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INDONESIA
YARSI Medical Journal
Published by Universitas Yarsi
ISSN : 08541159     EISSN : 24609382     DOI : -
Core Subject : Health,
Medicine & Pharmacology
Arjuna Subject : -
Articles 232 Documents
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THE PRESENCE OF PROTEIN A ON THE SURFACE OF STAPHYLOCOCCUS AUREUS BACTERIAL CELLS USING SERUM SOFT AGAR TECHNIQUES Djannatun, Titiek
Jurnal Kedokteran YARSI Vol 24, No 3 (2016): SEPTEMBER - DESEMBER 2016
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v24i3.263

Abstract

Protein A is a specific proteins on the surface of some  Staphylococcus aureus (S. aureus) strains. Staphylococcus bacteria which have protein A has widely used in the biomedical. Aims of this study  to find a simple method of detecting the presence of S. aureus protein A. 15 S. aureus isolates from field cases, S. aureus cowan I as positive control, Staphylococcus epidermidis dan Micrococcus sp as negatif control were used.  Bacteria was inoculated into 10 ml Todd Hewith Broth medium, Soft Agar (SA) and into 10 ml of serum soft-agar (SSA), agitated using a vortex and incubated at 37oC for 18-24 h. Form of colonies that grew was observed and categorized as compact and diffuse colonies. The colonies that suspected contain protein A conducted confirmation tests by Dot Blot. 7  isolates showed the change of colony formation from diffuse to compact in SSA (SA added rabbit or chicken serum), and 8 isolates remain diffuse. Dot Blot test positive. Protein A has the ability to bind the Fc fraction of IgG but not the IgY from birds. The binding of S. aureus and IgG could be demonstrated by the change of colony growth from diffuse before the addition of serum containing IgG in SA to compact after the presence of serum. Protein A on the bacterial cell surface bind the Fc-IgG caused steric hinderance of bacteria and expressed as compact colony formation in SA. SSA using mammalian sera can be used to discriminate the bacterial strains with or without protein A.
EFEK KURKUMIN DAN PENTAGAMAVUNON-0 TERHADAP VIABILITAS KULTUR SEL LUTEAL Purwaningsih, Endang
Jurnal Kedokteran YARSI Vol 17, No 1 (2009): JANUARI - APRIL 2009
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.347 KB) | DOI: 10.33476/jky.v17i1.196

Abstract

The purpose of this study was to investigate the effect of curcumin and pentagamavunonn-0 (PGV-0) on the viability of cultured luteal cells (percent of viable cells). The cultured luteal cells were obtained from corpus luteum of induced 12 immature Sprague Dawley rats by a single 10 IU Pregnat Mare?s Serum Gonadotropin. Subject in this study was 4 days old corpus luteum which was cultured in Minimal Essential Medium (MEM) containing 10% Fetal Bovine Serum. This experiment consisted of 12 groups, e.g. one group as control (solvent), three groups as control with LH and or PGF2 stimulation, and eight groups treated with 100 ?M curcumin and PGV-0 in three replicates. Evaluation was conducted after 24 hours of treatment by observation of viable cells with MTT method. The result showed that the viability of cultured luteal cells with curcumin or PGV-0 only was not significantly different compared to control goups. However, curcumin and PGV-0 on cultured luteal cells stimulated by LH and or PGF2 showed significant differences compared to control or LH and PGF2 only.
JENIS BAKTERI DAN JAMUR KONTAMINAN UDARA DI RUANG PERAWATAN SUB BAGIAN PENYAKIT DALAM RUMAH SAKIT UMUM DAERAH BANJARBARU Yulia Budiarti, Lia; Noormuthmainah, Noormuthmainah; Rahmiati, Rahmiati
Jurnal Kedokteran YARSI Vol 15, No 1 (2007): JANUARI - APRIL 2007
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.807 KB) | DOI: 10.33476/jky.v15i1.1005

Abstract

Nosocomial infection is one of the major health problem in the world. One of the way for transmitting the causative microorganism of nosocomial infection is airborne. This research had been done to know kinds of air contaminating bacteria in sub departement of internal disease science of General Hospital Banjarbaru. This research was laboratory descriptive using ?open plate? method. The results of research described that there were five kinds of bacteria, they were Staphylococcus epidermidis 38,07%, Escherichia coli 27,52%, Streptococcus ? haemolyticus 19,26%, Pseudomonas aeruginosa 10,55% and Staphylococcus aureus 4,58% and five kinds of fungi: Rhizopus sp. (37,78%), Aspergillus niger (20%), Trichosporon sp. (17,78%), Penicilliumsp. (13,33%) and Aspergillus flavus (11,11%).
PENGEMBANGAN SURVELIANS VEKTOR DALAM PENULARAN DEMAM BERDARAH DENGUE Boesri, Hasan
Jurnal Kedokteran YARSI Vol 24, No 3 (2016): SEPTEMBER - DESEMBER 2016
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v24i3.124

