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Life Science
Published by Universitas Negeri Semarang
ISSN : 22526277     EISSN : 25285009     DOI : https://doi.org/10.15294/lifesci
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Life Science publishes original and significant articles on all aspects of Life Sciences (Biology, Genetics, Biological Anthropology, Botany, Medical Sciences, Veterinary Sciences, Biochemical Genetics, Biometry, Clinical Genetics, Cytogenetics, Genetic Epidemiology, Genetic Testing, Evolution and Population Genetics, Immunogenetics and Molecular Genetics). The journal also covers ethical issues. It aims to serve as a forum for life scientists and health professionals.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 10 Documents
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Search results for , issue "Vol 3 No 2 (2014)" : 10 Documents clear
UJI TOLERANSI TANAMAN TEMBAKAU (Nicotiana tabacum L.)TERHADAP CEKAMAN KADMIUM (Cd), TIMBAL (Pb), DAN TEMBAGA (Cu) PADA KULTUR CAIR Rosidah, Siti; Anggraito, Yustinus Ulung; Pukan, Krispinus Kedati
Life Science Vol 3 No 2 (2014)
Publisher : Universitas Negeri Semarang

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Penelitian ini menyelidiki respon fisiologis, anatomis, dan morfologis tanaman tembakau (Nicotiana tabacum L.) terhadap cekaman logam berat: tembaga (Cu), kadmium (Cd), dan timbal (Pb). Uji dilakukan pada bulan Januari-April 2014. Sampel yang digunakan adalah tembakau umur 3-4 minggu yang dikecambahkan secara in vitro dan kemudian dipapar logam berat selama 14 hari. Desain penelitian yang digunakan yaitu rancangan acak lengkap dengan satu faktor: yaitu konsentrasi logam Cu (0 µM, 50 µM, 100 µM, 150 µM & 200 µM), Cd (0 µM, 50 µM, 100 µM, 200 µM & 300 µM), dan Pb (0 µM, 5 µM, 20 µM, 50 µM & 100 µM). Parameter yang digunakan antara lain: pertambahan panjang akar, pertambahan jumlah akar, akumulasi logam dalam akar, lokalisasi penimbunan dalam akar, dan warna daun. Bertambahnya konsentrasi logam menghambat pertumbuhan akar dan menyebabkan deposit logam pada jaringan akar dan gejala klorosis. Hasil uji Atomic Absorbtion Spectrosphotometry (AAS) menunjukkan semakin besar konsentrasi semakin banyak akumulasi logam pada jaringan akar. Akumulasi Cd pada konsentrasi 200 µM lebih besar dibanding pada konsentrasi 300 µM. Hal ini menunjukkan ada faktor lain selain konsentrasi seperti respon internal individu dan gangguan permeabilitas. Analisis kualitatif membuktikan bahwa cekaman Cu tidak berpengaruh signifikan terhadap warna daun, sedangkan pada cekaman Cd (100, 150 dan 200 µM) dan Pb (150 µM) daun mengalami klorosis. Secara umum pengaruh konsentrasi logam berat yang rendah seperti 50 µM Cu, 50 µM Cd, dan 5 µM Pb terhadap parameter fisiologis, anatomis, dan morfologis tidak berbeda nyata pada taraf 5 % dengan kontrol. Dengan demikian dapat disimpulkan bahwa tembakau mampu mentoleransi cekaman logam pada konsentrasi yang rendah.This research investigated physiological, anatomical, and morphological responses of tobacco (Nicotiana tabacum L.) stressed by heavy metals: copper (Cu), cadmium (Cd), and lead (Pb).The experiment was conducted from January-April 2014. The samples used are three-four weeks tobaccos germinated by in vitro then were exposed to heavy metals for 14 days. This study used completed random design with single factor: concentrations of Cu (0 µM, 50 µM, 100 µM, 150 µM & 200 µM), Cd (0 µM, 50 µM, 100 µM, 200 µM & 300 µM), and Pb (0 µM, 5 µM, 20 µM, 50 µM & 100 µM. Further, stress response is analyzed based on several parameters includes root elongation, root number, metal accumulation and localization in root, and leaf color. The increased metals had caused growth inhibition, metal deposit in root tissue, and chlorosis symptoms. Atomic Absorption Spectrosphotometry (AAS) results showed that higher concentration will cause root tissue accumulate more metals. Yet 200 µM of Cd accumulated more than 300 µM Cd, so it could be suggested that there are other factors than concentration in determining metal absorbtion such as internal individual respon and membrane permeability disturbance. In other hand,qualitative analysis proved that chlorosis was not found in Cu treatment but consistently notified in high concentrations of Cd (100 µM up to 200 µM) and Pb (100 µM). However, the influence of lower metals concentration such as 50 µM Cu, 50 µM Cd and 5 µM Pband control treatment were not significantly different at the level of 5% in physiological, anatomical, and morphological responses. Therefore, it can be concluded that tobacco was capable to tolerate low concentration of metal stress.
