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Published by Universitas Negeri Padang

ISSN : 2614669X EISSN : 2579308X DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.

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Articles 146 Documents

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The Effect of Mango Juice (Mangifera Indica) and Long Ripening on the Quality Of Cow's Milk Curd Selly Fildawati; Linda Advinda; Azwir Anhar
Bioscience Vol 1, No 2 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The curd is a traditional food Minangkabau in West Sumatra. This product is made from buffalo milk made with buffalo milk entering into the bamboo and covered with banana leaves and left at room temperature for about 1 to 2 days. Due to the limited availability of buffalo milk, buffalo milk is replaced with cow's milk. Obstacles encountered in the development of the curd is curd less flavorful savory and original taste sour curd so that the public interest is reduced. Therefore, we need a way to neutralize the smell and taste of sour curds, one of which is the juice of mango (Mangifera indica). The purpose of this study was to determine the effect of mango juice and long ripening on the quality of cow's milk curd. This study is experimental and descriptive. The design used was a 3x2 factorial randomized block design with three replications. A factor is the mango juice consisting of 3 treatment, and long ripening factor B is composed of 2 treatments. Research was conducted in January 2016 in the Laboratory of Livestock Product Technology Faculty of Animal UNAND. Parameters measured were total acid content, protein content, and organoleptic. Data were analyzed by descriptive qualitative analysis of variance (ANOVA) and a further test DNMRT at 5% level. The results of this study are the addition of mango juice and long curing does not affect the total acid content (% TAT). However, the addition of mango juice and long curing effect on protein levels although there is no interaction between the concentration and duration of ripening. Lowest total acid level was the control treatment on a long curing 48 hours and total acid concentration is the highest concentration of 3% at 36 hours. Lowest protein content that is the control treatment at 36 hours and the highest concentration of 3% at 48 hours. The results of organoleptic tests show the variation of each treatment. Keyword: cow's milk curd, mango juice, total acid content, protein content, organoleptic.

Antioxidant Activity, Antibacterial Activity, Water Content, and Ash Content In Baduy Honey Adri Nora; Anjas Wilapangga; Titta Novianti
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Indonesia as a tropical country has many different types of honey. Honey was known to have many advantages especially in health promotion as antioxidant and antibacterial. Baduy honey is one of Indonesian forest honey which its nutritional value, antioxidant, and antibacterial activity have not been investigated yet. The purpose of this study to determine antioxidant activity, antibacterial, water content, and also ash content of baduy honey. The research method are DPPH radical scavenging-activity for antioxidant, microplate resazurine assay for antibacterial, qualitative photochemistry analysis, and gravimetric analysis for measuring water and ash content. The result showed that black and yellow baduy honey has water content 18,64% dan 19,32% respectively and ash content 0,32% dan 0,04% respectively. Baduy honey has also containing flavonoid, terpenoid, and alkaloid compounds. Black Baduy honey has higher antioxidant activity than Yellow Baduy honey in which EC 50 values are 1000,79 μg/mL and 1475,28 μg/mL respectively. However, Baduy honey did not show any antibacterial activity againts Escheria coli dan Staphlococcus aureus because of MIC value for both type of honey are tremendously large with 500000 μg/mL.

Inventory of Ferns (Pteridophyta) at Cerme Cave Bantul District Zuchrotus Salamah; Hadi Sasongko; Aulida Zulaikha Hidayati
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Cerme Cave situated at Srunggo, Selopamioro village, Imogiri, Bantul District. Bantul has a supporting environment condition for ferns to grow and thrived. This research is conducted to produce an inventory of ferns grow within the Cerme Cave area. This research used explorative-method which include exploration, inventory, and fern identification activities. Descriptive analysis is used for data analysis. Result of this research showing that there are 17 species found within the Cerme Cave area, which are Drynaria quercifolia (Linnaeus) J. Smith, Pyrrosia longifolia (Burm. f.) C.V. Morton, Athyrium sp., Adiantum induratum Chirt, J. Bot. (Morot), Adiantum capillus-veneris Linn., Selaginella sp., Selaginella indica (Milde) R. M. Tryon, Adiantum philippense Linn., Pyrrosia petiolosa (Christ) Ching, Nephrolepis cordifolia (Linnaeus) C. Presl, Pteris vittata Linn., Pneumatopteris sp., Adiantum malesianum J. Ghatak, Hymenasplenium sp., Pneumatopteris sp., Pyrrosia nummularifolia (Sw.) Ching, Pteris sp.. Kata kunci: Cerme Cave, Pteridophyta

