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Bioscience
Published by Universitas Negeri Padang
ISSN : 2614669X     EISSN : 2579308X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.
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Articles 146 Documents
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Potensi Bakteri Metanotrof sebagai Pereduksi Emisi Metan pada Lahan Pertanian Febrianti Rosalina; Sukmawati Sukmawati; Ponisri Ponisri; Anif Farida; Budi Satria; Ayu Diah Syafaati; Nuryanto Nuryanto
Bioscience Vol 7, No 1 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371120929-0-00

Abstract

Konsentrasi Gas Rumah Kaca (GRK) meningkat seiring dengan aktivitas manusia dan menyebabkan peningkatan pemanasan global, salah satunya berasal dar sektor pertanian. Masih tingginya produksi emisi GRK pada sektor pertanian membutuhkan monitoring dan pengawasan secara berkala, sehingga dapat dipantau dan ditekan kuantitasnya. Untuk mengukur emisi GRK diperlukan inovasi dengan salah satu pengaplikasian bakteri metanotrof yang dapat menekan emisi di lahan pertanian. Tujuan dari penelitian ini adalah untuk mengetahui potensi bakteri metanotrof yang diaplikasikan pada lahan pertanian dalam mengurangi emisi metan. Perlakuan dalam penelitian terdiri dari 4 perlakuan diantaranya Sungkup 1 (Isolat bakteri MFb), Sungkup 2 (Isolat bakteri MFc), Sungkup 3 (Isolat bakteri MFd), dan Sungkup 4 (Isolat bakteri MFe). Pengambilan contoh gas dilakukan dengan metode sungkup tertutup (close chamber technique). Emisi metan (CH4) dianalisis secara langsung di lapangan dengan menggunakan alat digital berupa Alat Pintar Digital deteksi Kebocoran Gas Metana dan Propana AZ-7291 untuk mengukur CH4. Hasil penelitian menunjukkan bahwa terdapat pengaruh aplikasi bakteri metanotrof terhadap laju penurunan emisi gas metan. Dari semua perlakuan isolate bakteri metanotrof  yang diberikan, perlakuan bakteri metanotrof dengan kode MFe mampu menurunkan rata-rata emisi CH4 sebesar 305,449 mol/jam dan dianggap bahwa isolate tersebut adalah isolate yang paling baik diantara semua perlakuan.Greenhouse Gas (GHG) concentrations increase along with human activities and cause an increase in global warming, one of which comes from the agricultural sector. The high production of GHG emissions in the agricultural sector requires regular monitoring and supervision, so that the quantity can be monitored and suppressed. To measure GHG emissions, innovation is needed, one of which is the application of methanotrophic bacteria which can reduce emissions on agricultural land. The purpose of this study was to determine the potential of methanotrophic bacteria applied to agricultural land in reducing methane emissions. The treatment in this study consisted of 4 treatments including Chamber 1 (bacterial MFb isolate), Chamber 2 (bacterial MFc isolate), Chamber 3 (bacterial MFd isolate), and Chamber 4 (bacterial MFe isolate). Gas sampling was carried out using the closed chamber technique. Methane (CH4) emissions are analyzed directly in the field using a digital device in the form of a Methane and Propane AZ-7291 Digital Smart Leak Detection Tool to measure CH4. The results showed that there was an effect of the application of methanotrophic bacteria on the rate of reduction of methane gas emissions. Of all the isolates of methanotrophic bacteria given, the treatment of methanotrophic bacteria with the code MFe was able to reduce the average CH4 emission by 305.449 mol/hour and it was considered that the isolate was the best isolate among all the treatments.Keywords: Methanotrophic bacteria, GHG, methane, agricultural land
Polymerase chain reaction (PCR) primer design to identify SNP rs7901695 transcription factor 7 like 2 (TCF7L2) Elsa Badriyya; Afifatul Achyar
Bioscience Vol 7, No 1 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371122575-0-00

