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Ivandini Tribidasari A.
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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 11 Documents
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Search results for , issue "Vol. 16, No. 1" : 11 Documents clear
Modification of Gold Nanoparticles at Carbon Electrodes and the Applications for Arsenic (III) Detections Ivandini, Tribidasari Anggraningrum; Wijaya, Lany; Gunlazuardi, Jarnuzi
Makara Journal of Science Vol. 16, No. 1
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Cultivation of Uncultured Bacteria of the Class Ktedonobacteria in the Phylum Chloroflexi Yokota, Akira
Makara Journal of Science Vol. 16, No. 1
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The class Ktedonobacteria in the phylum Chloroflexi is known to contain a large number of uncultured, environmental 16S rRNA gene clones, and cultured representatives are a limited number. In this review, recent findings on the taxonomical and ecological significance of the class Ktedonobacteria in the phylum Chloroflexi are discussed based on the findings from both the characteristics of the cultured Ktedonobacteria and molecular-based analysis. The microorganisms characterized so far include four species in three genera, Ktedonobacter, Thermosporothrix and Thermogemmatispora, and were proposed to represent three families, Ktedonobacteraceae, Thermosporotricaceae, and Thermogemmatisporaceae, and two orders, Ktedonobacterales and Thermogemmatisporales. Ktedonobacteria strains showed a common property of gram-positive, aerobic organisms that produce branched vegetative mycelia and form spores by budding.
PMN Leukocytes and Fibroblasts Numbers on Wound Burn Healing on the Skin of White Rat after Administration of Ambonese Plantain Banana Aryenti, Aryenti; Suryadi, Suryadi; Harijadi, Harijadi; Yuhernita, Yuhernita
Makara Journal of Science Vol. 16, No. 1
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A study of ambonese plantain banana (Musa paradisiaca var sapientum Lamb) treatment in burn wound healing on the skin of white rats (Rattus novergicus) has been conducted. The wound healing of burn injuries was evaluated by counting the number of PMN leukocytes and fibroblasts at the 7th, 14th, and 21st days following the treatment. The study showed that the decrease in number of PMN leukocytes of subjects treated with ambonese plantain banana was relatively more significant compared to both negative and positive control (Bioplacenton ®). In contrast, an increasing number of fibroblasts was significantly demonstrated at the 14th and 21st days after treatment. In conclusion, ambonese plantain banana treatment in burn injuries will provide better results compared to both positive and negative controls.
Screening Antimicrobial Activity of Actinomycetes Isolated from Raja Ampat, West Papua, Indonesia Nurkanto, Arif; Julistiono, Heddy; Agusta, Andria
Makara Journal of Science Vol. 16, No. 1
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Penapisan Aktivitas Antimikroba dari Actinomycetes yang Diisolasi dari Raja Ampat, Papua Barat, Indonesia. Dalam rangka pencarian aktivitas antimikroba dari aktinomycetes di Papua, sebanyak seratus isolat Actinomycetes yang berasal dari tanah dan serasah dari beberapa ekosistem di Pulau Batanta dan Salawati, Papua Barat telah diuji. Sebanyak 200 ekstrak dari 100 isolat Actinomycetes telah diperoleh melalui dua tahap ekstraksi. Metabolit non polar diekstraksi menggunakan pelarut etil asetat : metanol (4:1), sedangkan metabolit polar diperoleh dari pemekatan medium menggunakan metode kering beku. Berdasarkan hasil pengujian menggunakan metode difusi agar, sebanyak 43 dari 200 ekstrak (21,5%) memiliki aktivitas antimikroba terhadap bakteri dan khamir (Escherichia coli NBRC 14237, Bacillus subtilis NBRC 3134, Staphylococcus aureus NBRC 13276, Micrococcus luteus NBRC 1367, Candida albicans NBRC 1594, dan Saccharomyces cerevisiae NBRC 10217). Hasil penelitian menunjukkan beberapa ekstrak Actinomycetes memiliki aktivitas anti bakteri gram negatif (1,5%), anti bakteri gram positif (17%), dan anti fungi (17%). Metabolit yang diekstraksi dengan pelarut etil asetat : metanol lebih aktif (35%) dibandingkan dengan pelarut air (17%). Sebanyak lima isolat yang memiliki aktivitas antimikroba tertinggi (BL-13-5, BL-06-5, BL-14-2, BL-22-3, dan Sl-36-1) diidentifikasi berdasarkan data sekuen gen 16S rRNA. Berdasarkan hasil pencarian homologi dengan program BLAST, diperoleh homologi spesies berturut-turut adalah Streptomyces kanamyceticus (92%), Streptomyces verne (92%), Streptomyces narbonensis (92%), Streptomyces malachitofuscus (98%), dan Streptomyces hygroscopicus (96%).
Earthquakes Sources Parameter Estimation of 20080917 and 20081114 Near Semangko Fault, Sumatra Using Three Components of Local Waveform Recorded by IA Network Station Santosa, Bagus Jaya; Madlazim, Madlazim
Makara Journal of Science Vol. 16, No. 1
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The 17/09/2008 22:04:80 UTC and 14/11/2008 00:27:31.70 earthquakes near Semangko fault were analyzed to identify the fault planes. The two events were relocated to assess physical insight against the hypocenter uncertainty. The data used to determine source parameters of both earthquakes was three components of local waveform recorded by Geofon broadband IA network stations, (MDSI, LWLI, BLSI and RBSI) for the event of 17/09/2008 and (MDSI, LWLI, BLSI and KSI) for the event of 14/11/2008. Distance from the epicenter to all station was less than 5°. Moment tensor solution of two events was simultaneously analyzed by determination of the centroid position. Simultaneous analysis covered hypocenter position, centroid position and nodal planes of two events indicated Semangko fault planes. Considering that the Semangko fault zone is a high seismicity area, the identification of the seismic fault is important for the seismic hazard investigation in the region.
