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Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 17 Documents
 1   2 
Search results for , issue "Vol 42, No 2 (2024): Agustus" : 17 Documents clear
Purifikasi dan Karakterisasi Imunoglobulin Yolk (IgY) terhadap Jembrana dari Telur Ayam sebagai Dasar Pengembangan Imunisasi Pasif Kusuma, Firdaus Lingga; Wibowo, Michael Hariyadi; Wahyuni, AETH
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.66613

Abstract

AbstrakPenyakit Jembrana adalah penyakit yang menyerang Sapi Bali (Bos javanicus) dengan penularan dan kematian yang tinggi. Penyakit ini disebabkan oleh  Jembrana Disease Virus (JDV) dari famili retroviridae. Pengendalian penyakit Jembrana di Indonesia menggunakan vaksin inaktif yang dibuat dari jaringan limfa sapi terinfeksi JDV sehingga produksinya sangat terbatas. Immunisasi pasif menggunakan antibodi terbukti mampu memberikan perlindungan terhadap berbagai infeksi. Ayam merupakan penghasil antibodi immunoglobulin yolk yang sangat baik. Tujuan penelitian ini adalah melakukan purifikasi dan karakterisasi IgY terhadap Jembrana untuk pengembangan imunisasi pasif. Sebanyak 15 ekor ayam white leghorn umur 24 minggu dibagi menjadi 3 kelompok. Kelompok 1 disuntik dengan 1ml vaksin Jembrana, kelompok 2 disuntik dengan seed virus Jembrana, dan kelompok ke 3 adalah kontrol. Penyuntikan antigen Jembrana dilakukan dua kali dengan interval dua minggu. Telur mulai dikoleksi setelah satu minggu penyuntikan antigen pertama sampai minggu ke lima. Uji antibodi Jembrana dilakukan dengan metode Agarose Gel Precipitaion Test (AGPT). Purifikasi IgY dilakukan dengan pengendapan NaCl, sedangkan untuk melihat karakter IgY Jembrana menggunakan SDS PAGE. Pengendapan NaCl mampu menghasilkan IgY dengan mudah dan biaya yang murah. Pengujian AGPT menunjukkan bahwa IgY muncul pada kelompok ayam yang disuntik dengan vaksin Jembrana, dan tidak muncul pada kelompok yang disuntik dengan seed Jembrana. Pengujian SDS PAGE menunjukkan adanya pita protein dengan berat molekul 67 kDa yang merupakan rantai berat, 46 kDa yang merupakan fragmen Fab, dan 24 kDa yang merupakan rantai ringan.
Pengaruh Pemberian Ekstrak Etanol Kulit Buah Jeruk Baby (Citrus sinensis L. Osbeck) Selama 28 hari terhadap Kadar SGOT dan SGPT Sofia, Vivi; Widyaningsih, Wahyu; Yuliani, Sapto; Bachri, Moch Saiful
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.71513

Abstract

One of the plants used as natural medicine is baby orange (Citrus sinensis L. Osbeck). Baby oranges contain hesperidin, hesperetin, narirutin and nobiletin compounds. The purpose of this study was to determine the effect of giving ethanol extract of baby orange peel for 28 days on liver function as seen from the increase in SGOT and SGPT levels. This research is a pure experimental study with a completely randomized design (CRD) with a unidirectional pattern, namely the method used to observe the relationship of independent variables (including: normal control, negative control, ethanol extract of baby orange peel (EEKBJB) doses of 50, 100, 200 and 400 mg/kg BW) with the dependent variable (SGOT and SGPT levels). The test animals used in this study were 30 male Wistar rats which were divided into 6 treatment groups which were treated for 28 consecutive days. On day 29, blood samples were taken from the orbital sinus of the eye and the levels of SGOT and SGPT were measured. There was a significant difference in the levels of SGOT and SGPT as indicated by the results of the One Way ANOVA test (p<0.05). The results of the Post Hoc-Tukey test for SGOT levels showed a significant difference between the control group and the 50 mg/kg BW dose group, while the 100, 200 and 400 mg/kg BW doses did not show a significant difference. The results of the Post Hoc-Tukey, s-HSD test for SGPT levels did not show a significant difference between the control group and the 50, 100, 200 and 400 mg/kg BW dose groups. Significant differences were shown by doses of 50 and 400 mg/kg BW. Giving EEKBJB for 28 days did not cause toxic effects on liver function, seen from the absence of an increase in SGOT and SGPT levels.
Infestasi Ektoparasit pada Pasien Kucing yang Memiliki Masalah Kulit di Klinik Hewan Lilipoet Yogyakarta Gunawan, Lily; Indarjulianto, Soedarmanto; Yanuartono, Yanuartono; Nurcahyo, R Wisnu; Prastowo, Joko
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.75941