EFEKTIFITAS SALEP EKSTRAK BATANG PATAH TULANG (Euphorbia tirucalli) PADA PENYEMBUHAN LUKA SAYAT TIKUS PUTIH (Rattus novergicus) Qomariah, Siti; Lisdiana, Lisdiana; Christijanti, Wulan
Life Science Vol 3 No 2 (2014)
Publisher : Universitas Negeri Semarang

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Senyawa aktif yang terkandung dalam batang patah tulang yaitu glikosida, sapogenin dan asam elagat. Senyawa yang membantu mempercepat penyembuhan luka sayat yaitu senyawa sapogenin, karena senyawa sapogenin bermanfaat mempengaruhi pembentukan kolagen (tahap awal perbaikan jaringan). Tujuan penelitian adalah mengkaji ekstrak batang patah tulang dalam bentuk salep pada penyembuhan luka sayat tikus putih serta menentukan dosis dan waktu tercepat pada penyembuhan luka sayat. Penelitian menggunakan design post test yaitu dengan mengambil data setelah perlakuan, rancangan yang digunakan rancangan acak lengkap (RAL). Sampel yang digunakan yaitu 20 ekor tikus putih jantan galur Wistar umur 2 bulan yang dibagi menjadi 4 kelompok yaitu dengan poviodin iodine 10% sebagai kontrol positif, salep dengan dosis 5%, 10% dan 20%. Perlakuan diberikan selama 13 hari. Pengambilan data dengan mendokumentasikan dan mencatat waktu penyembuhan luka sayat. Data dianalisis secara deskriptif dengan membandingkan waktu tercepat pada penyembuhan luka sayat. Hasil penelitian menunjukkan bahwa salep dosis 5% dan 10% hasilnya tidak ada perbedaan. perlakuan salep dosis 10% lebih optimal dalam mempercepat penyembuhan luka sayat tikus putih dilihat pada hari ke-9 luka sudah sembuh dengan adanya jaringan baru. Sedangkan pada dosis 20% kurang optimal dalam mempercepat penyembuhan luka sayat dilihat pada hari ke-13. Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian salep dosis 10% dosis paling optimal dalam mempercepat penyembuhan luka sayat tikus putih dengan melihat waktu penyembuhan tercepat pada tikus putih.Active compounds contained in the stem fractures are glycosides , sapogenin and elagat acid . Compounds that help speed healing cuts that sapogenin compounds , as useful sapogenin compounds affect the formation of collagen ( the early stages of tissue repair ) . The purpose of the study is to assess the fracture stem extract in the form of ointment in healing cuts mice and to determine the dose and the fastest time in the healing of cuts . Research using post-test design is to take data after treatment , the design used a completely randomized design ( CRD ) . The samples used were 20 white male rats of the Wistar strain aged 2 months were divided into 4 groups, with 10 % poviodin iodine as a positive control , an ointment at a dose of 5 % , 10 % and 20 % . Treatment was given for 13 days . Data retrieval with document and record cuts healing time . Data were analyzed descriptively by comparing the fastest time in the healing of cuts . The results showed that the dose ointment 5 % and 10 % the result is no difference . Treatment ointment 10 % more optimal dose in accelerating the healing of cuts seen white mice on day 9, the wound had healed with the new tissue. While at 20 % less than optimal dose in accelerating healing cuts seen at day 13 . Based on the results of this study concluded that the administration of a dose of 10 % ointment most optimal dose in accelerating the healing of cuts of white mice by looking at the fastest healing time in rats .
