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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
 103   104   105   106   107 
Omentin-1 Levels in Obesity Putri Hidayasyah Purnama Lestari; Nurahmi Nurahmi; Tenri Esa; Liong Boy Kurniawan
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1737

Abstract

Omentin-1 is an anti-inflammatory adipokine secreted by stromal vascular cells. Inflammation and apoptosis of adipocyte tissue in obesity lead to decreased production of omentin-1. This study aims to find the difference in omentin-1 levels in the obese and non-obese groups and the correlation between levels of omentin-1 with BMI and waist circumference. This study was a cross-sectional study involving 70 subjects with 37 people in the obese group and 33 people in the non-obese group. Anthropometric data including weight, height, waist circumference, and BMI were measured. Serum omentin-1 levels were measured by ELISA. The statistical tests used were Chi-square, T-test, Mann-Whitney test, and Spearman test. Test results were significant if p-value < 0.05. Significant difference was observed in serum omentin-1 levels between obese and non-obese group (median 140,31 range (88.08 – 382.76) vs. 210,97 range (124,44–577,96) ng/mL), respectively; p < 0.001). Serum omentin-1 correlated negatively with BMI (p=0.001, r = - 0.398) and waist circumference (p=0.017, r = - 0.286). Obesity causes inflammation and increased death of adipocyte tissues due to apoptosis, autophagy, and fibrosis resulting in decreased production of omentin-1 by stromal vascular cells. Anthropometric parameters of waist circumference and BMI describe the distribution of adipocyte tissue and affect the secretion of omentin-1. Omentin-1 levels in the obese group were lower than in the non-obese group. The higher the BMI and waist circumference, the lower the omentin-1 level.
Analysis of Neutrophil Lymphocyte Ratio and Absolute Lymphocyte Count as Predictors of Severity of COVID-19 Patients Yunianingsih Selanno; Yuyun Widaningsih; Tenri Esa; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 27 No. 2 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i2.1738

Abstract

It is important to predict the severity of COVID-19 during the pandemic. Both Neutrophil Lymphocyte Ratio (NLR) and Absolute Lymphocyte Count (ALC) are two easy, low-cost, and fast inflammatory markers, which positively correlate with the severity of COVID-19. The purpose of this research was to analyze the value of NLR and ALC as predictors of COVID-19 severity. This research was a retrospective study using medical record data of 376 COVID-19 patients during April-September 2020 at the Hasanuddin University Hospital and Makassar City Regional Hospital. Patients were classified into non-severe and severe COVID-19. Neutrophil lymphocyte ratio and ALC values were determined based on routine blood test (Sysmex XS-800i) results, statistical analysis using Independent T-test, while NLR and ALC diagnostic values were analyzed with Receiver Operating Characteristics (ROC) curve to obtain the cut-off value, p < 0.05 was significant. The samples consisted of 372 non-severe and 49 severe COVID-19 patients. Neutrophil lymphocyte ratio value in non-severe (4.02±5.22) was significantly different from severe COVID-19 (9.81±7.06) (p < 0.001), similar to ALC in non-severe (2.00±0.83x103/μL) and severe COVID-19 (1.22±0.78x103/μL) (p < 0.001). Receiver operating characteristics curve showed that NLR had a sensitivity of 91.8% and specificity of 66.4% with a cut-off ≥ 3.17 with Negative Predict Value (NPV) of 98.2% and Positive Predict Value (PPV) of 29.0%; while ALC had a sensitivity of 81.6% and specificity of 64.8% at cut-off ≤ 1.74x103/μL with NPV of 95.9% and PPV of 25.8%. Increased NLR and decreased ALC in severe COVID-19 patients occurred due to an increased inflammatory response resulting in a decreased cellular immunity. Receiver operating characteristics curve showed a cut-off for NLR of 3.17 and ALC of 1.74x103/μL, indicating an optimum sensitivity and specificity. It was concluded that NLR and ALC can be used as predictors of COVID-19 severity with a cut-off ≥ 3.17 and ≤ 1.74x103/μL, respectively.
The Relationship of Neutrophil-Lymphocyte Ratio and Glycemic Control in Type 2 Diabetes Mellitus Patients Nurahmi Nurahmi; Budi Mulyono; Windarwati Windarwati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1739