Abstract

Ektoparasit merupakan problem yang sering diderita kucing, termasuk di Yogyakarta. Penelitian ini bertujuan untuk mengetahui prevalensi infestasi ektoparasit pada kucing dan jenisnya di wilayah Klinik Hewan Lilipoet Yogyakarta. Penelitian ini dilakukan menggunakan 173 pasien kucing penderita dermatitis. Data yang diambil adalah umur, jenis kelamin dan diagnosis berdasarkan pemeriksaan kulit. Sampel ektoparasit diambil dari kucing dengan metode combing. Ektoparasit diidentifikasi secara mikroskopik dari sampel kulit. Hasil penelitian menunjukkan 120 dari 173 (69,3%) kucing yang diperiksa ditemukan ektoparasit. Spesies ektoparasit tersebut terdiri dari: Otodectes cynotis (30%), Ctenocephalides felis felis (25,8%), Notoedres cati (25%), Lynxacarus radovskyi (15,8%), Felicola subrostratus (2,5%), dan Rhipicephalus sanguineus (0,8%). Sebanyak 16 dari 120 (13,3%) pasien kucing menderita infestasi beberapa ektoparasit. Jumlah ektoparasit yang ditemukan pada kucing umur 1-12 bulan sebanyak 86/120 (71,6%), umur12-24 bulan sebanyak 13/120 (10,8%), dan pada umur lebih dari 24 bulan sebanyak 21/120 (17,5%). Ektoparasit ditemukan pada kucing jantan sebanyak 69/120(57,5%) dan pada betina 51/120 (42,5%). Disimpulkan bahwa prevalensi infestasi ektoparasit pada kucing penderita dermatitis di klinik Lilipoet Yogyakarta adalah 69,3%. Infestasi ektoparasit banyak ditemukan pada kucing berumur dibawah 1 tahun dan lebih sering ditemukan pada kucing jantan daripada betina.
The Prevalence of Dairy Goat’s Subclinical Mastitis Related to Udder Morphology and Farming Management Practices Khasanah, Himmatul; Yulianto, Roni; Widodo, Nur; Widianingrum, Desy Cahya; Rusdiana, Riza Yuli
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.76090

Abstract

This study aimed to analyze the prevalence of subclinical mastitis (SCM) and the correlation between CMT positive score, udder morphology and management practice in dairy goats in several regencies in East Java. The SCM identification was tested using the California Mastitis Test (CMT) method. The obtained data were analyzed qualitatively and quantitatively, and the correlation was determined using Kendall’s tau_b coefficient correlation method. The results showed the prevalence of SCM in Blitar, Lumajang, and Jember amounted to 19,35%, 0,86%, and 25%, respectively. The control management of observed dairy farms was relatively similar such as the milking frequency, feeding management, health control, and cage type. Milk production was moderately correlated with CMT (tb =-0.417) and low correlation with the udder shape (tb =1.51), side udder cross-section (tb =-0,293), teat angle of separation between teat (tb =0,204), and degree of udder separation (tb =0,128). The CMT also has low correlation with the BCS (tb =0,146), udder symmetry (tb =0,126), sided udder cross-section (tb =0,153). Then CMT also has a moderate correlation with housing type (tb =-0,380) and milking frequency (tb =0.365). In conclusion, the prevalence of SCM was relatively low to moderate and its correlation with udder morphology were low to moderate. 
Study of Ovary Follicular Profile and Estrogen Concentration of Peranakan Ongole (PO) Cows at Estrous Phase in Mountains and Lowland at Kulon Progo District, Yogyakarta Fani, Rifia Tiara; Budiyanto, Agung; Kusumawati, Asmarani
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.77613

Abstract

Ovarian follicle dinamycs and estrogen concentration interrelated to cow reproduction traits. Some previous studies said that preovulatory follicle directly related to estrogen concentration which induce estrous quality, and increasing pregnancy rate when artificial insemination happened. Another important factor to optimizing reproduction performance is altitude. Here in Indonesia these factors has not been studied further in PO cows. A total of 24 Peranakan Ongole (PO) cows, 12 cows from highlands or mountains of Kalibawang, Kulon Progo, Yogyakarta, Indonesia and 12 cows from lowlands or lowland of Galur District, Kulon Progo, Yogyakarta, Indonesia selected based on criterias; these cows ever had calves, not pregnant, 3-8 years old, have a Body Condition Score (BCS) of 2.5-3.5, in the peak phase of estrus and not experienced reproductive problems. The diameter of the cow ovarian follicles were examined using ultrasound and sera were taken to calculated the estrogen concentration using ELISA, meanwhile the NSPC taken from recording and farmer interview. The result showed that no difference of follicle diameter and estrogen concentration between mountain and lowland (p>0,05), a significant difference found in NSPC between mountain and lowland (p<0,05), and a strong correlation found betweend follicle diameter, estrogen concentration and NSPC (p<0,05), proved that the larger the follicle diameter, the higher the estrogen level and will improve the reproductive trait of PO cows.
Analysis of Storage Time for Merawang Chicken Eggs (Gallus Gallus) against Hatchability: A Systematic Review Mafruchati, Maslichah; Makuwira, Jonathan
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.80970

