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Journal of Global Pharma Technology
Published by Universitas Udayana
ISSN : 09758542     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and citations to articles published in JGPT. JGPT publishes original research work that contributes significantly to the scientific knowledge in pharmacy and pharmaceutical sciences- Pharmaceutics, Novel Drug Delivery, Pharmaceutical Technology, Cosmeticology, Biopharmaceutics and Pharmacokinetics, Pharmacognosy, Natural Product Research, Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmaceutical Analysis, Pharmacology, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics, Biotechnology and Applied Computer Technology. For this purpose we would like to ask you to contribute your excellent papers in pharmaceutical sciences.
Arjuna Subject : Kedokteran - Onkologi
Articles 2,439 Documents
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Preparation a New SHE-Medium Replacement of RPMI1640-Medium using Oral Rehydration Solution Ekhlass N. Ali
Journal of Global Pharma Technology Volume 12 Issue 02 (2020) Feb. 2020
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Abstract

The study showed that the use of the new  culture medium which called (SHE-medium) prepared by using oral rehydration salts, led to increase  the  ratio of cell viability of PMNs and lymphocytes compare with RPMI 1640-medium on 24,48,72 h. The results shows that ratio of cell viability of PMNs in SHE-medium were 89%,91% ,93% respectively, while in RPMI 1640-medium were 80%,90%,93% respectively while the  ratio of cell viability of lymphocyte in SHE-medium were 88%,90% ,91% respectively, compare with RPMI 1640-medium which are 80%,85%,90% respectively. But when tested the effect of SHE-medium on THP-1 cell line and REF cells and comparison with RPMI 1640-medium, the results shows that the ratio of cell viability on SHE-medium were 84.33% and 74.33% respectively, while in RPMI 1640-medium were 86.67% and 80.67% respectively.Keywords: 1640-medium, SHE-medium, Oral rehydration solution.
The Relationships of Cytomegalovirus’s Infection among Pregnant Women with Some Variables Saadya Hadi Humade
Journal of Global Pharma Technology Volume 9 Issue 11
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Abstract

Back ground: CMV is Human Herpesvirus 5 (HHV-5). Belong to the ''Betaherpesvirinae'' subfamily of ''Herpesviridae'', which also includes Roseola virus. It leads to an initial CMV infection, with a few symptoms. The virus remains latent in the body, reactivating when the body’s immune system is weakened. CMV causes an estimated 400 deaths each year and permanent disabilities in about 8,000 children, according to statistics by the U.S. Centers for Disease Control and Prevention. A pregnant woman can pass CMV to her child and the results gave birth has severe cerebral palsy, hearing and vision loss. Treating a pregnant woman for CMV will lessen or prevent long term disabilities of the fetus in utero.  Objective: To find out the relationship between demographic variable and reproductive variable with the predisposing factors of the disease. Methodology: descriptive study done at Bable maternity hospital conducted, a purposive sample of (56) women how have Cytomegalovirus, from the period    1/4/2017 to 30/4/2017. Results: the study revealed that the high percentage for women who affected with CMV with in age group (16-25) years (41.1%). (33.9%) graduated from the primary school related reproductive variable,(94.28%) they have number (1-4) graveda, (92.8%) they have(92.8%)they have (1-4) para and (76.78%) they have(1-3) abortion.(78.6%) they aborted at 11-20 weeks gestation. Also (30.4%) they have fetal anomalies and (50%)at they didn’t taking medication during pregnancy. The study revealed that a highly significant correlation between age and education, occupation, residence, gravida, para and abortion, highly significant correlation between Gestational age and fetal anomalies and immune disease and highly significant correlation between fetal anomalies& immune disease. Conclusion: the study was concluded that the study sample don’t aware about CMV and its complication. Recommendation: Sequential ultrasound examinations should be performed every 2 to 4 weeks to detect sonographic abnormalities, to determine the prediction of the fetus and serologic testing for CMV for women who develop influenza-like illness during pregnancy.Keywords: Cytomegalovirus, fetal anomalies, abortion.
The Effect of Teaching Exercises by Means of Aids in Some of Biomechanical Variables and the Achievement of Shot Put for Girls Students Mayassa Abd Ali Kahdim Sebti
Journal of Global Pharma Technology .
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Abstract