The Genetic Diversity of Endophytic and Phyllosphere Bacteria from Several Indonesian Herbal Plants Soka, Yogiara Susan; Magdalena, Stella; Rachelia, Devi
Makara Journal of Science Vol. 16, No. 1
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Herbal plants have been believed by Indonesians to be an alternative medicine to treat illnesses. The bioactive compounds in the plant can be derived from secondary metabolites or from endophytic and phyllosphere bacteria which coexist within medicinal plants. A total of 18 endophytic bacteria and 32 phyllosphere bacteria were isolated from the herbal plants of Citrus sp., Pluchea indica, Curcuma longa, Nothopanax scuttelarium, Piper crocatum, and Andrographis paniculata. About 72% of endophytic bacteria isolates have proteolytic activity and about 11% have lipolytic activity. On the other hand, about 59% of phyllosphere bacteria isolates have proteolytic activity and about 19% have lipolytic activity. Phylogenetic diversity analysis was conducted by using the amplified ribosomal DNA restriction analysis (ARDRA) method and the sequence of 16S rDNA was digested with endonuclease restriction enzymes: MspI, RsaI, and Sau961. The diversity of endophytic and phyllosphere bacterium from the samples of herbal plants was high. Bacteria isolated from the same herbal plant does not always have a close genetic relationship except for the bacteria isolated from the P. indica plant which showed a close genetic relationship with each other
The Influence of Cadmium Heavy Metal on Vitamins in Aquatic Vegetables Widowati, Hening
Makara Journal of Science Vol. 16, No. 1
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The absorption of cadmium heavy metal in 3 (three) kinds of aquatic vegetables; water lettuce (Limnocharis flava), water convolvulus (Ipomoea aquatic Forsk.) and watercress (Nasturtium officinale R. Br) was studied to determine the influence of the contents of vitamins A and C. The purpose of this research was to identify the accumulation of cadmium in the organs of the aquatic vegetables, and their influence to the contents of vitamins A and C. This research was conducted by employing the factorial experimental design of randomized block design (RBD) with 3 (three) factors. The data was analyzed by regression to detect the correlation and contribution of cadmium influence on the aquatic vegetables. The accumulation of cadmium in the 3 (three) aquatic vegetables was mainly occured in the stems and leaves. Water lettuce has the highest accumulation of cadmium, followed by water convolvulus and watercress has the least. Cadmium is responsible for the declining levels of vitamins A and C.
Isolation of Antioxidant Compound from Endophytic Fungi Acremonium sp. from the Twigs of Kandis Gajah Elfita, Elfita; Muharni, Muharni; Munawar, Munawar
Makara Journal of Science Vol. 16, No. 1
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Sequential Isolation of Saturated, Aromatic, Resinic, and Asphaltic Fractions Degrading Bacteria from Oil Contaminated Soil in South Sumatera Munawar, Munawar; Aditiawati, Pingkan; Astuti, Dea Indriani
Makara Journal of Science Vol. 16, No. 1
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Sequential isolation has been conducted to obtain isolates of saturated, aromatic, resin, and asphaltene fractions degrading bacteria from oil contaminated sites. Five soil samples were collected from South Sumatera. These bacterial isolates were obtained using soil extract medium enriched with oil recovery or remaining-oil recovery degradated (ROD) as sole carbon and energy sources according to the isolation stage as the isolation medium. ROD at the end of every isolation stage analyzed oil fractions by use of the SARA analysis method. Six isolates of bacteria have been selected, one isolate was fraction saturates degrading bacteria that are Mycobacterium sp. T1H2D4-7 at degradation rate 0.0199 mgs/h with density 8.4x106 cfu/g from stage I. The isolate T2H1D2-4, identified as Pseudomonas sp. was fraction aromatics degrading bacteria at accelerate 0.0141 mgs/h with density 5.1x106 cfu/g are obtained at stage II. Two isolates namely Micrococcus sp. T3H2D4-2 and Pseudomonas sp. T1H1D5-5 were fraction resins degrading bacteria by accelerate 0.0088 mgs/h at density 5.6x106 cfu/g and 0.0089 mgs/h at density 5.7x106 cfu/g are obtained at stage III. Isolation of stage IV has been obtained two isolates Pseudomonas sp. T4H1D3-1and Pseudomonas sp. T4H3D5-4 were fraction asphaltenes degrading bacteria by accelerate 0.0057 mgs/h at density 5.6x106 cfu/g and accelerate 0.0058 mgs/h at density 5.7x106 cfu/g.
Self-Assembly and Hydrogelation of Peptide Amphiphiles Irwansyah, Irwansyah; Sihombing, Riwandi; Suwarso, Wahyudi Wahyudi
Makara Journal of Science Vol. 16, No. 1
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Seven peptide amphiphiles were successfully synthesized using solid phase peptide synthesis method. Peptide amphiphiles were characterized using Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometer (MALDI-TOF MS). Atomic force microscopy (AFM) study showed that peptide amphiphiles having glycine, valine, or proline as linker, self-assembled into 100-200 nm nanofibers structure. According to our research, both peptide amphiphile with positive and negative charges bear similar self-assembly properties. Peptide amphiphile also showed its capability as low molecular weight gelator (LMWG). Peptide amphiphiles bearing C-16 and C-12 as alkyl showed better hydrogelation properties than C-8 alkyl. Five out of seven peptide amphiphiles have minimum gelation concentration (MGC) lower than 1% (w/v).

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