Abstract

ABSTRACT. Transcription Factor 7 Like 2 (TCF7L2) is a gene that produces a protein that controls the expression and function of several incretin hormones. One of the primary activities of incretin hormones is to increase insulin production, which is important in glucose and energy homeostasis. Single Nucleotide Polymorphism (SNP) in the TCF7L2 gene is reported to be association with Type II Diabetes Mellitus (T2DM). T2DM is featured by persistent hyperglycemia because of the decrease in insulin production, insulin resistance, or both. In patients with diabetes mellitus, chronic hyperglycemia can damage organ systems and cause metabolic abnormalities. SNP rs7901695 TCF7L2 gene has been linked to T2DM in a number of places, including South Asia, Iceland, and the United States. The polymorphism at location 112.994.329 from nucleotide Timine (T) to Cytosine (C) enables the recognition of SNP rs7901695. Polymerase Chain Reaction (PCR) was used to identify the polymorphism. The objective of this research was to generate a precise primer for the PCR recognition of the SNP rs7901695 in the TCF7L2 gene. The research's methodology comprises DNA isolation, primer designing with Geneious, target amplification with PCR, and DNA sequencing for bioinformatic analysis. As a result of the study, four primers for the SNP rs7901695 TCF7L2 gene have been developed. The reaction obtained two fragments, sized 177 and 367 bp. The primers used were rs7901695-F, rs7901695-R, rs7901695-F(C), and rs7901695-R(T), which were used to detect the T allele.  ABSTRAK. Transcription Factor 7 Like 2 (TCF7L2) adalah gen yang menghasilkan protein yang mengontrol ekspresi dan fungsi beberapa hormon inkretin. Salah satu aktivitas utama hormon inkretin adalah meningkatkan produksi insulin, yang penting dalam homeostasis glukosa dan energi. Single Nucleotide Polymorphism (SNP) pada TCF7L2 gene dilaporkan adanya hubungan dengan Diabetes Mellitus Tipe II (DMT2). DMT2 ditandai dengan hiperglikemia persisten karena penurunan produksi insulin, resistensi insulin, atau keduanya. Pada penderita diabetes melitus, hiperglikemia kronis dapat merusak sistem organ dan kelainan metabolisme. Gen SNP rs7901695 TCF7L2 dilaporkan memiliki asosiasi dengan DMT2 di sejumlah daerah, termasuk Asia Selatan, Islandia, dan Amerika Serikat. SNP rs7901695 ditandai dengan polimorfisme pada lokasi 112.994.329 dari nukleotida Timin (T) menjadi Sitosin (C). Metode yang digunakan untuk mengidentifikasi polimorfisme adalah Polymerase Chain Reaction (PCR). Penelitian ini bertujuan untuk menghasilkan primer yang tepat untuk pengenalan PCR terhadap SNP rs7901695 pada gen TCF7L2. Metodologi penelitian meliputi isolasi DNA, perancangan primer dengan aplikasi Geneious, amplifikasi target dengan PCR, dan sekuensing DNA untuk analisis bioinformatik. Sebagai hasil penelitian, empat primer untuk deteksi gen SNP rs7901695 TCF7L2 telah dikembangkan. Dua fragmen, berukuran 177 dan 367 bp, diperoleh dari reaksi tersebut. Primer yang digunakan adalah rs7901695-F, rs7901695-R, rs7901695-F(C), dan rs7901695-R(T), yang digunakan untuk mendeteksi alel T.
PENGARUH PEMBERIAN PUPUK ORGANIK CAIR KULIT PISANG TANDUK (Musa paradisiaca) DAN DAUN Moringa oleifera TERHADAP PERTUMBUHAN TANAMAN KANGKUNG DARAT (Ipomoea reptans POIR.) DENGAN MENGGUNAKAN MEDIA KULTUR AIR Hayati, Annida Putri; Maharani, Dhea Tinde Fitriana; Rahmawati, Fitriana; Fauziah, Karyna; Helmi, Haidar; Zanah, Nurul Fatihal; Khairunnisa, Zahra Syafira; Rahman, Taufik; Suwandi, Tri
Bioscience Vol 8, No 2 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371121503-0-00