The study aimed to identify the effect of educational exercises related to the aids in the development of some biomechanical variables and the achievement of the weight-effectiveness of the students. The study also hypothesis that there are statistically significant differences between the results of the tribal and remote tests of the experimental and control groups. The researcher used the experimental method (and the design of the two equal groups) for the suitability and the nature of the research on the research community of the first stage students (40) students in the Department of Physical Education and Sports Sciences / Faculty of Education for Girls / University of Kufa for the academic year (2016 - 2017) Randomization The sample was randomly divided and distributed by lottery method to two groups of 15 students for both experimental and control groups. After conducting the pre and posttests, the data were collected and processed statistically to extract the results through which the most important conclusions were reached:There are statistically significant differences between the results of the pretest and the posttest of the achievement of the shot put and the experimental and control groups, as well as when comparing the results of the tests of the control and experimental groups and for the benefit of the experimental group.The use of educational aids to teach the activity of the shot put and greatly develop the skill mentioned.The results showed that there were differences between the results of the post and pretests of the experimental research group, except for variable high aviation point, since there were no differences between the results of the pre and posttests.There were significant differences between the results of the pre- and post-test tests of the control group in the investigated biomechanical variables, while there were no significant differences in high aviation point.There were no significant differences between the two post-experimental tests of the experimental and control groups in variable high aviation point, while the biomechanical variables detected showed significant differences in favor of the experimental group.Keywords: Teaching exercises, Means of aids, Biomechanics and Shot put.
Role of Sequencing Genetic for 708 T>A of Cholesterol 7α Hydroxylase (CYPA17) in Patient with Gallstone Disease Antesar Rheem Obead
Journal of Global Pharma Technology Volume 11 Issue 6.
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Abstract

AIM: To investigated the association of the CYP7A1 gene polymorphism and analyze the sequencing of CYP7A1 gene   with development of gallstone disease in Iraq population. Methods: The polymorphism were analyzed by polymerase chain reaction followed by restriction fragment length polymorphism, in two group match by age and sex: patient with gallstone (n=140) and stone-free control (n=140).Result: Novelty single nucleotide   polymorphism   in upstream of   CYP7A1 gene   was founded for   PCR amplification   708 T >A in patient with gallstone and conformed by sequencing data and allelic frequencies in patient and control for T of CYP7A1 gene  38.2% vs 54.6% were significant (p=0.0001).Conclusion: The result have been demonstrated that association between the polymorphism of 708 T> A for CYP7A1 gene and an effect on cholesterol 7 α hydroxylase serum level with high prevalent of gallstone disease.Keywords: CYP7A1, Gallstone, Polymorphism, Sequencing analyzing.
The Development of Toddlers in Russia: Needs and Trends Mikhail Volchanskiy
Journal of Global Pharma Technology Volume 12 Issue 08
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Abstract

The level of physical development of children reflects their health status. The goal is to evaluate the anthropometric indicators of toddlers aged 1-3 in Moscow as a sample model of Russia and to identify gender and age differences. The studies were conducted in 2017-2019 in Moscow (Russian Federation). On the basis of 10 pediatric clinics, 3000 children were selected, of which 1650 boys and 1350 girls of toddler age, 1-3 years old. 4 anthropometric parameters were regularly measured (body length, body weight, circumference of the chest and head). Significant differences in all anthropometric parameters were found to be large for boys compared to girls. In boys, there was a 9.0 cm difference in body length between 2 and 1 years (p ≤ 0.001), and 10.0 cm between 3 and 2 years (p ≤ 0.001). In girls, the results were identical (p ≤ 0.001). Boys aged 2 were 2.6 kg heavier than one-year-olds (p ≤ 0.001), and at the age of three they were 1.8 kg heavier than two-year-olds (p ≤ 0.001). Girls aged 2 had body weight 2 kg more than one-year-olds (p ≤ 0.001), and 3-years-olds were 1.3 kg (p ≤ 0.01) heavier compared with two-years-olds. The chest circumference in boys aged 2 is 2.5 cm larger than in one-year-olds, and 1.5 cm (p ≤ 0.001) smaller than in toddlers aged 3. In girls the differences were 2.5 cm and 1.2 cm, respectively (p ≤ 0.01). The head circumference for boys aged 2 was 2 cm more than the parameters of one-year-olds (p ≤ 0.001), and for boys aged 3 - 1 cm more than two-year-olds (p ≤ 0.001). For girls the differences were 2 cm between ages of 1 and 2, and 1 cm between ages of 2 and 3 (p ≤ 0.001). The research results can be adapted when creating a single database of world data to identify the processes of acceleration or deceleration and the factors that determine them. The processes of changing anthropometric indicators were identified for toddlers aged 1-3. Three anthropometric indicators (head and chest circumference, body length) in boys and girls change with the same growth rate over time. The fourth indicator, body weight, changes somewhat faster in boys (0.8 kg versus 0.7 kg in girls, p ≤ 0.05). The remaining three indicators significantly differ within groups of boys and girls between age groups (p ≤ 0.001). Thus, age differences prevail over gender differences. Keywords: Early childhood, Regional standards, Socio-economic factors, Anthropometry, Body weight, Body length, Chest circumference, Head circumference.
A Forensic Study of Mycosis and its Relation ‎with Amphetamine Addiction Tests Suhailah M. Wahhoodee
Journal of Global Pharma Technology Volume 10 Issue 05: (2018) May2018
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Abstract

