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Jaringan Laboratorium Medis
Published by Poltekkes Kemenkes Semarang
ISSN : -     EISSN : 26858495     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Public Health
Jurnal Online Jaringan Laboratorium Medis adalah peer-reviewed journal yang mempublikasikan artikel-artikel ilmiah tentang ilmu kesehatan yang meliputi bidang Analis Kesehatan yang meliputi Penelitian Bakteriologi, Kimia Klinik, Parasitologi, Toksikologi Klinik, Hematologi, Sitohistoteknologi dan Imunologi, Teknologi Bank Darah. Dengan e-ISSN: 2685-8495 diterbitkan oleh Jurusan Analis Kesehatan Politeknik Kesehatan Kemenkes Semarang, terbit 6 bulan sekali, pada bulan Mei dan November setiap tahunnya.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 295 Documents
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Penggunaan Minyak Kayu Putih Sebagai Agen Clearing Pengganti Xylol Pada Pewarnaan Hematoksilin-Eosin (HE) Preparat Histologi Hepar Mencit Deswita Aulia Pristanti; Juliana Christyaningsih; Ratno Tri Utomo; Christ Kartika Rahyuningsih
Jaringan Laboratorium Medis Vol 8 No 2 (2026): November 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i2.15447

Abstract

Xylene is a commonly used clearing agent in histological slide preparation; however, its toxic properties necessitate the development of safer alternative materials. One potential alternative is eucalyptus oil (Melaleuca cajuputi). This study aimed to analyze the potential of eucalyptus oil as an alternative clearing agent to replace xylene based on the quality of mouse liver (Mus musculus) histological slides stained with Hematoxylin-Eosin (HE). This experimental study was conducted from October 2025 to May 2026 at PUSVETMA Surabaya, the Faculty of Veterinary Medicine, Universitas Airlangga, and the Cytology and Histotechnology Laboratory of Poltekkes Kemenkes Surabaya. A total of 32 mouse liver histological slides were divided into two groups, consisting of 16 slides cleared using xylene and 16 slides cleared using eucalyptus oil. Slide quality was assessed based on nuclear characteristics, cytoplasm, intercellular boundaries, and color uniformity using a scoring system ranging from 1 to 3. Data were analyzed using the Shapiro-Wilk test, followed by the Kruskal-Wallis and Mann-Whitney tests. The results showed that the mean quality score was 11.13 in the xylene group and 9.44 in the eucalyptus oil group, with a mean difference of 1.69 points. The Mann-Whitney test indicated a significant difference in slide quality between the two groups (p < 0.001). Eucalyptus oil was able to produce histological slides categorized as good, although its quality remained lower than that of xylene as the gold standard. Therefore, eucalyptus oil has the potential to be used as a safer and more environmentally friendly alternative clearing agent.
Karakteristik Antibodi Ireguler pada Pasien Gagal Ginjal Kronik di Laboratorium Rujukan UDD PMI Kota Semarang Iin Desmiany Duri; Hilari Rio Rosa; S.Y Didik Widiyanto; Putri Wulandari; Mutia Syafira; Rina Puspita
Jaringan Laboratorium Medis Vol 8 No 2 (2026): November 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i2.15541

Abstract

Patients with Chronic Kidney Disease (CKD) undergoing maintenance hemodialysis are highly susceptible to severe anemia and frequently require repeated long-term blood transfusions. This therapeutic intervention exposes patients to non-ABO foreign red blood cell antigens, potentially triggering alloimmunization through the formation of irregular antibodies. These antibodies may compromise transfusion safety and complicate compatibility testing (crossmatching) in transfusion laboratories. This study aimed to determine the prevalence and specificity of irregular antibodies among CKD patients at the Referral Laboratory of the Blood Donor Unit, Indonesian Red Cross (PMI) Semarang City. This descriptive quantitative study analyzed secondary laboratory records from 55 CKD patients collected between July 2020 and September 2025. Immunohematological data included ABO and Rh blood grouping, antibody screening using the Indirect Antiglobulin Test (IAT), Direct Antiglobulin Test (DAT), and antibody identification using a 10-cell red blood cell panel. The results showed that most participants were aged 40–60 years (50.9%), with blood group O being the most prevalent (36.4%). Of the total samples, 17 patients (30.9%) tested positive for irregular antibody screening. Further antibody identification detected Anti-M (MNS blood group system) in two cases (11.8%) and Anti-e (Rh blood group system) in two cases (11.8%). The majority of positive cases (13 patients; 76.4%) demonstrated non-specific antibody reactions. Most non-specific results are thought to be associated with the potential presence of autoantibodies, particularly in patients with a positive Direct Antiglobulin Test (DAT). Patients with chronic kidney disease (CKD) face a relatively high risk of developing irregular antibodies. This condition creates a masking effect that conceals other alloantibodies and leads to major incompatibility during crossmatching. Therefore, it is recommended that reference laboratories routinely employ adsorption techniques to ensure the safety of blood for transfusion.
Hubungan Antara Variasi Konsentrasi Dan Waktu Fiksasi Neutral Buffer Formalin Terhadap Intensitas Pewarnaan Hematoksilin Eosin Pada Preparat Histologi Wafiq Vanisa Auliasari; Yuyun Nailufar; Yeni Rahmawati
Jaringan Laboratorium Medis Vol 8 No 2 (2026): November 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i2.15624

