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Gilang Nugraha
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INDONESIA
INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Published by Universitas Nahdlatul Ulama Surabaya
ISSN : 26846748     EISSN : 26569825     DOI : https://doi.org/10.33086/ijmlst
Core Subject : Health, Social,
Health Professions Social Sciences
Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument enlargement, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.
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Articles 105 Documents
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The Potential Use of EDTA as an Alternative to Defibrination in Preparing Blood Agar Plates with Human AB Blood Type on Staphylococcus aureus Culture Dora Dayu Rahma Turista; Eka Puspitasari; Fanny Kurnanda Razvi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1923

Abstract

Blood Agar Plates (BAP) are composed of blood as one of the compositions. Sheep’s blood is usually used, but since it is difficult to be obtained, human AB blood type was used as an alternative. In preparing BAP, blood is defibrinated to lyse the blood clotting factors. Blood clots can also be prevented by adding anticoagulants, such as ethylenediaminetetraacetic acid (EDTA). This study aims to investigate the potential use of EDTA as a substitute for defibrination in preparing BAP with human AB blood type. This study employed a completely randomized design with true experimental method using Staphylococcus aureus as the sample. The parameters were the number of colonies, types of hemolysis, and hemolysis zone. The results showed that the S. aureus grown on BAP with EDTA-human AB blood type was 64 colonies (mean), produced β-hemolytic pattern, and 6 mm hemolytic zone. In contrast, the S. aureus grown on BAP with defibrinated human AB blood type showed 82 colonies (mean), β-hemolytic pattern, and 5 mm hemolytic zone. There were significant differences in the number of colonies (0.000 < α) and hemolytic zones (0.02 < α). However, there was no difference in the hemolysis type (both treatments produced β-hemolysis). EDTA was possible to be used as a substitute for defibrination in preparing BAP to assess the hemolysis type of S. aureus, but it might not be able to be used as a benchmark for counting the number of colonies and determining the hemolysis zone of S. aureus.
Liver Histopathological Change and Malondialdehyde Level of Rattus Norvegicus on Administration of Curcuma Zedoaria and Paracetamol Toxic Dose Putu Oky Ari Tania; Puja Ayu Misuari; Satya Yudhayana; Ketut Ayesha Edelwise Prayoga
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1927

Abstract

High doses of paracetamol create necrosis in the liver and produce free radicals. When liver function decreased in a long time, it will lead to severe liver damage and it will be irreversible. Rhizome of Curcuma zedoaria has the potential effect as an antioxidant, it is assumed that  its properties inhibit the formation of free radicals which formed from toxic doses of paracetamol. The aim of this study was to examine the histological structure of the liver and to determine MDA levels in the administration of C. zedoaria toxic dose and paracetamol on the Rattus norvegicus. The study was used twenty-four rats divided into four groups (positive control: CMC 0.5%; negative control: paracetamol 1.35g/kg body weight; treatment group 1 (T1): C. zedoaria 105 mg/200g and paracetamol 1.35g/kg Body weight 2 hours later, and treatment group 2 (T2): paracetamol 1.35g/ kg Body weight and C. zedoaria 105 mg/200g 2 hours later).  The Kruskall-Wallis test results showed MDA level did not significantly different between groups (p = 0.087). Hepatocellular changes were observed descriptively with Hematoxylin-eosin staining. Positive control showed greater hepatocellular changes rather than other groups, hepatocyte cells were enlarged with cytoplasm showing eosinophilic granules infiltrates, enlarged irregular nuclei, nucleolus prominent, there are many necrosis cells.
RETRACTION NOTICE TO ‘‘N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) in Stage 1 and Stage 2 Hypertension Patients” [Ina. J. Med. Lab. Sci. Tech. 2019;1(2): 64-67] Supriati Wila Djami
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.2061

Abstract

This article has been retracted at the request of the Editor in Chief of the Indonesian Journal of Medical Laboratoy Science and Technology (IJMLST) volume 1 number 2 of 2020 pages 65-67 with DOI: 10.33086/ijmlst.v1i2.1159 because it has been published in the Journal of Health Information volume 17 number 1 year 2019 pages 64-74 with DOI: 10.31965/infokes.Vol17.Iss1.232. Papers submitted to IJMLST should neither published previously nor be under consideration for publication another journal. Therefore, the author should make a statement upon submitting. Reusing data in any form must follow good citation principles, therefore this paper has ethical violations in the scientific publishing system.
Modified Spin Column-Based RNA Extraction Methods of Staphylococcus aureus using PureLink® RNA Mini Kit and Basic Laboratory Instrument Muhammad Taufiq Hidayat; Endry Nugroho Prasetyo
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i2.1863