Abstract

Background: Merawang chicken has the potential to be developed, both as local laying hens and local broilers. Hatchability and quality of hatching eggs are influenced by storage method, storage time, storage area, ambient temperature, incubator temperature, reversal during hatching. Storage that is too long causes the quality and hatchability to decrease so eggs should be stored no more than 7 days. Purpose: The purpose of this study was to analyze the length of storage of Merawang chicken eggs (Gallus gallus) on hatchability. Research methods: A literature search was carried out systematically through the PubMed, NCBI, Google Scholar databases using keywords, namely, "Storage Period, Chicken Eggs, Merawang (Gallus Gallus), Hatchability". Based on these keywords, the articles obtained were first selected by setting several inclusion criteria. Research results: Based on the search results in the PubMed, NCBI, Google Scholar databases using predetermined keywords, 15400 articles were obtained for the keywords “Storage Time of Merawang Chicken Eggs (Gallus Gallus), Hatching Power”. All articles were reselected based on inclusion criteria and exclusion and obtained as many as 23 articles that met the inclusion criteria. Conclusion: From the results it can be concluded that the storage time is 9 days (P5) showed hatchability, high fertility and lowest embryo mortality. Need research on the other animal for future research.
Corona Virus pada Anjing dan Kucing di Yogyakarta dan Sekitarnya Mulyani, Guntari Titik; Budhi, Setyo; Raharjo, Slamet
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.83658

Abstract

Corona virus adalah virus RNA, terutama menyebabkan penyakit pernapasan dan/atau enterik dan ditemukan pada banyak spesies hewan, termasuk hewan liar, hewan peliharaan, dan manusia. Covid 19 adalah corona virus pada manusia yang sangat menular dan saat ini sedang  mewabah di seluruh dunia. Tujuan penelitian ini adalah untuk mendeteksi kasus infeksi corona virus pada hewan kesayangan. Penelitian didahului dengan screening hewan yang akan dideteksi. Peneguhan diagnosis infeksi corona dilakukan dengan pemeriksaan hematologi, dan kimia darah. Sebanyak 9 pasien anjing hasil screening dengan gejala anoreksia, muntah, diare, kelemahan dipilih untuk dideteksi adanya infeksi Canine Corona Virus (CCoV) dengan Rapid test antigen CCoV. Sebanyak 10 pasien kucing dengan gejala anoreksia, kekurusan, diare, demam, ascites dipilih untuk dideteksi adanya infeksi Feline  Corona Virus (FCoV) dengan rapid test antigen dan antibodi FCoV. Deteksi terhadap infeksi Covid 19 dilakukan terhadap 9 pasien anjing (5 sehat dan 4 dengan problema respirasi) dan 10 pasien kucing (5 sehat dan 4 dengan problema respirasi) dengan gejala anoreksia, demam, batuk, pilek menggunakan rapid test antibodi IgG dan IgM dan antigen Covid 19. Hasil deteksi dijumpai 1 sampel anjing positif Ag CCoV, 9 sampel kucing positif Ab FCoV,  dan 1 sampel kucing positif Ab IgG Covid 19. Dari hasil penelitian ini dapat disimpulkan bahwa di wilayah Yogyakarta dan sekitarnya telah ditemukan kasus infeksi corona virus, baik CcoV pada anjing, FcoV pada kucing, maupun Covid 19 pada kucing. Dari hasil penelitian ini disarankan utuk lebih meningkatkan tindakan pencegahan, dan melakukan terapi dan isolasi terhadap hewan sakit.
Efek Suplementasi Tepung Tulang Ikan Bandeng (Chanos chanos) dengan Metode Freeze Dry terhadap Performa Otot Ayam Bangkok (Gallus gallus domesticus) Astuti, Pudji; Yuneldi, Rizki Fitrawan; Divani, Adelia Lintang; Zahra, Nafisa Az; Rochmah, Jayanti Astridya; Airin, Claude Mona; Sarmin, Sarmin
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.85681

