Jaringan Laboratorium Medis
Jurnal Online Jaringan Laboratorium Medis adalah peer-reviewed journal yang mempublikasikan artikel-artikel ilmiah tentang ilmu kesehatan yang meliputi bidang Analis Kesehatan yang meliputi Penelitian Bakteriologi, Kimia Klinik, Parasitologi, Toksikologi Klinik, Hematologi, Sitohistoteknologi dan Imunologi, Teknologi Bank Darah. Dengan e-ISSN: 2685-8495 diterbitkan oleh Jurusan Analis Kesehatan Politeknik Kesehatan Kemenkes Semarang, terbit 6 bulan sekali, pada bulan Mei dan November setiap tahunnya.
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<![CDATA[Profil Mikroskopis Jaringan Ginjal Mencit (Mus Musculus) yang Difiksasi dengan Madu Konsentrasi 10% Selama 24 Jam ]]>
<![CDATA[Brilian, Tasya Vita]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 3 No. 2 (2021): November 2021 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v3i2.8066
<![CDATA[Fixation is used to maintain tissue structure in its original form “life-like state†and can protect proteins and tissue components from degeneration. The solution commonly used is 10% NBF. Formaldehyde is chemical substance that is toxic and not environmentally friendly, several studies have shown alternative substitutes fixation, one of which is the honey solution. The study of Mohammed et al (2020) fixated tissue with honey 10% and 20% shown good coloring properties and similar clarity to fixated with formalin 10%. Honey has acidic and dehydrating properties allow most microorganisms to be killed so that tissues will last for a long time. The research objective is to findout the description of microscopic of mice (Mus musculus) kidney tissue which were fixation using 10% honey solution for 24 hours. The research is included in qualitative descriptive research. The research design used was a non-eksperimental with a purposive sampling study approach. The sample used was 32 preparation with total of microscopic overview is 160. Microscopic image of mice (Mus musculus) kidney tissue fixed using 10% honey solution for 24 hours in 80 visual fields were 12.5% of the preparations is not good and 87.5% is good preparations. The microscopic image of mice (Mus musculus) kidney tissue fixed using 10% NBF is better than of the microscopic image of mice (Mus musculus) kidney tissue fixed with 10% honey for 24 hours.]]>
<![CDATA[Hubungan Antara Higiene Sanitasi Terhadap Kontaminasi Telur Cacing Non STH Pada Sayur Lalapan di Warung Makan ]]>
<![CDATA[Setyowatiningsih, Lilik]]>;
<![CDATA[Wikandari, Ririh Jatmi]]>;
<![CDATA[Surati, Surati]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 4 No. 1 (2022): May 2022 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v4i1.8325
<![CDATA[Fresh vegetables can be a source of transmission of worm infections to humans. The behavior of washing fresh vegetables that are not good by housewives and sellers of fresh vegetables in food stalls becomes large in transmitting worm infections if there are worm eggs that are still attached to fresh vegetables. The most common contamination found in fresh vegetables were eggs of the intestinal nematode Soil Transmitted Helminths (STH) as well as non-STH and trematodes. The purpose of this study was to determine the relationship between sanitation hygiene and contamination of non-STH worm eggs in fresh vegetables at food stalls in Semarang City. This type of research is an observational study with a cross sectional approach. Based on the results of laboratory tests, from a total of 165 samples examined, eggs of non-STH worms with species of Fasciola sp and Toxocara sp were found in 45 food stalls selling fresh vegetables. Based on bivariate analysis, there was no significant relationship between personal hygiene (p = 0.071) and environmental sanitation (p = 0.334) on contamination of non-STH worm eggs in fresh vegetables in food stalls in Pedurungan District, Semarang.]]>
<![CDATA[Kontaminasi Soil Transmitted Helminths (STH) pada Daun Bawang (Allium fistulosum L.) ]]>
<![CDATA[Cahyaningrum, Dwi Ratna]]>;
<![CDATA[Wikandari, Ririh Jatmi]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 4 No. 2 (2022): November 2022 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v4i2.8351
