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Elvi Rusmiyanto PW
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Protobiont: Jurnal Elektronik Biologi
Published by Universitas Tanjungpura
ISSN : -     EISSN : 23387874     DOI : http://dx.doi.org/10.26418/protobiont.
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Jurnal Protobiont merupakan jurnal peer-reviewed dan open access journal yang mempublikasikan hasil-hasil penelitian biologi tropis yang berkaitan dengan bidang: 1. botani, 2. zoologi, 3. ekologi, 4. mikrobiologi, 5. genetika, 6. ilmu lingkungan, dan 7. biologi konservasi. Jurnal Protobiont terbit setahun 3 kali, yaotu bulan Maret, Juli, dan November. Jurnal Protobiont diterbitkan dan dikelola oleh Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 358 Documents
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Analisis Sebaran Bakteri Coliform Di Kanal A Kuala Dua Kabupaten Kubu Raya Ahmad Mulyadi, Syaeful Arifudin, Siti Khotimah,
Jurnal Protobiont Vol 3, No 2 (2014)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v3i2.5546

Abstract

Canal A Kuala Dua is one of the water source used in daily activities by the society lives along that river banks. The occurence of markets and inhabitans activities such as bathing and washing can affect the water quality of Canal A Kuala Dua due to the disposals entering the water body. One of the bioindicator used to detect water pollution is coliform bacteria. The purpose of this research was to find out the dispersion pattern of coliform bacteria when ebb and flow occurs in Canal A Kuala Dua. The Research was done in November 2011 through March 2012. Sampling was done at 9 stations along Canal A Kuala Duan when the ebb and flow occurred. The density of coliform bacteria was calculated by MPN method and Escherichia coli qualitative test was done by IMVIC test. Statistics sofwares such as Microsoft Excel, SPSS 15 (t test), and Arcgis 10.1 were used in data anylisis. Based on its use, Canal A Kuala Dua is categorized into class C. Average values of coliform bacteria densities in Canal A Kuala Dua are 74,249 x 103 MPN/100 mL when the uptide occurs and 59,259 x 103 MPN/100 mL when the downtide occurs.
Aktivitas Antibakteri Fraksi Diklorometan dan N-Heksana Paku Sisik Naga (Drymoglossum piloselloides (L) Presl.) Terhadap Bakteri Staphylococcus aureus dan Salmonella typhi Elvi Rusmiyanto Pancaning Wardoyo, Heriyati Siti Khotimah,
Jurnal Protobiont Vol 5, No 3 (2016)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v5i3.17094

Abstract

The potential use of dragon scales have been used by people in Kalimantan as anti-inflammatory drugs, oral thrush and toothache. Dragon scales (D. Piloselloides) is one of epiphytic plants in association with other plants. This study aims to determine the antibacterial activity of the dichloromethane and n-hexane fraction of dragon scales against S. aureus and S. typhi. The research was conducted from October to December 2015 in the Pharmaceutical Chemistry Laboratory Faculty of Medicine, Wood Technology Laboratory Faculty of Forestry, Chemistry Laboratory and Biology Laboratory Faculty of Mathematics and Natural Sciences, Tanjungpura University, Pontianak. Dichloromethane and n-hexane fraction were obtained from the fractionation of methanol extract. Furthermore, both fractions in TLC and chromatogram profiles were visualized using UV 254 and 336 nm. Antibacterial test of D. piloselloides fraction was done by paper disc diffusion method. The concentration used in this study was 0.25; 0.30 and 0.35 g/ml, and as a comparison, Ciprofloxasin 0.005 mg/ml used as a positive control. Antibacterial activity test of dichloromethane fraction of dragon scales has only the ability to inhibit S. aureus, whereas the n-hexane fraction has no antibacterial activity against S. aureus and S. typhi. TLC test results showed that the dichloromethane fraction contains flavonoids compounds, while the n-hexane fraction contains terpenoids compounds.
INVENTARISASI MAKROINVERTEBRATA BENTIK DI SUNGAI MENTUKA KABUPATEN SEKADAU KALIMANTAN BARAT Sangau, Paulus; Junardi, Junardi; Rousdy, Diah Wulandari
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36848

Abstract

The existence of Ephemeroptera, Plecoptera and Trichoptera (EPT) macroinvertebrates in water can be used as the indicators to identify the water quality. This study aims to determine the diversity of Macroinvertebrates and physical and chemical factors in water of the Mentuka River. Macroinvertebrate sampling was carried out in February 2018 using random with surber and dip net at three stations spread overthe upstream, middle and downstream of the Mentuka River. The measurements chemicals and physical factors such as water temperature, brightness, current velocity, depth, TDS, TSS, acidity (pH), dissolved oxygen (DO) and free carbon dioxide (CO2), which is carried out together with macroinvertebrates sampling. There are 378 macroinvertebrates individuals belonging to nine genera, namely Polypedilum, Hydropsyche, Anthopotamus, Baetis, Acroneuria, Libellula, Rhyacophila, Parathelphusa and Macrobrachium in Mentuka River. The Ephemeroptera, Baetis is the most abundance with 5.76 ind/m2. Based on the diversity index, the Mentuka was categorized as moderately polluted river.
Pemanfaatan Tumbuhan pada Upacara Adat Tumpang Negeri Suku Melayu di Keraton Ismahayana Landak Irwan Lovadi, Uswatun Hasanah, Riza Linda,
Jurnal Protobiont Vol 3, No 3 (2014)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v3i3.7355

