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Published by Universitas Negeri Padang

ISSN : 2614669X EISSN : 2579308X DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.

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Articles 13 Documents

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Search results for , issue "Vol 3, No 2 (2019): Biology" : 13 Documents clear

Ethnobiology exploration of Suku Dayak Losarang, Indramayu District-West Java Province Lesy Luzyawati; Lissa Lissa
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Biodiversity is one of local wisdom that should be kept and maintained. Several local governments have different ways of managing and maintaining their existence, including in Krimun village-Losarang, Indramayu District, West Java Province. This study is aimed at finding the information about Suku Dayak people in Losarang (which is not same as Dayak in Borneo Island), how they manage and utilize biodiversity in their surroundings, and how it relates to the local wisdom. This is a qualitative research with instruments consists of interview sheets, field notes, and audio-visual documentation. The subject of this research is the Suku Losarang Dayak people whom are selected through incidental sampling techniques. The study of Ethnobotany includes utilization of 56 species plants as food, three species as boards, nine species for medicine, two species for aesthetics, and nine species for rituals. Ethnoecology here means the conservation of community of the Suku Dayak Losarang people toward surrounding ecosystems that are conserved for the needs of farming, shelter, and ritual. Concerning for ethnozoology, none of the species was used because they have a principle "ngaji rasa". This principle means that they would not be allowed to consume or to make use of the living creatures.

Ethnoveterinary Pharmacology: Knowledge Identification of Sukarame Society, Carita, Pandeglang, Banten Rizhal Hendi Ristanto; Lia Junita Harahap; Elsa Lisanti
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The use of chemical medicine in livestock soared. The price of expensive chemical medicine does not discourage people from turning to herbal medicines. The purpose of this study is to determine the level of public knowledge of ethnoveterinary pharmacology and diversity of herbal medicines in the village of Sukarame. This research is quantitative descriptive. Data was collected through observation, semi-structured interviews and identification. The sample in this study was the people in Sukarame village and the types of herbal medicines in Sukarame village. Data from the community was obtained through interviews and observations with the Purposive Sampling technique and data on the type of ethnoverinary obtained from identification. The results showed that public knowledge about ethnoveterinary pharmacology obtained an average score of 55%. The informants who provided data were men at 71% of the total informants. The types of herbal medicines used by the public are Zingiber officinale, Curcuma domestica, Physalis angulata, Piper betle.

Antifungal Activity Test of Cinnamon Extract (Cinnamomun burmani) on Growth of Aspergillus flavus and Fusarium moniliforme Vivi Novrianti; Israwati Harahap; Elsie Elsie
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The purpose of this research is to determine the activity of cinnamon extract (Cinnamomun burmani) as an antifungal against the growth of fungi Aspergillus flavus and Fusarium moniliforme. The procedure begins with making cinnamon extract obtained from cinnamon bark using rotary distillation method. Cinnamon extract will be tested using different concentrations of 0% (control); 0,5%; 1,0%; 2,0% and 4,0% with the well diffusion method. Based of the results, the cinnamon extract has antifungal activity against the fungi with the concentration and inhibition of 1,0% and inhibition zone diameter of 27 mm in A. flavus, while the concentration and inhibition of 2,0% with a inhibition zone of 56 mm in F. moniliforme.

Chlorophyll Content of Jabon Leaves (Anthocephalus cadamba [Roxb] Miq.) in the Sungai Nyalo, Pesisir Selatan and Lubuk Alung, Padang Pariaman Vauzia Vauzia; Resti Fevria; Yovella Trisna Wijaya
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Jabon (Anthocephalus cadamba [Roxb] Miq.) is one type of tree that has a high prospect for industrial plantations and reforestation in Indonesia, due to its very fast growth and relatively easy silvicultural treatment. The growth of a plant is strongly influenced by photosynthesis. The process of photosynthesis can take place because of the presence of pigments called chlorophyll. The formation of chlorophyll is influenced by environmental factors. Therefore, studies have been carried out on the chlorophyll content of Jabon leaves in different locations. This research was conducted from March to May 2019. The samples came from the Sungai Nyalo, Pesisir Selatan, and Lubuk Alung, Padang Pariaman. While the chlorophyll content of Jabon leaves was tested at the Laboratory of Plant Physiology, Department of Biology, FMIPA UNP. This research is a descriptive study that was analyzed by the T test at a significant level of 5%. The results showed that there were differences in the chlorophyll content of Jabon leaves in the Sungai Nyalo, Pesisir Selatan with value 44,26 (mg/g) and Lubuk Alung, Padang Pariaman with value 19,45 (mg/g).

Prediction Potential Chlorogenic Acid As Inhibitor Ace (In Silico Study) Yohanes Bare; Dewi Ratih Tirto Sari; Yoga Tribakti Rachmad; Sri Sulistyaningsih Natalia Daeng Tiring; Apriani Herni Rophi; Fitra Arya Dwi Nugraha
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

One of the derivatives of flavonoid explored is chlorogenic acid. Chlorogenic acid has antioxidant and anti-inflammatory activity. Hypertension has support for Angiotensin converting enzyme (ACE) which has a role in regulating the renin-angiotensin system. Hypertension therapy is carried out in inhibit ACE pathway. This study aims to analyze and assess the potential of chlorogenic acid as an anti-hypertensive material by inhibiting the work of ACE. The method used is in silico. Chlorogenic acid ligand was obtained from PubChem while ACE was obtained from RCSB. Interaction of ligand and protein using HEX 8.0.0. Analysis and visualization of the results of interactions using Discovery Study Version 4.1. The results showed an interaction between ligand and protein, namely interactions that occur between chlorogenic acid and sixteen amino acid residues. This interaction produces energy of -292.5cal / mol. This interaction approved the ACE block in the AT-I transformation towards AT-II. Chlorogenic acid has potential as an anti-hypertension material.