Abstract

Permasalahan wabah penyakit demam berdarah sering terjadi di masyarakat yang berujung dengan kematian, masih merupakan permasalahan kesehatan utama di Indonesia. Survalance entomologi yang digunakan untuk memprediksi akan terjadinya penularan DBD masih kurang peka, maka perlu adanya pengembangan sehingga diperoleh  model survalance yang lebih peka. Oleh karena itu, telah dilakukan penelitian pengembangan survalance dengan pengamatan terhadap  telur, larva, pupa, nyamuk Aedes aegypti, parity rate, dilatasi, Container indek, House indek, Breteau indek, di daerah endemis dan bebas DBD. Ternyata dari 14 indikator yang diteliti, hanya 5 indikator yang ada korelasinya dengan  kasus DBD, sedangkan yang mempunyai korelasi tertinggi (0,66 ) adalah dilatasi dua dengan kontribusi ( R ) = 66% di daerah endemis  DBD.
ANTIOXIDANT ACTIVITY AND ANTICARCINOGENIC PROPERTIES OF “SISIK NAGA”(DRYMOGLOSSUM PILOSELLOIDES PRESL.) Endrini, Susi
Jurnal Kedokteran YARSI Vol 17, No 2 (2009): MEI - AGUSTUS 2009
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.777 KB) | DOI: 10.33476/jky.v17i2.201

Abstract

The research was conducted to determine the anticarcinogenic properties of ?sisik naga?(Drymoglossum piloselloides Presl.), by the microculture tetrazolium salt (MTT) assay on the human breast carcinoma dependenthormone (MCF-7) cell lines. The preliminary results showed that the ?sisik naga? extract displayed the cytotoxic effects against MCF-7 with IC50 value of 83.63µg/ml. The antioxidative activity of the extracts which could contribute to their cytotoxic properties was also studied. The ?sisik naga? extract was found to have high antioxidant activity with IC50-value of 4.229 ppm. The strong cytotoxic properties of the ?sisik naga? extract could be due to its high antioxidant activity.
UJI MIKROPLAT AKTIVITAS ENZIM ESTERASE UNTUK MENDETEKSI RESISTENSI ANOPHELES ACONITUS TERHADAP INSEKTISIDA ORGANOFOSFAT Widiarti, Widiarti
Jurnal Kedokteran YARSI Vol 13, No 1 (2005): JANUARI - APRIL 2005
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.089 KB) | DOI: 10.33476/jky.v13i1.1045

Abstract

Non specific esterase are known to be important detoxification enzyme contributing to development of insecticides resistance in mosquitoes. Elevated esterase activity is one of the mechanisms of resistance to organophosphate, carbamate and pyrethroid insecticides in the mosquitoes. Such metabolic resistance can be detected by a microplate assay method. Mosquitoes vector resistance can occurs as a result of continuous exposure to the insecticide. Organophosphate insecticide has been used in the vector control program on Anopheles. aconitus, the malaria vector in Jepara Regency since 1983. The use of organophosphate and carbamate insecticides for five years for controlling Anopheles nigerrimus in Srilanka contribute to the selective resistance. This could happen to the population of An. aconitus in Jepara Regency. The objectives of this studies was to determine the potency of An. aconitus from Jepara Regency to be resistant to organophosphate insecticide related to esterase activity mechanism. The study methods used was biochemical assays (microplate assays )for elevated esterase. The level of esterases in larvae was determined using a and b naphthyl acetate, as the substrate andFast Blue B as the coupling reagent. The esterase activity was measured at 450 nm with Dytech ELISA plate reader. Microplate assay (Biochemical assays) on individual An. aconitus from Mlonggo II and Bangsri III subdistrics revealed that 12,9% and 28,6% population was resistant to organophosphate insecticide. The result showed that there was significant difference of elevated of esterase activity with both a and b naphthylacetate substrate hydrolysis, which appeared to be the major resistance mechanism in this multiple organophosphate resistant strain. Therefore the use of another insecticide group for vector control (An. aconitus) was suggested.
PENGARUH INFUSA WORTEL (DAUCUS CAROTA L.) TERHADAP HISTOPATOLOGI GINJAL TIKUS JANTAN YANG DIINDUKSI URANIUM W., Windhartono; Kamal, Zainul; Sasmito, Ediati
Jurnal Kedokteran YARSI Vol 21, No 1 (2013): JANUARI - APRIL 2013
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v21i1.20

