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Jaringan Laboratorium Medis
ISSN : -     EISSN : 26858495     DOI : -
Core Subject : Health, Science,
Jurnal Online Jaringan Laboratorium Medis adalah peer-reviewed journal yang mempublikasikan artikel-artikel ilmiah tentang ilmu kesehatan yang meliputi bidang Analis Kesehatan yang meliputi Penelitian Bakteriologi, Kimia Klinik, Parasitologi, Toksikologi Klinik, Hematologi, Sitohistoteknologi dan Imunologi, Teknologi Bank Darah. Dengan e-ISSN: 2685-8495 diterbitkan oleh Jurusan Analis Kesehatan Politeknik Kesehatan Kemenkes Semarang, terbit 6 bulan sekali, pada bulan Mei dan November setiap tahunnya.
Articles 270 Documents
Kadar Asam Urat Pada Lansia Peserta Senam di Posyandu Lansia Pangalissani, Fransiska
Jaringan Laboratorium Medis Vol. 4 No. 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8485

Abstract

Process of getting older causes a person to experience physical and mental, spiritual, economic and social changes as well as degeneration of organ function. Age is one of the risk factors for high uric acid. Uric acid is the final product or waste product from purine metabolism, that excess amount in the blood will crystallize. At an advanced age, there will be a decrease in the production of several enzymes and hormones in the body that play a role in the process of removing gout. Exercise such as elderly gymnastic regularly can delay physiological changes that usually occur in a decrease in strength and flexibility, as well as an increased susceptibility to injury. The results are presented in descriptive form which supported by tables to describe uric acid levels and its causative factors in elderly gymnastic participants at the elderly posyandu in Village Timang, Sub District Wonokerto, District Wonogiri. Examination of uric acid levels from 73 respondents were 42 respondents (57.5%) with normal uric acid levels and 31 respondents (42.5%) with uric acid levels above normal. An increase in uric acid levels can be caused by several factors such as age, sex, food intake, physical activity and history.
Validasi Jarak pada Pneumatic Tube System terhadap Hasil Pemeriksaan Trombosit (PLT) dan Kalium Setyaji, Yoki; Novitasari, Tri; Muktianafi, Inggil Tunjung; Anggreini, Shintia Dini; Palupi, Norma Agustin
Jaringan Laboratorium Medis Vol. 4 No. 1 (2022): May 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i1.8488

Abstract

Pneumatic tube system (PTS) is a sample transport medium that is widely used in hospitals. Samples sent using PTS may be shaken due to the varying speed and air pressure in the PTS. The use of PTS can affect the examination of platelet counts and potassium levels. The installation of PTS at the Gadjah Mada University Academic Hospital was carried out in May 2020 in the Yudistira, Arjuna, Kresna and Nakula buildings. Delivery of laboratory samples at RSA UGM every day can be done from buildings with different distances. Every hospital that uses PTS is advised to validate PTS and investigate blood specimens for the possibility of hemolysis and laboratory results. The purpose of this study was to validate the PTS distance as a transport medium for laboratory examinations. This research is an experimental research which is a follow-up study using 30 samples. Samples were taken twice and sent with the PTS from two different distances, namely YD 2 and IGD. The statistical test will use a paired sample t-test. The data will be analyzed with the help of Prima Graph 8 Statistical Software with a significance level of 5%. The results of the analysis showed that there was no difference in the results of the examination of platelets and potassium sent from YD 2 or from the ER.
Analisis Risiko Kadar Enzim SGOT dan SGPT pada Penderita Diabetes Mellitus Tipe 2 Maulana, Mochamad Rizal; Kuswarini, Sutji
Jaringan Laboratorium Medis Vol. 4 No. 1 (2022): May 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i1.8489