Abstract

Traditional Ceremony Tumpang Negeri is a tribal ritual of Malayan people that utilizes plants to prevent misfortune so that people of Landak Regency might be averted from menaces and that Landak egency might be a prosfer, safe, and peacefull land. The aim of this research was to investigate the plant spesies and their utilization in Tumpang Negeri. This research was conducted from January to March 2014. The method of respondence selection that used in this research was snowball. The result of this research shows that there are 23 plant species which belong to 12 families, have been used in Tumpang Negeri Ceremony. Most of the plants used belong 3 families which are Poaceae (5 species), Arecaceae (4 species), and Zingiberaceae (4 species). The plants are used as materials for making traditional snacks, rafts, and tumpang for offerings. Part of the plants that mostly used are the fruits (45 %), leaves (32 %), roots (18 %), and stems (5 %). The plants were harvested mostly from the forest (35 %), the botanical gardens (31 %), the home garden (30 %), and the fields. Every plant species used in Traditional ceremony tumpang Negeri has specifik meanings.
Profil GC-MS dan Potensi Bioherbisida Ekstrak Metanol Daun Ketapang (Terminalia catappa L.) terhadap Gulma Maman Ungu (Cleome rutidosperma D.C.) Mukarlina, Alia A. Gani, Elvi Rusmiyanto P. Wardoyo,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.19554

Abstract

Ketapang (Terminalia catappa L.) is one of the plants that contains allelochemical compounds which can suppress the growth of surrounding plants that can be utilized as bioherbicide. This research aims to determine the bioactive compounds that have the potential of bioherbicide and the effective concentration of the methanol extract of T. catappa leaf that can inhibit seed germination and growth of purple Cleome (Cleome rutidosperma). The results showed that treatment of the methanol extract of T. catappa leaf can inhibit seed germination and growth of weed C. rutidosperma. The GC-MS spectral data showed that bioactive compounds that have the potential bioherbicide of the methanol extract of T. catappa leaf are Neophytadiene, 3, 7, 11, 15-tetramethyl-2 -hexadecen- 1 ol, 2, 6, 10, 14, 18, 22- tetracosahexaene and Lupeol. The methanol extract of Ketapang (T. catappa) leaf included pre-grown bioherbicide with a concentration of 0.1 g/ml. The research was conducted in Macrobiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences. Evaporation was carried out in the Laboratory of Wood Technology, Faculty of Forestry and the soil chemical content analysis was conducted in Soil Chemistry Laboratory, Faculty of Agriculture, University of Tanjungpura, Pontianak. The Method of Gas chromatography–mass spectrometry (GC-MS) was conducted at the Organic Laboratory of Chemistry Department, Faculty of Mathematics and Natural Sciences, University of Gadjah Mada from August to November 2016. The research used a Completely Randomized Design (CRD) with extract concentrations of 0; 0.1; 0.3; 0.5 and 0.7 g/ml each with three replications.
PENGARUH KOTORAN AYAM DAN MIKORIZA Glomus aggregatum TERHADAP PERTUMBUHAN KEDELAI (Glycine max) PADA TANAH GAMBUT Iqbal, Muhamad; Linda, Riza; Mukarlina, Mukarlina
Jurnal Protobiont Vol 9, No 1 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i1.40621

Abstract

Peat soil in West Kalimantan has been contained low of nutrient level so it affects the growth of soybeans. One effort to increase the availability of nutrients in peat soils is by using mycorrhiza and chicken manure fertilizer. This research aims to determine the growth of soybeans by administering chicken manure and mycorrhiza on peat soils. The research was conducted from January to April 2019. The research design used was a Completely Randomized Design Factorial consisting of 2 factors with 4 replications. The first factor is mycorrhiza fungi: M1 (0 gr/polybag), M2 (5 gr/polybag), M3 (10 gr/polybag), M4 (15 gr/polybag) and the second factor is chicken manure fertilizer: P1 (0 tons/ha) , P2 (5 tons/ha), P3 (10 tons/ha), P4 (15 tons/ha). The results showed that the single chicken manure application significantly affected all observed parameters with 15 tons/ha chicken manure fertilizer. Mycorrhiza fungi just a significantly effect on fresh weight and dry weight. Also, the combination of mycorrhiza fungi and chicken manure fertilizer just a significantly affected wet weight and dry weight with combination 5 g mycorrhiza and 5 tons/ha of chicken manure fertilizer.
Karakterisasi dan Kepadatan Bakteri Nitrifikasi pada Tingkat Kematangan Tanah Gambut yang Berbeda Di Kawasan Hutan Lindung Gunung Ambawang Kabupaten Kubu Raya Riza Linda, Agustina Kiding, Siti Khotimah,
Jurnal Protobiont Vol 4, No 1 (2015)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v4i1.8708