Abstract

Ginjal merupakan organ vital yang berperan sangat penting dalam mempertahankan kestabilan lingkungan dalam tubuh dengan fungsi utama yaitu filtrasi oleh glomerulus, reabsorbsi dan sekresi tubulus. Kerusakan sel ginjal sampai kematian sel akan menyebabkan fungsi ginjal terganggu. Efek paparan senyawa radioaktif salah satunya dapat menyebabkan terjadinya gangguan sel-sel ginjal. Penelitian ini bertujuan untuk mengetahui pengaruh infusa wortel (Daucus carota L.) dalam mencegah gangguan sel ginjal akibat paparan uranium. Uji proteksi dilakukan dengan membagi 30 tikus jantan dewasa menjadi enam kelompok masing-masing kelompok terdiri dari 5 ekor. Kelompok I tanpa diberi perlakuan, kelompok II diberi uranium 8 ppm dosis 0,01 mL/g BB sebagai kontrol negatif, kelompok III sebagai kontrol positif dengan vitamin C 200 mg/70 kg BB yang diberikan 15 menit sebelum pemajanan uranium, kelompok IV,V, dan VI sebagai kelompok uji proteksi kerusakan sel ginjal diberi infusa wortel (Daucus carota L.) berturut-turut 10%, 20%, 30% dengan dosis 0,01 mL/g BB 15 menit sebelum diberi uranium 8 ppm dosis 0,01 mL/g BB. Lima hari kemudian hewan dikorbankan dan diambil organ ginjalnya untuk dilakukan pemeriksaan makroskopis dan mikroskopis berupa pengamatan kondisi fisik organ ginjal, pengamatan terhadap nekrosis sel ginjal, dan skoring tipe kerusakan untuk menganalisis efek proteksinya. Hasil pemeriksaan histopatologi menyimpulkan bahwa infusa wortel dapat mengurangi kerusakan sel ginjal akibat paparan senyawa radioaktif uranium.Kidney is the vital organ which has an importance role in maintaining environmental stability of the body, with the main functions are glomerulus filtration, reabsorption and secretion by tubulus. The damage of kidney cells will reduce kidney function. If a kidney is exposed to radioactive substance it could deteriorate the kidney. The aim of this research was to find out the effect of carrot infusion used to prevent kidney cells disorder due to uranium exposure. Thirty rats wistar strain divided into 5 groups were used in this study, comprising of 5 rats in each groups throughout the experiment. Group I was a normal control group. Group II was given uranium of 8 ppm 0,01 mL/g BW as negative control group. Group III was given vitamin C of 200 mg/70 kgBW 15 minute prior to uranium exposure. Group IV-VI were given carrot infusion at 10%, 20%, 30% dose of 0,01 mL/g BW 15 minutes prior to uranium exposure. The rats were sacrificed in day-5, to observe the kidney?s damage upon macroscopy and microscopy examination, involving physical condition of the kidney, necrosis of kidney cells and scoring the damage of kidney cells. The microscopic examination toward necrosis of kidney cells showed that carrot infusion can reduce cell damage due to radioactive compound of uranium.
SIMPLIFIED CLINICAL ELECTROCARDIOGRAM SCORE SEBAGAI FAKTOR PREDIKTOR MORTALITAS PADA PASIEN INFARK MIOKARD AKUT DI INTENSIVE CARDIAC CARE UNIT (ICCU) RSUD DR.SOEDARSO Nurul Yanti, Syarifah
Jurnal Kedokteran YARSI Vol 24, No 3 (2016): SEPTEMBER - DESEMBER 2016
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v24i3.312

