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Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa
Published by Universitas PGRI Adi Buana Surabaya
ISSN : 14121840     EISSN : 26219093     DOI : https://doi.org/10.36456/stigma.15.01
Core Subject : Education,
Mathematics
Jurnal STIGMA adalah jurnal ilmiah Biologi dan Biologi Terapan yang memuat artikel-artikel ilmiah. Jurnal STIGMA diterbitkan oleh Program Studi Biologi-Fakultas Matematika dan Ilmu Pengetahuan Alam - Universitas PGRI Adi Buana Surabaya. Terbit dua kali dalam satu tahun (April-Juli dan September-Desember), Jurnal ini memfasilitasi penelitian dengan tema Biologi Umum, Biologi Terapan, Bioteknologi, Aplikasi Ilmu Biologi.
Arjuna Subject : Umum - Umum
Articles 163 Documents
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KEANEKARAGAMAN JENIS TUMBUHAN OBAT-OBATAN DAN PEMANFAATANNYA BERDASARKAN PENGETAHUAN MASYARAKAT LOKAL DI KECAMATAN MALINAU UTARA KABUPATEN MALINAU KALIMANTAN TIMUR Purity Sabila Ajiningrum
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 01 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no1.a588

Abstract

The objective of the study was to know local knowledge system of Dayak people in East Kalimantan. The data was collected using interview method to gather all information on plant diversity and it usage by the local society based on their own perception. The interview is using purposive sampling technique, conducted on the local society respondents whether male or female with a lifespan of 18 years or older. The number of respondents is 20% of the people in every sub district who took the Non Timber Forest Products (NTFPs) in the forest. The result of this research showed that many local people have a good knowledge about diversity surround them. Based on the results of interview, there were 31 plants species which were used as medication by the people. The factors relating to the cultural significance of plants would be discussed in detail in this article. Keywords: Timber Forest Products (NTFPs), Index of Cultural Significance (ICS), local society, interview
PENGARUH EKSTRAK BIJI PEPAYA (carica papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta indica A.Juss) DAN CAMPURAN EKSTRAK BIJI PEPAYA ( carica papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta indica A.Juss) TERHADAP DIAMETER TUBULUS SEMINIFERUS, SEL LEYDIG DAN BOBOT TESTIS MENCIT (Mus musculus) Apolonia M; Sukarjati sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 01 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no1.a634

Abstract

Papaya (Carica papaya L. ) and Neem (Azadirachia indica A.Juss) Tree are planted as an ornamental tree and have a character as a brush plant. They vegetated in tropical and subtropical region. The seed of papaya and Neem leaf are also include of a potential plants as a antifertility. Types of bioactif compounds contained in plants, the main compounds that are derived from the steroids, alkaloids, isoflavanoid, tripernoid, and activity as xanthon antifertilities. This encourages the researcher to know the influence of ethanol seed papaya extract and neem leaf extract towards diameter of seminiferous tubule, leydig cell and the testicular weight mice (Mus musculus). The sample of this research is the mice by as much as 21 tail with a weight of 20-30 grams, aged 2.5 months. Murine in to 3 groups, each group is divided 7 treatment. As for the treatments given are extracts of flowers, leaves, and roots of hibiscus with dose control, 50, and 100 mg/kg. Granting of extracts for 35 days. On day 37 mice are in surgery for testicular weight to observations taken, the volume of the testes. The testes then made preparations and measured diameter of seminiferous tubules. This research is experimental research using Random Complete Group. The data obtained were analyzed with variant analysis (ANOVA) in one direction and continued with test LSD (Least Significant Different). The results of the research there shows the influence of the ethanol papaya seed extract, meem leaf extracy and also the micture of papaya seed extract and neem leaf extract towards the weight of mice, leydig cell and the diameter of seminiferous tubule (P < 0.05). The best result to decrease the testicular weight and diameter of seminiferous tubule was showed by the neem with the weight of 100 mg/kg BB. The best result to decrease the leydig cell was showed on the mixture of papaya seed extract and neem leaf extract in dosage of 100 mg/kg BB. This study showed that papaya seed extract, neem leaf extract and the mixture of papaya seed extract and neem leaf extract able to decrease the testicular weight, diameter of seminiferous and leydig cell. In the next day, this research supposed to be able as an antifertility in male.
EFEKTIVITAS EKSTRAK DAUN MENGKUDU (Morinda citrifolia L.) DAN DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav) TERHADAP ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus F.E Afiff; Susie Amilah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 01 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no1.a635

