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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
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Diagnostic Test of Serum Pregnancy-Associated Plasma Protein-A Level as Biomarker for Early Diagnosis of Acute Myocardial Infarction Novida Dwi Astuti; JB. Suparyatmo; Amiroh Kurniati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 3 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i3.1578

Abstract

Acute coronary syndrome is the primary cause of death from heart disease worldwide. This syndrome is caused by ruptured/eroded coronary atherosclerotic plaque, resulting in partial/total occlusion of thrombosis. It is necessary to find novel cardiac biomarkers for the identification of plaque progression before ischemic and myocardial necrosis events. Pregnancy Associated Plasma Protein-A (PAPP-A) is an atherosclerotic mediator proven to be a biomarker for plaque instability. This study aimed to determine the performance of serum PAPP-A as a biomarker for the early diagnosis of AMI. This research was an analytical observational study with a cross-sectional approach. Serum PAPP-A was measured using enzyme-linked immunosorbent assay in 82 new patients. They had ACS and were admitted to the emergency installation of Dr. Moewardi Hospital in Surakarta in August-September 2019. The subjects were grouped into the AMI group (NSTEMI and STEMI) consisting of 49(59.8%) subjects and non-AMI (UAP) group composed of 33(40.2%) subjects based on ACS diagnostic criteria of PERKI 2018. Receiver Operator Characteristic (ROC) curve analysis showed that PAPP-A was a good discriminator between AMI and non-AMI patients. The area under the curve was 0.968, 95% CI (0.932–1.004), with a sensitivity of 91.8% and specificity of 90.9% (p< 0.05). The cut-off value from the ROC curve was 2,526 ng/mL. Serum PAPP-A level has excellent performance as a biomarker for early diagnosis of AMI. It can also function as a screening instrument for the identification of UAP cases developing into AMI.
The Thyroid Stimulating Hormone and Free Thyroxine Levels in Correlation with Serum Bilirubin in Neonatal Jaundice Elvira Dwijayanti; MI. Diah Pramudianti; Dian Ariningrum
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 27, No 1 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i1.1607

Abstract

Congenital hypothyroidism is known to cause prolonged hyperbilirubinemia in neonates. It also correlates with delayedmaturation of the activity of the uridine diphosphate glucoronosyltransferase (UDPG-T) enzyme. Thus, this study wasperformed to analyze the correlation of TSH and FT4 levels with serum bilirubin in neonatal jaundice. This observationalanalytical study with a cross-sectional approach was conducted on 64 neonatal patients with jaundice in Dr. MoewardiGeneral Hospital, Surakarta during September-November 2019. The data comparison and correlation were analyzed withMann-Whitney and the Spearman test. A p-value of < 0.05 was considered significant with 95% Confidence Interval (CI). Thestudy variables comprised of total bilirubin of 12.7 (6.28-23.5) mg/dL, direct bilirubin of 0.8 (0.30-6.61) mg/dL, indirectbilirubin of 11.87 (3.16-22.94) mg/dL, TSH of 4.4 (0.40-23.06) uIU/L, and FT4 of 22.85±7.4 pmol/L. The TSH and FT4 weremoderately correlated with total bilirubin r=-0.444; p=0.001 and r= -0.467; p=0.001), with indirect bilirubin (r= -0.3362;p=0.03 and r=-0.411; p=0.001) and with direct bilirubin (r= -0.257; p=0.040 and r=0.232; p=0.065), respectively. A moderatecorrelation of TSH and FT4 with total and indirect bilirubin, as well as a weak correlation between TSH and direct bilirubinwere found, while no correlation was found between FT4 and with direct bilirubin. Thyroid function screening isrecommended in neonates with jaundice, due to the importance of thyroid hormones in the function and formation oforgans                    
Comparison of Hepcidin Levels in Children with and without Soil-Transmitted Helminths Infection Dewi Saputri; Yunilda Andriyani; Almaycano Ginting
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1471