EFEK PAPARAN KRONIK TIMBAL (Pb) PER ORAL PADA STRUKTUR HISTOPATOLOGIK LAMBUNG TIKUS PUTIH Aziz, Rahmat Al; Marianti, Aditya
Life Science Vol 3 No 2 (2014)
Publisher : Universitas Negeri Semarang

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Penelitian ini bertujuan menganalisis pengaruh toksikan Pb pada struktur histopatologik lambung tikus putih jantan galur Wistar yang dipapar Pb per oral. Sampel yang digunakan 10 ekor tikus jantan, dibagi menjadi dua kelompok. Kelompok I sebagai kontrol, kelompok II diberi perlakuan Pb asetat 175 mg/tikus selama 60 hari. Pada hari ke-61, tikus dikorbankan, organ lambung diambil untuk selanjutnya dibuat preparat histopatologik. Perubahan histopatologik yang diamati berupa adanya sel abnormal. Data sel abnormal selanjutnya dianalisis menggunakan Paired Sample t-test. Analisis data menggunakan Paired Sample t-test diperoleh hasil nilai sig. 0,038.< 5%, hal ini membuktikan bahwa rata-rata skor sel abnormal kelompok kontrol berbeda signifikan dengan rata-rata skor sel abnormal kelompok Pb asetat. Berdasarkan penelitian ini dapat disimpulkan bahwa paparan kronik Pb per oral menyebabkan perubahan pada struktur histopatologik lambung tikus putih. Karena paparan kronik Pb per oral dapat menyebabkan perubahan pada struktur histopatologik lambung, diharapkan bisa menjadi peringatan dini tentang bahaya Pb pada organ pencernaan.This study aimed to analyze the effect of the Pb toxicant on gastric histopathological structure male albino rats Wistar strain were exposed of Pb per oral. The sample used 10 male rats were divided into two groups. First group as control, and second group was given treatment of Pb 175 mg/rat for 60 days. On 61th day, the rats were sacrificed, the gastric organs were taken for histopathologic preparations. The observed histopathological changes were of abnormal cells. The abnormal cells data were analyzed with Paired Sample t-test. The data were analyzed using Paired Sample t-test, obtained sig. value 0.038. <5%, it  proved that the average of abnormal cells score of the control group was differ from the average of the abnormal cells score of Pb acetate group. Based on this study, can be concluded that Pb chronic exposure per oral could caused changes on the gastric histopathologic structure of albino rats. Because Pb chronic exposure per oral could cause changes on the gastric histopathologic structure, is expected to be an early warning about the dangers of Pb in the digestive organs.
JENIS CACING PADA FESES SAPI DI TPA JATIBARANG DAN KTT SIDOMULYO DESA NONGKOSAWIT SEMARANG Nezar, Muhammad Rofiq; Susanti, R; Setiati, Ning
Life Science Vol 3 No 2 (2014)
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Penelitian ini bertujuan mengetahui jenis, tingkat infeksi, dan metode yang paling efektif untuk identifikasi cacing pada feses sapi dengan pemeliharaan berbeda di TPA Jatibarang dan KTT Sidomulyo Nongkosawit Semarang. Jumlah sampel feses sapi sebanyak 64 sampel. Penelitian dilaksanakan pada bulan April-Mei 2014. Metode identifikasi menggunakan metode natif, sedimentasi, dan flotasi. Hasil identifikasi menunjukkan bahwa jenis telur cacing pada feses sapi di TPA Jatibarang sebanyak tiga belas spesies (Ascaris lumbricoides, Bunostomum phlebotomum, Haemonchus contortus, Oesophagostomum radiatum, Ostertagia ostertagi, Trichuris globulosa, Fasciola hepatica, Fasciola gigantica, Moniezia expansa, Moniezia benedeni, Paramphistomum cervi, Cotylophoron cotylophorum, dan Schistosoma bovis). Telur cacing pada feses sapi di KTT Sidomulyo sebanyak empat spesies (B. phlebotomum, H. contortus, O. ostertagi, P. cervi). Larva cacing hanya pada feses sapi KTT Sidomulyo sebanyak dua spesies (larva Trichostrongylus axei dan Strongyloides papillosus). Intensitas telur cacing pada feses sapi di TPA Jatibarang lebih tinggi daripada feses sapi dari KTT Sidomulyo yaitu H. contortus sebanyak 1080 epg dan pada feses sapi di KTT Sidomulyo intensitas tertinggi adalah O. ostertagi sebanyak 1000 epg. Berdasarkan metode identifikasi, metode natif efektif untuk identifikasi nematoda, Moniezia sp, dan trematoda. Metode sedimentasi efektif untuk identifikasi trematoda dan Moniezia sp, sedangkan metode flotasi efektif untuk identifikasi