Abstract

Nitrifying bacteria has an important role in oxidizing ammoniac to be nitrit which is required by plants. The purpose of this study is to investigate character and density of nitrifying bacteria at maturity level of peat soil. The sampel was taken at wildlife sanctuary of Ambawang mountain Kubu Raya district. The proses of isolation was conducted by pour plate method that applying spesific media of nitrification and total plate account was used to calculate the density of bacteria. Observation and identification was implemented macroscopically, microscopically and biochemically. The finding indicated that 5 genus of nitrifying bacteria had different level of maturity in peat soil. Nitrosomonas, Nitrobacter and Nitrosococcus were some types ofbacteria that were pound in fibrik meanwhile in hemik, Nitrobacter and Nitrosocystis were identified. In addition, in saprik, Nitrobacter, Nitrospina and Nitrosococcus were discovered. The total density of nitrifying bacteria colonies in maturity level of peat soil in fibrik, hemik and saprik was 160 x 105 CFU/g, 107,5 x 105 CFU/g, and 19 x 105 CFU/g.
Pertumbuhan dan Kandungan Pigmen Tanaman Keladi (Caladium bicolor Aiton Vent) pada Tanah yang Merkuri (HgCl2) Riza Linda, Apriana Anania, Mukarlina,
Jurnal Protobiont Vol 6, No 3 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i3.22479

Abstract

Mercury is one of the heavy metals and is toxic which causes a decrease in pH and soil fertility. Keladi (Caladium bicolor) is able to grow and adapt to heavy metal contaminated soil because it has a tolerance mechanism and contains antioxidant pigments. This research aims to determine the effect of mercury on the growth and the content of C. bicolor pigment. This research was conducted from January 2017 until March 2017 at Greenhouse and Biology Laboratory of the Faculty of Mathematics and Natural Sciences of Tanjugpura University Pontianak. The research was a completely randomized design with 5 replications consisting of control, concentrations HgCl2 300, 350, 400 and 450 ppm. The results showed that the concentrations of HgCl2 at 350, 400 and 450 ppm decreased the chlorophyll pigment content, wet weight and dry weight of C. bicolor. The concentrations of HgCl2 at 300 ppm increases the anthocyanin pigment content and HgCl2 had no significant effect on carotenoid pigment content.
Kadar Malondialdehyde Induk dan Struktur Morfologis Fetus Mencit (Mus musculus) yang Diperdengarkan Murottal dan Musik Rock pada Periode Gestasi Tri Rima Setyawati, Silvie Kurniasari, Ari Hepi Yanti,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.20819

Abstract

Gestation is a stressful state that involved physiological change. Environmental stressor could alter the adaptive mechanism during gestation, thus bring bad impact to the developing fetus. Music is commonly used as a therapy media to minimize stress during gestation. This research aims to examine the effect of listening murottal and rock music during gestation towards levels of oxidative stress in mouse dams (Mus musculus) and the morphological structure of the fetuses. The levels of oxidative stress were analyzed by measuring malondialdehyde (MDA) level as the biomarker. This research was conducted from July to November 2016 in Zoology Laboratory and Chemical Laboratory of Faculty of Mathematics and Natural Sciences Tanjungpura University Pontianak. Fifteen mice were divided into 2 experimental groups (murottal/M and rock/R) and 1 control group (K). During day 0 to day 15 of gestation, the pregnant mouse dams in group M and R were exposed to murottal and rock music, respectively. Laparatomy was conducted on the 16th day of gestation, followed by serum MDA measurements and observing the fetuses. According to the result, MDA level in group M was significantly lower (0.42±0.21 nmol/mL; p<0.05) than two other groups. This result accompanied by the normal fetuses with highest mean of weight (0.74±0.05 g) and length (18.72±0.90 mm) compared to fetuses from group R and K. Fetus with hemorrhage and intrauterine growth restriction (IUGR) were found in group R, while intrauterine mortality was found in group R and K.
AKTIVITAS ANTIFUNGI EKSTRAK METANOL DAUN AKASIA (Acacia mangium Willd.) TERHADAP Phytophthora sp. (Im5) SECARA IN VITRO Wulandari, Yanti; Rahmawati, Rahmawati; Mukarlina, Mukarlina
Jurnal Protobiont Vol 9, No 3 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i3.46101

Abstract

Phytophthora sp. are pathogenic fungi that can cause plant diseases with symptomatic rotten or known as stem base rotten disease (BPB). Acacia leaf (Acacia mangium Willd.) has secondary metabolite content so that it can be used as a source of natural antifungal substitutes for synthetic antifungal This study uses a completely randomized design (CRD) with 4 treatment levels consisting of control, extract concentration of 2 %, 3 %, and 4 %. Antifungal activity test uses food poisoning method by adding acacia leaf extract to PDA media. The results showed that the extract of acacia leaves (Acacia mangium Willd.) had an effect on inhibiting the growth of isolate Phytophthora sp. (Im5) with the decrease of fungi colony diameter. Concentration of 4 % extract is the best concentration with the very strong level of antifungal activity in inhibiting the growth of Phytophthora sp. (Im5).

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