Abstract

Progressivity of type 2 Diabetes Mellitus (DM) is associated with a condition of chronic inflammation. The Neutrophil-Lymphocyte Ratio (NLR) has become a potential new marker of inflammation to detect chronic inflammation. This research aimed to determine NLR differences between controlled type 2 DM and uncontrolled type 2 DM groups. This research conducted an observational with a cross-sectional approach to 56 patients with type 2 diabetes. The identity, anthropometric measurements, and laboratory data of routine blood exam and HbA1c were carried out on each research subject, and then the NLR calculations were performed. The subjects were 20 (35.70%) controlled type 2 DM patients, 36 (64.30%) uncontrolled type 2 DM patients, consisting of 36 (64.30%) male and 20 (35.70%) females. The NLR value was statistically significantly higher in uncontrolled type 2 DM patients than controlled type 2 DM patients, which was 1.90±0.84 compared to 1.52±0.50 (p=0.035). There was a significant difference in the NLR value between the uncontrolled type 2 DM group and the controlled type 2 DM group.
Relationship between Atherogenic Index of Plasma with HbA1c Levels in Type 2 Diabetes Mellitus Patients Ni Putu Sukma Sumantri Prabandari; Ida Ayu Putri Wirawati; Ni Nyoman Mahartini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1743

Abstract

Type 2 Diabetes Mellitus (T2DM) is a global health problem due to an increasing prevalence and incidence. HbA1c as a parameter for glycemic control is still above the desired target of 7%. Atherogenic Index of Plasma (AIP) is predicted to be an indicator of cardiovascular disease risk in T2DM. Dyslipidemia in T2DM patients showed a result of increased triglyceride and decreased HDL cholesterol levels. This study aimed to determine the relationship between HbA1c and AIP, triglycerides, and HDL cholesterol in T2DM patients. An analytical observational study using a cross-sectional method on 74 DMT2 patients who underwent HbA1c and lipid profile examinations in January-March 2020. Atherogenic index of plasma was calculated by the logarithmic equation (triglycerides/HDL cholesterol). Data were analyzed by SPSS 25.0. The relationship between HbA1c with AIP, triglycerides, and HDL cholesterol using the Pearson correlation test. Atherogenic index of plasma (0.25±0.25) and triglycerides (211.92±146.09 mg/dL) were found to be higher in the poor glycemic control group (HbA1c> 7%) than AIP (-0.04±0.20) and triglycerides (108.96±38.96 mg/dL) in the good glycemic control group (p < 0.05). HDL cholesterol (40.08±12.64 mg/dL) was found to be higher in the poor glycemic control group than HDL cholesterol (52.28±18.12 mg/dL) in the good glycemic control group (p < 0.05). There was a significant positive correlation between HbA1c and AIP (r=0.411, p=0.000), HbA1c with triglycerides (r=0.418, p=0.000), and a significant negative correlation between HbA1c and HDL cholesterol (r=-0.233, p=0.046). Insulin resistance can cause lipid metabolism disorders, inflammation, oxidative stress, and coagulation disorders. Maintaining glycemic control and lipid control plays an important role in preventing diabetes complications. There is a significant positive correlation between HbA1c and AIP, HbA1c and triglycerides, and a significant negative correlation between HbA1c and total cholesterol in T2DM patients.
Correlation between Very Low-Density Lipoprotein and Trygliseride with Glycated Hemoglobin Levels in Type 2 Diabetes Mellitus Patients Sherly Karolina Simanjuntak; I Nyoman Wande; Ida Ayu Putri Wirawati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1744