telur dan larva nematoda. Research has determined the species, infection level, and the most effective identification method of helminths in cattle faeces with different husbandry in Jatibarang landfill and KTT Sidomulyo Nongkosawit Semarang. Faecal samples were 64 samples. It was conducted in April-May 2014. The identification used native, sedimentation, and flotation methods. Results showed the species of helminths eggs of cattle faeces in Jatibarang landfill were thirteen species (Ascaris lumbricoides, Bunostomum phlebotomum, Haemonchus contortus, Oesophagostomum radiatum, Ostertagia ostertagi, Trichuris globulosa, Fasciola hepatica, Fasciola gigantica, Moniezia expansa, Moniezia benedeni, Paramphistomum cervi, Cotylophoron cotylophorum, and Schistosoma bovis). Helminths eggs found in KTT Sidomulyo Nongkosawit were four species (B. phlebotomum, H. contortus, O. ostertagi, and P. cervi). Larvae found in cattle faeces of KTT Sidomulyo were two species (larvae T. Axei and S. papillosus). Intensity of helminths eggs in Jatibarang landfill more than KTT Sidomulyo was H. contortus as much as 1080 epg and KTT Sidomulyo has the highest intensity of O. ostertagi as much as 1000 epg. Identification showed an effective method for nematodes, Moniezia sp, and trematodes were native. Sedimentation was the effective method for trematodes and Moniezia sp. identification, while flotation method was effective for eggs and larvae of nematodes.
STUDI AKTIVITAS ANTIBAKTERI SARI DAUN BINAHONG (Anredera cordifolia) TERHADAP PERTUMBUHAN Bacillus cereus dan Salmonella enteritidis Rahmawati, Fahmi; Bintari, Siti Harnina
Life Science Vol 3 No 2 (2014)
Publisher : Universitas Negeri Semarang

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Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri sari daun binahong (Anredera cordifolia) terhadap pertumbuhan Bacillus cereus dan Salmonella enteritidis. Sampel daun binahong diperoleh dari Desa Gentan Kecamatan Bendosari Kabupaten Sukoharjo. Konsentrasi sari daun binahong yang digunakan adalah 25 %, 50 %, 75 %, dan 100 %, dengan antibiotik kotrimoksazol sebagai kontrol positif. Uji aktivitas antibakteri sari daun binahong terhadap bakteri Bacillus cereus dan Salmonella enteritidis dilakukan secara in vitro menggunakan metode difusi kertas cakram melalui pengukuran diameter zona hambat. Data dianalisis one way Anova dengan tingkat kepercayaan 95% dan diuji lanjut LSD. Hasil penelitian menunjukkan bahwa sari daun binahong pada konsentrasi 100% dapat menghambat pertumbuhan Bacillus cereus dan Salmonella enteritidis masing-masing sebesar 9,64 mm dan 6,86 mm. Konsentrasi hambat minimum yaitu pada konsentrasi 25% dengan zona hambat 2,54 mm pada B. cereus dan 2,52 mm pada Salmonella enteritidis. Simpulan dari penelitian ini adalah daun binahong memiliki aktivitas antibakteri terhadap pertumbuhan Bacillus cereus dan Salmonella enteritidis, dan aktivitas antibakteri lebih besar ditunjukkan pada bakteri Gram positif yaitu Bacillus cereus. This study aims to determine the antibacterial activity of the binahong leaf extract on the growth of Bacillus cereus and Salmonella enteritidis. The sample is derived from plants grown in Gentan Bendosari Sukoharjo. The concentrations of the binahong leaf extract used are 25 %, 50 %, 75%, and 100%, with the antibiotic cotrimoxazole as a positive control. The test of binahong leaf extract antibacterial activity against bacterial of Bacillus cereus and Salmonella enteritidis is done in vitro using paper disc diffusion method by measuring the diameter of inhibition zone. The data is analyzed using one way Anova with 95% trust level and LSD test further. The results showed that the binahong leaf extract at a concentration of 100 % can inhibit 9,64 mm of Bacillus cereus growth and 6,86 mm of Salmonella enteritidis growth. The minimum inhibition concentration is at a concentration of 25 % with the inhibition zone 2,54 mm for Bacillus cereus and 2,52 mm for Salmonella enteritidis. The conclusions of this study is binahong leaves have antibacterial activity against the growth of Bacillus cereus and Salmonella enteritidis, greater antibacterial activity shown in Gram positive bacteria that Bacillus cereus.
