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Bioscience
ISSN : 2614669X     EISSN : 2579308X     DOI : -
Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.
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Articles 146 Documents
Stability β-carotene encapsulated in plant-based emulsions: impact of nanocrystalline cellulose concentration Ichlasia Ainul Fitri; Thunnalin Winuprasith
Bioscience Vol 6, No 1 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202261116540-0-00

Abstract

ABSTRACT. The influence of nanocrystalline cellulose (NCC) concentrations on stability of 10% of oil-in-water (O/W) emulsions containing β-carotene using tween 20 as an emulsifier was examined. The concentrations of NCC were varied at 0.05, 0.10, and 0.20% (w/w). There was a decrease in particle size (d43) with increasing NCC concentration caused by rod like shape by native NCC. Addition NCC increased viscosity and ζ-potential thereby decreasing rate of creaming index by increasing NCC concentrations. Addition NCC decreased the value of L* due to the white and turbid colour from native NCC but the differences between the emulsions containing NCC and control was not noticeable by visual observation. The stability of β-carotene increased with increasing NCC concentrations. It can be indicated, NCC can be used as natural stabilizer to increase emulsion and b-carotene stability especially at 0.20% (w/w) NCC. This experiment provides useful information for further applications especially CDS for bioactive compound.ABSTRAK. β-carotene memiliki stabilitas rendah selama pengolahan oleh sebab itu perlu diaplikasikan kedalam produk pangan seperti sistem emulsi untuk melindungi β-carotene. Pengaruh konsentrasi nanokristalin selulosa (NCC) terhadap stabilitas 10% emulsi minyak dalam air (O/W) yang mengandung β-carotene dengan tween 20 sebagai emulsifier dianalisis. Konsentrasi NCC divariasi pada 0,05, 0,10, dan 0,20% (b/b). Terjadi penurunan ukuran partikel (d43) dengan meningkatnya konsentrasi NCC yang disebabkan oleh bentuk asli NCC adalah seperti batang pendek dan kecil yang terdispersi sempurna kedalam sistem emulsi. Penambahan NCC meningkatkan viskositas dan ζ-potential dari emulsi sehingga menurunkan laju creaming index pada system emulsi. Penambahan NCC menurunkan nilai L* karena warna putih dan keruh dari NCC asli tetapi perbedaan antara emulsi yang mengandung NCC dan kontrol tidak terlihat secara visual. Stabilitas β-carotene meningkat dengan meningkatnya konsentrasi NCC. Berdasarkan hasil pengamatan dapat diindikasikan bahwa NCC dapat digunakan sebagai stabiliser alami untuk meningkatkan stabilitas emulsi dan β-carotene terutama pada NCC konsentrasi 0,20% (b/b). Percobaan ini memberikan informasi yang berguna untuk aplikasi lebih lanjut terutama untuk enkapsulasi senyawa bioaktif.  
Ecological characteristics of gandaria (Bouea macrophylla Griff) at dungus iwul nature reserve and yan lapa nature reserve Bogor regency Rian Susila; Reni Srimulyaningsih; Sri Wilujeng; Raizal Fahmi
Bioscience Vol 6, No 1 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202261111995-0-00