Abstract

Kangkung darat (Ipomoea reptans Poir) adalah salah satu jenis kangkung yang mudah dibudidayakan meskipun begitu, di kota besar sulit menanam kangkung karena keterbatasan lahan. Digunakanlah metode kultur air dalam budidayanya. Penelitian ini bertujuan untuk mengetahui pengaruh pupuk organik cair (POC) berbahan dasar kulit pisang (Musa paradisiaca) dan daun kelor (Moringa oleifera) terhadap pertumbuhan kangkung darat dengan menggunakan metode kultur air.  Penelitian dilakukan selama 14 hari, di laboratorium Fisiologi FPMIPA Universitas Pendidikan Indonesia. Penelitian ini menggunakan metode Rancang Acak Lengkap (RAL) dengan 3 kali pengulangan dari setiap perlakuan. Parameter penelitian ini yaitu jumlah daun, tinggi cabang, panjang akar, jumlah cabang, berat basah batang dan berat basah akar. Data dianalisis dengan menggunakan uji Anova pada taraf 95%. Dari hasil penelitian diketahui bahwa pupuk POC tidak memiliki pengaruh nyata terhadap jumlah daun, tinggi cabang, panjang akar, jumlah cabang, berat basah batang dan berat basah akar. Tetapi pengaruh dapat dilihat apabila dibandingkan dengan kontrol.Kata kunci: Kangkung darat, Kultur air, Musa paradisiaca,  Moringa oleifera
Structure of Tree Community on Agroforestry Parak in Peri-Urban Areas District of Lubuk Alung Padang Pariaman Reki Kardiman; Irma Leilani
Bioscience Vol 7, No 1 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371122621-0-00

Abstract

Agroforestry or tree crops in West Sumatra is called parak, it mostly related to rural and farmers but at some developing areas the parak is still existed with a high potential changes in structure and floristic composition due to commercial orientation. To explore the possible changes of tree community in such developing areas, 11 of 20x20 meter plots were established across parak areas in peri-urban site in Lubuk Alung district, Padang Pariaman Regency, West Sumatra Indonesia, aimed to specifically investigate tree density, stand basal area, spesies distribution and other ecological atributes of the species. We found 24 tree species from 306 trees within 0.44 ha or about 695 tree per ha. Durio zibethinus was the biggest trees, found as a top canopy but few in number and spreaded quite widely across the areas, followed by some other species such as Cocos spp., Areca catecu, Garcinia spp., while small trees, high density but only spreaded at some places was represented by Theobroma cacao. Many species and the structure were typically agroforest that vary in canopy level and economic functions except some species which may be additionally planted to suit the needs of peri-urban community.
Medicinal Plants Used for Traditional Medicine in Ethnobotanical Studies in Nagari Padang Laweh, Sijunjung Regency Gina Julia; Nursyahra Nursyahra; Zikra Zikra
Bioscience Vol 7, No 1 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371122501-0-00

Abstract

This studi aims to determine medicinal plants used for tradisional medicine in ethnobotanical studies in Nagari Padang Laweh, Sijunjung Regency. This information can be obtained by the community as a reference for traditional medicine that has been passed down from generation to generation. The method used in this research is descriptive survey method. Medicinal plant species were identified in the field and continued at the Botanical Laboratory of PGRI University, West Sumatra. In this study obtained 81 species of medicinal plants. Not all medicinal plants used in Nagari Padang Laweh are also used as medicine elsewhere, so this knowledge can be used as an asset and needs to be preserved.
Flowering Behavior, Fruit Harvesting Age and Proximate Levels of the Flesh of the West Sumatera Superior Avocado farihul ihsan; Sri Hardiati; Ni Luh Putu Indriyani
Bioscience Vol 7, No 1 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371122130-0-00