Analisis Morfometrik dan Meristik Hasil Persilangan Ikan Pelangi Boesemani (Melanotaenia boesemani) dan Ikan Pelangi Merah Abnormal (Glossolepis incisus) Afini, Irsyah; Elfidasari, Dewi; Kadarini, Tutik; Musthofa, Siti Zuhriyah
Life Science Vol 3 No 2 (2014)
Publisher : Universitas Negeri Semarang

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Ikan pelangi merupakan jenis ikan hias air tawar yang diminati masyarakat, diantaranya adalah ikan pelangi boesemani dan ikan pelangi merah. Kedua jenis ikan tersebut merupakan ikan endemik yang berasal dari Irian Jaya dan termasuk kelompok ikan yang terancam punah. Tingginya minat masyarakat terhadap ikan pelangi menyebabkan breeder melakukan usaha budidaya dengan cara persilangan. Ikan hasil persilangan memiliki karakter fenotip yang khas meliputi, warna, bentuk, morfometrik dan meristik. Penelitian ini bertujuan untuk menganalisis variasi morfologi, karakter morfometrik dan meristik serta pola pertumbuhan individu ikan hasil persilangan antara ikan pelangi boesemani normal (jantan) dan ikan pelangi merah abnormal (betina). Pengukuran 30 karakter morfometrik dan meristik dilakukan terhadap ikan dewasa (hidup) yang berusia 9 bulan. Analisis data menggunakan analisis komponen utama (PCA), analisis perbandingan karakter meristik, analisis hubungan panjang-berat. Hasil PCA menunjukkan perbedaan karakter dan ciri khas morfometrik tertentu antara ikan hasil persilangan yang normal dan abnormal. Hasil analisis perbandingan karakter meristik menunjukkan bahwa kisaran nilai setiap karakter meristik tidak berbeda jauh dengan hasil penelitian sebelumnya. Hasil analisis hubungan panjang-berat menunjukkan bahwa ikan jantan normal bersifat allometrik positif, sedangkan ikan jantan abnormal dan ikan betina (normal-abnormal) bersifat allometrik negatif. Perbedaan dalam setiap parameter ini disebabkan oleh perbedaan bentuk dari tubuh ikan akibat keabnormalan. Rainbow fish is species of freshwater fish that interest the public, such as the boesemani rainbow fish and red rainbow fish. Both of these fishes are endemic fish from Irian Jaya and includes a group of endangered fish. The high of public interest cause a rainbow fish breeders to do cultivation by crossing way. The fish from crosses have distinctive phenotypic characters include, color, shape, morphometric and meristic. This study aims to analyze morphological variation, morphometric and meristic characters and growth patterns of individual fish from crosses between normal boesemani rainbow fish (males) and abnormal red rainbow fish (females). 30 characters of morphometric and meristic measurement were conducted on adult fish (live) 9 month old. Analysis of data by using principal component analysis (PCA), comparative analysis of meristic characters, analysis of the length-weight relationship. PCA results showed the characteristic differences in morphometric characters and certain fish from crosses between normal and abnormal. The results of comparative analysis of meristic characters show that the range of values of each character was not much different from the previous results of the study. The results of the analysis of the length-weight relationship showed that the normal male fish are positively allometric whereas, abnormal male fish and female fish (normal-abnormal) are negatively allometric. Differences in each parameter was caused by differences in the shape of a fish's body as a result of abnormality.