Abstract

Patients with Type 2 Diabetes Mellitus (T2DM) have an increased prevalence of dyslipidemia, which contributes to a higher risk of dyslipidemia- related complications in T2DM such as cardiovascular disease and stroke. This study aimed to determine the correlation between TG and VLDL-C towards HbA1c levels in a person with T2DM. A retrospective study of 74 outpatients with T2DM at Sanglah General Hospital, Denpasar, who examined serum HbA1c and lipid profiles were traced for serum TG. From the obtained TG profile, a secondary calculation of VLDL was carried out using the Friedewald equation (TG/5). A correlation test was used to determine the relationship between TG and VLDL-C towards HbA1c levels. Serum TG (212.95±147.46 mg/dL) and VLDL (36.69±23.54 mg/dL) were found to be higher in the group with poor glycemic control (HbA1c > 7 mg/dL) compared to serum TG (111.00±39.56 mg/dL) and VLDL (21.05±6.13 mg/dL) in the group with good glycemic control (HbA1c ≤ 7 mg/dL) (p < 0.05). A positive correlation between serum TG (r=0.512; p < 0.001) and VLDL (r=0.18; p <0.001) towards HbA1c levels in T2DM patient was found. Insulin resistance increases the production of VLDL and ApoC-III in the liver and increased chylomicron absorption in the gastrointestinal tract, causing prolonged post-prandial lipemia and disruption of VLDL and TG clearance, thereby resulting in increased TG and VLDL in circulation. There is a significant positive correlation between serum TG and VLDL towards HbA1c levels in a patient with T2DM.  
Graves Disease (Thyroid Storm) with Polyautoimmune Disorders (Autoimmune Hemolytic Anemia and Probable Autoimmune Hepatitis) Mabruratussania Maherdika; Banundari Rachmawati; Andreas Arie Setiawan
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1745

Abstract

Graves' disease is caused by IgG antibodies that bind to the Thyroid Stimulating Hormone (TSH) receptor on the surface of the thyroid gland. These bonds drive the growth of stimulated thyroid follicular cells causing the glands to enlarge and increase the production of thyroid hormones. Previous studies mention the association of HLA-B8 and HLA-DR3 with Graves' disease and the Cytotoxic T-lymphocyte-associated-4 (CTLA-4) gene on chromosome 2q33 as a result of reducing T-cell regulation, resulting in autoimmune disease. Autoimmune thyroid disease is often found together with other autoimmune disorders (polyautoimmune). A 51-year-old male complained of dyspnea, yellowing of the body, and a lump on the neck. One year ago, he was diagnosed with hyperthyroidism. Graves' disease was suspected due to a score of 22 for the Wayne index, FT4 96.9 pmol/L, TSHs <0.01 μIU/mL, TRAb 10.8 IU/L, thyroid uptake test for toxic diffuse struma. In addition, the patient had atrial fibrillation and a thyroid storm with a Bruch Wartofsky index score of 65. Laboratory examination found normocytic normochromic anemia, thrombocytopenia, reticulocytosis, direct coomb test and auto control results positive one, SGOT 87 U/L, SGPT 59 U/L, alkali phosphatase 166 U/L, total bilirubin 38.13 mg/dL, directbilirubin 16.59 mg/dL, indirect bilirubin 21.54, LDH 318 U/L, establishing the diagnosis of Autoimmune Hemolytic Anemia (AIHA). Autoimmune hepatitis score: 15, so a diagnosis of probable autoimmune hepatitis was made.
Determining Acute Leukemia Lineage Using Mie Map Red Blood Cell Nelly Zuroidah; Arifoel Hajat; Paulus Budiono Notopuro
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1747

Abstract

The determination of myeloid and lymphoid lineage is essential for the diagnosis and therapy of acute leukemia. Immunophenotyping is the gold standard to determine the lineage of acute leukemia, but it is still constrained and relatively expensive. Mie Map RBC in the ADVIA 2120i is a parameter that can give additional information about myeloid and lymphoid lineage but has never been studied before. It is expected that Mie Map RBC can be used to differentiate the lineage of acute myeloid and lymphoid leukemia if immunophenotyping is not present. This study aimed to analyze the diagnostic value of Mie Map RBC with ADVIA 2120i towards immunophenotyping in determining myeloid and lymphoid lineage in acute leukemia. Child and adult patients diagnosed with acute leukemia (n=30) that had peripheral blood smear and bone marrow aspiration with blasts > 20% were examined using ADVIA 2120i. The Mie Map RBC lineage results were compared to the lineage of immunophenotyping. The sensitivity and specificity of the Mie Map RBC myeloid series are respectively 60.00%, 93.33%. The sensitivity and specificity of the Mie Map RBC lymphoid series are respectively 93.33% and 60.00%. The diagnostic accuracy value of Mie Map RBC is 76.67%. The determination of acute leukemia myeloid series lineage has high specificity. If there is no population outside the matrix of Mie Map RBC, it highly suggests myeloid series. On the other hand, the determination of acute leukemia lymphoid series lineage has a relatively low specificity meaning that the population outside the matrix of Mie Map RBC does not always suggest a lymphoid lineage
Correlation of Nitrite Oxide with Severity and Survival Rate of Sepsis Patients Sotianingsih Sotianingsih; Budi Mulyono; Andaru Dahesihdewi; Samsirun Halim; Ahmad Syauqi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1749