Abstract

The aim of this study was to determine flowering behavior, fruit harvesting age and proximate levels of the flesh of the four West Sumatera superior avocado varieties, i.e: Mega Gagauan, Mega Murapi, Mega Paninggahan and Tongar. The research was conducted in Indonesian Tropical Fruit Research Institute from October 2020 to August 2022. The plant materials was used Mega Gagagauan, Mega Murapi, Mega Paninggahan and Tongar which 2 trees each variety. Parameters observed i.e: flower type, blooming time, fruit age, fruit character, and proximate content of flesh. Data were analyzed descriptively. The results showed that Mega Gagauan, Mega Murapi and Mega Paninggahan had type B flowers, while Tongar had type A flowers. The time difference between the first and second blooms on the same day for Mega Gagauan and Mega Murapi was 1 and 2 hours, while there is no time difference in Mega Paninggahan and Tongar. The fruits harvesting age of Mega Gagauan, Mega Murapi, Mega Paninggahan, and Tongar are i.e: 161; 232; 160; and 170.42 days. Tongar had the lowest water content (73.83%) and the highest fat (15.30%), Mega Murapi had the lowest crude fiber content (2.82%), and Mega Paninggahan had the highest protein content (1.87%).  Mega Paninggahan was considered the best variety based on the observed character and this research data can be used as the basic for determining actions of cultivating avocado plant.
Antimicrobial Potential Of Endophyte Bacteria In Angsana Plants (Pterocarpus indicus Willd) Nurhasnah Nurhasnah; Media Roza; Milya Sari; Kencanawati Kencanawati
Bioscience Vol 8, No 1 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371121349-0-00

Abstract

The discovery of antimicrobial active compounds is one of the pressing things in the world of medicine and the pharmaceutical industry, due to the increasing and widespread resistance of pathogenic bacteria to existing antimicrobials. Angsana plant (Pterocarpus Indicus Willd) has been shown to have efficacy as a drug, so it has the potential to be used as a source of natural antibiotics. This study aims to find out the types of endophytes in various parts of the Angsana plant (Pterocarpus indicus Willd) through macroscopic and microscopic identification and find out the activity of antimicrobial compound-producing endophye bacteria in Angsana plants. Bacterial purification techniques use streak plate and spread plate methods. Antimicrobial activity tests are carried out using diffusion methods by means of point inoculation. The results showed 29 isolates of endophye bacteria isolated from the Angsana plant (Pterocarpus indicus Willd). From the roots as many as 11 isolates, stems as many as 12 isolates, and leaves as many as 6 isolates. The result of Gram staining of Angsana plant endophyte bacteria, obtained 22 bacterial isolates including Gram positive and 7 is Gram negative. There were 21 bacil-shaped endophyte bacterial isolates, 7 coccus-shaped isolates and 1 coccobacil-shaped isolate. Isolate the endophyte bacteria of Angsana plants that have the potential to produce antimicrobial compounds as many as 20 isolates. Isolates of Angsana plant endophyte bacteria form a bland zone in    S. aureus (10 isolates), E. coli (17 isolates). Endophyte bacterial isolates that are able to inhibit the growth of bacteria S. aureus and  E. coli are 7 isolates
Specific primer design and optimization of annealing temperature for amplification gene peroxidase (POD) in Oryza sativa L. Nella Fauziah; Afifatul Achyar; Zulyusri Zulyusri; Yusni Atifah; Linda Advinda; Violita Violita
Bioscience Vol 7, No 2 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v7i2.122972

Abstract

Peroksidase (POD) merupakan enzim antioksidan yang memiliki beragam fungsi dalam siklus hidup tanaman, salah satunya adalah sebagai pertahanan dalam melawan ROS dengan mengkatalisis konversi H2O2 menjadi udara dan O2 . Kemampuan aktifitas enzim POD dalam mengatur kandungan H2O2 memungkinkan enzim tersebut dapat mempertahankan tanaman dari cekaman. Metode yang dapat digunakan untuk amplifikasi gen POD salah satunya yaitu kuantitatif reverse transcription- PCR (qRT-PCR). Metode ini memerlukan beberapa komponen penting salah satunya yaitu primer ( forward dan reverse ). Primer yang digunakan dalam amplifikasi gen harus spesifik terhadap gen target sehingga dapat mengenali dan menempel pada gen target yang diinginkan. Penelitian ini bertujuan untuk mendesain primer yang sesuai untuk amplifikasi gen POD menggunakan teknik qRT-PCR. Primer dirancang menggunakan perangkat PrimerQuest. Primer yang telah dirancang kemudian dianalisis untuk spesifikasinya dengan geneious prime . Kemudian spesifikasi primer di cek menggugakan primer BLAST. Hasil desain primer dengan kriteria terbaik untuk amplifikasi gen POD yaitu Forward POD 5'-AAATGCGTCGATCTACTGTACCT-3' dan Reverse POD 5'-GTGTTGAAAATGGCAATAAACCGG-3'
Rice Growth Response to Drought Simulation Treatment Using PEG Annisa Khaira; Zulyusri Zulyusri; Afifatul Achyar; Dwi Hilda Putri; Yusni Atifah; Violita Violita
Bioscience Vol 7, No 2 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v7i2.122674