Abstract

Gandaria (Bouea macrophylla Griff) is the identity flora of West Java Province. Naturally, the gandaria habitat in West Java is found only in the Yan Lapa Nature Reserve (CAYL) and Dungus Iwul Nature Reserve (CADI). In the ecosystem of CADI, and CAYL the position of gandaria in the community shows a low level of dominance. The presence of invasive species in CAYL and CADI is feared will threaten the stability of ecosystems in the region in the future including the presence of native flora. This study aims to study the level of tolerance and adaptation of gandaria in nature by knowing the pattern of individual spread in the gandaria population and the type of gandaria association with other species in various environmental. This is interesting to study because of the limited efforts of species conservation and genetic conservation of gandaria, while the condition of the natural population is increasingly threatened. Individual distribution patterns in gandaria populations in CADI and CAYL are random and clustered. Gandaria at Dungus Iwul Nature Reserve and Yan Lapa Nature Reserve are positively associated with iwul, whereas the Hawuan is negatively associated. Gandaria is not associated with asam keranji, manii, kokopian, leungsir, putat, and teureup. However, gandaria is not with menteng monyet dan kitulang in CADI while in CAYL have positive position.Gandaria (Bouea macrophylla Griff) merupakan flora identitas Provinsi Jawa Barat. Secara alami, habitat gandaria di Jawa Barat hanya terdapat di Cagar Alam Yan Lapa (CAYL) dan Cagar Alam Dungus Iwul (CADI). Dalam ekosistem CADI, dan CAYL posisi gandaria dalam komunitas menunjukkan tingkat dominasi yang rendah. Kehadiran spesies invasif di CAYL dan CADI dikhawatirkan akan mengancam stabilitas ekosistem di kawasan tersebut di masa mendatang termasuk keberadaan flora asli. Penelitian ini bertujuan untuk mempelajari tingkat toleransi dan adaptasi gandaria di alam dengan mengetahui pola penyebaran individu pada populasi gandaria dan jenis asosiasi gandaria dengan spesies lain di berbagai lingkungan. Hal ini menarik untuk dikaji karena terbatasnya upaya konservasi spesies dan konservasi genetik gandaria, sedangkan kondisi populasi alaminya semakin terancam. Pola sebaran individu pada populasi gandaria di CADI dan CAYL bersifat acak dan mengelompok. Gandaria di Cagar Alam Dungus Iwul dan Cagar Alam Yan Lapa berasosiasi positif dengan iwul, sedangkan Hawuan berasosiasi negatif. Gandaria tidak diasosiasikan dengan asam keranji, manii, kokopian, leungsir, putat, dan teureup. Namun gandaria tidak dengan menteng monyet dan kitulang di CADI sedangkan di CAYL memiliki posisi positif.
Prospecting the roles of Trichoderma in sustainable crop production: biotechnological developments and future prospects Rizky Riscahya Pratama Syamsuri; Dwi Astuti Aprilia; Atasya Yasmine Fakhira; Almira Salma Nabilah; Sulistya Ika Akbari; Nia Rossiana; Febri Doni
Bioscience Vol 6, No 2 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202262119346-0-00

Abstract

The filamentous fungal genus Trichoderma are reported to have a significant impact on the growth and development of various crops. Trichoderma species which are residing in the rhizosphere of crops, and as fungal symbionts living within plant tissues have multiple roles in enhancing crops’ agronomic traits, fitness, growth and yield, and in modulating their tolerance towards biotic and abiotic stresses. This article discusses on the potential and impact of Trichoderma in improving the development and production of crops, as well the mechanism of Trichoderma in improving the development and production of crops. This article also highlights the ability of Trichoderma for improving crops’ tolerance to abiotic and biotic stresses. Prospectively, the use of Trichoderma inoculants offers some new, cost-effective, and more eco-friendly practices for increasing crops’ production.
Utilization of organic liquid coffe fertilizer (Coffea arabica L.) as hydroponic nutrition in mustard plants (Brassica juncea L.) Fika Faradhila; Violita Violita; Resti Pevria
Bioscience Vol 6, No 2 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202262109521-0-00