EKTOPARASIT PADA ORDO ARTIODACTYLA DI TAMAN MARGASATWA SEMARANG Dwiyani, Nurbaety Puspitasari; Setiati, Ning; Widyaningrum, Priyantini
Life Science Vol 3 No 2 (2014)
Publisher : Universitas Negeri Semarang

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Penelitian ini bertujuan untuk mengetahui jenis ektoparasit yang terdapat pada Ordo Artiodactyla di Taman Margasatwa Semarang. Jenis hewan dari Ordo Artiodactyla yang digunakan ada 5 jenis (Sapi Bali, Nilgae, Kijang, Rusa Tutul dan Rusa Timor). Penelitian ini dilaksanakan pada tanggal 23 September-23 Oktober 2013. Ektoparasit yang ditemukan dari hasil penyapuan dengan sikat dan sweepnet di kandang dan tubuh mamalia Ordo Artiodactyla Taman Margasatwa Semarang terdiri dari dari 2 ordo yaitu Ordo Diptera meliputi spesies Stomoxys sp, Chrysomya megacephala, Haematobia sp, Tabanus sp, Musca domestica dan Ordo Anoplura meliputi spesies Linognathus sp. Pada Sapi Bali di Taman Margasatwa Semarang pada tubuhnya ditemukan luka-luka serta keropeng yang sudah kering dan terdapat luka terbuka berdarah di pundak, bahu, leher, dan punggung. Hal ini dapat mengganggu ketenangan sapi karena iritasi yang ditimbulkannya dan luka tersebut merangsang datangnya lalat seperti Stomoxys sp dan Haematobia sp. Keterdapatan kutu Linognathus sp pada mamalia Ordo Artiodactyla di Taman Margasatwa Semarang relatif sedikit sehingga peranannya sebagai ektoparasit masih belum terlihat besar dampaknya. The aim of this research was identifying the ectoparasites found on the Order of Artiodactyla in Semarang Zoo. There are five types of animals used in this reasearch (Balinese cow, Nilgae, Antelope, Spotted Deer and Elk East ). This study was conducted on 23 September – 23 October 2013. Ectoparasites were acquired by brushing and sweeping using sweepnet tools in the cage and on the body of mammals of the order of Artiodactyla in Semarang Zoo. The ectoparasites obtained are 6 types, consisting of 2 orders, 4 families and 6 species. The results of the study showed that 6 types of ectoparasites are found in two order; the order of diptera that includes species of Stomoxys calcitrans, Chrysomya megacephala, Haematobia irritans, Tabanus sp, Musca domestica and the order of anoplura includes species Linognathus sp. In the case of Balinese Cow in Semarang Zoo, there were dried wounds and scaps found on the body and several opened bleeding sores were frequently discovered on the shoulder, neck and back. Those wounds may disturb the calm of cows because they cause irritation and the bleeding wounds have attracted flies such as Stomoxys calcitrans and Haematobia irritans. The existence of Linognathus sp ticks in the Order of Artiodactyla mammals in Semarang Zoo is relatively small so that its role as ectoparasites is not considered to cause significant impact.
