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The Intraperitoneal-Ketoprofen-Histopathological Induced Alterations in the Wistar Rat Kidneys
Samer Riyadh fadhill, Hakim Bahlok Jebur2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i2.3347
The current-focused study was carried out to generate a data profile about expected changes that could be induced in the kidneys of rats due to the use of intraperitoneal ketoprofen (KP). The study involved using 24 adult male Wistar rats sorted randomly into four groups (six animals per group). One group was treated as a control group, C, which was supplied with distilled water (DW) only. The remaining animals were presented as (50KP), (25KP), or (12.5KP) groups that received 50mg/kg.b.w., 25mg/kg.b.w., or 12.5mg/ kg.b.w., respectively, of KP. The experiment was continued for 70 days, and the kidney tissue samples were collected from the scarified animals at the end of day 70 of that experiment. The kidney tissues of the 50KP animals revealed dilation of the tubules throughout the outer strip of the outer medulla with necrosis and sloughing of the epithelial cells of the proximal convoluted tubules (PCTs). However, the 25KP group suffered lesser grades of epithelial-cell sloughing of the PCTs with lower levels of dilation of tubules than those recorded in the 50KP group. On the other hand, the kidney tissues of the 12.5KP group showed only dilation in the PCTs. The present experimental data unveil the side effects generated by the use of the intraperitoneal KP in the examined rat kidneys which should be used as a launching set of information for better use or further study this drug and its side effects in human patients.
Association between IL12A Gene of G/A genotype Polymorphism and Pulmonary Tuberculosis Risk in Baghdad Population
Anwar Abed Nasser Dhabaan1, Mayes Abdul-Hadi Mahdi2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i2.3348
The study included eighty samples. This cases-controlled study was performed including fifty Pulmonary tuberculosis patients, their ages ranged from 12 to 77 year (27 female and 23 male) and thirty controls (healthy), their ages ranged from 19 to 58 year (15 female and 15 male). We confined the frequency of IL12A gene (G/A rs568408 genotype) polymorphism by Tetra-ARMS PCR (Tetra amplification refractory mutation system-polymerase chain reaction) technology. Also, we determined the association of IL12A Gene (G/A rs568408 genotype) polymorphism with Pulmonary tuberculosis patients in Baghdad. Statistical results showed significant difference in genotype frequency of IL12A Gene (G/A rs568408 genotype) polymorphism between Pulmonary tuberculosis patients and control (healthy). The G allele shows high frequency in Pulmonary tuberculosis patients comparison with control (healthy) and present related with etiological fraction risk of Pulmonary tuberculosis patients and its ratio 62% in patients and 51.67% in control (healthy), While A allele shows high frequency in control (healthy) comparison with Pulmonary tuberculosis patients and present related with protective fraction of Pulmonary tuberculosis patients and its ratio 48.33% in control and 38% in Pulmonary tuberculosis patients. The genotypes of GG and AA homozygotes shows high frequency in Pulmonary tuberculosis patients comparison with control (healthy), and its ratio 42% and 18% respectively in Pulmonary tuberculosis patients, while its ratio 3.33% and 0% respectively in control (healthy), also GG and AA genotypes appear related with etiological fraction risk of Pulmonary tuberculosis patients, while the GA heterozygote show high frequency in control (healthy) and its ratio 96%, GA genotypes related with preventive fraction of Pulmonary tuberculosis patients. Our findings demonstrate that the IL12A Gene (G/A rs568408 genotype) polymorphism may represent a significant risk factor for pulmonary tuberculosis patients in Baghdad population.
The Effect of Allogenic Bone Marrow on Integration Dev-Mesenchymal Stem Cell And Vascular Endothelial Growth Factor
Ferdiansis
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i2.3349
Background: The integration of the tendon graft within the bone tunnel is one of the decisive success factors of the Anterior Cruciate Ligament (ACL) reconstruction. In ACL reconstruction that uses tendons as graft, the healing process that occurs in the bone tunnel is healing with fibrotic tissue. Aim: This study aims to determine the role of bone marrow mesenchymal stem cells and vascular endothelial growth factor intraarticularly in improving the biomechanical strength. Methods: This study uses experimental research design by using animal as a testing tool. The animals used in this study are male New Zealand white rabbits (Oryctolagus cuniculus), with the weight ranging of 2000 grams up to 3000 grams. Results: From the experiment, all samples from both treatment and control groups were all evaluated and no post-operative complication was found. At the evaluation time of 3 weeks, the difference between the mean of maximum strain rate between the treatment group and the control group was not statistically significant (p >0.05). At the evaluation time of 6 weeks, it was found that the difference in mean value of the maximum strain load force between the treatment groups versus the group was statistically significant. Conclusions: From this study, it can be concluded that intraarticular BM-MSC and VEGF administration can increase the ultimate tension strength of postoperative graft tendon rosary ACL reconstruction.