Abstract

RNA extraction is an important process before gene expression assessment at the transcriptomic level. RNA is a sensitive material to environmental factors such as temperature and contaminants, so the RNA extraction process generally requires sophisticated and expensive laboratory instruments. In this study, we extract RNA from Staphylococcus aureus bacteria using the PureLink® RNA Mini Kit. The instruments used in this study are basic instruments such as a hand homogenizer and non-thermal centrifuge. The results of RNA extraction were visualized using agarose gel electrophoresis. These results indicate that bacterial RNA extraction can be performed using the PureLink® RNA Mini Kit even with inexpensive basic laboratory instruments.
Electrolytes Levels (Na, K, Cl) in Serum Stored at 4°C Temperature Amalia Nurul Fauziah; M. Atik Martsiningsih; Budi Setiawan
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i2.1870

Abstract

The samples used for serum electrolyte measurement should be analyzed immediately after being received in the laboratory within 1-2 hours to avoid an increase in the error of the results. Serum should be stored at 4°C for a period to prevent damage. The analyst should consider maximum delay time in the examination to maintain the serum's quality. This study compared the 2-hour and 3-hour delays in sodium (Na), potassium (K), and chlorine (Cl) tests. The method used in this study is an observational analysis with a cross-sectional study design. The samples in this study used 35 patient serum residues. The study was conducted in November 2020 with a continuous sampling technique. Electrolyte levels in the sample were measured by AVL 9180 Electrolyte Analyzer using Ion-Selective Electrode (ISE) method. The differences in electrolyte (Na, K, Cl) levels were analyzed by the Kruskal-Wallis Statistical test at a 95% confidence level. The results showed that the content of sodium, potassium, and chlorine were 0.719; 0.976; and 0.772. This study showed that there was no significant difference in the electrolyte content of sodium (Na), potassium (K), and chlorine (Cl) in the serum directly detected from the serum stored at 4°C for 2 hours and 3 hours. In conclusion, it is acceptable to postpone the serum test for 3 hours with various considerations.
The Relationship Between Leukocytes Numbers and Consciousness Level of Craniotomy Patients at The Jemursari Islamic Hospital Surabaya in 2018-2019 Ainul Rofik; Utami Ambarsari; Hafizh Auliyan Sodali; Misbakhul Munir
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i2.1889

Abstract

This study investigates the correlation between leukocyte number and the patient's awareness level after craniotomy surgery, both high (leukocytosis) and low (leukopenia). Several studies reveal that an increase in leukocytes affects mortality rates due to the high level of leukocytes affecting our body's functionalities, including awareness. Previous studies revealed that a leukocyte count exceeding 17.5x106/L was associated with a lower GCS score, a longer hospital stay, and worsening CT scan results, regardless of the type of focal lesion that occurred. This study uses observational methods in the form of retrospective case studies. The data was collected from the medical records of the Operation room at Jemursari Islamic Hospital in 2018-2019 with a sample size of 89. The research was conducted at Jemursari Islamic Hospital Surabaya from May 2018 to August 2019. Data were analyzed using the Spearman correlation test with p <0.05. Results showed no significant relationship between the leukocyte count and the consciousness level of post-craniotomy patients. Future research could estimate the specific effects on the morphology of specific leukocyte cells that are elevated in post-craniotomy patients. This research is expected to provide insight into the effect of leukocytes on consciousness to reduce patient mortality after craniotomy.
Differences of Spermatozoa Concentration Analysis Between Manual and Automatic Methods Emma Ismawatie; Purwanto Adhipireno; Seso Sulijaya Suyono; Edy Purwanto; Budi Santoso; Edward Kurnia Setiawan Limijadi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i2.1961

Abstract

The examination of sperm concentration in the laboratory is the calculation of the number and motility using a microscope or using a device. There are still some clinicians who doubt the accuracy of the sperm count results using a semen analyzer rather than using the manual method. This study aims is to determine the differences of the sperm concentration examination between the manual method and the automatic method. Subjects in this study were patients who carried out semen analysis tests at the Clinical Pathology Laboratory of RSIA "Restu Ibu" Sragen from June to August 2020. The object of this research is the examination of sperm concentration, using a manual method using a hemocytometer and an automatic method using the LensHooke ™ SQA X1 Pro. The results of statistical tests using the Mann Whitney methods show that the significance value (p) was 0.960, which means that there was no difference in the results of  the sperm concentration examination between the manual method and the automatic method. Result of this research shows that there is no weakness or significant difference if compared between manual and automatic methods.
Pigeonpea (Cajanus cajan L.) and Cowpea (Vigna unguiculata L.) Water as Alternative Growth Medium for Escherichia coli and Staphylococcus aureus in Laboratory with Minimum Infrastructure Norma Kambuno; Ninda P. Y. Amtaran; Sherly Dewu; Kuntum E. Nurdin; Ni Made Susilawati; Yoan Novicadlitha
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i2.2076

