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Journal of Biodiversity and Biotechnology
Published by Universitas Sebelas Maret
ISSN : 27755223     EISSN : 28083229     DOI : https://doi.org/10.20961/jbb
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, biotechnology, agriculture biotechnology, food biotechnology, medical biotechnology, bioinformatics, molecular biology, biochemistry, bioinformatics, phyto-pharmacy, herbal medicine, and animal science. Other topics are welcome if it discusses about biodiversity or biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Agronomi dan Ilmu Tanaman
Articles 6 Documents
 1 
Search results for , issue "Vol 4, No 2 (2024)" : 6 Documents clear
Morphological Character of The Natural Orchid Phalaenopsis Spp. Results of Colchicine Polyploidy Induction Sri Hartati; Samanhudi Samanhudi; Endang Suprapti
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i2.108518

Abstract

Induction of polyploidy in orchids can cause morphological changes such as larger leaf and flower sizes, longer bloom durations, and sharper flower colors. Morphological characterization needs to be done to determine the morphological changes that occur after colchicine treatment. This study aims to determine the morphological characters of natural orchids Phalaenopsis spp. polyploidy induction using colchicine. The research was carried out from November 2021-February 2022 at the Screen House, Faculty of Agriculture, UNS. The plant materials used included P. violacea from Sulawesi, P. violacea from Java, and P. Ambilis from Ambon. The treatment used was colchicine 1,250 ppm which was applied to flower buds using the cotton method with 2 replications. The collected data were analyzed by descriptive analysis and paired T-test using IBM SPSS Statistics 26 to compare the average performance of mutant plants with control plants. Induction of polyploidy using colchicine with a concentration of 1,250 ppm could increase the size of leaves, flower stalks, flowers, dorsal and lateral sepals, and petals in P. violacea from Java, P. violacea from Sulawesi, and P. amabilis from Ambon.
EFFECT OF WATERING FREQUENCY AND ORGANIC FERTILIZER ON THE GROWTH AND YIELD OF GROUND NUT Supriyono Supriyono; Berliana Nur Rizkyah; Maria Theresia Sri Budiastuti; Desy Setyaningrum
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i2.109410

Abstract

Groundnut (Arachis hypogaea) is one of the commodities with high economic value; however, weather anomalies can affect plant water availability, which in turn impacts groundnut production and productivity. If water availability is not balanced with proper fertilizer application, plant growth may be inhibited. This study aims to determine the role of watering frequency and organic fertilizer on the growth and yield of ground nut. The research was conducted at the Laboratory of the Faculty of Agriculture, Sebelas Maret University, located in Sukosari Village, Jumantono District, Karanganyar Regency for a duration of four months, from December 2024 to March 2025. The method used was a factorial Randomized Complete Block Design (RCBD), consisting of two factors and three replications. The first factor was watering frequency (once a day, once every two days, once every three days, and once every four days). The second factor is the organic fertilizer dosage (0; 10; 20; and 30 tons.ha -1). Data were analyzed using Analysis of Variance (ANOVA) at a 95% significance level, followed by Duncan’s Multiple Range Test (DMRT) at a 95% significance level. Regression analysis was performed to determine the recommended dosage, and correlation analysis was used to identify relationships between observed variables. The result showed that watering once every three days resulted in the best 100-seed weight. Watering once a day resulted in the best dry biomass weight. Organic fertilizer dose of 20 t.ha-1 produced the best plant height, leaf area, leaf area index, number of pods, fresh pod weight, dry pod weight, number of seeds, and seed weight. Organic fertilizer dose of 30 t.ha -1 produced the best number of leaves at 5 Weeks After Planting (WAP), number of nodes at 5 WAP, and flowering age. Interaction between watering once a day and organic fertilizer dose of 20 t.ha-1 produced the best dry biomass weight.
Effect of Goat Manure and Moringa Leaf Extract on Vegetative Growth of Robusta Coffee (Coffea canephora) Fitria Roviqowati; Muhammad Farhan Alfianto; Pardono Pardono; Edi Purwanto; Ahmad Yunus
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i2.106012

Abstract

The use of organic fertilizers and growth stimulants in the form of natural PGR is the main solution to support sustainable coffee cultivation. This research aims to determine the best treatment combination, the best dose of goat manure, and the best concentration of moringa leaf extract for vegetative growth of robusta coffee TBM 1. The research was conducted in Kemuning Village, Ngargoyoso District, Karanganyar Regency in October 2023-April 2024. The method used was a Randomized Complete Group Design (RCBD) consisting of two factors and three replications. The first factor was goat manure (0, 3, 4, and 5 kg.plant-1) . The second factor was moringa leaf extract (0, 150, 200, and 250 g.L-1concentration). Data analysis used analysis of variance (ANOVA) at the 5% error level then continued with Duncan's Multiple Range Test (DMRT) at the 5% error level if there were significant results. The observed variables include plant height, stem diameter, number of leaves, number of branches, leaf area, and total chlorophyll. The results showed that the application of goat manure and moringa leaf extract affected the vegetative growth of robusta coffee plants. The combination of goat manure 4 kg.plant-1and moringa leaf extract at a concentration of 250 g.L-1had an effect on increasing chlorophyll b and total chlorophyll. Goat manure had no effect on all variables. Without the moringa leaf extract had better effect on plant height and the moringa leaf extract concentration of 250 g.L-1affected the increase in leaf area 6 weeks after application.
Effect of Concentration and Time of Chitosan Application on The Growth of Pineapple (Ananas comosus) Endang Yuniastuti; Salsabila Tiara Putri; Gani Cahyo Handoyo
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i2.108238