<![CDATA[Soil Transmitted Helminths (STH) infection is still a health problem in Indonesia. This infection is caused by intestinal nematodes which in its transmission require soil media. Vegetables can be a medium of transmission of STH. STH transmission occurs when the soil is contaminated with STH worm eggs and the worm eggs stick to vegetables. In addition, STH contamination can occur in traditional markets with poor sanitation such as humid rooms, floors with lots of puddles, muddy water, and improper placement of waste. The aim of the study was to describe the contamination of Soil Transmitted Helminths on Scallion (Allium fistulosum L.). This research is a descriptive research, the examination of worm eggs is done by indirect method with sedimentation technique. The research sample was taken from 25 traders. Leeks contaminated with STH as many as 15 (60%) samples from 25 traders. The type of STH found was hookworm rhabditiform larvae. There is STH contamination in leeks sold at the Bangetayu market, Semarang City. To avoid the risk of being infected with STH, people are expected to consume vegetables that have been cooked.]]>
<![CDATA[Kadar Kreatinin Serum pada Pasien Rawat Inap yang Didiagnosis Diabetes Melitus Tipe 2 ]]>
<![CDATA[Armaningrum, Tyas Pertiwi]]>;
<![CDATA[Budiharjo, Teguh]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 4 No. 1 (2022): May 2022 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v4i1.8352
<![CDATA[Uncontrolled Diabetes Mellitus will lead to long-term complications, namely decreased organ function, especially the kidneys, nerves, eyes, blood vessels and heart. Diabetic nephropathy is a complication that is often found in DM patients and attacks the kidneys. Serum creatinine examination is used to assess kidney function in patients with Type 2 Diabetes Mellitus. This purpose of research is to describe the description of serum creatinine levels in inpatients diagnosed with Type 2 Diabetes Mellitus at Dr. Kariadi Semarang. This study uses descriptive quantitative. The data comes from the medical records of inpatients diagnosed with Type 2 DM at Dr. RSUP. Kariadi Semarang who examined creatinine levels in the period January - April 2020 with the total sampling method. From this study, inpatients diagnosed with Type 2 DM at Dr. Kariadi Semarang were 79 respondents with male sex as many as 49 people (62%) and women 30 people (38%). In 79 respondesnts, there are 2 respondents (2.5%) had low creatinine levels, 7 respondents (8.9%) had normal creatinine levels, while 70 respondents (91.1%) had high creatinine levels. 43 male patients (88.6%) were included in the category of high creatinine levels. Based on age, it was dominated by the late elderly (55 – 65 years) as many as 32 people (40.5%) with the late elderly patients as many as 28 people (87.5%) having high creatinine levels. Conclusion: In 79 respondesnts (100%), there are 72 respondents (91.1%) who have abnormal creatinine levels and 7 respondents (8.9%) have normal creatinine levels.]]>
<![CDATA[Perbedaan Kadar Trigliserida Menggunakan Serum dan Plasma Edta dengan Biosystem A15 ]]>
<![CDATA[Aminah, Sarah]]>;
<![CDATA[Sari, Indah]]>;
<![CDATA[Bastian, Bastian]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 4 No. 1 (2022): May 2022 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v4i1.8402
<![CDATA[Trigycerides are the main lipid component in food intake, which is about 98% of total lipids and the remaining 2% consists of phospholipids and cholesterol. Triglycerides are present in adipose tissue, skeletal muscle, liver, lungs, and intestines to  provide energy in metabolic processes. Who estimates about 1 bilion individuals are overweight and about 300 milion individuals are defined as obese, normal triglyceride levels usually do not exceed 150 mg/dl, if triglyceride levels increase by more than 200 mg/dl then called hypertriglyceridemia. The research aims to determine the difference in triglceride levels using serum and plasma EDTA conducted at the Center For Health Laboratories (BBLK) Palembang. The type of research used is cross Sectional with research designintact group comparison. The sample consisted of 30 serums and 30 plasma EDTA taken from 30 respondents. Research is carried out starting from the preparation of patients, taking examination materials, processing of examination materials, analysis and results of research. The average levels of triglyceride examination using serum and plasma were obtained consecutively at 0.91 mmol/L and 0.83 mmol/L. Mann Whitney test results found that the significant value was p = 0.240. The p value obtained is p>0.05. The results can be concluded that there is no difference in examination of triglyceride levels using serum and plasma EDTA.]]>
