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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 845 Documents
 81   82   83   84   85 
Ekspresi TNF-α Histopatologi Ginjal Pada Tikus Model Sepsis Sebagai Efek Preventif Diberi Nanoemulsi Burdock Yang Diinduksi Bakteri MRSA Faizal, Imam Agus; Nugroho, Yusuf Eko; Fauziah, Rahma Shifa; Yunadi, Frisca Dwi; Swandari, Mika Tri Kumala
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.107607

Abstract

One of the natural ingredients utilized as a traditional medicinal herb is Arctium lappa L. or commonly called burdock. This plant has roots, leaves, seeds that have been used to treat infections and various skin disorders. Burdock is used for anti-inflammatory, anti-cancer, infection-fighting, and anti-tumor regulating activities. The purpose of this research is to determine the effect of Arctium lappa L. root extract on renal histopathology and tumor necrosis factor-alpha (TNF-α) expression in white rat sepsis model after Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria administration. Where in this research stage is divided into 5 groups of test animal treatment. Each group was injected with MRSA bacterial suspension with a standard MC Farland turbidity test of 0.5. The results of this research obtained that burdock root extract is able to inhibit inflammation of sepsis test animals caused by MRSA bacteria with the average value of TNF- α reaction presentation, namely K1 (-) (13.632; 14.756), K2 (+) (33.291; 29,568), K3 (150 mg/kg BW) (35,458; 33,273), K4 (300 mg/kg BW) (36,120; 28,731) K5 (450 mg/kg BW) (28,086; 228,084. So it can be stated that Arctium lappa L root extract is able to inhibit inflammation with the use of the most effective dose of 450 mg/kg BW.
Kualitas Semen Cair Domba dalam Pengencer Komersial Berbasis Tris Kuning Telur dengan Penambahan Glutation Pangestu, Dyah Mekar; Prasetyaningtyas, Wahono Esthi; Fahrudi, Mokhamad; Karja, Ni Wayan Kurniani
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.109646

Abstract

Penyimpanan semen cair berpotensi meningkatkan pembentukan radikal bebas dan stres oksidatif, yang menyebabkan kerusakan membran plasma spermatozoa dan penurunan kualitas semen. Glutation merupakan antioksidan yang mampu menetralkan radikal bebas dan mengurangi kerusakan oksidatif, sehingga berpotensi menjaga kualitas spermatozoa selama penyimpanan. Penelitian ini bertujuan mengevaluasi pengaruh penambahan glutation terhadap kualitas semen cair domba dalam pengencer komersial berbasis tris kuning telur. Semen segar dengan motilitas ≥70% diencerkan dengan penambahan glutation pada konsentrasi 0 mM (GSH-0), 2 mM (GSH-2), dan 4 mM (GSH-4), kemudian disimpan pada suhu 4  selama enam hari. Evaluasi dilakukan setiap 24 jam terhadap motilitas, viabilitas, keutuhan membran plasma (MPU), dan abnormalitas spermatozoa. Hasil hari keenam menunjukkan bahwa GSH-4 memberikan hasil terbaik, dengan motilitas 64,00 ± 2,24%, viabilitas 84,95 ± 1,12%, MPU 85,93 ± 0,46%, dan abnormalitas 9,47 ± 0,66%. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa penambahan glutation 4 mM efektif dalam mempertahankan kualitas semen cair domba selama pada suhu 
Protective Effects of Red Spinach (Amaranthus tricolor L.) Ethanolic Extract on Tracheal Histopathology in Cigarette Smoke-Exposed Wistar Rats Setiawan, Haris; Agrisukma, Salsabilla Ari Sekar; Putra, Ichsan Luqmana Indra
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.111588

Abstract

Cigarette smoke generates free radicals that can cause various respiratory disorders. Red spinach (Amaranthus tricolor L.) is a plant rich in antioxidants, which may help neutralize free radicals produced by cigarette smoke exposure. This study aimed to evaluate the protective effects of red spinach ethanol extract on the trachea of Wistar rats exposed to cigarette smoke. Twenty-four male Wistar rats were randomly assigned to four groups: K (distilled water), KN (cigarette smoke exposure), P1 (cigarette smoke exposure with 200 mg/kg BW extract), and P2 (cigarette smoke exposure with 400 mg/kg BW extract). Treatments were administered daily for 21 consecutive days. On day 22, the rats were euthanized, and their tracheas were collected for histological analysis. The tracheal tissues were processed using the paraffin embedding method and stained with hematoxylin and eosin. The evaluated parameters included epithelial cell height, tracheal lumen diameter, and goblet cell number. Data were analyzed using one-way ANOVA followed by Duncan’s post hoc test, with significance set at p < 0.05. Results demonstrated that exposure to cigarette smoke (KN group) significantly disrupted the tracheal histological structure (p < 0.05). However, administration of 400 mg/kg BW red spinach ethanol extract (P2 group) exhibited a protective effect by preserving epithelial cell height, lumen diameter, and goblet cell number comparable to those of the control group (K). In conclusion, red spinach ethanol extract at a dose of 400 mg/kg BW effectively protects the tracheal structure of Wistar rats against cigarette smoke-induced damage by maintaining epithelial cell height, lumen diameter, and goblet cell number at near-normal levels.
Morphology of Erythrocytes and Leukocytes of the Lesser Sunda Islands Pit Viper (Trimeresurus insularis): The Green Coloration from Bali Island and the Blue Coloration from Komodo Island Diza Pakom, Muhammad Sofi; Wandia, I Nengah; Setiasih, Ni Luh Eka; Komala Sari, Tri; Kardena, I Made; Suartini, I Gusti Ayu Agung
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.111802

