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Media Veteriner
Published by Institut Pertanian Bogor
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Veterinary
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Articles 3 Documents
 1 
Search results for , issue "Vol. 5 No. 1 (1998): Media Veteriner" : 3 Documents clear
The IPB-I Reagent As An Alternative Tool To Detect Subclinical Mastitis Mirnawati Sudarwanto
Media Veteriner Vol. 5 No. 1 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

Mastitis subklinis hanya dapat dideteksi melalui pemeriksaan mikrobiologi dan penghitungan jumlah sel radang terhadap contoh susu. Penyakit ini sangat merugikan peternak karena produksi susu menurun dan seringkali berkembang menjadi mastitis klinik atau kronis yang berakibat penyingkiran sapi lebih awal. Melakukan deteksi dini dengan pereaksi terhadapcontoh susu dapat memperkecil resiko tersebut. Ujimastitis subklinis di lapang yang ada sampai saat ini,seperti Calijbrnia Mastitis Test (CMT), masih jarangdilakukan karena harga pereaksinya cukup lnahal dansulit diperoleh di pasaran. Untuk memperoleh suatuteknik yang cepat dan pereaksi untuk uji mastitis subklinis di lapang yang relatif lebih mudah, murah dan bahan-bahannya mudah diperoleh di pasaran, maka dikembangkanlah pereaksi yang diberi nama "IPB- I ". Dari penelitian ini diperoleh data bahwa sensitivitas IPB- I, CMT, Whiteside Test (WST), Aulendorfer Mastitis Probe (AMP) mod- 1 dan AMP mod-2 berturut-turut 0,99; 0,92; 0,94; 0,92 dan 0,94. Sedangkanspesifisitasnya berturut-turut 0,92; 0,37; 0,32; 0,47 dan0,89. Nilai prediksi (predictive valz~e)I PB- I, CMT, WST, AMP mod-1 dan AMP mod-2 berturut-turut 0,95; 0,99; 0,97; 0,99 dan 0,97. Nilai Keterhandalan IPB-I lebih tinggi dibandingkan dengan pereaksi lainnya. Namun nilai prediksi untuk pereaksi masih harus diperbaiki.
The Inhibitory Effect of Aflatoxin on The Growht of Bacillus megaterium and Staphylococcus epidermidis Eko Sugeng Pribadi; Unang Patriana; Titiek Sunartatie
Media Veteriner Vol. 5 No. 1 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

B. megaterium and S. epidermidis were used to study the inhibitory effect or pure aflatoxin and aflatoxin-contaminated rice. Results showed that the inhibitory effect was more pronounced In B. megatarium growth. The inhibitory effect of pure aflatoxin started at the concentration of 20 μg/ml and persisted to the concentration of 25 μg/ml (P
The Influence of Homologous and Heterologous Sera in Culture Medium on in Vitro Maturation and Fertilization of Sheep Oocytes Ita Djuwita; Yohan Rusyiyantono; Kusdiantoro Muhamad; Bambang Purwantara; Yuhara Sukra
Media Veteriner Vol. 5 No. 1 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

The influence of various types of sera i.e. Fetal Calf Serum (FCS), Bovine Post Estrous Serum (SPES) as heterolog sera; and Ewes Fetal Serum (EFS) and Ewes Serum (ES) as homologue sera on in vitro maturation and fertilization of sheep oocytes was investigated using Tissue Culture Medium 199TM (TCM 199. Gibco). Sovine sera were collected from cow at seven days post-estrus, while ewes sera were collected during estrous (ES-HO) and six days post estrous (ES-H6). The result shows that the maturation rate of oocytes cultured in medium supplemented with EFS, ES-HO and ES-H6 at  concentrations of 32.9%, 68.7%, and 67.6% respectively is better than those supplemented with FCS and SPES at concentrations of 22.2% and 28.9%, respectively. In vitro fertilization rate were significantly higher in medium supplemented with ES-HO and ES-H6 at concentration of 65.4% and 65.8% (P < 0.05) than that supplemented with SPES at concentration of 31.7%. In conclusion, ewes sera could be used in culture medium for promoting both in vitro maturation and fertilization rate of sheep oocytes.

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