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tepung tulang ikan bandeng (Chanos chanos) yang dikeringbekukan menggunakan freeze-dry terhadap kadar testosteron pada otot pectoralis, bobot otot pectoralis, dan lingkar dada pada ayam bangkok (Gallus gallus domesticus). Penelitian ini menggunakan 10 ekor ayam bangkok jantan dengan umur 3-4 bulan yang terbagi menjadi dua kelompok perlakuan yaitu P0 (kontrol) dan P1 (tepung tulang ikan bandeng sebanyak 3,3 g/ekor/hari). Sebelum dilakukan penelitian dilakukan aklimatisasi selama 7 hari dan tepung tulang bandeng yang sudah dicampurkan dengan pakan diberikan setiap hari secara oral selama 35 hari. Pengukuran lingkar dada ayam bangkok dilakukan pada hari ke 7, 14, 21, 28, dan 35. Pada akhir penelitian ayam dinekropsi, kemudian dilakukan penimbangan otot pectoralis dan sampel homogenat otot pectoralis disentrifugasi dingin. Selanjutnya supernatan dikoleksi dan dianalisis menggunakan metode Enzyme-Linked Immunosorbent Assay (ELISA) untuk mengetahui kadar testosteron pada otot pectoralis. Data yang diperoleh kemudian dianalisis menggunakan independent sample T-Test dengan bantuan SPPS versi 26. Hasil penelitian menunjukkan bahwa terdapat perbedaan yang signifikan (Sig. (2-tailed) < 0,05) pada kadar hormon testosteron otot pectoralis, bobot otot pectoralis dan lingkar dada ayam bangkok. Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian tepung tulang ikan bandeng dengan metode freeze-dry dengan dosis 3,3 g/ekor/hari secara oral selama 35 hari dapat meningkatkan kadar testosteron pada otot pectoralis, bobot otot pectoralis dan lingkar dada pada ayam bangkok.
Survei Infeksi Salmonella spp. pada Pasien Anjing dan Kucing di Klinik/Rumah Sakit Hewan Daerah Istimewa Yogyakarta Kajang, Elphan Augusta; Nugroho, Widagdo Sri; Haryanto, Aris
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.86438

Abstract

Salmonellosis is an infectious disease that is zoonotic and includes food borne disease. Salmonella spp. can also be found in pets so that it can be a source of transmission of Salmonellosis to humans. The purpose of this study was to determine the infection level of Salmonella spp. in dogs and cats in clinics/animal hospitals in the Special Region of Yogyakarta. A total of 250 rectal swabs from 83 dogs and 167 cats were taken in this study. Isolation and identification of Salmonella spp. using Xylose Lysine Deoxycholate (XLD), Triple Sugar Iron Agar (TSIA), and Lysine Iron Agar (LIA) media. The isolates were confirmed by Polymerase Chain Reaction (PCR)using a primer Forward primer (5'- GCT AAG TAT GAC ATT CCG GT -3') and reverse (5'- CCA AAG ACT ATC TGC GGA AT -3') eith targeting the STM2773 (IroB) gene. Information on the patient's medical history was obtained based on the anamnesis and questionnaire to the animal owner. Data analysis was descriptive statistics. A total of 32 isolates of Salmomella spp. obtained through conventional methods and as many as 30 samples (12%) confirmed Salmonella spp. using PCR. A total of 11 (13.25%) of 83 dogs and 19 (11.27%) of 167 cats were identified as positive for Salmonella spp. The results of this study indicated that 12% of pet animals in the Special Region of Yogyakarta were infected with Salmonella spp.
Studi Perilaku Harian Rusa Timor (Cervus timorensis) di Lembaga Konservasi PT Taman Satwa Semarang Husna, Noora Lailatul; Kasiyati, Kasiyati; Djaelani, Muhammad Anwar; Setiawan, Hendrik Tri; Puri, Nur Liliana; Alrais, Aditya
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.89306

Abstract

Adaptation of timor deer at the Conservation Institution of PT Taman Satwa Semarang is formed through a learning and memory process. The presence of visitors stimulates the deer to walk towards the side of the pen even though it is resting. Drop in feeding by keepers in the morning, afternoon, and evening causes the deer to become more active during the day. This study aims to analyze frequency and duration of the daily behavior of timor deer on weekdays with few visitors and on weekends with lots of visitors. Behaviors observed include moving, feeding, resting, grooming, aggressive, eliminative, and reproductive behavior. This research was conducted for 30 days. The object used in this study was a population of 18 adult timor deer. The method used is scan sampling, data recording of daily behavior which includes duration and frequency are carried out periodically at 10-minute intervals. The collected data includes daily behavioral, type of feed, as well as temperature and humidity. Data analysis was performed using t-test at a significance level of 5%. The results showed that visitor activity between weekdays and weekends had a significant effect (p˂0.05) on moving, feeding, resting, grooming, aggressive, and reproductive behavior. The conclusion of this study was the most timor deer perform resting and reproductive behaviors on weekdays, while moving, eating, and social behavior are mostly carried out on weekend.

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