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INDONESIA
Jurnal Ilmu Pertanian Indonesia
Published by Institut Pertanian Bogor
ISSN : 08534217     EISSN : 24433462     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Artikel yang dimuat meliputi hasil-hasil penelitian, analisis kebijakan, dan opini-opini yang berhubungan dengan pertanian dalam arti luas, seperti agronomi, ilmu tanah, hama dan penyakit tanamam, ilmu kehewanan, kedokteran veteriner, keteknikan pertanian, teknologi industri, teknologi pangan, ilmu gizi, keluarga dan konsumen, biometri, biologi, klimatologi, peternakan perikanan, kelautan, kehutanan, dan sosial-ekonomi pertanian yang telah dipertimbangkan dan disetujui oleh Dewan Editor. Keterangan mengenai peralatan, pengamatan, dan teknik percobaan akan diterima sebagai artikel CATATAN. Pedoman Penulisan dicantumkan pada setiap terbitan tercetak. Indeks Penulisan dan subjek serta daftar penelaan (mitra bestari) dicantumkan di tiap nomor terakhir pada setiap volume.
Arjuna Subject : -
Articles 975 Documents
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Transfer Gen Badh2 Termutasi Varietas Aromatik Mentik Wangi ke Varietas Nonaromatik Ciherang Djarot Sasongko Hami Seno; Bambang Padmadi; Dewi Praptiwi; . Sugihartati; . Taufiq; Muhammad Taufan Fatahajudin; Helmy Ramadhan Al Anshary; Tri Joko Santoso; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 1 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Replacement of non-fragrant-native badh2 gene with mutated badh2 of fragrant rice is an alternative to engineer new fragrant rice varieties with good agronomic traits as those of non-fragrant. Fragrant gene (mutated badh2) of Mentik wangi donor was introgressed into non-fragrant Ciherang host through site-directed crossing. Mentik Wangi was crossed with Ciherang, and the progeny was further backcross until BC3F1. Bradbury marka-assisted PCR was used to select progeny in every cross and backcross generation. Ciherang, Mentik Wangi, and their cross/backcross showed different PCR profiles. The statues of badh2 gene (native/mutated), as well as alleles (homozygote/heterozygote) between samples were identified. Mutated badh2-introgression was also observed within the selected heterozygote cross or backcross progenies (Fl, BCl, BC2, and BC3), indicated successful transfer of mutated badh2 gene from donor to host.
Bakteri Probiotik Dalam Budidaya Udang: Seleksi, Mekanisme Aksi, Karakterisasi, dan Aplikasinya Sebagai Agen Biokontrol Widanarni Widanarni; Sukenda Sukenda; Mia Setiawati
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 2 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Bacterial disease attack occurs at the hatchery stage, which is considered to be the most serious threat, and often results in mass mortality of shrimp larvae by vibrosis which is that caused by a luminous bacterium identified as Vibrio harveyi. This research was carried out to obtain local isolates of probiotic bacteria that were able to inhibit the growth of V. harveyi and effectively apply it as a biocontrol of vibriosis in shrimp cultures. The research was carried out as follows: (1) In vitro and in vivo selection of probiotic bacteria candidates, (2) Study of the action mechanism and characterization of the selected pro biotic bacteria, (3) Study on application of the selected probiotic bacteria as a biocontrol agent in shrimp cultures. Results of in vitro and in vivo selection provided the best three isolates, which were 1Ub, SKT-b and Ua. The survival rate of shrimp larvae which were not only inoculated by V. harveyi but also with 1Ub, SKT-b and Ua probiotic bacteria were 88.33, 83.33, and 81.67% respectively; where as the positive control treatment (merely inoculated with V. harveyi) gave a 41.67% survival rate and the negative control (without bacterial addition) was 68.33%. Studies using a rifampicin resistant marker (RfR) demonstrated that the number of V. harveyi MR5339 RfR cells in treatments without probiotic addition were higher than the treatment with the probiotic bacteria, in dead larvae, living larvae, as well as in the culture media. Partial sequencing of the I6S-rRNA gene showed that the I Ub isolate was similar to Pseudoalteromonas piscicida, whereas the SKT -b and Ua isolates were similar to Vibrio alginolyticus. Selected probiotic bacteria could be applied directly to shrimp larva culture media, or orally through enrichment of both natural and artificial food. Keywords: Penaeus monodon larvae, probiotic bacteria, vibriosis 