Influence of BRACA Usage on Self Improvement of Breast Cancer Patient by Post Mastectomy
Dewi Maryam1, Iskandar Ali1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i2.3350
Background: Breast cancer is an abnormal tissue, rapid growth and uncontrolled cells found in breast tissue. This body image disorder is thought to be related to the occurrence of post-mastectomy depression. Objectives: To know the influence between the use of BRACA with self-image improvement in patients with post-mastectomy breast cancer in Dr. Soetomo General Hospital, Surabaya. Method: The subject was a patient who has undergone a mastectomy on one side of the breast. The study design was a queasy experimental type of pre-post control design by sorting the sample and dividing it into 2 groups where 1 control group (not using BRACA) and another group in intervention using BRACA. Statistical analysis using t-test. Result: There was an increase in the self-image of respondents who intervened with BRACA’s use of pre and post use by showing a significant effect between BRACA use and self-improvement of post-mastectomy breast cancer subjects in Dr. Soetomo General Hospital, Surabaya (p = 0.000). Conclusion: The major cases occur on the right side of the breast with the duration of surgery more than one month. BRACA can be used as an alternative to improve the patient’s self image after mastectomy as it increases the self-image of the patients.
Correlation of Quadriceps and Hamstring Muscles Strength with Knee Osteoarthritis Stages and Pain Levels in Elderly Female Subjects
Mohammad Fathul Qorib 1, Hening Laswati1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i2.3351
Background: Osteoarthritis (OA) is one of the most common disease occur in elderly. It is commonly occurs in knee, hips and interphalangeal joints Pain caused by knee OA may result in functional disorders that affect patient’s life quality. Objective: To analyze correlation of quadriceps and hamstring muscles strength with knee OA stages and pain levels in elderly female subjects. Methods: 38 elderly female subjects (average age of 67.18 ± 4.91) were assessed for pain scale using numeric rating scale (NRS). We used 1RM protocol (EN-Tree) to measure quadriceps and hamstring muscles strength. We performed anterior-posterior and lateral knee X-rays to determine OA stage. The process of data analysis was conducted using several tests, including Kolmogorov-Smirnov test, Pearson’s correlation test, Spearman’s correlation test and Mann-Whitney test to measure difference between knee OA level using 1RM quadriceps muscle and quadriceps/hamstring (Q/H) ratio. Results: The average NRS was 3.68 ± 1.19, 1RM quadriceps and hamstring muscles strength were 2.68 ± 1.23 and 1.11 ± 0.44, respectively, quadricep/hamstring ratio (Q/H) was 2.53 ± 0.95. A total of 30 subjects had OA stage 2, while 8 subjects had OA stage 3. Conclusion: The 1RM quadriceps and hamstring muscles was not correlated with NRS. There was no difference in 1RM quadriceps and hamstring muscles between subjects with OA stage 2 and 3. We found a positive correlation between Q/H ratio and the NRS. The Q/H ratio in subjects with OA level 2and 3 was different.
Effect of Dates (Phoenix dactylifera L.) on Liver of Broiler Chicks Infected with Infectious Bursal Disease Virus. Biochemical and Histological Study
Balqees Hassan Ali1, Ziad Khalaf Jalob2, Zaid Yaseen Ibrahim3, Ahmed Sami Jarad4, Mustafa Salah Hasa
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i2.3353
A number of (80) birds (Ross 308) at one day old were obtained from a local hatchery in the area. The birds were allotted into four equal groups (no.=20) and were supplied with basal scientific diet. Palm date (PD) was supplied to G3 and the birds were challenged with virulent Infectious Bursal Disease Virus (IBDV). G4 was treated with palm date 3 days prior to challenge age. G2 was challenged without supplementation of PD. G1 was neither given PD nor challenged. Inclusion rate of PD was 10%. The challenged age was at 33 days old. Blood serum was assayed for evaluation of transaminases and lipid profile 10 days post infection period (PIP). The result showed that histopathological investigation is more accurate in determining the health state of the birds and the palm date have to be supplied in more than 10% to give its significant effect.
Identifying the Correlation between the Incidence of Breast Cancer and Hepatitis B Virus of Iraqi Women
Minen Al-Kafajy
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i2.3356
Objective: Women are half of the Iraqi community and their health should be priority to the country. Breast cancer Occurs is increasingly rising in Iraq due to so many problems that the country has suffered. Several privious studies have studied breast cancer; however, none of them has explore the presence of HBV and the basic molecular mechanism are generally obscure and research studies are not enough and disintegrated. Method: Therefore, this study are focusing on discovering a novel technique to detect breast cancer as early as possible to kept women lives. Advancement in routine breast cancer risk assessment will prevent chemotherapy uptake in women. subsequently , the women with or without breast tumor have expose to plasma assay to reveal HBV, Estrogen, Progesterone, complete blood count .The women test are divided into many classes of such as age , smoke and non-smoke to test their ability to detoxify carcinogenic aromatic amines in cigarette smoke. Result: 64% of the malignant women were smoker while 36% were none smoker. 45±12 years is the most vulnerable age to get cancer. HBsAg were demonstrated in 10% of the all malignant ladies, while antiHBs Ag have detected in only 5 % of the total number. ,complete blood counts and hormonal levels were dysregulated in the breast cancer patients. Conclusion: The hormones are negatively correlated with the presence of cancer, HBV have detected in several samples but the no significant correlation. Smoking is the most factor significantly associated with breast tumor . For all these reasons .Iraqi government strongly argue to make smoking cigarettes prohibited in public areas.