Abstract

Diabetes mellitus is a group of metabolic diseases that occur due to abnormalities in insulin secretion and/or insulin action. In type 2 diabetes mellitus there is a decrease in glucose absorption by the body due to insulin resistance. Energy metabolism from glucose is processed through the synthesis of fatty acids in the liver (cytosol). The process that occurs continuously causes the accumulation of lipids in the liver or "fatty liver" so it is necessary to check the levels of transaminase enzymes which include SGOT and SGPT. The purpose of this study was to analyze the levels of SGOT and SGPT enzymes in patients with type 2 diabetes mellitus using the analytical observational method. The results of the examination of SGOT and SGPT levels from 30 patients who were taken randomly at Dr. Soetomo Surabaya will be analyzed with bar graphs and diagrams. Of 30 random samples, 20 samples (66.67%) had SGOT levels above the normal value, and 12 samples (40%) had SGPT with results above normal values. This study concludes that there is an increase in the levels of SGOT and SGPT enzymes in patients with type 2 diabetes mellitus.
Perbandingan Berbagai Metode Pengecatan Spora pada Bacillus Cereus Widodo, Widodo; Purlinda, Devi Etivia; Riadi, Ahmad
Jaringan Laboratorium Medis Vol. 4 No. 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8493

Abstract

Endospores are formed by members of several genera of gram-positive bacteria, such as Bacillus and Clostridium. The spores are very resistant structurally; withstand the temperature of boiling water for two hours or more. They contain very little water and show very few chemical reactions. The favorable external environment will cause the spores to break down and the vegetative cells to appear to grow and reproduce. Identification of endospore-forming pathogens is important in food and medical microbiology. However, the spores contain multiple protective layers, which cannot be easily penetrated by simple staining or Gram staining techniques. Therefore, it is necessary to apply heat to help penetrate the color, with various methods of Gram stain, fluorescent spore painting, Schaeffer and Fulton spore painting and Client's spore painting method. The results of the fluorescent painting method have better results but require much more expensive equipment using a fluorescent microscope considering the facilities that must be provided for the Schaeffer and Fulton painting method as an alternative if they do not have these facilities.
Skrining Hipertensi pada Pendonor Darah di Kabupaten Rembang Putri, Adita Puspitasari Swastya; Ayu Kartika, Astri Dewi
Jaringan Laboratorium Medis Vol. 4 No. 1 (2022): May 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i1.8496

Abstract

Hypertension is one of the non-communicable diseases that is the cause of death in the world. WHO estimates the prevalence of hypertension is 22% of the total world population. Patients with hypertension often do not realize that they have hypertension and only find out after experiencing complications, so health screening is necessary. Blood donation in Rembang Regency also check their blood pressure before donating their blood. This can be a positive effort to improve health, control and manage signs and symptoms that arise. The purpose of this study was to screening blood donation for hypertension in Rembang Regency. This research is an observational study with a cross sectional design. The population in this study were voluntary blood donation who donated their blood during the month of Ramadhan at PMI Rembang Regency in 2018-2020 with a total sample of 260 blood donations. The sampling technique used in this research is simple random sampling. Based on data from blood donation in Rembang Regency, the results showed that most of the blood donation were male, as many as 221 (85.0%) blood donations and most of them aged 25-34 years, namely 81 (31.2%) blood donations. Most of the blood donation were included in the pre-hypertension category as many as 226 (86.0%) blood donations, and there were 12 (4.6%) blood donations included in the hypertension I category.
Profil Respon Time Pelayanan Darah di PMI Kabupaten Kuningan Salsabila, Zhaika; Sri Supadmi, Francisca Romana; Rahardian Syah, Deby Zulkarnain
Jaringan Laboratorium Medis Vol. 4 No. 1 (2022): May 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i1.8501