Abstract

Helminths infection is one of the diseases that still occur insociety. The helminth infection caused by theSoil-Transmitted Helminths (STHs) group, which is Ascaris lumbricoides, Trichuris trichiura, and hookworm in human cancause chronic bleeding resulting in decreasir on storage in the body and increased level of hepcidin. Hepcidin is a liverhormone which regulates iron metabolism and can function as marker of inflammation and iron deficiency. This study aimedto compare the hepcidin levels in STH-infected and non-infected children. A cross-sectional study was conducted betweenMay and October 2018 on 28 STH infected and 140 non-infected subjects. The collected stool samples were analyzed usingthe Kato-Katz method to determine the presence of STH infection and the degree of infection. Urine samples wereprocessed, and their hepcidin levels were measured using a Sandwich-ELISA method. Measurement was made using aSpectrophotometer. The difference of numeric variables was analyzed using Wilcoxon test. The prevalence of STH infectionwas 16.66%. The prevalence of Trichuris trichiura 10.71%, Ascaris lumbricoides 4.76% and hookworm 2.97%. The prevalenceof a single infection was 14.88% and mixed infection 1.78%. Based on the intensity of infection, 15.48% of subjects were mildinfection, 0.59% moderate infection, and 0.59% severe infection. Hepcidin levels in the infected and uninfected group didnot differ significantly (p=0.978). There were no different hepcidin levels in children with and without soil-transmittedhelminths infection.
Diagnostic Performance of Serum (1,3) β-D Glucan to Detect Fungal Infection in Acute Leukemia Patients with Chemotherapy Dian Ariani Wirastuti; B. Rina A Sidharta; Yuwono Hadi Suparto; Leli Saptawati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 27, No 1 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i1.1598

Abstract

Chemotherapy is a predisposing factor for infection in patients with malignancy, while culture, as the gold standard,limits the diagnosis of fungal infections. (1,3) β-D glucans, the most abundant polysaccharide component of the fungal wall,are increased in patients with Invasive Fungal Infections (IFI). This research was an analytical observational study with across-sectional approach involving 60 acute leukemia patients who received chemotherapy with suspicion of fungalinfection at the General Hospital of Dr. Moewardi, Surakarta, from September to October 2019. Fungal blood cultures andserum (1,3) β-D glucan levels by the enzyme-linked immunoassay method were examined. Diagnostic tests were performedto determine sensitivity, specificity, Positive Predict Value (PPV), Negative Predict Value (NPV), Positive Likehood Ratio (PLR),Negative Likehood Ratio (NLR), and the serum's accuracy value (1,3) β-D glucan levels to fungal culture. Most (88.3%) ofpatients were diagnosed with Acute Lymphocytic Leukemia (ALL), maintenance chemotherapy phase (51.3%), risk factorsfor neutropenia (50%), and intravenous (IV) line use (56.7%). Serum (1,3) β-D glucan levels in patients with positive fungalcultures (4) in blood samples had a median of 482.87 (476.13-640.56) pg/mL, while patients with negative fungal cultures(56) had a mean±SD 298,68±114,39 pg/mL. Diagnostic test with a cut-off of 471,717 pg/mL showed sensitivity of 100.0%,specificity of 96.4%, NPV of 100%, PLR of 28.00, and NLR of 0.00 with an Area Under Curve (AUC) value of 0.982 andCoefficient Interval (CI) 95% (0.950-1.014). The measurement of serum (1,3) β-D glucan at a cut-off value of 471,717 pg/mLshowed good performance as a biomarker for diagnosing and screening IFIs. 
DIAGNOSTIC VALUE OF FASTSURE TB DNA RAPID TEST FOR DIAGNOSIS OF PULMONARY TUBERCULOSIS Diyan Wahyu Kurniasari; Jusak Nugraha; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1203

Abstract

Diagnosis tuberkulosis (TB) di Indonesia menurut Kemenkes RI 2014 masih mengandalkan pemeriksaan mikroskopis Basil TahanAsam (BTA) dari hapusan dahak namun memiliki kepekaan dan kekhasan diagnostik yang rendah. Kepekaan diagnostik kulturMycobacterium tuberculosis (M.tuberculosis) pada media Lowenstein Jensen (LJ) lebih tinggi daripada mikroskopis BTA namun hasilnyamemerlukan waktu 6-8 minggu. Uji cepat Fastsure TB DNA menggunakan metode Cross Priming Amplification (CPA) merupakanuji deteksi kualitatif DNA M.tuberculosis yang diamplifikasi pada satu suhu tetap dan menggunakan nucleic acid lateral flow stripdalam perangkat plastik tertutup. Penelitian ini dilakukan untuk menilai nilai diagnostik uji cepat Fastsure TB DNA untuk diagnosistuberkulosis paru. Metode penelitian secara observasional potong lintang selama Juni sampai November 2015. Total 58 spesimen dahakdari 33 orang terduga tuberkulosis paru dan 25 orang non-tuberkulosis dari RS Paru Karang Tembok Surabaya. Setiap spesimendilakukan pemeriksaan mikroskopis BTA, kultur M.tuberculosis pada media LJ sebagai standar baku emas dan ekstraksi DNA danamplifikasi pada uji cepat Fastsure TB DNA. DNA terekstraksi dimasukkan ke dalam tabung kemudian ke cartridge yang tersedia dalamperangkat. Hasil diamati dalam waktu 30 menit berupa munculnya garis uji dan pembanding pada pita. Berdasarkan penelitian ini,diperoleh kepekaan dan kekhasan diagnostik uji cepat Fastsure TB DNA masing-masing sebesar 84,8% dan 92% dengan koefisien kappaadalah 0,757 menunjukkan kesesuaian yang cukup baik. Uji cepat Fastsure TB DNA merupakan pemeriksaan yang cepat, praktis, relatiftidak mahal untuk diagnosis M.tuberculosis dari spesimen klinis khususnya pada BTA negatif.
THE ANALYSIS OF CALCIUM LEVEL IN STORED PACKED RED CELLS Suryani Jamal; Rachmawati Muhiddin; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1194