Abstract

Rice is the main food source for Indonesians. The demand for rice to meet people's needs increases every year due to population growth and efforts to improve nutrition by the community. One of the problems that can affect rice production is drought. Drought is an environmental condition when plants do not get enough water to grow and develop optimally, which can cause a decrease in rice production. To find out how rice growth responds to drought, a study was carried out by giving drought simulation treatments using polyethylene glycol (PEG) on several rice varieties. This study used a completely randomized design that was arranged in a factorial manner with two factors. The first factor was the rice varieties (Harum, Situbagendit, Rosna) the second factor was 0% and 20% PEG concentration. The data obtained were then analyzed statistically using a two-way ANOVA test, and if the results were significantly different, then proceed with Duncan's test at the 5% level.The results showed that the drought simulation treatment had a negative effect on rice growth. Drought simulation treatment using 20% PEG resulted in a decrease in Kadar air relatif (KAR), root length, plant height, and root dry weight of rice. The highest decrease in KAR was found in the sensitive rice variety (Rosna), which was 43.42%. The highest average root length (7.99 cm) was on the sitabaendit variety, and the lowest (5.61) was on the rosna variety. The highest average crown height (17.32 cm) and the lowest (6.61) were on the rosna variety.
Multiple Alignment and Primer Design for Groups of Barnacle Organisms and Amphibalanus amphitrite as Biofouling Markers Muhammad Farikh; Rahmad Wanizal Pastha; Rezeki Rival Alridho; Aura Zahra Nafisah; Bintang Fadhil Ramadhan; Afifatul Achyar
Bioscience Vol 7, No 2 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v7i2.125392

Abstract

Biota pengotor atau biofouling adalah organisme hidup yang menempel pada suatu substrat. Dampak yang ditimbulkan oleh aktivitas biofouling biota adalah oleh karena sifatnya yang korosif sehingga dapat menyebabkan kelumpuhan pada bangunan instalasi. Untuk memfasilitasi instalasi dan kestabilan biodiversitas, peneliti mencoba menghadirkan environmental DNA (e-DNA) sebagai sampel lingkungan tanpa membunuh dan mengisolasi suatu organisme tersebut dengan memanfaatkan sampel lingkungan. Penelitian ini bertujuan untuk memperoleh primer universal dan spesifik dari COX1 yang dapat digunakan untuk mengidentifikasi biofouling kelompok Barnacle yang dirancang secara in silico. Sekuen organisme target yang digunakan dalam penelitian ini adalah DNA mitokondria dari Amphibalanus amphitrite, Amphibalanus reticulatus dan Semibalanus balanoides dengan nomor aksesi NC_024525, NC_071896 dan NC_039849.. Sekuen gen COX1 yang diperoleh disimpan dalam format FASTA untuk digunakan lebih lanjut di dalam proses desain primer menggunakan Geneious Prime. Penentuan daerah lestari ditentukan dengan Geneious Prime dalam tahapan multiple alignment. Sekuen dengan kriteria primer spesifik terbaik untuk COX1 A.amphitrite didapatkan dengan panjang 20-22 basa dan ukuran amplikon 910 bp. Urutan basa primer foward dan reverse adalah 5'-GAGCTGAACTTGGTCAACCG-3' dan 5'-GCTCAAAGAAGAGGAGGGCTAT-3'. Sekuen dengan kriteria primer universal kelompok barnacle terbaik untuk COX1 didapatkan dengan panjang masing-masing 24 basa dan ukuran amplikon 1.270 bp. Urutan basa primer foward dan reverse adalah 5'-GACTTCTACCGTTAATGTTAGGAG-3''dan 5'-CTATGGTAATGAGGAGGAGTAGTG-3'. Hasil desain memenuhi syarat kriteria primer yang baik sehingga kandidat primer hasil desain dapat digunakan untuk proses PCR.

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