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Journal of Global Pharma Technology
Published by Universitas Udayana
ISSN : 09758542     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and citations to articles published in JGPT. JGPT publishes original research work that contributes significantly to the scientific knowledge in pharmacy and pharmaceutical sciences- Pharmaceutics, Novel Drug Delivery, Pharmaceutical Technology, Cosmeticology, Biopharmaceutics and Pharmacokinetics, Pharmacognosy, Natural Product Research, Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmaceutical Analysis, Pharmacology, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics, Biotechnology and Applied Computer Technology. For this purpose we would like to ask you to contribute your excellent papers in pharmaceutical sciences.
Arjuna Subject : Kedokteran - Onkologi
Articles 2,439 Documents
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Determination of Mefenamic Acid using 8-hydroxy quinoline as a Precipitating Agent and Low Pressure Mercury Lamp (184.9 & 253.7 nm) as a Source of Irradiation using of ISNAG Continue Flow Fluorimeter Hussein Fares Abd-Alrazack
Journal of Global Pharma Technology Volume 11 Issue 03
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Abstract

A new, simple, sensitive and fast method for the determination of Mefenamic acid in pure form and drugs (tablets) by continuous flow feed analysis via measurement of diverged beam of light by ISNAG_-fluorimeter (homemade instrument). The method based on the reaction of the Mefenamic acid with 8-HQ to form yellow precipitate. Optimum parameters have been studied to increase the sensitivity for the developed method. The linear dynamic range for the instrument response versus Mefenamic acid concentration was 0.005-7 mmol/L while the L.O.D was 0.216 µg/sample from the step wise dilution for the minimum concentration of lowest concentration in the linear dynamic range of the calibration graph. The correlation coefficient (r) was 0.9956 while percentage linearity (R2%) was 99.12%. RSD % for the repeatability (n=8) was lower than 0.2% for the determination of Mefenamic acid, with concentration of 2, 5 mmol/L respectively. The developed method was applied successfully for the determination of Mefenamic acid in pharmaceutical tablets. A comparison was made between the newly developed method with the classical method (UV-Vis spectrophotometry at wavelength 285nm, and Turbidemtric method) of analysis using the standard addition method via the use of paired t-test. It shows that there was no significant difference between the quoted values of each individual company with calculated t-value at 95% confidence interval from developed method.Keywords: Mefenamic acid, Flow injection analysis, Homemade instrument.
Cosmeticology: Disorders and its Advanced Treatment Gita Chaurasia
Journal of Global Pharma Technology Volume 02 Issue 10: (2010) Oct.2010
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Abstract

COSMETICOLOGY: DISORDERS AND ITS ADVANCED TREAT-MENT
Molecular Detection of Gardnerella Vaginalis Isolated from Preterm Labor Patients and Study of Some Factors on Biofilm Formation in Al-Hilla City, Iraq Ilham A. Bunyan
Journal of Global Pharma Technology Volume 09 Issue 12
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Abstract

Objectives: the present study aims to isolation and identification of G.vaginalis from preterm labor, detection of bio film formation of G.vaginalis and effect of some factor on bacterial adherence. Material and methods: One hundred fifty clinical sample were collected from preterm labor patients with (bacterial vaginosis, urinary tract infection and aborted women) 80, 15, 55 respectively, admitted to Babylon Maternity and pediatric hospital and Al-Hilla Teaching Hospital, at the period from February to October 2016. These high vaginal samples were subjected to different methods of identification of G.vginalis. Results and conclusion: It was found that 6 (4%) G.vaginalis isolates were recovered by using selective media where 30 (20%) isolates were recovered dependent on direct extraction to high vaginal swab on molecular level.  We  were  detection and found  that  (4) isolates were gave strong biofilm While the other Gave moderate then subjected to some factors to reduce , The effect (Alum ,sodium bicarbonate  and sodium chloride)  at concentration (50%) which gave results (100%) very effective to eradication bio film ,the antibacterial activity of  thymol plant extract (carvacrol) at concentration (50%) were investigated the results proved that is effective  on disrupted biofilm in (83.4%). The used of metronidazole were very effective on biofilm (100%)as well as in Alum ,sodium chloride and sodium bicarbonate The results showed that all factors below have the ability to destruction G.vaginalis biofilm .Keywords: G. vaginalis, cpn60, 16sRNA, bio film, Preterm labor. 
The Dominance of the ACE Gene Shapes According to Anaerobic Threshold for Players in Handball Donnie Sabah Ali
Journal of Global Pharma Technology .
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Abstract