Abstract

Penelitian ini bertujuan mengetahui; pengaruh penyimpanan terhadap kadar kalsium pada darah simpan Packed Red Cells (PRC);menentukan kadar kalsium pada hari ke-7, hari ke-21 dan hari ke-35 dan membandingkan kadar kalsium hari ke-7 dan hari ke-21,hari ke-7 dan hari ke-35, hari ke-21 dan hari ke-35. Penelitian ini dilaksanakan di Bank Darah dan Laboratorium Patologi Klinik RSUPDr. Wahidin Sudirohusodo. Metode yang digunakan dalam penelitian ini adalah observasional dengan pendekatan potong lintang.Pengambilan sampel dilakukan di semua darah simpan Packed Red Cells (PRC) yang disalurkan di Bank Darah RSUP Dr. WahidinSudirohusodo. Data dianalisis dengan uji statistik Repeated Anova Test. Hasil penelitian menunjukkan bahwa terdapat perbedaanbermakna kadar kalsium darah simpan PRC akibat pengaruh penyimpanan pada hari ke-7 dan hari ke-21, pada hari ke-7 dan harike-35 serta hari ke-21 dan hari ke-35. Penurunan kadar kalsium terjadi karena eritrosit sudah mulai terjadi lisis.
PLATELET LEUCOCYTE AGGREGATES ANALYSIS IN LEUCODEPLETED AND NON-LEUCODEPLETED PLATELET CONCENTRATES Teguh Triyono; Raehanul Bahraen
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 3 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i3.1458

Abstract

Activated platelet could initiate aggregation and linkage with nearby leucocytes to form Platelet-Leucocyte Aggregates (PLA). Leucodepletion procedure could remove leucocyte and separate it from the other blood components therefore minimalizing the probability of PLA formation. We analyze percentage difference of PLA in leucodepleted and non-leucodepleted platelet concentrate. Dual expression of CD41 and CD45 was determined by flowcytometry method representing the value of PLA, PLA percentage of each group was calculated and analyzed with statistical software SPSS 22. Mean percentage value of PLA in leucodepleted group was 63.05 ±19.86, meanwhile in non-leucodepleted group was 64.61 ±17.27. We found that the percentage of PLA in nonleucodepleted group is higher than leucodepleted although the difference is not statistically significant.
THE AGREEMENT BETWEEN LIGHT CRITERIA AND SERUM ASCITES ALBUMIN GRADIENT FOR DISTINGUISHING TRANSUDATE AND EXUDATE Rike Puspasari; Lillah Lillah; Efrida Efrida
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1135

