cover
Contact Name
Sutiman Bambang Sumitro
Contact Email
berkalahayati@yahoo.com
Phone
+62341570631
Journal Mail Official
wulidanisa@berkalahayati.org
Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia
Location
Unknown,
Unknown
INDONESIA
Berkala Penelitian Hayati
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Articles 507 Documents
Effect of aluminum chloride against changes of the histological structure of the cerebral cortex of mice (swiss webster) Yulia Irnidayanti; Risa Eno Aprilyanti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 26 No 2 (2021): June 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.26.2.20213

Abstract

The toxicity of metal to living organisms, including human beings, was discovered a long time ago. In the present time, aluminum exposure is widely used for wrapping food to keep it taste and smell. However it will affect to human health security, specially the brain. This study aimed to investigate the changes of histological Structure on the cerebral cortex caused by aluminum chloride. The mice were injected intraperitoneally with AlCl3 200 mg/kg body weight, once every 3 days, totalizing 10 administrations. Control only was administration distilled water. Histological observations were carried out using the paraffin method. The results of this study show that aluminum chloride cause disorganization of the laminated cerebral cortex, vacuolization, karyolysis, congestion, and hemorrhage. The mice of one and two-months of age group were more susceptible to aluminum chloride than the mice of the three-months of age group. This research can apply for an assessment of the exposure risk to heavy metal. Increasing Al contamination can occur naturally or by anthropogenic activities. The accumulation of the heavy metal in the organism can occur by biomagnification, by which heavy metal is passed from one trophic level to the next within a food web. Therefore communities that live in urban areas potentially exposed to metals aluminum and should be more concerned related to understanding health risks. Further studies are needed to examine the above matters. We recommend calculating the Provisional Tolerable Weekly Intake of aluminum and implication on local people
Begonia ’Crested‘: A new variety of Begonia from interspecific hybridization of Begonia sudjanae C.A Janson × Begonia puspitae Ardi Hartutiningsih-M Siregar; Sri Wahyuni; Mustaid Siregar; Sutomo Sutomo; I Nyoman Lugrayasa; I Made Ardaka
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 1 (2021): December 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.1.20215

Abstract

Improvement of leaf shape characters in Begonia can be done through hybridization between two different species of Begonia. This study aims to produce a new variety of Begonia to better improve the quantitative, qualitative characters and increase its genetic diversity. The study was conducted in the green house of Bogor Botanic Gardens. Two native species of Indonesia, Begonia sudjanae C.A Janson as the female parent and Begonia puspitae Ardi as the male parent. The mature F1 seeds were sown and selections were made among the plants produced. Observation results the characteristics of the new variety Begonia ‘Crested’ as follows: stem type rhizomatous, leaves simple, ovate, peltate with strongly undulate base. Leaves margin is crenate and apex is acuminate. Colour on the upper surface are yellow green group RHS Colour Chart: (YG 144 A) strong yellow green, colour of veins on upper side is (YG 144 D) light yellow green. Inflorescence peduncle with 20 cm long branched symmetrically. The name Begonia ‘Crested’ as the selected F1 plants are propagated in a vegetative method with leaves cutting, registered on the Center of Plant Variety Protection (PPVTPP) by No. 846/PVHP/2020. This new variety is beautiful and exotic leaves ornamental Begonia which will be developed as commercials ornamental plants.
Begonia muricata Blume and Begonia serratipetala Irmsch durability as indoor pot plant in Eka Karya Bali Botanic Garden Siti Fatimah Hanum; Ayyu Rahayu; I Dewa Putu Darma
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 26 No 2 (2021): June 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.26.2.20217

Abstract

Begonia is very popular as a foliage plant and some begonia species grow up in pots. Begonia serratipetala and Begonia muricata are Indonesian begonia species that are well suited as indoor potted plants because of their leaf color and shape. In addition, the Bali Botanical Garden's landscape nursery would like to use these begonias for decoration in all buildings. However, it still needs to be studied how long the shelf life of the two begonias is in indoor environments in the highlands without air conditioning (AC). The aim of this study was to investigate the indoor shelf life of B. serratipetala and B. muricata. This research method consists of two steps. The first step was the acclimation phase and the second step was the indoor placement phase. Each begonia was repeated three times. Parameters studied included plant growth (plant height, leaves, leaf width, and leaf length) and environmental factors (sunlight intensity, air temperature, and humidity). The data were analyzed descriptively. Correlation analysis was used to determine the relationship between growth parameters and environmental factors. The result showed that both begonias (B. muricata and B. serratipetala) have similar persistence to indoor begonia species within two weeks, the ideal time for plant replacement
Floral biology and breeding system of endangered species: Parmentiera cereifera Seem. (Bignoniaceae) Janis Damaiyani
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 26 No 2 (2021): June 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.26.2.20216