Abstract

As mustard greens contain various benefits that are beneficial to health, because the mustard greens contain vitamins and minerals that play an important role in the process of growth and metabolism of the body. One way to grow vegetables in a narrow area is to use a hydroponic system. Hydroponics is a way of farming without using soil media but uses water as a medium and uses nutrients as nutrients for plant growth. Hydroponic cultivation is one of the efforts to obtain quality agricultural products, healthy, and free of pesticides. However, the use of hydroponic nutrients for now is still sourced from chemical fertilizers. Therefore, one solution to reduce the use of inorganic fertilizers can be used organic fertilizers, namely liquid organic fertilizer which is more easily absorbed by plants because the elements in it have been decomposed. One of the wastes that can be used as liquid organic fertilizer is coffee grounds waste. This study aims to determine the use of liquid organic fertilizer coffee grounds (Coffea arabica L.) as hydroponic nutrients in mustard plants (Brassica juncea L.). This research was conducted from February to April 2020, located at the Integrated Research Laboratory and the wire house of the Department of Biology FMIPA UNP. This study uses a completely randomized design (RAL) with 5 treatments and 3 replications. Data were analyzed using the Analysis of Variance (ANOVA) and DNMRT follow-up tests at the 5% level. The results showed that various concentrations of hydroponic nutrient solution with liquid organic fertilizer based on coffee grounds had a significant effect on increasing the growth of mustard plants (Brassica juncea L.) both from plant height, number of leaves and leaf area. Keywords: Coffea Grounds, Hydroponics, Liquid Organic Fertilizer, Plant SawiSebagai sayuran sawi mengandung berbagai khasiat yang bermanfaat bagi kesehatan, karena pada sayuran sawi mengandung vitamin dan mineral yang berperan penting dalam proses pertumbuhan dan metabolisme tubuh. Salah satu cara untuk penanaman sayuran pada lahan yang sempit adalah dengan menggunakan sistem hidroponik. Hidroponik merupakan cara bercocok tanam tanpa menggunakan media tanah tetapi menggunakan air sebagai media dan menggunakan unsur hara sebagai nutrisi untuk pertumbuhan tanaman. Budidaya secara hidroponik merupakan salah satu upaya untuk memperoleh produk pertanian yang berkualitas, sehat, dan bebas pestisida. Namun penggunaan nutrisi hidroponik untuk saat ini masih bersumber pada pupuk kimia. Oleh karena itu salah satu solusi untuk mengurangi penggunaan pupuk anorganik tersebut dapat digunakan pupuk organik yaitu pupuk organik cair dimana lebih mudah terserap oleh tanaman karena unsur-unsur di dalamnya sudah terurai. Salah satu limbah yang dapat dijadikan pupuk organik cair yaitu limbah ampas kopi. Penelitian ini bertujuan untuk mengetahui pemanfaatan pupuk organik cair ampas kopi (Coffea arabica L.) sebagai nutrisi hidroponik pada tanaman sawi (Brassica juncea L.). Penelitian ini telah dilakukan bulan Februari sampai April 2020, bertempat di Laboratorium Penelitian Terpadu dan rumah kawat Jurusan Biologi FMIPA UNP. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 3 ulangan. Data dianalisis menggunakan uji Analisis Varians (ANOVA) dan uji lanjut DNMRT pada tingkat 5%. Hasil penelitian menunjukkan bahwa berbagai konsentrasi larutan nutrisi hidroponik dengan pupuk organik cair berbahan dasar ampas kopi memberikan pengaruh yang nyata  untuk meningkatkan pertumbuhan tanaman sawi (Brassica juncea L.) baik dari tinggi tanaman, jumlah daun dan luas daun.
Utilization liquid waste of catfish pond as hydroponic nutrition in spinach (Amaranthus hybridus L.) Alifya Putri Diva; Irma Leilani Eka Putri
Bioscience Vol 6, No 2 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202262111834-0-00

Abstract

Catfish farming produces waste products that can be utilized as hydroponic nutrients in plants because they contain nutrients needed by plants. Hydroponics is a method of farming without soil that can be done on limited land. Spinach (Amaranthus hybridus L.) is a type of plant that has high nutrition which can be cultivated hydroponic. The purpose of this study is to determinate the effect liquid waste of catfish pond as hydroponic nutrition in spinach (Amaranthus hybridus L.). The research is experimental study.The study was conducted in February-October 2020, in laboratory and home wire of Biology, FMIPA, Universitas Negeri Padang. This study used a completely randomized design with 5 and 4 replications. Parameters of this study was plant height (cm), number of leaves, leaves area (cm2), wet weight(g), dry weight(g). The data was analized by using ANOVA and BNJ test at 5% level.The results of the study showed that liquid waste of catfish pond is significantly different from the height plants, the number of leaves, leaf width, wet weight and dry weight of spinach (Amaranthus hybridus L.).Budidaya ikan lele menghasilkan limbah yang dapat dimanfaatkan sebagai nutrisi hidroponik pada tanaman karena mengandung nutrisi yang dibutuhkan oleh tanaman. Hidroponik adalah metode bercocok tanam tanpa tanah yang dapat dilakukan pada lahan yang terbatas. Bayam (Amaranthus hybridus L.) merupakan salah satu jenis tanaman yang memiliki nutrisi tinggi yang dapat dibudidayakan secara hidroponik. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh limbah cair kolam lele sebagai nutrisi hidroponik pada bayam (Amaranthus hybridus L.). Penelitian ini merupakan penelitian eksperimen. Penelitian dilaksanakan pada bulan Februari-Oktober 2020, di laboratorium dan home wire Biologi FMIPA Universitas Negeri Padang. Penelitian ini menggunakan rancangan acak lengkap dengan 5 dan 4 ulangan. Parameter penelitian ini adalah tinggi tanaman (cm), jumlah daun, luas daun (cm2), berat basah (g), berat kering (g). Data dianalisis dengan menggunakan ANOVA dan uji BNJ pada taraf 5%. Hasil penelitian menunjukkan bahwa limbah cair tambak lele berbeda nyata terhadap tinggi tanaman, jumlah daun, lebar daun, berat basah dan berat kering bayam. (Amaranthus hybridus L.).
Potential of thermophilic bacteria to produce bioethanol at variation of incubation pH Irdawati Irdawati; Nur Vaizi; Linda Advinda; Dwi Hilda Putri
Bioscience Vol 6, No 2 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202262117426-0-00