Abstract

One tangible manifestation of the provision of public services in the health sector is the Blood Transfusion Unit (UTD) which provides blood services. Blood Transfusion Services are health service efforts that include planning, mobilizing and preserving blood donors, supplying blood, distributing blood, and providing medical treatment to patients for the purpose of healing illness and restoring health. The Indonesian Red Cross, hereinafter abbreviated as PMI, is a social organization whose main tasks and functions are in the field of kepalangmerahan based on statutory provisions. Efforts to meet the availability of blood for the needs of health services so far have been made by PMI through UTD spread throughout Indonesia based on assignments by the Government In chapter II article 2 paragraph 1 PMK Number 83 of 2014 concerning blood transfusion units, hospital blood banks, and service networks blood transfusion is stated that: UTD is only held by the Government, Regional Government, or PMI. This research is a type of descriptive quantitative research. The approach used is cross sectional. The average blood service time at PMI Kuningan Regency in 2020 is 1 hour and can be completed in a minimum time of 35 minutes and a maximum of 3 hours 57 minutes. The stages that contribute to the length of blood service time are in the post-analytic stage and factors that influence the service time in the Kuningan Regency PMI laboratory, namely, the number of officers, length of service and facilities.
Profil Hasil Pemeriksaan Dahak Pada Pasien Multidrug-Resistant Tuberculosis (MDR-TB) Nadza, Alisa Khodrun; Surati, Surati
Jaringan Laboratorium Medis Vol. 4 No. 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8526

Abstract

TB patients who do not adhere to OAT treatment can lead to resistance and become MDR-TB and add to the burden of TB management globally. TB control programs including microscopic sputum examination are important for diagnosis, evaluation, follow-up treatment, and follow-up. The results of the sputum examination used the IUATLD scale as an interpretation of the results and the reporting was negative, scanty, positive one, positive two, and positive three according to the number of TB germs found. The results of the sputum examination to determine the classification of the patient, the decision to start treatment, and the patient's recovery. At the step of treatment for MDR-TB patients, the results of sputum examination are an indicator to determine the success rate of treatment which is marked by the conversion of BTA. Research objectives is to determine the description of the results of sputum examination in Multidrug-Resistant Tuberculosis (MDR-TB) patients at the Kendal District Health Center. Research methods is observational research with descriptive research criteria through a cross sectional approach. Respondents of the study were multidrug-resistant tuberculosis (MDR-TB) patients from three health centers in Kendal district with the highest case prevalence. The study respondents were 7 MDR-TB patients. All study respondents, totaling 7 MDR-TB patients (100%), were included in the advanced step of treatment and showed negative sputum examination results. All study respondents (100%) showed that the results of microscopic sputum examination were negative, which means that BTA conversion occurred at the advanced treatment step.
Gambaran Kepositifan Basil Tahan Asam Pasien Diagnosis Klinis Tuberculosis Paru di Balai Kesehatan Masyarakat Semarang Kahar, Fitriani; Novitasari, Putri Anggraeni; Irnawati, Irnawati
Jaringan Laboratorium Medis Vol. 4 No. 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8552

Abstract

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Until now, tuberculosis is still a public health problem in the world. Tuberculosis is a dangerous infectious disease, the healing process also takes a long time. Acid-fast bacilli examination is an examination to determine the presence or absence of Mycobacterium tuberculosis bacteria. The factors that influence Tuberculosis patients are age, gender, education and occupation. The purpose is to knowing the description of AFB positivity in patients with clinical diagnosis of pulmonary tuberculosis at Balkesmas Semarang in 2020. This research was an observational study with descriptive research criteria through a retrospective approach. Results from 656 patients with clinical diagnosis of tuberculosis who underwent acid-fast bacilli examination, 220 patients (33.5%) were found positive and acid-fast bacilli were negative (64.5%). The results of the examination of acid-fast bacilli were positive with a scanty positivity degree of 3 patients (1.4%), acid-fast bacilli (+1) were 71 patients (32.3%), (+2) were 83 patients (37.7%), (+3) as many as 63 patients (28.6%). Based on the category of age 0-14 years as many as 1 patient (0.5%), 15-34 years as many as 78 patients (35.5%), 35-54 years as many as 96 patients (43.6%), 55-74 years as many as 44 patients (20%), >75 years as many as 1 patient (0.5%). Based on gender, there were 123 patients (56%) male and 97 female patients (44%). Conclusion the results of the examination of acid-fast bacilli were positive with a scanty positivity rate of 3 patients (1.4%), acid-fast bacilli (+1) 71 patients (32.3%), (+2) 83 patients (37.7%), (+3) 63 patients (28.5%). Based on gender with positive acid-fast bacilli, there were 123 male patients (56%) while female 97 patients (44%). Age with the most positive acid-fast bacilli results in the range 0–14 years 1 patient (0.5%), 15–34 years 78 patients (35.5%), 35-54 years 96 patients (43.6%), 55 –74 years.
Pengaruh Lama Penyimpanan Jamu Beras Kencur terhadap Pertumbuhan Kapang Pinto Laksono, Fransiskus Xaverius Agung; Dwiyanti, Ratih Dewi; Rifqoh, Rifqoh; Insana, Aima
Jaringan Laboratorium Medis Vol. 4 No. 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8571