Hidrolisis Pati Garut Secara Enzimatis untuk Pembentukan Siklodekstrin Erliza Noor
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 2 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Modified starch has important role in chemical, cosmetic, pharmaceutical, and food industries. Cyclodextrin was prepared based on garut starch using starch hidroying enzime namely 0-amylase, 0-amylase, pullulanase and glukoamylase. Cyclication to form cyclodextrin was obtained using CGTase. The highest concentration of cyclodextrin was obtained by glucoamylase and CGTase concentration of 150 unit/g substrate which was 81.11g.l-1  in 90 minutes. Keywords: 0-amylase, 0-amylase, cyclodextrin, CGT-ase, gantt starch, glukoamylase, pullulanase 
Distribusi Kelas Diameter Pohon pada Berbagai Tipe Veget Asi di Gunung Salak, Bogor, Jawa Barat Muhammad Wiharto; Cecep Kusmana; Lilik Budi Prasetyo; Tukirin Partomihardjo
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 2 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The research objective was to study tree vegetation structure based on diameter class distribution at some vegetation types on Salak Mountain. Sample was taken at north, south, east, and west facing slope of Salai Mountain using line transect. Systematic sampling with random start was used to lay the transetcs. Measuring stem diameter at breast height was done in order to study the tree diameter class distribution. Non-parametric U Man Whitney statistic was used to know whether there was a different in number of individual at all diameter class in each vegetation type. At mix forest and plantation forest, the tree diameter class distribution forming J curve shape. At bamboo forest, the individual number increase at the highest class diameter. The number of individual trees ware highest at mix forest and lowest at bamboo forest.Keywords: diameter class, J reserve curve, mount salak, vegetation type
Kunci Identifikasi Ordo Thysanoptera pada Tanaman Pangan dan Hortikultura Dewi Sartiami
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 2 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Thysanoptera is a minute insect. It acts as pest, plant virus vector and predator. In this research, the thrips on crop and horticulture have been collected at Bogor and its surrounding. The result of this research was found 16 specieses which is consist of two suborders, that are Tubulifera and Terebrantia. On this two subordos, there are three families, Phalaeothripide, Aeolothripidae and Thripidae. This research also present an identification key of Thysanoptera which is built from these 16 specieses. The images of thrips characters were captured with digital camera to complete the explanation of identification key.Keywords: horticulture, identification, plant crop, thysanoptera
Perilaku Disolusi Ketoprofen dan Indometasin Farnesil Tersalut Gel Kitosan-GG Purwantiningsih Sugita; Achmad Sjahriza; Bambang Srijanto; Budi Arifin
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 2 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Chitosan, a modification of shrimp-shell waste, has been utilized as microcapsule. However, it's fragile gel property needs to be strengthened by adding glutaraldehyde (glu) and natural hydrocolloid guar gum (gg). This research's purposes were to determine rheological properties of chitosan-guar gum gel, to study diffusion and dissolution behaviour of ketoprofen and infar through optimum chitosan-guar gum gel membrane and microcapsule, respectively, and to test the coating stability of both medicines by the gel microcapsules, which are new drug's preparation, to determine their shelf lives and to predict the degradation mechanisms. This research was designed in six (6) steps: (1) chitin isolation and chitosan synthesis; (2) synthesis and optimization of chitosan-guar gum gel membrane; (3) in vitro study of ketoprofen and infar diffusion behaviour