Abstract

Has conducted research on the effectiveness of various concentrations of noni leaf extract (Morinda citrifolia L.) And red betel leaf (Piper crocatum Ruiz & Pav) the growth inhibition zone of staphylococcus aureus. Inhibition test using paper disc diffusion method. Paper discs soaked in noni leaf extract and red betel leaf for 30 minutes and placed on media that has been spread bacteria staphylococcus aureus and then incubated for 24 hours. The results showed that noni leaf extract and red betel leaves with a concentration of 40%, 60% and 80% affecting large growth inhibition zone of Staphylococcus aureus.
Pemberian Ekstrak Biji Alpukat (Persea americana Mill.) dan Biji Sirsak (Annona muricata L.) terhadap Mortalitas Larva Nyamuk Culex Sp S. Nombe; Diah Karunia Binawati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1027

Abstract

Vegetable insecticide is a natural material that is environmentally friendly. This material can be used in the prevention and control of mosquito larvae, especially Culex sp. Culex sp is the main vector of filariasis disease and dengue hemorrhagic fever (DHF). Use of natural materials is very good and environment friendly as insectcide compared with chemicals. Avocado seed extract (Persea americana Mill) is known to have secondary metabolite content of alkaloids, saponins, triterpenoids while soursop extract (Annona muricata L) in the form of acetogenin, squamocin and annonacin. This research needs to use of avocado seed extract (P. americana Mill) and soursop seed extract (A. muricata L) as larvacide of Culex sp. The aim of this research is to know the effect of avocado seed extract (P. americana Mill) and soursop seed extract (A. muricata L) to mortality of Culex sp Instar III mosquito larvae and LC50 for 24 hours. This study was an experimental study using a complete randomized design (RAL) with five treatment concentrations (0, 2, 4, 6, and 8%) avocado seed extract (P. americana Mill) and soursop extracts extract (A. muricata L) as much as five repetitions. The data obtained were analyzed by using One Way ANOVA to show the effect on the treatment. The results showed that giving of avocado seed extract (P. americana Mill) with 8% concentration (23,8 ± 0,45 larvae) was optimal in killing Culex sp mosquito larvae. While giving soursop seed extract (A. muricata L) with 4% concentration (25 ± 0,0 larva) optimal in killing Culex sp mosquito larvae. Avocado seed extract (P. americana Mill) has an effect as a larvacide against Culex sp. instar III with LC50 at concentration 2,486% while soursop extract (A. muricata L) with LC50 value at concentration 1,605%. Keywords: Larvacide, soursop seed extract, avocado seed extract, Culex sp
UJI BANDING LIMBAH CAIR IKAN, AZOLLA DAN LIMBAH KULIT PISANG SEBAGAI NUTRISI TAMBAHAN UNTUK KUALITAS PRODUKSI TANAMAN CABAI RAWIT (Capsicum frutencens L.) N. F. Rochmana; Ngadiani Ngadiani
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1028

Abstract

Household waste in the form of fish waste, azolla pinnata, and banana peel waste is a waste that has not been utilized optimally. The potential waste can be utilized as a raw material of organic nutrients with macro elements more complete than an-organic nutrients. Giving various variations of organic liquid nutrients can be used to chili (Capsicum frutencens L.). This study aims to determine the effect of different variations of organic liquid nutrient on the quality of chili production (Capsicum frutencens L.). The type of this research is quantitative research with experimental method using Completely Randomized Design consisting of 3 treatments 6 replications, so that there are 18 experimental units adn placed randomly was fish waste 20% (N1), 20% Azolla Pinnata (N2), and banana peel waste 20% (N3). Observations were made on three parameters of production quality is colour, taste, and wet weight. Technical of data analysis using One-way ANOVA with significance level of 0,05. One-way ANOVA test results on the quality of chili production is P>0,05. One-way ANOVA test results showed no signification on the quality of chili production (Capsicum frutencens L.). Keywords: Organic Liquid Nutrition, Liquid Waste Fish, Azolla Pinnata, Banana Peel Waste, Quality Productions of Plants, Chili.
PENGARUH ELISITOR Al3+ TERHADAP AKUMULASI DAIDZEIN DAN GENISTEIN PADA KALUS Phaseolus vulgaris L. Dan Phaseolus aureus Roxb. S. K. Dewi; W. Widoretno; Warsito Warsito
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1029