Abstract

Parmentiera cereifera Seem. is an endangered species in the family Bignoniaceae. Related to the conservation effort, information about the reproductive biology of this species is very limited. Based on that, the objectives of this study were to provide knowledge about the reproductive properties such as floral biology and breeding system of P. cereifera. The research was conducted at Purwodadi Botanic Garden, from October 2019 to February 2021. Several important aspects of reproductive biology, including flower biology, pollen viability and stigma receptivity, pollen morphology and breeding system were investigated. The viability was tested with TTC (2, 3, 5–triphenly tetrazolium chloride) and stigma reseptivity was tested with hydrogen peroxide. The breeding system was determined based on outcrossing index (OCI) using Cruden's method. The results showed that P. cereifera flower was monoecious, had morphological character that supported nocturnal pollination. Stigma and anthers spatially separated, but there was no temporal separation of stigma receptivity and anther dehiscence. Based on the flower biology, the pollen ornamentation (reticulate type), and the Out-Crossing Index (OCI=4), the breeding system was outcrossing but partially self-compatible, required pollinators (zoophily)
Effect of management systems, practices, flock size, and age group on the growth performance of chicks from local chickens’ farmers Kelvin Ngongolo; Andrew Chota
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 1 (2021): December 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.1.20211

Abstract

The improved growth performance of chicks determines the production efficiency of chicken to local chicken farmers. Little has been done to understand the contribution of management systems and practices to the growth performance of chicks from farmers who are involved in rearing local chickens in Dodoma. We focused on assessing how the growth performance of chicks (length and weight) from local chicken farmers is affected by management systems (Free range and semi-intensive systems), management practices such as water provision, supplement feeding, regular cleaning, flock size, and size of banda (hut) used to raise chickens. Data were collected for 8 rounds within 2 months (once per week) from 5 farmers in three wards: Nkuhungu (n=2), Mbwanga (n=2), and Mtube (n=1). In each round, data were collected from 10 chicks per farmer (n=400 chicks for all 8 rounds and 5 farmers). Kruskal Wallis was used to determine the variation in growth performance while generalized linear model was used to assess the effect of flock size, management system on the growth performance of chicks. Growth performance was significantly increased and affected by age (P<0.05). The management system significantly contributed to the growth performance of the chicks (P<0.001). In addition, the small size of the banda (hut) with large flock size reduced the growth performance of chicks due to increased competition in terms of space, supplements, water supplied, feed provided, and treatment (P<0.05). In raising local chicken, local farmers need to be concerned in applying management practices such as supplement feeding, treatment, flock size and size of huts.
Bacterial infections in COVID-19 patients and their possible treatments. A review A'liyatur Rosyidah; Mohamad Padri; Paulus Damar Bayu Murti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 1 (2021): December 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.1.20212

Abstract

The COVID-19 pandemic, which started in the beginning of 2020 was triggered by a new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, severely affected various sectors, especially health. The effect of COVID-19 on patients is exacerbated by bacterial co-infections and secondary bacterial infections. There are few studies on how bacterial co-infections and secondary bacterial infections worsen COVID-19 patients, including in Indonesia. Therefore, it is necessary to update and summarize the understanding of bacterial infections characteristics to help optimize the diagnosis, prevention, and treatment decisions. Antibiotics have been used in COVID-19 patients to treat bacterial infections to date, which could contribute to antimicrobial resistance in the future. The review's objective is to summarize bacterial infections in COVID-19 patients and several possible treatments, including antibiotics, phage therapy, probiotics/prebiotics, and nanomedicine for antimicrobial peptides (AMPs) delivery
The portrait of neem leaves-based high performance wound healing activity on zebrafish Nour Athiroh; Ari Hayati; Istirochah Pudjiwati; Ahmad Taufiq; Nurul Jadid Mubarakati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 1 (2021): December 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.1.20214

Abstract

This study was designed to evaluate the role of sliced and dropping models of neem leaves (Azadirachta indica) and environmental factors on zebrafish wound shrinkage. This study employed two treatment models: neem leaf slices and drops model. The treatment in the neem leaf slice model was the control group (fish was injured without neem leaf slices treatment, and G1-G3 of each fish was injured at 0.3 cm + 0.5, 1, and 2 g. Treatment in the neem leaf drop model was control group (fish was injured without neem leaves drops, and G1-G3 of each fish was injured at 0.3 cm + 1, 2, 4 drops. Findings suggest that there is a significant difference between the control group by treating G1, G2, and G3 on the shrinkage of zebrafish wound area both in the neem leaf slice and drop models, but G1 was not significantly different from G2 and G3, as well as G2 and G3 both in the neem leaf slice and drop model. Neem leaves contain nutrients that play a vital role in the formation of collagen and the formation of new capillaries to help speed up the wound healing process. The healing process involves the dynamic interaction of physiological factors.
Prediction of protein venom epitope (kistomin) from Calloselasma rhodostoma using immunoinformatics to design vaccine based on epitope Zyana Fithri Nur Faizah; Nia Kurniawan; Fatchiyah Fatchiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 1 (2021): December 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.1.20213