through the optimum membrane; (4) synthesis and optimization of chitosan-guar gum gel microcapsule to coat ketoprofen and infar; (5) in vitro study of ketoprofen and infar dissolution behaviour from the optimum microcapsule; and (6) physical and chemical microcapsule stability test using relative humidity (RH) and temperature controlled climatic chamber method. Studies on ketoprofen diffusion through chitosan-guar gum membrane showed that the formation of membrane small pores were appeared to be caused by membrane swelling, which was supported by the forcing force resulted from the difference of ketoprofen concentrations in the diffusion cells and from the temperature increase. This unique pore opening process is excellent for drug delivery process as a microcapsule. Spray drying process had successfully coated ketoprofen and infar in chitosan-guar gum microcapsule. Optimization by using Minitab Release 14 software showed that among the microcapsule compositions studied, [gg] and [glu] of 0.35% (w/v) and 3.75% (v/v), respectively were optimum to coat ketoprofen, whereas [gg] and [glu] of 0.05% (w/v) and 4.00% (v/v), respectively were optimum to coat infar, at constant chitosan concentration (1.75% [w/v]). In vitro dissolution profile showed that chitosan-guar gum gel microcapsule was more resistant in intestinal pH condition (rather basic) compared with that in gastric pH (very acidic). From stability test, formulation of ketoprofen preparation composed of 1.75% (w/v) chitosan, 0.35% (w/v) gg, and 3.50% (v/v) glu, was relatively the best, ·with ketoprofen percentage left in microcapsule after 3 months, degradation rate constant, and shelf life of of 80.33%, 0.0351 % week-1 and 18.92 months, respectively. The degradation of ketoprofen was seem to follow autocatalytic reaction mechanism controlled by the formation and growth of reaction core. In the other hand, the formulation with composition of 1.75% (w/v) chitosan, 0.19% (w/v) gg, and 5.00% (v/v) glu, was relatively the best microcapsule, with infar percentage left in microcapsule after 3 months, degradation rate constant, and shelf life of 77.67%, 0.0008 %-2 week-1 , and 4.28 week or about 30 days, respectively. The degradation of infar was presumably caused by hydrolysis.   Keywords: Chitosan-guar gum, diffusion, dissolution, stability
Protein Antimikrob Dari Tanaman Trichosanthes Sukma D; IM Artika; ET Tondok
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 2 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The research was aimed to study morphology, growth, development, pest and disease of 3 Trichosanthes species,  initiate shoots, callus and hairy root culture in vitro, analyze chitinase and peroxidase activities and the effect of salicylic acid (SA) and etefon (ETF) on the chitinase and peroxidase activities of crude protein extract from Trichosanthes, and evaluate in vitro antifungal activity of crude protein extract of Trichosanthes. The results of the research showed the differences of morphological characters, growth habit of T. cucumerina var. anguina, T.tricuspidata and the differences of pest and diseases problem of T. quinquangulata. T. cucumerina var. anguina and T. quinquangulata. T. tricuspidata had the highest chitinase activity in crude protein extract of in vitro shoots, calli and plant roots and peroxidase activity in plant roots grown in field. T. cucumerina var. anguina showed the highest chitinase and peroxidase activities in crude protein extract of plant roots grown in field and calli. Chitinase and peroxidase activities of calli crude protein extract of T. tricuspidata could be increased by SA and ETF. Adversely, ETF decreased the peroxidase activity of calli crude protein exract ofT. tricuspidata. In T. cucumerina var. anguina, SA could not increase the chitinase activity but increase the peroxidase activity. The crude protein from in vitro shoots of T. tricuspidata could inhibited the spore germination of Fusarium sp. from T. cucumerina var. anguina, Fusarium oxysporum from shallot, Puccinia arachidis from peanut and Pseudoperonospora cubensis from cucumber. The protein could not inhibit spore germination of Curvularia eragrostidis from Dendrobium orchids.   Keywords: antifungal, chitinase, etephon, peroxidase, salicylic acid, Triclwsanthes