The current study aimed at identifying the shape s of the ACE gene and the laconic threshold. The researchers used descriptive research in a survey method to identify the majority of players who had a delay in reaching the genetic threshold according to the gene, as were identified shape s of dominant gene in the research sample which (DD, ID, II) has been the research sample and selected determine purposively they handball players for the team Muthanna province's 12 players have been divided according to shape s to (DD) and the number (5 ) players and (II) and the number (4) players, and finally (ID) and the number (3) players have been conducting a blood test for the withdrawal of a sample corresponding to the date on Monday, a brief summary (14 \ 2 \ 2018). The gene was detected in the sample, after which the gene shape s were detected to identify the differences between the groups through any group that was delayed to reach fatigue and the group with the genetic makeup (DD) was the most delayed to reach the anaerobic threshold where researchers attribute the genetic makeup (DD) is what distinguishes this group from others.Keywords: ACE gene and anaerobic threshold.
Influence of Erythropoietin and Platelet-Rich Plasma on Cyclophosphamide-Induced Hepatotoxicity in Male Albino Rats Ahmed Hafidh
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

The present study was conducted to investigate the protective effects of erythropoietin (EPO) and platelet-rich plasma (PRP) against cyclophosphamide (CP)-induced hepatotoxicity. Thirty six male albino rats were divided into six equal groups. The first group (control).The 2nd group was injected with a single dose of EPO (3000 IU/Kg, i.p.). The 3rd group was injected with PRP extract (150 µl/Rat, s.c.) on days (0,5 and 10). The 4th group was injected with a single dose of CP (150 mg/Kg, i.p.). The 5th group was injected with (EPO+CP) and the 6th group was injected with (PRP+CP). At the end of the experiment (day 15) sera were collected for assessment of liver function enzymes (AST, ALT and ALP) and protein levels (total protein, albumin and globulin). The CP-induced hepatotoxicity was evidenced by increased serum AST, ALT, and ALP accompanied by reduction in the levels of total protein, albumin and globulin in the serum. Administration of EPO or PRP significantly reduced the liver toxicity. The results revealed that EPO and PRP have ameliorative effect against CP-induced hepatotoxicity in male albino rats. Keywords:  Cyclophosphamide, Erythropoietin, Platelet-Rich Plasma, Hepatotoxicity
Human Sperm Chromatin Maturation: A Study of Prolactin Supplementation in Sperm Preparation Silvia W Lestari
Journal of Global Pharma Technology Volume 12 Issue 06 (2020) June 2020
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Abstract

One of the management of infertile couples is assisted reproduction through the Intra Uterine Insemination (IUI). Paternal factor has been known to influence the success of IUI, such as sperm selection on preparation. Prolactin (PRL) is reported to select sperm with better DNA fragmentation on the preparation process. This study aimed to investigate sperm selection with better chromatin maturation quality, by the supplementation of PRL. Semen samples were obtained from 10 infertile men who experienced sperm preparation for IUI. Prepared sperm were incubated at 1000 ng/ml of PRL, followed by chromatin maturation test. Its index was determined as mature sperms in 100 sperms. The differences in sperm chromatin maturation showed a significant difference (p
The Effect of Inhalation Smoking by Narghile on Some Parameters Biochemistry Noor Essam Abdul Rezaq
Journal of Global Pharma Technology Volume 10 Issue 05: (2018) May2018
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Abstract

The present study included 50 sample collected  different of Iraq.25 sample narghile smoker men group  and 25 control men  group show the results increase significant in MDA, ALT, AST and LDH in narghile smoker men group  when compared with control men  group , while decrease significant in C.K and ACHE in narghile smoker men group  when compared with control men  group   Keywords: Nargile, Oxidative stress, MDA, LDH , CK , ACHE , ALP , ALT and AST.
Prevalence of Pregnant Women with Anemia in Sangkan Gunung Village, Karangasem Regency, Bali-Indonesia Luh Seri Ani; Ketut Suwiyoga
Journal of Global Pharma Technology Volume 09 Issue 03
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Abstract

