Elin; Wibowo, Marlia Singgih
Jurnal Farmasi Indonesia Vol 8, No 2 (2016)
Publisher : Jurnal Farmasi Indonesia

Marine microorganisms, especially actinomycetes bacteria and fungi produce a variety of secondary metabolites that biologically active and have a unique structure. This study aims to isolate some strains of marine fungi that grow on marine algae as a potential source of antimicrobial agent. Marine fungal strains isolated from the marine algae with antimicrobial activity screening is done by using the disc diffusion method. In this study, it was obtained 18 strains of fungal isolates. The screening results that give best antimicrobial activity shown by the activity of the extract liquid culture of fungal isolate strain AKT.C6 are 10.0 mm against the bacteria Escherichia coli and 10.8 mm against Bacillus subtilis. Mycelium extract of fungal isolates strain AKT.C4 is 16.9 mm against the bacteria Escherichia coli and 17.6 mm against Bacillus subtilis, while the liquid culture extract and mycelium extract did not show any activity against Candida albicans. The isolation of fungi from Kappaphycus alvarezii algaee has potential as an antibacterial agent.

Efektivitas Enhancer Natrium Lauril Sulfat Dalam Patch Topikal Antiinflamasi Ekstrak Etanol Kencur (Kaemferia Galanga L.) Terhadap Jumlah Neutrofil dan Makrofag Pada Mencit Fatmawati, Desy; Dermayati, Cyntia Zain; Hamid, Iwan Sahrial; Hendriati, Lucia
Jurnal Farmasi Indonesia Vol 8, No 2 (2016)
Publisher : Jurnal Farmasi Indonesia

Ethanol extract Kencur (Kaempferia galanga L.) has been proven to have antiinflammatory effect as well as to decrease the side effect of NSAIDs drug use, to develop the formulation of ethanol extract of kencur, then made in the dosage form of topical patch. Topical patch preparations are expected to avoid effects on the gastrointestinal tract, first- pass metabolism, and facilitate the use of the patient thereby increasing patient compliance. In the patch formulation, the most important things to note is the penetration enhancer/ enhancer, and enhancer used in this study is sodium lauryl sulphate. This study aims to determine the effectiveness of sodium lauryl sulphate in the preparation of a topical patch of kencur extract as anti inflammatory on the number of neutrophils in the blood. The research method uses canrrageenan as an inflammation inductor and neutrophil count observation was conducted using blood smears, then was observed on the first, third and seventh. Group of treatment which was given sodium diclofenac gel as positive control, group of treatment which was without enhancer as the 1st group, groups of treatment which were givenenhancer 1%, 3%, and 5% as the 2nd, 3rd and 4th group. The result was analyzed by one way ANOVA, followed by Post Hoc Duncan. The experimental results prove that there was a significant difference between the number of neutrophils and macrophages in the negative control group who were not given enhancer and the treatment group were given enhancer sodium lauryl sulphate values ( p < 0.05 ). As well as the group treated with the addition of enhancer sodium lauryl sulfate showed a greater amount of decline compared to the treatment group that contains only ethanol extract kencur.

Evaluasi Efek Teratogen Ekstrak Terpurifikasi yang Diisolasi dari Fraksi Etil Asetat Daun Surian (Toona sureni (Blume) Merr) Suharti, Suharti; Yosmar, Rahmi; Fitriani, Ade; Almahdy, Almahdy
Jurnal Farmasi Indonesia Vol 8, No 2 (2016)
Publisher : Jurnal Farmasi Indonesia

Evaluation of teratogenic effects of purified extract isolated fromethyl acetate fractionation of surian leaves (Toona sureni (Blume) Merr.) onwhite female fetus mice has been carried out. The extract was given to miceorally in solution at doses of 5, 10, and 20 mg/kg BW on 6th until 15th day ofpregnancy. On 18th day of pregnancy, laparatomy was performed, then twoâ?? third of fetuses were immersed in alizarin red solution and the remainingin Bouinâ??s solution. The analyzed parameters were the weight of pregnantmice, the number of fetuses, the weight of fetuses, skeletal malformation andmorphology. The result showed that malformations were not shown in dose of 5mg/kg BW, but in dose of 10 mg/kg BW from one mice found one fetus causedlate growth and death. While on dose 20 mg/kg BW, one mice was not death.

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Journal Mail Official
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Kota adm. jakarta barat,

Dki jakarta

INDONESIA