Abstract

The Estrogen was very beneficial hormone for men and women. Isoflavon compound was natural estrogen source which contain three main compound in chemical form, the aglycones, daidzein and genistein. They were found only 0,25% on plant, exclusively on leguminous plant. An alternative method have been required to overcome these problem, for example, through plant cell culture technic with heavy metal elicitor. The aims of this research were to study the effect of various concentration of Al3+ elicitor and age of culture for callus growth and accumulation of daidzein and genistein from in vitro Phaseolus vulgaris L. And Phaseolus aureus Roxb callus culture. Stable plant callus culture were established and subculture for three times approximately 4 weeks. The calli were elicitated by Al3+ elicitor with 0, 50, 150, 300 µM concentrations and incubated on 4, 6, and 8 weeks. Qualitative data were determined based in changed calli colour, TLC plate and value of Rf. Quantitative data were determined based in fresh and dry weight of the calli and the content of daidzein and genistein compound. Quantitative data were analized using Anova and continued with DMRT 95%. The results showed that the callus growth more influenced by age of culture. The sixth weeks led to higher fresh and dry weight. The Colour of Calli had been changed from white yellowness become brown at the eighth weeks. The texture of Calli have no difference between control and treatment of elicitor, that was friable until the end of treatment. The Calli with addition of elicitor Al3+ with 150 µM concentrations had significantly highest of fresh weight than control, they were over 0,463 g. The Content of daidzein and genistein on Phaseolus vulgaris L. had significantly increased in addition of Al3+ elicitor with 150 µM concentration. But, the content of daidzein and genistein on Phaseolus aureus Roxb. had significantly increased in addition of Al3+ elicitor with 300 µM concentration. Keywords: Phaseolus vulgaris L., Phaseolus aureus Roxb., elicitor Al3+, Daidzein, Genistein, Callus Growth.
KONSENTRASI HAMBAT MINIMUM EKSTRAK ETHANOL DAUN BELUNTAS (Pluchea indicha) DAN DAUN KEMANGI (Ocimum sanctum L) SERTA CAMPURAN DAUN BELUNTAS DAN DAUN KEMANGI TERHADAP PERTUMBUHAN BAKTERI (Escherichia coli, Staphylococcus aureus) PADA PENDERITA PROSTATITIS Isnainy Hidayati; Sukarjati Sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1030

Abstract

Prostatitis is a disease caused by bacteria, especially (E. coli, S. aureus). Treatment of diseases using antibiotics cause negative effects on the environment, pathogenic bacterial resistance, and antibiotic residues. Therefore, it is necessary to develop an antibacterial alternative from natural ingredients for the treatment of this disease. One of the natural ingredients that are antibacterial is basil leaf extract and beluntas leaves that have antibacterial inhibitory power. This research aims to prove the antibacterial activity of basil leaf extract and beluntas leaves in vitro. The treatments used were P0 (control), P1 (1.56%), P2 (3.125%), P3 (6.25%), P4 (12.5%), P5 (25%), P6 (50% ), P7 (100% ).. The parameters observed were bacterial growth on culture medium. The result of MIC (the lowest concentration of dilution that can inhibit bacterial growth) is seen from the treatment media turbidity and MBC result (the lowest concentration of dilution that can kill bacteria) seen from bacterial colony growth on NAP media. Data were analyzed descriptively. The results showed that basil leaf extract and beluntas leaves can inhibit growth or killing (E. coli, S. aureus) in prostatitis patients. Keywords: Prostatitis, antibacterial activity, microbial inhibition
AKTIVITAS ANTI FUNGI PIGMEN MERAH Penicillium purpurogenum TERHADAP Fusarium oxysporum S. Widayati; Tatang Sopandi
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1031