Abstract

Jenis cairan asites transudat atau eksudat perlu dibedakan sebagai tahap awal untuk mengetahui penyebab penyakit yang mendasariasites. Penggabungan beberapa tolok ukur memiliki kepekaan dan kekhasan yang baik dalam membedakan jenis cairan asites. Penelitianini bertujuan untuk mengetahui kesesuaian antara patokan Light dan Serum Ascites Albumin Gradient (SAAG) dalam membedakantransudat dan eksudat pada cairan asites. Penelitian ini merupakan penelitian analitik dengan desain potong lintang terhadap 24 pasienasites di RSUP Dr. M. Djamil Padang, mulai bulan Maret sampai September 2016. Tolok ukur yang diperiksa adalah protein jumlahkeseluruhan (metode kolorimetrik biuret), albumin (metode kolorimetrik bromocresol green), serta laktat dehidrogenase (LDH) (metodeenzimatik). Hasil pemeriksaan setiap tolok ukur dirumuskan kedalam patokan Light dan SAAG. Kesesuaian patokan Light dan SAAGdalam membedakan transudat dan eksudat cairan asites ditentukan dengan uji kappa. Hasil dianggap bermakna secara statistik jikap<0,05. Ciri subjek penelitian ini adalah laki-laki sebanyak 54,2% dan perempuan 45,8% dengan rentang umur 22–76 tahun. PatokanLight dapat menentukan 9 eksudat dan 15 transudat, sedangkan menggunakan SAAG dapat menentukan 2 eksudat dan 22 transudat.Kesesuaian patokan Light dengan SAAG menggunakan uji kappa adalah cukup (nilai kappa=0,26) dan tidak bermakna secara statistik(p>0,05). Hasil penelitian ini menyimpulkan tidak terdapat kesesuaian antara patokan Light dan SAAG dalam membedakan transudatdan eksudat pada cairan asites. Penelitian dalam jumlah besar perlu dilakukan untuk menentukan kepekaan dan kekhasan keduapemeriksaan.
Comparison of 25-Hydroxyvitamin D Levels in Pediatric Hematologic Cancer with and without Suspected Sepsis Erfina Lim; IGAA Putri Sri Rejeki; I Dewa Gede Ugrasena
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 27, No 2 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i2.1632

Abstract

Vitamin D is known to play an important role in fighting infections through innate and adaptive immune systems. Children with cancer have a higher risk of suffering sepsis and vitamin D deficiency. Some studies on vitamin D levels in pediatric cancer with sepsis have shown varied results and remain controversial. This study aimed to analyze 25-hydroxyvitamin D levels in pediatric hematologic cancer with and without suspected sepsis. The study was an observational analytical study with a cross-sectional design. Samples were collected during April–September 2019 from the Pediatrics Outpatient Clinic of the Dr. Soetomo Hospital, Surabaya. The samples were a group of pediatric hematologic cancer consisted of 32 subjects with suspected sepsis and 30 without sepsis. Each group was measured the 25-hydroxyvitamin D levels, then differences in levels of 25-hydroxyvitamin D between groups were assessed. Vitamin D levels were measured using ADVIA Centaur. Both groups had low 25-hydroxyvitamin D levels with a deficiency state of 96.9% and 80% in the suspected sepsis group and group without sepsis. There were differences in levels of 25-hydroxyvitamin D in pediatric cancer with and without suspected sepsis (p=0.045). Lower vitamin D levels were found in the cancer group with suspected sepsis. Low vitamin D levels reduce T-helper 2 activity, decrease cellular immunity, and decrease phagocytic macrophages, leading to an easier entry of pathogens and bacteremia. There were significant differences in 25-hydroxyvitamin D levels in pediatric hematologic cancer with and without suspected sepsis.
Analysis of Smear Microscopy and Culture Conversion Results in Multidrug-Resistant Tuberculosis Patients with and without Type 2 Diabetes Mellitus Henny Fauziah; Aprianti S; Handayani I; Kadir NA
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 3 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i3.1590

Abstract

  The World Health Organization (WHO) recommended microscopic AFB smear examination and culture as follow-ups to the response of MDR TB therapy. Analyzed the results of microscopic AFB smear and culture conversion as well as treatment outcome in Multidrug-Resistant Tuberculosis (MDR-TB) patients with and without Diabetes Mellitus (DM). This is a retrospective study involved 70 MDR-TB patients with (27 patients) with DM and without DM (43 patients) who had microscopic AFB smear and culture results at the start of the follow-up therapy. This research was conducted at Labuang Baji Regional Public Hospital, Makassar, from June to July 2019, used medical records of MDR-TB patients the period of June 2016 to December 2017. The results showed that 52 out of 70 MDR-TB patients had microscopic AFB smear and culture conversion in MDR-TB with DM (21 patients) and without DM (31 patients). The duration of microscopic AFB smear conversion in MDR TB patients with DM (3.33±0.54 months) was longer than patients without DM (2.07±0.05 months), p=0.001. While in culture conversion, there was no significant difference between MDR-TB with DM (1.28±0.64 months) and without DM (1.25±0.59), p=0.648. The recovery outcome between MDR-TB with (48.1%) and without DM (48.8%) was not significantly different. However, the output of treatment failure was greater in DM (11.2%) than without DM (2.3%), although statistically, there was no significant difference (p=0.568). Multidrug-resistant tuberculosis patients with DM experienced slower microscopic AFB smear conversion than MDR-TB patients without DM. However, in culture, there was no significant difference in the conversion period between the two groups. MDR-TB patients, both of with and without DM, had the same chance of recovery.

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