Abstract

During the Covid-19 pandemic, people began to maintain their health, by using herbal medicine as an alternative treatment. One type of herbal medicine used is jamu beras kencur because it has properties to increase stamina, increase appetite, and relieve aches and cough disorders. In the Regulation of the Minister of Health of the Republic of Indonesia No. 007 of 2012 carrying herbs such as jamu beras kencur does not require marketing authorization. The quality of the herbal medicine produced has not been confirmed as feasible from microbial contamination, especially in molds, the number of yeast molds in the jamu beras kencur must comply with BPOM standards Number 32 of 2019 namely AKK <103 colonies/mL. This study aimed to determine the effect of the storage time of jamu beras kencur on mold growth with storage treatments of 0 hours, 4 hours, 8 hours, 12 hours, and 24 hours at room temperature. This type of research is a Quasi Experiment with a research design of One Group Pretest-Postest Design. The results of this study showed that the average AKK value was 0 hours 0.2x100 colonies/mL, 4 hours 0.4x100 colonies/mL, 8 hours 0.4x100 colonies/mL. 12 hours 0.8x100 colonies/mL and 24 hours 2.2x100 colonies/mL. These results stated that the jamu beras kencur met the BPOM standard requirements Number 32 of 2019 which was AKK <103 Colonies/mL. The results of the Kruskal Wallis test obtained a sig value of 0.286, it was concluded that there was no effect of storage time for the jamu beras kencur on the growth of molds. The identification results on the jamu beras kencur obtained fungal species, namely Aspergillus flavus, Aspergillus niger, and Penicillium sp. Suggestions for further researchers can examine the effect of storage time on mold growth in other types of herbal medicine.
Uji Efektivitas Air Perasan Daun Jeruk Limau Kuit ( Citrus hystrix ) Sebagai Insektisida Nabati Terhadap Mortalitas Kutu Rambut Pediculus humanus capitis Secara In Vitro Pusvita, Nidha; Thuraidah, Anny; Rifqoh, Rifqoh; Rakhmina, Dinna
Jaringan Laboratorium Medis Vol. 4 No. 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8579

Abstract

Lemongrass leaves are a typical plant of South Kalimantan which contain anti-insecticide, namely secondary metabolites such as alkaloids, saponins, tannins, steroids and triterpenoids. Pediculosis is a disease caused by a tick infection which is still neglected and becomes a health problem. One way to get rid of head lice is to use natural ingredients that contain anti-insecticide substances. This study aims to examine the possibility of using natural ingredients in lime leaves as vegetable insecticides against head lice mortality. The research method used is experimental, with a research design that is a post-test only control group, which measures and compares the mortality variable of Pediculus humanus capitis after being treated with lime leaf juice with concentrations of 10%, 30%, 60%, 100% during 1, 6, 12 and 24 hours and compared them with the negative control group with aquadest and the positive control with pemethrin. The sample of this study was lime leaf extract which was extracted by the method of squeezing using water. From the results of the study obtained the highest percentage of death at 12 and 24 hours with 100% mortality at a concentration of 100%. Based on the results of statistical tests using mortality observation data, the LC99 values were obtained within 1 hour (197.678%), 6 hours (126.055%), 12 hours (89.847%), and 24 hours (60.758%). Based on the results of the analysis of the data obtained, it can be concluded that there is effectiveness of lime leaf juice (Citrus hystrix) on the mortality of head lice (Pediculus humanus capitis) in vitro. Then the juice of lime leaves can be used as an alternative to getting rid of head lice with natural ingredients.