Cyclic Fatigue Resistance of Wave One Gold, F6 SkyTaper, One Curve, and AF Blue R3 NiTi Rotary Instrumentation Systems
Zainab M. Abdul- Ameer1, Samar Abdul Hamed2, Rasha H. Jehad3, Jacob Al-Hashemi4
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i2.3360
Objective: This in vitro study is aimed to compare and evaluate the cyclic fatigue of four varying NiTi rotary instrumentation systems. Method: In this study, four types of rotary files were used in four groups (10 files for each group), namely, Group A: Wave One Gold; Group B: AF Blue R3; Group C: One Curve; Group D: F6 SkyTaper. These groups were evaluated by a cyclic fatigue apparatus to measure cyclic fatigue resistance within the artificial metallic simulating canal that has a 60° angle of curvature, the curvature radius was 5 mm, whereas the inner diameter of the canal was 1.5 mm. All the files were rotated in artificial canals until they fracture. The resistance to cyclic fatigue was determined by counting the number of cycles to fracture, and the time to failure was recorded in seconds then transformed to minutes. In addition to that the fractured fragment length was evaluated. Results: One-way analysis of variance test showed a significant difference (P<0.001) in the average values of cycle number and time needed for file fracture and average fractured portion length among all groups. The t-test results indicate a significant difference (P<0.001) among all groups. Conclusions: The study concluded that with an apical curvature of an artificial canal with an angle curvature of 60°, AF Blue R3 and F6 SkyTaper instruments exhibit a higher resistance to cyclic fatigue than One Curve and Wave One Gold files most possibly due to surface and alloy feature variation.
Relation between Cardiorespiratory Fitness Measured with Six-Minute Walk Test and Walking Speed Measured with 1— Meter Walk Test in Patients of Post-Subacute and Chronic Ischemic Stroke
Hening Laswati1, Cut Jaswanita Eka Putri1, Patricia Maria Kurniawati1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i2.3362
Background: Cardiorespiratory fitness measurement using 6MWT (Six Minute Walking Test) and walking speed measurement using 10MWT (Ten Minute Walking Test) in post-stroke patients are measurement to assess post-ischemic stroke patients in subacute or chronic phase. This study is to assess cardiorespiratory fitness and to measure walking speed of post-stroke patients in subacute and chronic. We also aim to observe the relation between cardiorespiratory fitness and walking speed in post-stroke patients. Method: This study involved 38 subjects as samples. 6MWT assessment was conducted by measuring the mileage in six minutes. Walking speed was conducted afterwards with self-speed and fast-speed which were respectively assessed through three repetitions. Results: We found string correlation in 6MWT assessment results with mileage in meter and VO2 max (r = 0.680 and p = 0.000). The result of 6MWT measured with VO2 max (ml/kg/minute) indicated strong correlation with 10MWT both with self-speed (r = 0.715, p = 0.000) and fast-speed (r = 0.687 p = 0.000). Conclusions: We concluded that there was a relation between cardiorespiratory fitness and walking speed in post-subacute and chronic ischemic stroke patients.
Comparison Study of Urinary Retention Incidence in Assisted Vaginal Delivery Case with and without 24-Hour Catheterization
Eighty Mardiyan Kurniawati1, Hari Parathon2, Suskhan Djusad1, Fernandy Moegni1, Junizaf1, Budi Iman
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v14i2.3365
Background: Urinary retention is a condition commonly seen after vaginal delivery, especially in the highrisk cases, such as assisted vaginal delivery, grade 3-4th perineal rupture, or another high risk. Urinary retention caused by unsynchronized between the contraction of the bladder detrusor. Urinary catheterization is one of the preventions of urinary retention. It gives a time for perineal trauma to relieve and no longer edema, so that urethra can be fully relaxed. Therefore, 24-hour catheterization expected to prevent bladder overdistention. It also prevents the bladder from becoming atonia. Objective: This research aims to study the incidence of urinary retention in assisted vaginal delivery with and without 24-hour catheterization. Method: This study used randomized control trials that compared two groups with 24-hour catheterization and without 24-hour catheterization. This study was conducted on 40 women in each group who experienced assisted vaginal birth at Dr Soetomo Hospital, Indonesia. Result: Six women (15%) experienced urinary retention with 24-hour catheterization and six women (15%) had urinary retention without catheterization. There was no significant difference in the incidence of urinary retention with 24-hour catheterization and without 24-hour catheterization (p-value = 1.00). Also, there was no significant interference of urinary retention in the normal and prolonged second stage of labor (p-value = 0.736), and there was no significant risk factor contributing to urinary retention. Conclusion: No significant difference in urinary retention occurred in assisted vaginal delivery with and without 24-hour catheterization