Abstract

Penicillium purpurogenum is known to be producing red pigment, pigment produced microbial activity, this research aims to demonstrate the activity of red pigment Penicillium purpurogenum antifungi against diameter and number of Fusarium oxysporum colonies, research done in the laboratory of Microbiology Adi Buana Surabaya University. This study used a randomized complete design (RAL) and Penicillium purpurogenum red pigment treatment concentrations of 0%, 0.5%, 1.0%, 1.5% and Ciprofloxacin 1.0%. Each treatment was repeated 4 (four) times, the research was carried out in vitro on medium PDA. The results showed that the red pigment resistance power P. purpurogenum 1.5% a significant effect (P < 0.05) against growth of fungi F. oxysporum and the number of colonies of fungis F. oxysporum compared red pigment resistance power P. purpurogenum 0% and 0.5%. growth of fungi F. oxysporum on PDA media containing pigment red P. purpurogenum 1.5% significant (P < 0.05) smaller than fungi growth F. oxysporum on PDA media containing pigment red P. purpurogenum 0% and 0.5%. Research results can be concluded that use of red pigment of P. purpurogenum can inhibit growth and the number of colonies of fungis F. oxysporum. Keyword: Red pigment, Penicilliumpur purogenum, Antifungi, Fusarium oxysporum.
Pertumbuhan Dan Kadar Klorofil Tanaman Pakcoy (Brassica rapa L.) Terhadap Cekaman NaCl V. Andriani
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1032

Abstract

Gravity salt is a soil condition with excessive amounts of soluble salt and adversely affecting plant growth. A number of agricultural crops are able to survive in areas with high salt. This study aims to determine the growth and content of packed chlorophyll packed NaCl at the beginning of the vegetative phase, and determine how optimal concentration in the growth of pakcoy. The research was conducted in experimental garden and basic laboratory of Faculty of Mathematics and Natural Sciences UNIPA Surabaya. The research design was using Random Random Complete with NaCl stress treatment variation. Variation of low NaCl stress treatment consisted of control watered with water (N0), initial NaCl 50 mM + continued 150 mM (N1), initial NaCl 75 mM + continued 150 mM (N2), initial NaCl 100 mM + continued 150 mM (N3), initial NaCl 125 mM + continued 150 mM (N4), and initial NaCl 150 mM + continued 150 mM (N5). Parameters observed were plant growth (plant height, leaf number, leaf width, and root length and biochemistry (leaf chlorophyll) .The quantitative data obtained were analyzed using ANOVA and followed by DMRT test at 95% confidence level.The results showed that the NaCl stress treatment low at the beginning of the vegetative phase raising the height of the plant, the number of leaves, leaf width, root length and the highest leaf chlorophyll content in N1. Keywords: NaCl stress, pakcoy, growth, chlorophyll, vegetative phase
PENGARUH LAMA PENYIMPANAN INFUSED WATER LEMON (Citrus limon) dan MENTIMUN (Cucumissativus L) TERHADAP PERTUMBUHAN BAKTERI M. I. Chandra; Susie Amilah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1033

Abstract

This study aims to determine the effect of long storage of infused water lemon (Citrus limon) and cucumber (Cucumissativus L) on bacterial growth. Infused water will be stored for 0 hours, 12 hours, 24 hours, 36 hours, and 48 hours at room temperature. This research uses experimental method. The sample used in this research is infused water lemon and infused water cucumber. The research was conducted in Bacteriology laboratory of Surabaya Health Analyst Department in January 2017 - March 2017. Data analysis was done by observing bacterial colony using total plate number by comparing the two sample treatments. Then proceed with statistical test Independent sample t-test about colony calculation results on infused water lemon and infused water cucumber. The final results show that there is an increase in the number of bacteria in the infused water lemon and infused water cucumber every hour. This increase in the number of bacteria can occur due to improper infusion of water storage, and several factors affecting infused water damage. Keywords : Infused water lemon, infused water cucumber, storage duration, bacterial growth, Total Plate Number.

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