DISTRIBUSI DAN KELIMPAHAN POPULASI Aedes spp. DI KELURAHAN SUKOREJO GUNUNGPATI SEMARANG BERDASARKAN PELETAKAN OVITRAP Fatmawati, Titi; Ngabekti, Sri; Priyono, Bambang
Life Science Vol 3 No 2 (2014)
Publisher : Universitas Negeri Semarang

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Nyamuk merupakan serangga yang memiliki peranan penting bagi kehidupan manusia. Aedes spp merupakan salah satu spesies nyamuk yang berperan sebagai pembawa vektor penyebab Demam Berdarah Dengue (DBD). Sukorejo salah satu Kelurahan dari 5 kelurahan yang bernaung di bawah Puskesmas Sekaran yang merupakan daerah dengan kasus DBD tertinggi. Penelitian ini bertujuan untuk mengetahui distribusi dan kelimpahan larva nyamuk Aedes spp. di Kelurahan Sukorejo Gunungpati Semarang berdasarkan peletakan ovitrap. Penelitian ini merupakan penelitian eksploratif. Pengambilan data dilakukan dengan cara mengamati ovitrap, mengidentifikasi larva, dan menghitung indeks ovitrap. Pemasangan ovitrap diletakkan di dalam dan di luar pemukiman, kebun dan sekolah dasar di 6 RW dan 3 SD. Hasil penelitian menunjukkan jumlah ovitrap positif terbanyak di RW 11 dengan Indeks Ovitrap 15,2% dan kerapatan 608 butir/ovitrap, dan terendah SD 2 dengan Indeks Ovitrap 0,9% dan kerapatan 71 butir/ovitrap. Hasil identifikasi menunjukkan spesies Aedes aegypti dan Aedes albopictus pada semua lokasi penelitian. Nyamuk Aedes spp. terdapat di semua lokasi penelitian dengan pusat distribusi di RW 11 dan memiliki skor 2 yang menunjukkan potensi kerawanan penyakit DBD rendah. Kelimpahan populasi tertinggi dilihat dari kerapatan jumlah telur per ovitrap terdapat di RW 11 Mosquito are insect that have an important role in human life. Aedes spp. is one of the species of mosquitoes which act as carriers of the vector causing Dengue Haemoragig Fever (DHF). Sukorejo is one of villages with the highest cases of DHF and it is also one of 5 villages which are supervised by Puskesmas Sekaran. The purpose of this research was determining the distribution and larvae abundance of Aedes spp. in Sukorejo Village, Gunungpati district, Semarang regency based on the location of ovitrap. The research was an explorative research. The data was taken by observing ovitrap, identifying larvae and calculating the ovitrap index. The ovitraps were placed inside and outside the houses, gardens on 6 RW (Neighborhood union) and 3 Elementary Schools. The results showed that the highest number of positive ovitrap was found in RW 11 with 15.2 % IO and with density of 608 grains/ovitrap, and the lowest number was found in Elementary School 2 of Sukorejo with 0.9 % IO and density of 71 grains/ovitrap. The final result of species identification of Aedes aegypti and Aedes albopictus is positively found in all locations. While Aedes spp. were distributed in all location with its distribution centers located in RW 11 and its score was 2 that showed the low risk potention level of DHF. The highest abundant that based on the density number of egg in each ovitrap was found in RW 11.
KEANEKARAGAMAN JENIS EKTOPARASIT BURUNG PARUH BENGKOK FAMILI PSITTACIDAE DI TAMAN MARGASATWA SEMARANG Bahtiar, Denny Herbianto; Susanti, R.; Rahayuningsih, Margareta
Life Science Vol 3 No 2 (2014)
Publisher : Universitas Negeri Semarang

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Abstract

Penelitian bertujuan mengeksplorasi keanekaragaman jenis ektoparasit pada burung paruh bengkok Famili Psittacidae di Taman Margasatwa Semarang. Populasi dan sampel penelitian adalah ektoparasit yang ditemukan pada 3 jenis burung paruh bengkok. Penelitian dilakukan pada tanggal 23 September sampai 23 Oktober 2013. Metode yang digunakan adalah deskriptif kualitatif menggunakan light trap dan penyisiran bagian tubuh burung meliputi kepala, tubuh, sayap dan ekor. Hasil pengamatan didapatkan 7 jenis ektoparasit, terdiri atas ordo Diptera meliputi Pseudolynchia canariensis, Chrysomya megacephala, Aedes sp, Culex sp, Anopheles sp dan ordo Mallophaga meliputi Menopon sp, Gonicotes sp. Bagian ekor paling banyak ditemukan ektoparasit yaitu Menopon sp, Gonicotes sp dan Pseudolynchia Canariensis, sedangkan pada bagian kepala tidak ditemukan ektoparasit. Jenis burung Cacatua sulphurea yang banyak terserang ektoparasit, hal ini dikarenakan kebersihan dan pemeliharaan kandang yang kurang diperhatikan. Gangguan ektoparasit pada burung paruh bengkok menyebabkan bulu rontok dan iritasi pada kulit. Hal ini dapat membuat burung gelisah, lebih diam dan nafsu makan berkurang karena iritasi pada kulit. Lingkungan kandang burung yang dekat dengan kebun menyebabkan ektoparasit yang tertangkap Light trap adalah dari jenis nyamuk (Aedes sp, Culex sp dan Anopheles sp). The aim of this research was exploring the diversity of ectoparasites found on parrot bird from the Family of Psittacidae in Semarang Zoo. This study was conducted on 23 September 2013 – 23 October 2013. There were methods used deskriptif kualitatif in this study was wholebody screening include head, body, wings, tail and light trap. Based on the observation there were obtained 7 types of ectoparasites that consist Diptera ordo includes Pseudolynchia canariensis, Chrysomya megacephala, Aedes sp, Culex sp, Anopheles sp and Mallophaga ordo includes Menopon sp, Gonicotes sp. The tail was founded ectoparatises is Menopon sp, Gonicotes sp and Pseudolynchia Canariensis, no ectoparasite were found on head. The samples from C. sulphurea have been attacked by ectoparasite because the cleanness and cage maintenance was less concerned. These ectoparasites infection had caused leather fall and skin irritation. While the ectoparasites found on the cage which located around garden are caught by light trap and most of them are mosquitoes; Aedes sp, Culex sp dan Anopheles sp. Mosquitoes bites are the vectors of several diseases and it may disturb the bird in the night.