Abstract

Vaccines based on epitope are alternative treatments for snakebite aside from anti-venom immunoglobulin, which is specific and not cross-reaction. However, the potential kistomin epitope has not been known. This study identified the region of T cells epitope and evaluated their immunogenicity to induce an immune response by in-silico. Sequences of kistomin were collected from Swiss-Prot with ID P0CB14. The physico-chemical and conserved domain of kistomin were predicted by using ProtParam and the NCBI database. The T cell epitope was predicted by using the Artificial Neural Network (ANN) method on the IEDB website. Epitopes with MHC-IC50 values >250 nM were further analyzed for conservation and immunogenicity on the IEDB website as well. After that, the candidate 9-mer epitope was interacted by simulated docking with four Major Histocompatibility Complex (MHC) molecules (5ENW, 6VB0, 3PGD, 6DIG). The conserved 9-mer epitope candidates with high immunogenicity and having similarities with the 15-mer epitope candidates are 4-VLLVTICLA-12 and 27-NVNDYEVVY-35. The 4-VLLVTICLA-12 candidate epitope interacted at β-sheet structure of four MHC. In contrast, The 27-NVNDYEVVY-35 candidate epitope interacted at α-helix and β-sheet structures of HLA-B*15:02 MHC. This study suggested 27-NVNDYEVVY-35 is potentially used as vaccine from envenomation Calloselasma rhodhostoma. In future studies, other alelles can be used to predict epitope from metalloproteinase domain in kistomin.
Potency of endophytic fungi from Nauclea orientalis L. as antioxidant producer Elisa Nurnawati; Hary Widjajanti; Vivi Hendra Sutandar; Maulida Harwati; Eka Amelia; Siti Alharzsa; Nina Tanzerina
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 1 (2021): December 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869//bphjbr.27.1.20216

Abstract

Nauclea orientalis L. is widely distributed in Indonesia. Secondary metabolites such as flavonoids, polyphenols, and saponins are abundant in the bark of N. orientalis L. These compounds have the potential to act as antioxidants. Endophytic fungi, through genetic transfer and coevolution, can produce the same metabolites as their host plant. As a result, understanding the potential of endophytic fungi from N. orientalis L. to produce antioxidant compounds that can be developed is critical. According to the findings of this study, twelve isolates have the potential to produce secondary metabolites with antioxidant properties. Three isolates had high antioxidant activity: DB2 was identified as Aspergillus minisclerotigenes with an IC50 of 21 g/mL containing tannins, terpenes, and flavonoids, AB3 as Colletotrichum perseae with an IC50 of 31 g/mL containing tannins and terpenoids, and AB1 as Diaporthe tulliensis with an IC50 of 48 g/mL containing tannins. The secondary metabolite group has the potential to be developed into an antioxidant agent
Could scoring tailed and dumbbell-shaped nuclei increase the sensitivity of micronucleus analysis as a biomarker of radiation exposure? Dwi Ramadhani; Arum Wulansari; Viria Agesti Suvifan; Isnaini Farida; Wiwin Mailana; Hartini Ahadiyatur Ru’yi; Syarifatul Ulya; Sofiati Purnami; Mukh Syaifudin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 1 (2021): December 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.1.20218

Abstract

In the cytokinesis-block micronucleus (CBMN) assay, micronuclei (MNi), nucleoplasmic bridges (NPBs), and nuclear budding (NBUD) are the most commonly analysed morphological types of nuclear abnormalities. In contrast, tailed and dumbbell-shaped nucleus have historically received little attention in the CBMN assay. Interestingly, the incidence of tailed and dumbbell-shaped nuclei in lymphocytes is closely related with that of dicentric chromosomes or NPBs in the CBMN assay. To provide a better picture of the implications and significance of tailed and dumbbell-shaped nuclei as markers of radiation exposure, a literature review was performed in this study. Twenty articles were found in PubMed, PubMed Central, and manually searched. The articles were screened and those that met the inclusion criteria and did not meet the exclusion criteria were reviewed by all authors. At the end, nine articles were included. In conclusion, the assessment of in vivo tailed nuclei in blood smears and accounting for the occurrence of dumbbell-shaped nuclei in the CBMN assay can increase the sensitivity of the CBMN assay for biodosimetry involving a high dose exposure.

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