Pregnant women are one of the groups with the risk of having anemia. Some factors that are correlated to anemia is an infectious disease, bleeding, and low iron daily intake.These factors were related to the knowledge level of pregnant women and iron supplementation compliance. This is a cross-sectional study with descriptive methods, where 34 pregnant women were chosen by consecutive sampling method. Anemia was then measured by sahli method, and hemoglobin level < 11 g/dl were categories as anemia. The results were analyzed with univariate and bivariate analysis using SPSS 23. Anemia in pregnant women at Sangkan Gunung village, Sidemen sub-district is20,6%.  Most anemia incidents were found in 20-35 years old pregnant women, with low education level, work at the office, zero parity, and in afirst trimester. Anemia was not associated with chronic energy deficiency, knowledge level and iron supplementation compliance level (P>0,05). Anemia prevalence of pregnant women was relatively low, but the preventive effort must be taken to decrease therate of anemia prevalence.Keywords: Knowledge, Compliance, Iron tablets, Pregnant women, Anemia.
Serum Levels of Cross-linked N-Telopeptide of Type I Collagen before and After Non-surgical Periodontal Therapy in Type 2 Diabetic Patients with Chronic Periodontitis Wael A. Kzar
Journal of Global Pharma Technology Volume 11 Issue 2: 2019
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Abstract

Background: Diabetes mellitus is a major risk factor for chronic periodontitis (CP) and hyperglycemia has an important role in the enhancement of the severity of the periodontitis. It has been reported that the progression of CP causes shifting of the balance between bone formation and resorption toward osteoclastic resorption, and this will lead to the release of collagenous bone breakdown products into the local tissues and the systemic circulation. Cross-linked N-telopeptide of type I collagen (NTx) is the amino-terminal peptides of type I collagen which is released during the process of bone resorption. This study was conducted to determine the effects of nonsurgical periodontal therapy on serum level of NTx in type 2 diabetic patients with chronic periodontitis (CP) and in systemically healthy patients with CP and to correlate NTx level with the clinical periodontal parameters in the studied groups. Materials and methods: The studied sample consisted of ninety subjects of both genders with an age range (35-55). They were divided into three groups, the first group consisted of 30 subjects with healthy periodontium and systemically healthy  (control group), the second group consisted of 30 patients having chronic periodontitis and type 2 diabetes mellitus (CPDM) group, and the third group consisted of 30 patients with chronic periodontitis only and systemically healthy (CP) group. All the subjects enrolled in this study were with normal body mass index. The clinical periodontal parameters measured were plaque index (PLI), gingival index (GI), probing pocket depth (PPD), and relative attachment level (RAL). The blood samples were collected from all individuals and examined to measure the serum level of NTx using enzyme-linked immune sorbent assay (ELISA). Patients with chronic periodontitis were treated with scaling and root planning (SRP) and recalled for further measurements of the clinical periodontal parameters and collection of the blood sample to measure the serum level of NTx after eight weeks. Results: All the clinical periodontal parameters at the baseline were higher than that after treatment. PPD and RAL were highest in CPDM group followed by CP. Regarding the level of NTx at the baseline; it was highest in CPDM group followed by CP group then the control group. After treatment, results revealed a reduction in NTx level for both CPDM and CP groups. Regarding the correlation between the clinical periodontal parameters and NTx level, the results revealed almost a non-significant weak positive correlation. Conclusions: SRP is an impactful procedure and results in the improvement of all the clinical periodontal parameters and reduction in the serum level of cross-linked N-telopeptide of type I collagen which could be considered as a good bone resorption marker in the studied groups.Keywords: Periodontitis, Type 2 diabetes mellitus, Cross-linked N-telopeptide of type I collagen, Nonsurgical periodontal therapy.
Expression of Endoglin in Bladder Carcinoma of Iraqi Patients Rawaa Al-Chalabi
Journal of Global Pharma Technology Volume 11 Issue 08 (2019) Aug. 2019
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Abstract

Angiogenesis is an important process for tumor growth in almost all malignancies. Endoglin (CD105) is a membrane glycoprotein, part of the TGF beta receptor complex, which strongly expressed on the surfaces of cancerous cells. It has a vital role in angiogenesis, tumor growth, survival and metastasis of cancer cells to other locations in the body. Methods: This study designed to evaluate the role of endoglin immune expression in the development of bladder carcinoma by using monoclonal antibody, 137 biopsies of bladder carcinoma were collected from patients and 42 normal bladder tissues were collected from forensic autopsy. Results: 118 (86.13%) out of 137 tumors and 27 (64.26%) of normal tissues gave positivity with different scores while other biopsies expressed negative staining pattern. Score +3 showed the highest frequency among the tumor tissues while normal bladder tissues expressed score+1 as the highest one. Conclusion: endoglin is a marker of angiogenesis closely associated with malignant potential and bladder carcinoma development.Keywords: Endoglin, Angiogenesis, Urinary bladder Carcinoma (UBC)

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