EFEKTIVITAS SUBSTITUSI SITOKININ DENGAN AIR KELAPA PADA MULTIPLIKASI TUNAS KRISAN SECARA IN VITRO Indriani, Betty Shinta; R, Enni Suwarsi; Pukan, Krispinus K.
Life Science Vol 3 No 2 (2014)
Publisher : Universitas Negeri Semarang

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Abstract

Krisan (Chrysanthemum indicum L) merupakan salah satu komoditas bunga potong yang sangat digemari di Indonesia. Keindahan warna dan variasi bentuk bunga serta tingkat kelayuan yang rendah menyebabkan krisan banyak diminati. Minimnya pengadaan dan kualitas bibit merupakan salah satu masalah yang ada di lapangan. Perlu dilakukan penelitian untuk meningkatkan jumlah dan kualitas bibit krisan agar dapat memenuhi kebutuhan masyarakat. Penelitian ini termasuk penelitian eksperimental. Sampel yang digunakan berupa meristem lateral, menggunakan rancangan acak lengkap (RAK) dengan dua faktorial. Faktor pertama konsentrasi air kelapa (5%,10%,15%,20% & 25%), sedangkan faktor kedua adalah konsentrasi benzil adenin (BA) (0 ppm, 0.5 ppm, 1 ppm & 1.5 ppm). Multiplikasi krisan diukur berdasarkan tiga parameter pengamatan: tinggi eksplan, jumlah tunas & daun. Penelitian ini menggunakan analisis data ANAVA dua jalan dengan uji lanjut beda nyata terkecil (BNT). BA berpengaruh signifikan terhadap jumlah tunas dan jumlah daun namun tidak signifikan terhadap tinggi tunas, air kelapa berpengaruh signifikan terhadap semua parameter pengamatan. Multiplikasi tunas yang optimal dianjurkan untuk menggunakan interaksi BA 0.5 ppm dengan 10% air kelapa Chrysanthemum (Chrysanthemum indicum L ) was one of the most popular cut flower commodity in Indonesia. The popularity of this flower was caused by the beauty of various petals color and flower shape as well as high level of flower freshness. The lack of high quality seed provision was one of the problems which are currently existed. Based on that problem, it was urgent to conduct a research in order to improve the quantity and quality of chrysanthemum seedlings as well as fulfilling the market demand. This research was categorized as experimental research. The sample used in this study was lateral meristem of Chrysanthemum and the design used was a complete randomized design (RBD) with two factorials. The first factor was the concentration of coconut water (coconut endosperm) which was; 5%, 10 % , 15 % , 20 % & 25 %. While the second factor wass the concentration of benzyl adenine ( BA ) which was; 0 ppm, 0.5 ppm 1 ppm and 1.5 ppm ). Chrysanthemum multiplication was measured using three observations parameters; the height of explants, the number of shoots and leaves. Data were analyzed by using two- way ANOVA and using least significant difference (BNT) as the further test. The results of study that BA had significant effect on the number of shoots and leaves but has no significant effect on shoot height, while coconut water had significant effect on all observation parameters. In order to accelerate the multiplication of shoots and leaves of chrysanthemum, it was recommended to use the combination of BA 0.5 ppm with 10 % coconut water.

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