Growth Response Test on Potential Indigenous Bacteria to Degrade Naphthalene and Phenanthrene on various pH Alfi Rizca Hardianti; Helga Lusiana; Dita Widayanti Sawitri
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

This study aims to understand the growth response of indigenous bacteria originating from oil sludge in Dumai which has the potential as Naphthalene and Fenantren degrading agents based on variations in substrate pH. The isolates used in this study were Isolate A, Isolate E, and Isolate F as a result of isolation from oil sludge in Dumai. Based on the results of the growth response test on various concentration in previous studies, we chose E isolates to be tested for its degradation ability. The bacterial growth response data was obtained based on OD measurements every 24 hours using a spectrophotometer and TPC at 72 hours using Nutrient Agar media. Based on these data, we determined the optimum pH for indigenous bacterial growth potential to degrade naphthalene or phenanthrene. In all variations of pH, bacterial isolate E experienced growth. This shows that bacterium E can use naphthalene and fenantren at normal pH range (pH 5-9).

Screening of Probiotic Bacteria Candidates in The Mangrove Tourism Area in Klawalu Sorong City West Papua Sukmawati Sukmawati; M Iksan Badaruddin
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Probiotic bacteria was one of the biological control agents (biological control). It had a role in suppressing or killing pathogenic microorganisms. In addition, it also played a role in improving water quality as in fish maintenance media. The basic principle of probiotics was the utilization of the ability of microorganisms to increase absorption in the digestive tract of fish. Probiotic bacteria were able to produce antimicrobial compounds such as lactic acid, hydrogen peroxide, bacteriocin. These compounds were antimicrobial and antibiotic that can suppress the growth of pathogenic bacteria. The purpose of this study was to detect candidate probiotic bacteria from the mangrove tourism area in Klawalu, Sorong City, West Papua. The results of screening for probiotic bacteria candidates by using selective media, from 16 samples consisting of water samples and mud samples’ obtained 11 positive samples, there were probiotic bacteria candidates with the bacterial morphological characteristics of each colony was almost similar. Keywords: Probiotic Bacteria, Mangrove Land, Sorong City

Diversity Of Nocturnal Insects (Insecta) In Bukik Kasang, Padang Pariaman, West Sumatra Emelia Debora; Rijal Satria
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The study of nocturnal insect diversity was conducted in Bukik Kasang, Padang Pariaman District, West Sumatra from April to May 2019. The pitfall trap was used to collect the active insect on the ground in three different habitats: open area, edge of plantation, and inside the plantation. A total 10 orders, 44 morphospecies, and 180 individual of nocturnal insect was collected. The Order Hymenoptera was the highest in number of morphospecies and individual, and in other hand, the Order Hemiptera was the lowest one. This study is very important to reveal the diversity of insect in Bukik Kasang, due to the increasing of human activities in this area and planning to develope this area as tourist destination.

Isolation and Characterization of Lactic Acid Bacteria (Lactobacillus sp) from Sauerkraut with the addition of Cayenne Pepper Resti Fevria; Indra Hartanto
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0201932106355-0-00

Abstract. Sauerkraut is cabbage that is produced from natural fermentation by bacteria in the presence of 2.5 percent salt. Salt additions limits the activity of gram –negative bacteria, while the growth of lactic acid bacteria will increase. Chili is a vegetable of the genus Capsicum which has high economic value and also contains various compounds that are useful for human health. One method to get good quality cayenne is fermentation. Fermentation is part of biotechnology that uses microorganisms as the main actors in a process. One of the microbes that determines the success of fermentation is lactic acid bacteria. The purpose of this study is isolate LAB from Sauerkraut with the additional cayenne pepper the type of LAB produced microscopically. Research methods, the ingredients used are cabbage and cayenne fermentation (sauerkraut with the additional cayenne pepper), MRSa, 0,9% NaCl, crystal violet paint from biological laboratories UNP. Isolation LAB from Sauerkraut done in with fermentation Sauerkraut and then plant the sauerkraut into the MRSa medium with streak plate methods. The isolates obtained were identified microscopically using a microscope with gram staining method. From the research that has been done, the following result are obtained : Sauerkraut with additional cayenne pepper direcly into MRSa medium and gram staining, there were 12 colonies of gram positive bacteria with bacil cell form, and negative catalase test. We can identify this colonies as Lactobacillius sp.

Production of Complex Amylase Enzymes From Aspergillus Awamori KT-11. Its Application To Hydrolyze Cassava and Taro Starch Ruth Melliawati; Nuryati Nuryati
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Indonesia as an agricultural country rich in various types of plants including tubers. The purpose of this study was to produce and apply complex amylase enzymes from Aspergillus awamori KT-11 in hydrolyzing cassava and taro starch to liquid sugar. The production of complex amylase enzymes is carried out in a liquid and solid medium. The hydrolysis of starch into sugar is carried out with several enzyme concentrations (2-10%) and starch concentrations (5-25%) at room temperature (30ºC) and 60⁰C for 3 hours, 24 hours - 72 hours. TLC tests were carried out on sugar from hydrolysis of cassava starch and taro flour to determine the type of sugar formed.As a result, the highest enzyme activity was obtained in the solid medium formation (242.83 Units/ml). The best condition for hydrolyzing starch is 15% starch concentration, 10% enzyme concentration at 60ºC for 72 hours. The results of hydrolysis sugar were 9.52 mg / ml (taro) and 9.37 mg/ml (cassava) respectively, with an incubation time of 72 hours.The TLC analysis showed that sugar Rf from cassava was detected as lactose and Rf from Talas flour as maltose.

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