Abstract

The purpose of this study was to determine the potency and optimum pH of AAS thermophilic bacteria isolates in producing bioethanol. This research was carried out from September 2021 to December 2021, at the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This research is an experimental study consisting of 6 treatments and 3 replications with variations in incubation pH of 6.5, 7, 7.5, 8, 8.5, and 9. The data obtained were analyzed by means of variance (ANOVA) with Duncan's further test. New Multiple Range Test (DNMRT). The results showed that the isolates of thermophilic bacteria SSA had the potential to produce bioethanol with isolates SSA 8 being the producer of bioethanol with the highest concentration of 1.001%, and variations in incubation pH affected the concentration of bioethanol produced with the optimum pH of thermophilic bacteria in producing bioethanol, namely pH 8 which produced the highest concentration of bioethanol is 1.001%.Tujuan dari penelitian ini adalah untuk mengetahui potensi serta pH optimum isolat bakteri termofilik SSA dalam menghasilkan bioetanol. Penelitian ini dilaksanakan pada bulan September 2021 hingga bulan Desember 2021, di Laboratorium Mikrobiologi  Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Padang. Penelitian ini merupakan penelitian eksperimen yang terdiri dari 6 perlakuan dan 3 ulangan dengan variasi pH inkubasi 6,5, 7, 7,5, 8, 8,5, dan 9. Data yang diperoleh dianalisis dengan sidik ragam (ANOVA) dengan uji lanjut Duncan’s New Multiple Range Test (DNMRT). Hasil penelitian menunjukkan bahwa isolat bakteri termofilik SSA memiliki potensi dalam menghasilkan bioetanol dengan isolat SSA 8 merupakan penghasil bioetanol dengan kadar tertinggi yaitu 1.001%, dan  variasi pH inkubasi berpengaruh terhadap konsentrasi bioetanol yang dihasilkan dengan pH optimum bakteri termofilik dalam menghasilkan bioetanol yaitu pH 8 yang menghasilkan konsentrasi bioetanol tertinggi yaitu 1.001%.
Selection and characterization bacteria in women moslem Husnin Nahry Yarza; Rosi Feirina Ritonga; M. Maryani; H. Handayani
Bioscience Vol 6, No 2 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202262111551-0-00

Abstract

Bacteria are microorganisms that can grow anywhere. Hair and scalp are one of the suitable places for microorganisms to grow and reproduce in women who wear hijab due to the high humidity. The purpose of this study was to isolate and characterization bacteria that grow and reproduce in the hair of women who wear hijab. This research used 32 hair samples from women who were wearing hijab and observed the problems that often occur in the hair of women who wear hijab. The research method used is descriptive. The research got that there were 11 isolated bacteria that grew in the hair and head of women wearing jilbab. Another thing that is observed is the use of the hijab that can be used repeatedly and the basic materials used in the use of the hijab so that it can make you feel comfortable and confident. And the morphological characterization of bacteria was gram negative in IOL1 isolates. From 32 respondents, 11 isolated bacterial grew in the hair of women wearing jilbab and characterization by gram staining on IOL1 isolated, namely gram negative. As many as 53.1% of respondents never used the jilbab repeatedly. Bakteri adalah mikroorganisme yang dapat tumbuh dimana saja. Rambut dan kulit kepala merupakan salah satu tempat yang cocok bagi mikroorganisme untuk tumbuh dan berkembang biak pada wanita berhijab karena kelembapan yang tinggi. Tujuan dari penelitian ini adalah untuk mengisolasi dan mengkarakterisasi bakteri yang tumbuh dan berkembang biak pada rambut wanita berhijab. Penelitian ini menggunakan 32 sampel rambut wanita berhijab dan mengamati permasalahan yang sering terjadi pada rambut wanita berhijab. Metode penelitian yang digunakan adalah deskriptif. Dari hasil penelitian didapatkan bahwa terdapat 11 isolat bakteri yang tumbuh pada rambut dan kepala wanita berjilbab. Hal lain yang diperhatikan adalah penggunaan hijab yang dapat digunakan berulang kali dan bahan dasar yang digunakan dalam penggunaan hijab sehingga dapat membuat anda merasa nyaman dan percaya diri. Dan karakterisasi morfologi bakteri gram negatif pada isolat IOL1. Dari 32 responden, 11 isolat bakteri tumbuh pada rambut wanita berjilbab dan dikarakterisasi dengan pewarnaan gram pada isolat IOL1 yaitu gram negatif. Sebanyak 53,1% responden tidak pernah menggunakan jilbab berulang kali.
Stomata leaves characteristics of sapindaceae family in malabar forest, Malang city Endrik Nurrohman; Roimil Latifa; Samsun Hadi
Bioscience Vol 6, No 2 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202262118189-0-00