The abuse of amphetamines is of national concern from a public health perspective. This issuing a nowadays important subject, because the problem of amphetamine drugs has recently been raised toa very dangerous level. Amphetamines are generally prescribed as a potent central nervous system (CNS) stimulant that is used in the treatment of attention deficit hyperactivity disorder (ADHD), narcolepsy, and obesity. The present study aimed to test the ability of Fungi to bio-convert methamphetamine that may change the metabolite pattern and / or concentration of the drug. Total of 53 blood samples were collected (10 samples from drug addict prisoners at Al-Samawa prison, 30 samples from healthy (non-symptomatic), 13 sample phlegm of respiratory system patients). Samples were cultured on Blood agar then purified on Sabouraud dextrose agar. Thereafter, each isolate was cultured in 5ml Sabouraud dextrose broth containing 6mg/ml methamphetamine drug and incubated at 37ºC. Samples were tested periodically, by thin layer chromatography (TLC) using methanol and ammonia (100:1.5) as a mobile phase. The separated spots were visualized by spraying with iodoplatinate or Marquis Reagent the results revealed that 11 sample was able to convert the drug after 14 days of incubation. The TLC method found more advantageous in detecting drug bio-conversion. Add to that, it is inexpensive, fast and easy to manipulate in forensic labs.Keywords: Methamphetamine, Fungi, TLC.
Antimicrobial Resistance among Escherichia Coli Causing UTIs in Women Hawraa Wahab Aziz; Israa Adnan Ibraheam; Hawraa Sabah Almusawi; Zeana AL Hindi; Tsahel H. Al-Dulaimi
Journal of Global Pharma Technology Vol. 9 Issue 7 : 2017
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Abstract

Urinary tract infections (UTIs) are common bacterial infections, particularly in women. E. coli is  one of the most frequently isolated  bacteria in (UTIs), , it also caused of childhood diarrhea, severe anemia, ,kidney failure, pneumonia, sinusitis and endocarditis. The current study tried to detecting Antimicrobial Resistance Among Escherichia coli Causing UTIs in women. Of the total 48 isolates of Escherichia coli, very high rates of resistance was seen with Ticarcillin 48 (100%), Piperacillin48 (100%), Aztreonam 46 (95.8%), Cefepime and 44 (91.6%)  Ceftazidime 44 (91.6%) . High rates of sensitive was seen with Imipenem 48 (100%), Meropenem 48 (100%), Amikacin 46(95.8%) , Gentamicin 32 (66.6%) , Piperacillin\Tazobactam 32(66.6%), Tobramycin 28 (58.3%) and ciprofloxacin 26(54.1%) , while of Minocycline 8 (16.6 %) has intermediate susceptibility to antibiotics study .According to the last results  the most effective antibiotics were Meropenem , Imipenem and Amikacin so they are considered the best drugs used for treatment of UTIs which caused by Escherichia coli.  Keywords: Antibiotics, E. coli, Urinary Tract Infection, Vitek, Antimicrobial Susstebility.
Evaluation of Alpha-1-acid Glycoprotein and Alpha-antitrypsin in Bronchoalveolarlavage Fluid for Extra-bronchial Tumor and Intra-bronchial Tumor in Lung Cancer Hayder Hashim Alshalah
Journal of Global Pharma Technology Volume 11 Issue 2: 2019
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Abstract

Introduction: Lung cancer is one of the most common malignant tumors and the leading cause of cancer-related death worldwide. Aim of study: to evaluate of proteins (ALpha1 antitrypsin and alpha1 acid glycoprotein) in BALF in extra –bronchial Tumor and intra- bronchial Tumor of lung cancer. Material and method: this study was carried on 40 patients with lung cancer after they have bronchoscopy.  26 patients intra  -bronchial tumor in  BALF  (this is wash area of tumor when was be inside of bronchial)   14 patients extra –bronchial tumor in  BALF(this is wash area of tumor when was be outside of bronchial ) , detection (Alpha_1_antitrypsine,Alpha 1 acid glycoprotein )  in BALF were measure by Mispa Auto-analyzer Nephelometric immunoassay method).Result we found statistical significant between groups intra &extra - bronchial tumor  to each proteins .Our study also showed that at  a cut-off  to A1AT& ,A1AGthey  has a sensitivity, specificity, positive predictive value and negative predictive value of(100 ng/ml,100%,92 %,66.6%,100%respectively with an area under the ROC of A1AT .820) ,and (AGP, 70ng/ml, 100%93.3%,%50, 100%,)  respectively with an area under the ROC of AGP .745). Conclusion: A1AT & AGP are reliable markers to diagnosis extra &intra bronchia_ tumor in BALF for lung cancer.
Osteoporosis and Risk Factors among Postmenopausal Women in Integrated Health Post for Elderly Diana Lyrawati
Journal of Global Pharma Technology Volume 11 Issue 08 (2019) Aug. 2019
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Abstract