Abstract

Pineapple (Ananas comosus) is a popular fruit crop in Indonesia. Pineapple crowns are one of the planting materials used in pineapple cultivation, but their growth is slower than other planting materials, so that the addition of chitosan is needed, which has the potential to stimulate growth. This study aims to determine the best concentration and application time of chitosan that can improve pineapple growth. The study was conducted in the screenhouse of the Faculty of Agriculture, Sebelas Maret University, Surakarta during March-June 2025 with a Completely Randomized Design (CRD) with two factors, namely chitosan concentration (without chitosan, 1 ppm, 3 ppm, 5 ppm) and time of chitosan application (morning, afternoon, morning and afternoon). Each treatment was repeated 4 times, resulting in 48 experimental units. The results showed that no combination of concentration and time of chitosan application was found to simultaneously increase pineapple growth. The treatment concentration of 1 ppm and 3 ppm gave the best result to increasing leaf number; concentration 1 ppm, 3 ppm, and 5 ppm gave the best result to increasing total leaf area and longest root length. Time of chitosan application once in the morning or evening gave the best result to increasing photosynthesis rate and stomatal conductance. Chitosan has the potential as a biostimulant for plant growth and is effective in increasing the physiological activity of plants.
Yield Performance of Short-Stem Promising Mutant Lines of Mentik Wangi Rice Induced by 200 Gy Gamma Irradiation Fitria Roviqowati; Desfinenda Calistarajni; Parjanto Parjanto; Edi Purwanto; Ahmad Yunus
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i1.106011

Abstract

The demand for rice in Indonesia continues to increase but production has decreased. Efforts that can be made are the use of local varieties of mentik wangi rice. Mentik wangi rice has the disadvantages of long life, high stems, and low crop production. As a solution, plant breeding is carried out in the form of mutation by giving gamma irradiation which can improve unwanted genetic traits and produce superior varieties with the dose used of 200 gray. This study aims to evaluate the yield potential of promising mutant strains with 200 gray gamma irradiation on mentik wangi rice and get mutant strains that have superior criteria for short-stemmed, short-lived, and high-yielding. This research was conducted in the rice field of Ngampel Hamlet, Gentungan Village, Mojogedang, Karanganyar, Central Java. The research was conducted in June-October 2023. The design used was a Randomized Complete Group Design (RCBD) with a single factor, namely strains consisting of 14 mutant strains and 1 control strain with 3 repetitions. The results of observations were analyzed by Analysis of Variance (ANOVA) at the 5% level and continued with Duncan Multiple Range Test (DMRT) at the 5% level. All tested strains produced the expected superior criteria, namely short stem, short age, and high productivity.
APPLICATION OF BENZYL ADENINE AND IAA ON THE GROWTH OF BANANA BARANGAN SUBCULTURE (Musa acuminata L.) Andriyana Setyawati; Samanhudi Samanhudi; Edi Purwanto; Retna Bandriyati Arniputri; Kevin Putra Setyawan; Muhammad Aji Cahyadi
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i2.108497

Abstract

Barangan banana is a banana variety that has high economic value, but its production is still carried out conventionally, resulting in limited barangan bananas on the market. The use of tissue culture methods can quickly produce high-quality, disease-free plants regardless of seasonal conditions. The aim of this research is to determine the effect of providing the best ZPT IAA and BA for the growth of the Barangan banana planet. The research was conducted in August-October 2023 at the Biotechnology and Plant Physiology Laboratory, Sebelas Maret University. This research used a two-factor Completely Randomized Design (CRD) with 6 replications. The first factor was the use of ZPT Indole Acetic Acid (concentrations 0, 0.5, 1, and 1.5 ppm and the second factor was the use of ZPT Benzyl Adenine (0, 0.5, 1, 1.5 ppm). The variables observed were root emergence time, number of roots, root length, time of leaf emergence, number of leaves, time of shoot emergence, number of shoots, and height of shoots. The observed data were analyzed using Analysis of Variance (ANOVA) with a 5% level test and if there was a significant difference, it was continued with Duncan's Multiple Range Test (DMRT) at the level 5%. The results showed that giving 0.5 ppm IAA increased root length; 0.5 ppm IAA was given optimally for root formation; and 1 ppm IAA increased the shoot height of the Barangan banana plnalet. Giving BA 0.5 ppm root number; BA 1, 5 ppm produces the highest number of leaves; BA 1 ppm increases shoot height of barangan banana plantlets.

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