Abstract

Malabar forests are composed of various plant families, one of which is the Sapindaceae family. Differences in family, plant species, and environmental factors can affect the number of stomata and the size of plant cells. The purpose of this study was to explore the characteristics (number, density, type, shape, and size) of leaf stomata of plants of the Sapindaceae family in the Malabar forest of Malang City. This type of research is descriptive. The research was conducted in the Malabar City Forest of Malang City and the Biology Laboratory of the University of Muhammadiyah Malang. The study started from March 2021 to September 2021. The sampling technique was carried out using the cruising method. The method of collecting stomatal characteristics data using a light microscope and Scanning Electrone Microscope (SEM). The research data obtained were analyzed by descriptive statistics. The results showed that (1) there were 5 types of plants from the Sapindaceae family, there were variations in the size of the stomata parts of the Sapindaceae (2) family, the longest guard cell length was the stomata of the matoa leaf (22.4 m), the widest guard cell width was the matoa leaf (14.1 m). The longest pore length of rambutan leaves (15.8 m), and the widest pore width of longan leaf stomata (3.88 m), overall stomata are kidney-shaped, stomata types are anomocytic, paracytic, and cyclocytic, the highest stomata density kiara payung leaves (37.44 cells/mm2).Hutan malabar terdiri dari berbagai famili tumbuhan, salah satunya adalah famili Sapindaceae. Perbedaan famili, jenis tumbuhan, dan faktor lingkungan dapat mempengaruhi jumlah stomata dan ukuran sel tumbuhan. Tujuan penelitian ini adalah untuk mengetahui karakteristik (jumlah, kerapatan, jenis, bentuk, dan ukuran) stomata daun tumbuhan famili Sapindaceae di hutan Malabar Kota Malang. Jenis penelitian ini adalah deskriptif. Penelitian dilakukan di Hutan Kota Malabar Kota Malang dan Laboratorium Biologi Universitas Muhammadiyah Malang. Penelitian dimulai pada bulan Maret 2021 hingga September 2021. Teknik pengambilan sampel dilakukan dengan menggunakan metode cruising. Metode pengumpulan data karakteristik stomata menggunakan mikroskop cahaya dan Scanning Electrone Microscope (SEM). Data penelitian yang diperoleh dianalisis dengan statistik deskriptif. Hasil penelitian menunjukkan bahwa (1) terdapat 5 jenis tumbuhan dari famili Sapindaceae, terdapat variasi ukuran bagian stomata dari famili Sapindaceae (2) panjang sel penjaga yang paling panjang adalah stomata daun matoa (22,4 m), lebar sel penjaga terluas adalah daun matoa (14,1 m). Panjang pori terpanjang daun rambutan (15,8 m), dan lebar pori terlebar stomata daun kelengkeng (3,88 m), stomata keseluruhan berbentuk ginjal, tipe stomata anomositik, parasitik, dan siklositik, kerapatan stomata tertinggi daun kiara payung (37,44 sel/mm2).
Soil Fertility Analysis with Soil Microorganism Indicators Febrianti Rosalina; sukmawati sukmawati
Bioscience Vol 6, No 2 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202262116811-0-00