Revitalisasi konservasi tumbuhan obat keluarga (toga) guna meningkatkan kesehatan dan ekonomi keluarga mandiri di desa Contoh Lingkar Kampus IPB Darmaga Bogor Agus Hikmat; Ervizal A.M. Zuhud; . Siswoyo; Edhi Sandra; Rita Kartika Sari
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 2 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Medicinal plants and traditional medicine for along ago are important role in the health care, stamina maintain, and treat diseases. Therefore medicinal plants and traditional medicines have strong root in the part of community up to now. Research on revitalization of family medicinal plant (TOGA) conservation done at Kampong Pabuaran (Cibanteng village), and Kampong Gunung leutik ( Benteng village), results indicated that research locations have completely medicinal plants diversity for medicine all diseases of village communities mentioned. Number of medicinal plants found at Gunung Leutik and Pabuaran Sawah Kampong (Cibanteng and Benteng Villages) were 237 spesies, and 95 spesies often used by respondents mentioned villages. Species number of medicinal plants have potential to expand based on use value of these species treat main diseases of community villages Gunung Leutik and Pabuaran Sawah Villages (Benteng and Cibanteng) were 15 spesies, such as: sambiloto (Andrographis paniculata (Burm.F) Ness.), meniran (Phyl/anthus niruri L.), takokak (Solanum torvum L.), pegagan (Centella asiatica (L.) Urban.), temulawak (Curcuma xanthorrhiza Roxb.), jahe (Zingiber officinale-purpurea Rose.), jeruk nipis (Citrus aurantifolia (Christm & Panz) Swingle), binahong (Anredera cordifolia), mahkota dewa (Phaleria macrocarpus (Sheff). Boerl.), rosella (Hibiscus sabdariffa), pule pandak (Rauvolfia serpentine (L.) Benth. ex. Kurz.), sangitan (Sambucus javanica Reinw.), sirih (Piper betle L.), brotowali ( Tinospora crispa), and kenikir (Cosmos caudatus).
Potensi Simpanan Karbon Pada Hutan Tanaman Mangium (Acacia mangium WILLD.) di KPH Cianjur Perum Perhutani Unit III Jawa Barat dan Banten Anindita Roesyane; Bambang Hero Saharjo
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 3 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

One of the best solution to reduce the increasing of carbiondioxide is keeping the forest and its ecosystems sustainably. It should be done because the forest could store the carbon stock at high level capacity. The research purposed to calculate the above ground carbon stock of planted A.mangium. Planted A.mangium in the year 2002 and 2004 used for calculation. Research done during April to May 2010 ath KPH Cianjur, State Own Company Perhutani Unit III, West Java and Banten. Data collecting through plot establishment in the field that was 20 m x 20 m for trees biomass and 2 m x 2 m sub-plot for litter and understorey. Results of research shown that the carbon stock of A.mangium planted in the year was 43.30 ton/ha while planted in the year 2004 was 27.70 ton/ha. 
Aplikasi Berbagai Marka Aromatik pada Varietas Padi Indonesia Djarot Sasongko Hami Seno; Satya Nugroho; Tri Joko Santoso; Dimas Adrianto; Dewi Praptiwi; Aniversari Apriana; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 3 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

This research applied various badh2.7 and badh2.2 fragrant markers (Bradbury et al., 2005b, Lang and Buu 2008, Shi et al., 2008, Sakthivel et al., 2009) on popular Indonesia non-fragrant (Ciherang, Fatmawati) and fragrant (Pandan wangi, Rojo Lele, Mentik Wangi, Gunung Perak, Pulu mandoti, Pare Kembang, Sintanur) rice varieties. For comparison, IR64, Nippon bare and Taipei 309 were included. Rice DNA samples were isolated from young  leaves, and PCR amplified using each of those fragrant markers. Results using all badh2.7 markers were consistently supported the existence of 2 group badh2.7 mutation pattern, while the use of badh2.2 marker indicated that there was no ex on 2 mutation. Badh2.7 sequence analysis of non-fragrant Ciherang, and aromatik member of group 1 (Pandan Wangi), as well as group 2 (Mentik Wangi) showed different mutation pattern. 

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