Morphological Indices of Drought Tolerant of Some Paddy Varieties (Oryza sativa L.) In West Sumatera Using Standard Evaluation System (SES) For Rice syauli mardita; Violita Violita
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Rice is an important food crop that consumed by more than half of the world's population, but almost every year there is decrease rice production in field, this is due availability of land and water as main source of agriculture. Therefore optimization of dry land as agricultural is needed, but many things are of concern to dry land, especially water sources are minimal. Therefore, it is need have rice seeds are suitable and adaptive to dry land, the response of rice plants to drought can observed from physiological, morphological and anatomical changes. This research was conducted in August to December 2018 in research laboratories, wire houses and plant physiology laboratories, Faculty of Mathematics and Natural Sciences, Padang State University. This study was to obtain a drought tolerant morphological index from several paddy seeds in West Sumatera, so indexes showed that rice seeds were tolerant to drought by observing morphological changes, namely leaf rolling (DPD), shoot top index (IKP). ), and plant recorvry (TP) using the SES (Standard Evaluation System) method for rice. Data is processed using cluster analysis with hierarchical analysis methods. The results showed the most resistant varieties to drought stress were’s Baroto, Situ Bagendit, Randah Kuniang, and Harum.

Effect of Hydrogen Peroxide (H2O2) on Different Levels of Water Availability on Vegetative Growth of Soybean Plants [Glycine max (L.) Merr.] Argomulyo Varieties Ika Shintya; Sri Widodo Agung Suedy; Endah Dwi Hastuti
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The soybean [Glycine max (L.) Merr.] is one of the important sources of vegetable protein and agricultural commodities in Indonesia, but its productivity is uncertain due to various factors, one of which is the availability of water. Low water availability in the growing environment can cause soybean plants to suffer from drought stress. Giving hydrogen peroxide to plants experiencing drought stress in optimum concentration can increase the plant's oxidative defense system. This study aims to determine the effect of treatment on the vegetative growth of Argomulyo variety soybean plants. The study used a factorial completely randomized design (CRD) design with 2x4 treatment and 5 replications. The parameters observed were plant height, number of leaves, leaf area, and fresh dry weight of the root canopy at the level of water availability 100% and 35% field capacity by spraying hydrogen peroxide concentrations of 0 mM, 0.5 mM, 1 mM, and 2 mM. The results were analyzed by ANOVA and DMRT test with a significance level of 0.05. The level of water availability influences plant height, number of leaves, leaf area, and fresh dry weight of the root canopy. The results show a 100% water availability level has a higher yield. Keywords: soybean, hydrogen peroxide, stress, growth

Efisiensi Penggunaan Nitrogen (Nue) Dan Resorpsi Nitrogen Pada Hutan Taman Nasional Bukit Duabelas Dan Perkebunan Kelapa Sawit Di Kabupaten Sarolangun, Provinsi Jambi Violita Violita
Bioscience Vol 1, No 1 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Nitrogen use efficiency (NUE) plays an important role on plant adaptation to the low nutrient conditions. Transformation system from natural forest to oil palm plantation changedthe NUE and soil nutrition in ecosystem. However, how the transformation system affects NUE, N resorption, and soil nutrition in Sarolangun, Jambi province still unknown. The aims of the study were (1) to quantify NUE and Nresorptionand its correlation to N soil content (2)to determine nitrogen loss of litterfall in natural forest (HA) and oil palm plantation (KS) in Sarolangun, Jambi. The result showed that NUE in plant scale (NUE c) influenced by N resorption. NUE c increased with increasing of N resorption. Nitrogen and carbon content of the soil in HA was higher than that of in KS. There was no correlation between N soil content with foliar N content, N resorption, and NUE c. NUE in ecosystem scale (NUEES) was higher in HA than that of in KS. There was 68.3% of total N productionloss or out from the system inKS. Key words: NUE, N soil content, N resorption, transformation system

Pengaruh Media (Campuran Beras Dan Ampas Tebu) terhadap Pertumbuhan Trichoderma harzianum dan Daya Hambatnya terhadap Fusarium oxysporum secara In vitro Moralita Chatri
Bioscience Vol 2, No 1 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Jamur Fusarium oxysporum merupakan jamur patogen umumnya ditemukan pada tanaman holtikultura. Jamur ini menyebabkan penyakit layu fusarium. Fungisida sintetis merupakan pilihan yang sering digunakan petani untuk menghambat penyebaran jamur ini. Namun penggunaannya menimbulkan banyak dampak negatif bagi lingkungan. Trichoderma harzianum memiliki sifat antagonis yang dapat menggantikan fungisida sintetis dalam menghambat pertumbuhan F. oxysporum. T. harzianum membutuhkan media pembawa seperti beras. Namun membutuhkan biaya yang cukup tinggi dan bersaing dengan kebutuhan manusia. Untuk itu dilakukan campuran antara beras dan ampas tebu untuk mencari media alternatif. Tujuannya agar mendapatkan media tumbuh yang lebih efektif dan efisien.Penelitian ini menggunakan Rancangan Acak Lengkap dengan 5 perlakuan dan 3 ulangan. Perlakuanya terdiri dari A. Media beras 100%, B. Media ampas tebu 100%, C. Media beras dan ampas tebu (1:1), D. Media beras dan ampas tebu (2:1), dan E. Media beras dan ampas tebu (1:2). Paremeter yang diamati yaitu pertumbuhan T. harzianum, kepadatan konidia, dan persentase hambatan terhadap F. oxysporum.Hasil penelitian ini menunjukkan bahwa T. harzianum dapat tumbuh pada campuran media beras dengan ampas tebu. Media tumbuh mempengaruhi pertumbuhan, kepadatan konidia, serta daya hambatnya terhadap F. oxysporum Pertumbuhan yang terbaik yaitu pada media ampas tebu 100 %, kepadatan konidia tertinggi pada media beras 100% (11,6 x 109/ mL) dan persentase daya hambat tertinggi pada media ampas tebu 100% (60%). Hal ini menunjukkan bahwa media alternatif terbaik yaitu media ampas tebu 100%. Keywork : Fusarium oxysporum, layu fusarium, media, Trichoderma harzianum