Abstract

Areca nut and sago are typical fruits of the Papuan Community which produce waste. Utilization of waste for the community itself has not been done optimally, especially in agriculture. One way to overcome the problem of soil fertility is by adding organic matter. Areca nut shell waste and sago stem waste are alternative uses of existing waste by processing it into liquid organic fertilizer (POC) and biochar. Adding organic matter to the soil can increase soil productivity and fertility and can affect soil biological properties by increasing the activity of soil biota which play a role in breaking down and decomposing organic matter so that soil biological properties increase. The purpose of this study was to compare the biological properties of the soil applied with biochar and liquid organic fertilizer (POC) derived from areca nut shell and sago bark waste. The research method consisted of a completely randomized design (CRD) which consisted of 7 treatment levels. The first treatment (SB0) was control (without adding biochar), the second treatment (SB1) was areca nut shell waste biochar, the third treatment (SB2) was sago stem waste biochar, the fourth treatment (SB3) was a combination of areca nut shell and stem biochar. sago, the fifth treatment (SB4) was POC of areca nut shell waste, the sixth treatment (SB5) was POC of sago stem waste, and the seventh treatment (SB6) was a combination of POC of areca nut shell waste and sago stems. The parameters of this study included determining the total bacterial population, total fungal population, soil respiration, organic matter activity, nitrogen fixing activity, pH, Organic-C, and Total-N. Based on the results of the research that has been done, it can be concluded that the treatment in the form of biochar and POC in general has a significant effect on increasing soil pH, Organic-C, Total-N, total bacterial population, total fungi population, soil respiration, Nitrogen fixing activity, and The activity of organic matter. Treatment with biochar (SB1, SB2, and SB3) gave the highest average microbial population which was directly proportional to the respiration rate.Pinang dan sagu merupakan buah khas masyarakat Papua yang menghasilkan limbah. Pemanfaatan limbah bagi masyarakat itu sendiri belum dilkakukan secara optimal, terutama di bidang pertanian. Salah satu cara untuk mengatasi masalah kesuburan tanah adalah dengan pemberian bahan organik. Limbah kulit buah pinang dan limbah batang sagu menjadi alternatif pemanfaatan limbah yang ada dengan diolah menjadi pupuk organik cair (POC) dan biochar. Pemberian bahan organik ke dalam tanah dapat meningkatkan produktivitas dan kesuburan tanah serta dapat mempengaruhi sifat biologi tanah dalam meningkatkan aktivitas biota tanah yang berperan dalam merombak serta mendekomposisi bahan organik sehingga sifat biologi tanah meningkat. Tujuan dari penelitian ini adalah untuk membandingkan sifat biologi tanah yang diaplikasikan dengan biochar dan pupuk organic cair (POC) yang berasal dari limbah kulit buah pinang dan kulit batang sagu. Adapun metode penelitian ini terdiri dari Rancangan Acak Lengkap (RAL) yang terdiri dari 7 taraf perlakuan. Perlakuan pertama (SB0) adalah control (tanpa pemberian biochar), perlakuan kedua (SB1) adalah biochar limbah kulit buah pinang, Perlakuan ketiga (SB2) adalah Biochar limbah batang sagu, Perlakuan keempat (SB3) adalah kombinasi Biochar limbah kulit buah pinang dan batang sagu, perlakuan kelima (SB4) adalah POC limbah kulit buah pinang, Perlakuan keenam (SB5) adalah POC limbah batang sagu, dan Perlakuan ketujuh (SB6) adalah kombinasi POC limbah kulit buah pinang dan batang sagu. Parameter dari penelitian ini meliputi penetapan total populasi bakteri, total populasi fungi, respirasi tanah, Aktivitas perombak bahan organic, aktivitas penambat Nitrogen, pH, C-Organik, dan N-Total. Berdasarkan hasil penelitian yang telah dilakukan dapat disimpulkan bahwa pemberian perlakuan berupa biochar dan POC secara umum memberikan pengaruh yang nyata terhadap kenaikan pH tanah, C-Organik, N-Total, total populasi bakteri, total populasi fungi, respirasi tanah, aktivitas penambat Nitrogen, dan Aktivitas perombak bahan organic. Perlakuan dengan pemberian biochar (SB1, SB2, dan SB3) memberikan rata-rata populasi mikroba tertinggi yang berbanding lurus dengan laju respirasinya.
Starter Concentration of SSA4 Thermophilic Bacteria in Producing Xylanase Irdawati Irdawati; Jannah Koftiah; Dwi Hilda Putri; Linda Advinda
Bioscience Vol 7, No 1 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371117423-0-00

Abstract

Advances in the field of biotechnology have led to the use of enzymes in industry increasingly. The high public awareness of environmental pollution makes enzyme technology as an alternative to replace various chemical processes. Enzymes can be used in various industrial sectors such as textiles, food, detergents, paper and cosmetics, as well as biofuels. One of the enzymes that is often used is the enzyme xylanase. Thermophilic microorganisms that can produce thermostable enzymes are thermophilic bacteria. One of the important factors affecting the success of the manufacturing process of the production of the xylanase enzyme is the concentration of the starter. The production of the thermophilic bacterial xylanase enzyme SSA 4 produced the highest specific enzyme activity at a concentration of 4% which was 0.137 U/mg. This shows that low starter concentrations can overwhelm microbial growth well. The increase in the concentration of the starter causes the enzyme activity to decrease, this happens.