Abstract

Fixation is a crucial step in preparing histological specimens and has a significant impact on the quality of the tissue to be examined under a microscope. The use of Neutral Buffer Formalin (NBF) at inappropriate concentrations or for improper fixation times can affect the quality of Hematoxylin and Eosin (HE) staining results. This study aims to determine the effect of variations in NBF concentration and fixation time on the intensity and quality of HE staining results using mouse colon tissue (Mus musculus). This study is a quasi-experimental study with a descriptive quantitative approach. The study samples consisted of mouse colon tissue subjected to four fixation treatments at room temperature: 5% NBF for 48 hours, 10% NBF for 24 hours, 15% NBF for 16 hours, and 20% NBF for 12 hours. The results of this study were scores analyzed using the Shapiro-Wilk test, and a significance value of 0.004 (≤0.05) was obtained, indicating that the research data were not normally distributed; the analysis was then continued with the Kruskal-Wallis test. Based on the results of the Kruskal-Wallis test, an Asymp. Sig. value of 1.000 (≥0.05) was obtained, indicating no significant difference in histological staining quality among the variations in concentration and fixation time of Neutral Buffer Formalin.
Gambaran Anemia pada Ibu Hamil Trimester I, II dan III dengan Menggunakan Alat Photometer 5010 V5+ di Puskesmas Kasihan 1 Mega Shelvy Yana; Suryanto; Rosmita Anggraeni
Jaringan Laboratorium Medis Vol 8 No 2 (2026): November 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i2.15671

Abstract

Anemia during pregnancy is a significant health problem that may increase the risk of complications for both the mother and the fetus. This study aims to describe anemia among pregnant women in the first, second, and third trimesters based on hemoglobin measurements performed using the Photometer 5010 V5+ at Puskesmas (Community Health Center) Kasihan 1, Bantul Regency, in 2025. This observational study employed a cross-sectional design and used secondary data obtained from the laboratory test results and medical records of pregnant women from January to December 2025. The sample consisted of 159 pregnant women selected using purposive sampling. The data were analyzed descriptively using SPSS and presented as frequency distributions, percentages, means, standard deviations, and minimum and maximum values. The results showed that the mean hemoglobin concentration was 10.038 ± 0.6360 g/dL, with a minimum of 7.4 g/dL and a maximum of 10.9 g/dL. The mean hemoglobin concentrations in the first, second, and third trimesters were 10.217 g/dL, 9.745 g/dL, and 10.067 g/dL, respectively. Most participants (117 women) were classified as having pathological anemia, while 42 women (26.4%) were classified as having physiological anemia. Among those with pathological anemia, 109 women (93.2%) had mild anemia and eight women (6.8%) had moderate anemia.
Antibiotic Susceptibility Profile of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Diabetic Foot Wounds Yasmin Husnul Khotimah; Oos Fatimah Rosyati; Hafizah Ilmi Sufa; Ira Gustira Rahayu
Jaringan Laboratorium Medis Vol 8 No 2 (2026): November 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i2.15694

Abstract

Diabetic foot wound infection is a complication of diabetes mellitus that can be caused by various bacteria, including Methicillin-Resistant Staphylococcus aureus (MRSA), which may complicate the selection of antibiotic therapy because of its high level of resistance. This study aimed to identify MRSA isolates and determine their antibiotic susceptibility profiles from diabetic foot wounds of patients with diabetes mellitus at RUMAT Diabetes Wound Care, Bandung City. This descriptive laboratory experimental study used 30 diabetic foot wound samples collected from patients with diabetes mellitus. The isolates were identified by culture on Blood Plate Agar and Mannitol Salt Agar, followed by Gram staining, catalase test, coagulase test, and MRSA screening using cefoxitin discs. Antibiotic susceptibility testing was performed using the Kirby–Bauer disk diffusion method on Mueller–Hinton Agar according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The results showed that 7 (23.3%) isolates were identified as MRSA. All MRSA isolates were obtained from patients with type 2 diabetes mellitus who had been diagnosed with diabetes for at least one year. The MRSA isolates exhibited 100% susceptibility to azithromycin and amikacin; 85.7% susceptibility to tetracycline, gentamicin, ciprofloxacin, levofloxacin, and trimethoprim-sulfamethoxazole; 71.4% susceptibility to amoxicillin, erythromycin, and clindamycin; and 57.1% susceptibility to chloramphenicol. These antibiotic susceptibility profiles may provide useful information to support the selection of antibiotic therapy for diabetic foot wound infections in Bandung City.

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