Ethnobiology exploration of Suku Dayak Losarang, Indramayu District-West Java Province Lesy Luzyawati; Lissa Lissa
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Biodiversity is one of local wisdom that should be kept and maintained. Several local governments have different ways of managing and maintaining their existence, including in Krimun village-Losarang, Indramayu District, West Java Province. This study is aimed at finding the information about Suku Dayak people in Losarang (which is not same as Dayak in Borneo Island), how they manage and utilize biodiversity in their surroundings, and how it relates to the local wisdom. This is a qualitative research with instruments consists of interview sheets, field notes, and audio-visual documentation. The subject of this research is the Suku Losarang Dayak people whom are selected through incidental sampling techniques. The study of Ethnobotany includes utilization of 56 species plants as food, three species as boards, nine species for medicine, two species for aesthetics, and nine species for rituals. Ethnoecology here means the conservation of community of the Suku Dayak Losarang people toward surrounding ecosystems that are conserved for the needs of farming, shelter, and ritual. Concerning for ethnozoology, none of the species was used because they have a principle "ngaji rasa". This principle means that they would not be allowed to consume or to make use of the living creatures.

Effect of Mangosteen Skin Extract (Garcinia mangostana L.) on Males Mice (Mus musculus L. Swiss Webster) Uric Acid Level Rizki Ananda Fitri; Ramadhan Sumarmin; Elsa Yuniarti
Bioscience Vol 1, No 2 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Uric acid (gout) caused by high levels of uric acid in the blood (hyperuricemia). Inflammation caused by uric acid crystals cause pain, stiffness, and swelling in the joints. Gout medication allopurinol commonly consumed ie the mechanism of action as an inhibitor of xanthine oxidase, but this drug side effects such as nausea and diarrhea. The skin of the mangosteen fruit contains xanthones and flavonoids compounds were allegedly able to lower uric acid levels. This study aims to determine the effect of mangosteen peel extract (Garcinia mangostana L.) on uric acid levels in mice (Mus musculus L. Swiss Webster) Males. Research has been conducted in December-January 2016 Biology Laboratory of the Department of Biological Science UNP. This research is experimental method completely randomized design (CRD) ie 4 treatments and 6 replications. Such treatment 0 g / kg (Control / P1), 0.2 g / kg (P2), 0.4 g / kg (P3), 0.6 g / kg (P4). Data were analyzed using ANOVA and using Paired Sample T-Test. Parameters measured were basal uric acid levels before and after treatment. Data taken after 4 days of the treated mice. The results were obtained uric acid levels in mice after treatment are: P1 (Control) 6.43 mg / dL, P2 5.65 mg / dL, P3 3.9 mg / dL, and P4 3.7 mg / dL. Based on the results of this study concluded that the mangosteen rind extract has no effect on uric acid levels in male mice. Keywords: uric acid (gout), xanthine oxidase, hyperuricemia, an antioxidant.

The Effect Of Time Differences On Polyacrilamide Gel Electrophoresis On Cassava DNA Nur Ayu Ramadanti; Dwi Hilda Putri
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

DNA bands formed from the results of electrophoresis with Polyacrilamide gel are considered as 1 character representing 1 allele. PCR products produce multiple bands (multy bands), which indicates that there are multi alleles in the sample. Electrophoresis is a chemical analysis method based on the movement of charged protein molecules in the electric field. Separation is carried out based on differences in the size of the molecular weight and the electric charge contained by the macro-molecule. In addition, the effect of gel concentratio n, buffer and electrophoresis time also has a role in the results of electrophoresis. This study was conducted to compare the best separation time for acrilamide gel electrophoresis with the results of cassava DNA amplification. The materials used in this study are two cassava varieties, namely: Adira IV 1, Adira IV 2, Adira IV 3, Carvita 25 1, Carvita 25 2, and Carvita 25 3. Using electrophoresis by poly-acrilamide gel with two different time effects: 1 hour 30 minutes and 3 hours. The results of electrophoresis with 3 hours gave better results of DNA visualization compared to 1 hour 30 minutes.

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