<![CDATA[Tingkat Pengetahuan terhadap Donor Darah pada Masyarakat Pendonor ]]>
<![CDATA[Afrianti, Dina]]>;
<![CDATA[Susilawati, Elis]]>;
<![CDATA[Sofyanita, Eko Naning]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 4 No. 1 (2022): May 2022 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v4i1.8445
<![CDATA[Blood donation is an activity to donate blood for transfusion purposes. The number of voluntary blood donors in Indonesia is around 20% of the total production of blood bags per year, the rest is met from replacement donors. People are generally reluctant to donate blood because of fear of pain when blood is drawn using a syringe, fear of running out of blood, resulting in anemia, contracting the disease through blood donors, feeling unwell to donate blood and feeling that it is enough to only donate blood once. This is a factor that causes the limited number of donors due to limited knowledge. Research Objectives: To see an overview of the level of knowledge about blood donors in the community of donors at Pekalongan City. Methods: The type of research used is descriptive quantitative with a cross sectional approach with a questionnaire to determine the level of knowledge of the donor community. Results: Most of the blood donors at the Pekalongan City are Male 73 respondents (73%), the highest percentage aged 17-30 years as many as 54 respondents (54%), high school education, namely as many as 49 respondents (49%). Most of the donor knowledge level is said to be good, namely 88 respondents (88%). Conclusion: Most of the donor community at the Pekalongan City have a good level of knowledge about blood donation.]]>
<![CDATA[Profil Mikroskopis BTA Pada Sputum Keluarga Pasien Terdiagnosa BTA Positif Fase Lanjutan ]]>
<![CDATA[Masitah, Imaniah Nur]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 4 No. 1 (2022): May 2022 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v4i1.8469
<![CDATA[Tuberculosis (TBC) is an infectious disease caused by the bacterium Mycobacterium tuberculosis. The high prevalence of pulmonary tuberculosis in the community, the coverage of the prevention program with the DOTS strategy is still low, so the aspects of controlling transmission risk factors are important, especially transmission of high risk to the families of sufferers. The method of examination performed to diagnose TBCÂ is microscopic examination of sputum. This study aims to determine the microscopic description of Acid Resistant Basil (ARB) in family members who live in one house with patients with TBCÂ smear positive treatment advanced phase at the Balkesmas Semarang area. This research method is descriptive (non-experimental) with total sampling. The results of the study were that there was no ARBÂ in the sputum of family members living in one house with patients with TBCÂ smear positive treatment advanced phase of 31 samples from 17 respondents with 11 (32.3%) sputum samples mixed with saliva, 20 (58.8%) samples saliva and 3 respondents did not give two samples (8.8%). The conclusion of the study is the microscopic description of examination of sputum preparations as many as 31 samples from 17 respondents and 6 families 100% no BTA was found.]]>
<![CDATA[Profil Kadar Asam Urat Pada Buruh Konveksi Bagian Penjahitan ]]>
<![CDATA[Putri, Hawa Tirta]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 4 No. 1 (2022): May 2022 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v4i1.8470