Abstract Objectives: The availability of prevalence data for osteoporosis in Indonesia is limited due to the constrained access to BMD measurements, especially in rural areas. Integrated Health Post for the Elderly (Posyandu Lansia) is one of the Indonesian government's programs to monitor and provide health care for the elderly, including postmenopausal women. This study aimed to determine the prevalence of osteoporosis and related risk factors in postmenopausal women. Methods: Respondents were 249 postmenopausal women from 440 people who attended the Posyandu Lansia from 9 villages in Sidoarjo. Quantitative ultrasound was used for calcaneal BMD measurement; other data were collected via questionnaires and enumerated by trained villagers. Results: Our study revealed that the prevalence of osteoporosis postmenopausal women was 73.5%. The osteoporosis risk factors of age, BMI, weight, and menopause duration were associated with T-scores both individually (p<0.05) or simultaneously (p< 0.001), while menopause age and exercise did not. Conclusions: Groups possessing high-risk factors associated with a high prevalence of osteoporosis are postmenopausal women age ≥ 65 years, BMI < 20 kg/m2, weight < 58 kg and duration of menopause > 6 years. Regardless of the groupings, the prevalence of osteoporosis was very high in postmenopausal women. Dietary and exercise adjustments as well as pharmacotherapy may be required to improve their bone health. 
Correlation Study of HbA1C and Kidney Failure in Children of Iraq during Dialysis Nawal M.J. Al- Shammaa
Journal of Global Pharma Technology Volume 10 Issue 05: (2018) May2018
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Abstract

AbstractDiabetes is the major risk factor for end-stage renal disease (ESRD) worldwide. In advanced chronic kidney disease (CKD), less is known about the predictive value of HbA1c. We enrolled 2401 diabetic patients with stage 3–4 and stage 5 CKD, who were classified into 4 groups according to their baseline HbA1c values (<6%, 6%–7%, 7%–9%, and >9%). During the median follow-up of 3 years, 895 patients developed ESRD, and 530 died. In linear regression analysis, higher HbA1c correlated with higher eGFR in patients with stage 5 CKD but not in stage 3–4 CKD. In Cox regression analysis, a trend toward worse clinical outcomes existed when the HbA1c level exceeded 6% in stage 3–4 CKD, but the significance was only observed for >9%. The hazard ratios (HRs) for ESRD, all-cause mortality and combined CV events with mortality in the group of HbA1c >9% were 1.6 (95% CI, 1.07 to 2.38), 1.52 (95% CI, 0.97 to 2.38) and 1.46 (95% CI, 1.02 to 2.09), respectively. This study demonstrates that the higher HbA1c level is associated higher risks for clinical outcomes in diabetic patients with stage 3–4 CKD but not in stage 5 CKD.Diabetes mellitus (DM) is the leading cause of chronic kidney disease (CKD) worldwide, accounting for approximately 45% of end-stage renal disease (ESRD) cases in the Iraqi children dialysis population. This study was performed on 50 human females aged rang (6-12) years with on diagnosed by examining the patients by Alkademia Teaching Hospital ,also 50 males in addition to 50 healthy were enrolled in the study as control group. Measuring glycated hemoglobin (HbA1c) has been suggested as a means of assessing glycemic control in patients with diabetes, while HbA1c decrease in patients group before and after treated with mixtard. Current guidelines recommend a target HbA1c of approximately 7% for preventing or delaying microvascular complications, including diabetic kidney disease.Patients with DM and in later stages of CKD, whom were mostly excluded from clinical trials. HbA1c levels both appeared to associate with poorer clinical outcomes regardless of the baseline estimated glomerular filtration rate (eGFR). Another study showed that, in dialysis-dependent people with DM, patients with higher HbA1c levels, particularly those without anemia, exhibited poorer survival rates than did patients in the HbA1c range.To elucidate these equivocal results, we analyzed the relationships between HbA1c and the risks of ESRD and mortality in the advanced stages of diabetic CKD and tested whether different CKD stages affected these relationships. Keywords: HbA1c, DM, FBS, Albumin, creatinine, S. urea, CKD.

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