Abstract

The availability of non-synthetic media from natural ingredients is needed to answer the needs in laboratories where the price of nutrient media is quite expensive and there are limited supplies of material ware houses. Cowpea (Vigna unguiculata L.) and pigeonpea (Cajanus cajan L.) are the local foods of people of NTT (East Nusa Tenggara) which have a high enough nutritional content which has the potential to be developed into cheap, easy and simple non-synthetic media in making. The purpose of this study was to determine whether the agar media contained nutrient from cowpea and pigeonpea water can be used as a alternative for nutrient agar for the growth of Escherchia coli and Staphylococcus aureus bacteria. This research is a true experiment with posttest-only control design. The growth rate of S. aureus bacteria on pigeonpea medium, cowpea medium, nutrient agar medium, were 164 CFU/mL (SD=3,13), 161 CFU/mL (SD=3,02) and 164 CFU/mL (SD=3,21), respectively. The average growth of E. coli on cowpea medium, pigeonpea medium, and nutrient agar control medium were 163 CFU/mL (SD=2,79), 167 CFU/mL (SD-2,63) and 164 CFU/mL (SD=2,75) respectively. Test results ANOVA between pigeonpea medium, cowpea medium and nutrient medium in order to obtain p value = 0.145 (p> 0.05) for the growth of E. coli bacteria and p value = 0.393 (p> 0.05) for growth S. aureus. It was concluded that there was no difference between the number of bacterial colonies of E. coli and S. aureus on three medium. The pigeonpea medium and cowpea can be used to grow and alternative nutrient agar in order to grow bacteria E. coli and S. aureus.
Assessment of Platelet Indices Profile of Pregnant Women Attending University of Abuja Teaching Hospital, Nigeria Amos Dangana; Anthony Uchenna Emeribe; Hezekiah Alkali Isah; Sanusi Musa; Joel Monday Abu; Solomon Oloche Onoja; Nkechi Onukegbe; Idris Nasir Abdullahi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i2.2110

Abstract

Platelets initiate hemostasis by aggregating at the site of injury and participate in ensuring endothelial integrity. A defect in this process could lead to intravascular blood loss. This case-control study sought to determine the platelet counts and indices among pregnant women in the University of Abuja Teaching Hospital Gwagwalada, Abuja, Nigeria. A total of 120 pregnant women as case and 60 non-pregnant women as control were enrolled for this study. Blood samples were collected in EDTA tubes, and complete platelet count and indices were carried out using an automated five-part haematology analyzer. The mean ± standard deviation of the platelets count among the pregnant women, 226.54 ± 69.76 109 cells/L was not significantly different from that of the non-pregnant women, 214.95 ± 52.22x 109 cells/L (p=0.295). There was a significant differences in mean platelets volume (MPV) of the case and control groups (p=0.036). After post-hoc test, the significant difference was between the  pregnant women in 3rd trimester and the control group (p=0.014). However, there was no diffences in the mena platelets larger cell ratio and platelet distribution width in the case and control groups. Fifteen (11.0%) and 7 (12.1%) of the case and control control groups, respectively had mild thrombocytopenia. However, there was no significant association between pregnant status and thrombocytopenia (p=0.836). Based on these findngs, it can be infered that platelet count and MPV decreases while PDW increase with the progression of gestation age compared to the non-pregnant women.
Factors Related to Soil Transmitted Helminth Infection in Vegetable Farmers Ririh Jatmi Wikandari; Lilik Setyowatiningsih; Masrifan Djamil; Surati Surati; Fitriani Kahar
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i2.2145

Abstract

Soil Transmitted Helminth (STH) infection can occur at all ages, from children to the elderly. Farmers are at risk of contracting STH infection because of their daily work which is in direct contact with the soil. The risk of STH infection is due to poor personal hygiene and environmental sanitation. The study aims to determine observe the factors associated with STH infection in vegetable farmers, using a cross-sectional approach. The research subjects were vegetable farmers in Batur Wetan Hamlet, Getasan, Semarang Regency. Data were collected using a questionnaire to determine the personal hygiene and sanitation hygiene of farmers when working in the garden. Worm identification used (reference,) based on the worm performance using the floating method. Worm identification were done by examining the stool using the floating method. The results of the study were processed using SPSS version 20. Data analysis using Chi-Square (α = 5%), found 3 positive study subjects infected with STH, consisting of 1 egg of Ascaris lubricoides and 2 eggs of Trichuris trichiura. Positive STH respondents, have a washing by water and soap habits before eating and defecating, without wearing gloves. There was a relationship between hand washing by water and soap habits before eating to worm infection, but no relationship between this habit after defecating and no wearing gloves to worm infection. Vegetable farmers are advised to change the daily habits and maintain personal hygiene after working. use personal protective equipment when working and maintain personal hygiene.

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