Abstract

WHO pada tahun 2008 menyatakan tiga juta orang meninggal akibat penyakit infark miokard di seluruh dunia. Pada tahun 2013 berdasarkan Riset Kesehatan Dasar didapatkan bahwa penyakit infark miokard akut (IMA) merupakan penyakit terbanyak terdiagnosis dan menjadi penyebab mortalitas tertinggi di ICCU RSUD dr. Soedarso. Salah satu penyebab mortalitas adalah kurangnya kepekaan klinisi dalam memprediksi dan mencegah komplikasi yang terjadi pada pasien IMA. Penelitian ini dilaksanakan untuk mengetahui hubungan  Simplified Clinical Electrocardiogram Score sebagai prediktor mortalitas pasien IMA. Penelitian ini merupakan studi analitik dengan desain kasus kontrol. Penelitian dilakukan di ICCU RSUD dr. Soedarso. Sampel pada penelitian ini berjumlah 96 sampel, dibagi menjadi dua kelompok yakni 51 sampel kelompok kontrol (skor ? 4) dan 45 sampel kelompok kasus (Skor ? 5). Penelitian dilakukan dengan menghitung kelainan rekaman elektrokardiogram pasien infark miokard akut di ICCU RSUD dr. Soedarso menggunakan kriteria penilaian Simplified Clinical Electrocardiogram Score. Mortalitas yang terjadi pada kelompok kasus 35 orang sedangkan pada kelompok kontrol 6 orang. Uji statistik Chi-square menunjukkan Simplified Clinical Electrocardiogram Score memiliki hubungan yang bermakna dengan mortalitas pada pasien infark miokard akut yang dirawat di ICCU RSUD dr. Soedarso (p = 0,00), dengan OR 26,25 IK 95% 25,15-27,35. Terdapat hubungan yang bermakna antara Simplified Clinical Electrocardiogram Score dengan mortalitas pada pasien infark miokard akut yang dirawat di ICCU RSUD dr. Soedarso.
MODEL PEMBENTUKAN ADVANCED GLYCATION END PRODUCTS (AGES) DAN DEGRADASI TIROSIN AKIBAT REAKSI MAILLARD Suhartono, Eko; Setiawan, Bambang; Mashuri, Mashuri
Jurnal Kedokteran YARSI Vol 13, No 1 (2005): JANUARI - APRIL 2005
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v13i1.1050

Abstract

Maillard reaction or glycosylation reaction is a reaction between amine group and aldehyde group from glucose to form dicarbonyl compound and Advanced Glycation End Products (AGEs). The formation of AGEs have a role in diabetic complications. This work was conducted study the tyrosine degradation reaction model and the formation of AGEs caused by Maillard reaction. The research design was an experimental study with pre and posttest control group design with followed up posttest in 48 hours to 20 days. Two solutions, A and B were used; solution A was a mixture of BSA 30% + buffer phosphate pH 7 + aquadest, and solution B was BSA 30%+ buffer phosphate pH 7+ aquadest+ glucose. The degradation of tyrosine was measured by its absorbance at l=470 nm and the absorbance of AGEs formation at l=340 nm. Model of AGEs formation in solution A followed the equation of Y=0,0289X ? 0,01847 with correlation coefficient R2=0,9767, where as that in solution B followed equation of Y=0,6552X ? 1,5267 with correlation coefficient R2=0,9463. The tyrosine degradation model in solution A followed equation of Y=-0,1232X + 2,7166 with correlations coefficient R2=0,9175 where as that in solution B followed equation of Y=-0,03552X + 0,7762 with coefficient of correlation R2=0,9175. Negative value was observed in correlation between AGEs absorbance with tyrosine in solution A and B reflecting the formation of AGEs followed by tyrosine degradation
PERAN METFORMIN TERHADAP DISLIPIDEMIA ATEROGENIK PADA SINDROMA METABOLIK DENGAN DIABETES MELLITUS TIPE-2 YANG TERAWAT JELEK Sugiarto, Sugiarto; Diding HP, Diding HP; Guntur H, Guntur H; Marsetio D, Marsetio D; Tjokroprawiro A, Tjokroprawiro A
Jurnal Kedokteran YARSI Vol 17, No 3 (2009): SEPTEMBER - DESEMBER 2009
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v17i3.212

Abstract

Sindroma Metabolik dengan Diabetes Mellitus tipe-2 [SIMET-DM] merupakan kelompok faktor risiko kardiometabolik dan mempunyai peranan terhadap peningkatan penyakit kardiovaskular. Metformin merupakan obat yang mempunyai efek pleiotropik yang meliputi efek metabolik, kardiovaskular dan anti-kanker. Penelitian ini dimaksudkan untuk mengujiapakah metformin mempunyai  efek kardioprotektif melalui perbaikan dislipidemia aterogenik pada SIMET-DM tipe-2 yang terawat jelek. Penelitian dilakukan dengan metode acak, uji klinik buta-ganda di klinik Penyakit Dalam RSUD Dr Moewardi Surakarta antara bulan Desember 2008 sampai dengan Juli 2009 pada penderita SIMET-DM tipe-2 yang terawat jelek. Empat puluh dua pasien dibagi menjadi dua kelompok yaitu kelompok meformin dan plasebo, masing-masing 21 pasien. Data dianalisis dengan independent samples t-test, dilanjutkan effect size metformin dengan tingkat kemaknaan p 0,05. Hasilnya memperlihatkan bahwa secara bermakna metformin menurunkan LDL-C (p =0,012) dibanding placebo, namun tidak untuk HDL-C (p=0,151), TG (p=0,930), maupun small-denseLDL (p= 0,323). Sebagai simpulan metformin memperlihatkan efek kardioprotektif melalui perbaikan dislipidemia aterogenik (penurunan LDLC) pada SIMET-DM tipe-2 yang terawat jelek

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