<![CDATA[Hyperuricemia can cause arthritis gout. One of the factors causing hyperuricemia is the inactivity of a person due to work. For example, workers who sew in clothing manufacturers generally sit for a long time. Other factors that can increase uric acid levels are age, sex, hereditary history, and consumption of high purine foods. Purpose of the study was to determine uric acid levels in garment manufacturers in the sewing section in Grenggeng Village, Kebumen Regency. The method is this study was observational descriptive with cross sectional approach. Examination of uric acid levels was carried out using Point Of Caring Test method. The results is subjects of the study were 30 garment manufacturing workers in the sewing section. There were 16 subjects who had normal uric acid levels (53%) and 14 subjects had uric acid levels above normal (47%). Uric acid levels above normal in the age group 50-60 years were 33.3%, in the age 40-49 years 10%, in the age 30-39 years 3.3%. Subjects who sit for a  < 8 hours/day were 63,3%, while who sit for > 8 hours / day were 36,7%. Subjects who had above normal uric acid levels while sitting > 8 hour/day were 16.7%, while those sitting < 8 hours/day were 30%. Subjects with a history of hyperuricemia were 30%, and consumed high purine foods more than 5 times/week were 20%. Conclusion  is 53% of factory workers who make sewing clothes have normal uric acid levels and 47% above normal levels.]]>
<![CDATA[Profil Kadar Glukosa Darah Puasa dengan dan Tanpa Minum Air Putih ]]>
<![CDATA[Nurrois, Karenima Eka Wahyu]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 4 No. 2 (2022): November 2022 ]]>
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DOI: 10.31983/jlm.v4i2.8471
<![CDATA[Blood glucose levels are influenced by factors related to pastients and laboratory factors. There are various ways to treat blood glucose that have increased or decreased. Water consumption helps the process of removing all toxins in the body, including excess sugar. In James’s research (2010), that drinking water causes a breakdown of sugar. This research is to Know the description of fasting blood glucose levels with and without drinking water. Descriptive type research with cross sectional approach. Blood glucose levels of 28 student of TLM was examined by POCT methode and the respondent filled out the questionnare. The results of blood glucose levels are described based on questionnares that have been filled in the form of respondents diagram. From the results of examination 28 respondents, mean blood glucose level in conditions without drinking is 82,03 mg/dl, respondents with a minimum level of 65 mg/dl and a maximum of 97 mg/dl. Mean blood glucose level in conditions with drinking is 78,71 mg/dl, respondents with minimum level of 69 mg/dl and maximum of 104 mg/dl. Respondent’s fasting blood glucose levels in conditions without drinking and drinking decreased by 4%. There was a decrease in fasting blood glucose levels in respondents conditions without drinking and by drinking water, also influenced by physical activity factors, nutrition intake, drugs/vitamin and obesity.]]>
<![CDATA[Profil Kualitas Mikroskopis Sediaan Hepar Mencit (Mus musculus) dengan Pemotongan Ketebalan 2µm, 5µm dan 8µm ]]>
<![CDATA[Elen, Mircha Restiana]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 4 No. 2 (2022): November 2022 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v4i2.8483
<![CDATA[The cutting stage is the network manufacturing stage which needs to be considered the thickness scale. The standard scale that is commonly used is 2-7 µm, while the thickness of 5 µm which is used as a standard for cutting the thickness of the liver tissue shows that the cell is good for cutting liver tissue in mice (Mus musculus). Mice have many advantages, while the liver organ is one of the organs that are often used in research. The aim of this study was to describe the microscopic quality of liver preparations of mice (Mus musculus) by cutting tissue thickness of 2 µm, 5 µm and 8 µm. This type of research includes experimental research with descriptive research criteria. The study design used a purposive sampling study approach. The results of the quality of microscopic images of mice tissue (Mus musculus) at a thickness of 2 µm there were 12% not good, 20% not good and 68% good. The thickness of 5 µm is 100% good, while the thickness of 8 µm is 8%, the preparation is not good,  36% is not good, and 56% is good. The conclusions of this study were microscopic tissue mice (Mus musculus) preparations with good quality 68%, 8 µm 56%, and 100% at a thickness of 5 µm.]]>