Abstract

The objective of this research was to determine the correlation between Nitric Oxide (NO) levels with the severity of sepsis, to describe the kinetics of NO levels, and to evaluate it in predicting mortality. This research was a longitudinal cohort observational analytical study. The variables were serum NO levels and SOFA scores, which were serially evaluated. The correlation test and difference test were used for statistical analysis. The survivor and the non-survivor group consisted of 14 (41.18%) and 20 (58.82%) patients, respectively. There was a correlation between serum NO levels and the SOFA score at the 24-hour observation (r=0.403; p=0.041). Non-parametric Mann-Whitney test showed that there was no kinetics of NO th levels at 0, 24, 72, and 144-hour observation (p-values =0.897 and 0.703, respectively). NO levels > 111,16 μmol/L at the 24 hour could predict the risk of death with hazard ratio 4.7 compared to NO levels < 111,16 μmol/L. The survival rate of patients with serum NO levels <111,16 μmol/L and > 111,16 μmol/L was 83.3% and 37.5%, respectively. There was a correlation between serum NO levels and SOFA scores at the 24-hour observation. However, there was no kinetics of NO levels at serial evaluations. Nitric oxide levels with a cut-off of 111,16 μmol/L at 24 hours could predict the survival of septic patients. Utilization of serum NO level at 24 hour can be used to evaluate the severity of septic patients and aggressive management if there is an increase in serum NO levels > 111,16 μmol/L at 24 hours.
Correlation between Platelet to Lymphocyte Ratio with C-Reactive Protein in COVID-19 Patients Novianti Anggie Lestari; Dwi Retnoningrum
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1750

Abstract

Coronavirus 2019 (COVID-19) is an infectious disease caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Inflammation occurs when the body is infected with the virus. Platelets play a role in immune response and immunomodulation by activating P-Selectin Glycoprotein (PSGL) to the site of inflammation. Lymphocytes play a role through CD4 T-cells, B-cells producing specific viral antibodies, and CD8 cytotoxic T-cells by directly killing the virus in infected cells. This study aimed to prove the correlation between PLR and CRP as inflammation markers in COVID-19 patients. This study was a retrospective observational study with the cross-sectional approach at Dr. Kariadi Hospital, Semarang, for the period March-August 2020. Spearman test performed for analyzing data with p<0.05 was significant. Thirty-three confirmed COVID-19 patients with median value of PLR 218 (103-1609) and CRP 15.94 (1.24-200) mg/L were tested for correlation with a value of p=0.013 and r=0.427. The increase of PLR and CRP in COVID-19 patients was caused by an inflammatory process mediated by the immune response. High values in the blood were associated with disease severity and poor prognosis. There was a statistically significant moderate positive correlation between PLR and CRP in COVID-19 patients.
Analysis of Blood Availability During the COVID-19 Pandemic Period in Blood Bank Dr. Wahidin Sudirohusodo Hospital Antariksa Putra; Raehana Samad; Sri Julyani; Rachmawati Adiputri Muhiddin
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1752

Abstract

Blood Bank's challenge during the COVID-19 era is securing and protecting blood supplies even though countries are taking precautionary measures with social distancing to prevent or reduce the number of infections caused by COVID-19. This study aimed to compare blood availability before and during the COVID-19 pandemic at the blood bank of Dr. Wahidin Sudirohusodo Hospital. A Descriptive-analytic study with an observational approach using the Shapiro-Wilk test to determine the normality of the sample and the paired T-test. Sample data was taken between March-August 2019 and March-August 2020. A significant difference was found in blood demand (p-value=0.004), amount of blood transfusion(p-value=0.006), stock and reference report (p-value=0.005), blood service report (p-value=0.005), cito waiting time (p-value=0.002) and regular waiting time (p-value=0.016). There was no significant difference in blood indicator Packed Red Cell (PRC) (p-value=0.119). The Large-Scale Social Restriction Policy (PSBB) and reduction of elective surgery in hospitals affect the fulfillment of Blood Bank and faster attendance time of blood during the pandemic. The decrease in a number of blood demands during the COVID-19 pandemic affected the number of blood transfusions, blood service reports, stocks, referrals, and cito and regular waiting time services.

Page 105 of 133 | Total Record : 1328

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