Abstract

Indonesia is a tropical country with a diverse range of herpetofauna, one of which is the Trimeresurus insularis snake, a highly venomous species endemic to Indonesia. This snake is distributed across Java, Bali, West Nusa Tenggara, East Nusa Tenggara, and Wetar Island in Maluku Province. Despite its wide distribution, this species exhibits variations in body coloration. T. insularis from Bali is green, while those from Komodo Island are blue; however, both share the same red tail. This study aims to examine the morphology and morphometry of erythrocytes and leukocytes in T. insularis from different localities and color variations. The research utilized blood smear preparations from six healthy T. insularis individuals—three green coloration from Bali and three blue coloration from Komodo Island—analyzed using a microscope and Optilab Viewer camera. Cell morphometry was measured using ImageJ software. The morphometric measurements of erythrocytes included the length and width of the cytoplasm and nucleus, while leukocyte morphometry covered the cytoplasmic and nuclear diameters of heterophils, basophils, eosinophils, azurophils, lymphocytes, and monocytes. The erythrocyte morphology of T. insularis from both Bali and Komodo Island showed no differences. Morphometric data were analyzed using the Independent-Samples T Test. The results showed significant differences in the length and width of the erythrocyte nucleus, as well as in the cytoplasmic and nuclear diameters of lymphocytes. Meanwhile, no significant differences were found in erythrocyte length and width, as well as in the cytoplasmic and nuclear diameters of heterophils, basophils, eosinophils, and monocytes.
Identification of Lactic Acid Bacteria From Pineapple Peels Fermented Beverages Through 16S rRNA Gene Analysis Ariwidiani, Ni Nyoman Nyoman; Pauzan, Pauzan; Wahdaniyah, Rabiyatun; Mahavira Diputra, I Ketut
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.112560

Abstract

Stunting is associated with intestinal microbiota dysbiosis, which disrupts bodily functions and contributes to stunted growth in children. Consuming probiotics is an alternative way to prevent gastrointestinal infections caused by dysbiotic microbiota. Probiotics work by balancing the composition of the intestinal microbiota and stimulating the immune response. The use of fermented products is well known; one example is tepache, a fermented pineapple peel drink containing lactic acid bacteria with potential as a probiotic. This study aims to identify lactic acid bacteria from fermented pineapple peel drinks with potential as probiotics for intestinal microbiota health. Pineapple peel fermentation was carried out for 3 days; the resulting fermentation liquid was cultured on MRS agar and tested for characteristics using biochemical tests. Identification of isolates from the pineapple peel fermentation culture was performed by sequencing the 16S rRNA gene. The results of the isolate culture showed growth of 2 bacterial isolates with characteristics consistent with LAB colonies. Microscopic tests showed that isolate N1 was a gram-positive, rod-shaped LAB, and isolate N2 was a gram-positive, cocobacilli-shaped LAB. Identification of the 16S rRNA gene from isolate N1 showed characteristics similar to Lactiplantibacillus plantarum, while isolate N2 showed characteristics similar to Leuconostoc mesenteroides. These isolates are common lactic acid bacteria found in fermented foods and may be potential probiotic candidates, but functional and safety evaluations are needed to confirm their probiotic properties.
Preparasi dan Karakterisasi Material Pengisi Nanokomposit Antibakteri dari Limbah Daun Nanas untuk Aplikasi Medis Ratulangi, Wulan Ratia; Widyan, Rosnalia
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.112621

Abstract

Infeksi bakteri merupakan salah satu permasalahan utama dalam penggunaan material medis seperti wound dressing, implan, dan biomaterial lainnya. Oleh karena itu, material dengan kemampuan antibakteri menjadi sangat penting untuk mencegah pertumbuhan mikroorganisme patogen yang dapat menyebabkan infeksi pada jaringan tubuh. Serat selulosa berbasis biomassa dapat berfungsi sebagai matriks atau bahan pengisi yang mendukung penambahan agen antibakteri, sehingga menghasilkan material yang tidak hanya memiliki sifat mekanik yang baik tetapi juga memiliki aktivitas biologis yang bermanfaat. Pemanfaatan nanofiber dari limbah daun nanas sebagai bahan pengisi nanokomposit antibakteri merupakan salah satu strategi yang menjanjikan dalam pengembangan biomaterial medis yang berkelanjutan. Penelitian ini bertujuan untuk melakukan preparasi dan karakterisasi nanofiber dari limbah daun nanas sebagai material pengisi nanokomposit yang berpotensi digunakan untuk aplikasi medis. Penelitian ini menggunakan true experimental design. Proses preparasi dilakukan melalui tahapan degumming menggunakan larutan NaOH 5% untuk menghilangkan komponen non-selulosa seperti lignin, hemiselulosa, pektin, dan pengotor lainnya, yang dilanjutkan dengan proses bleaching menggunakan H₂O₂ untuk meningkatkan kemurnian selulosa. Karakterisasi material dilakukan menggunakan Fourier Transform Infrared Spectroscopy (FTIR) untuk menganalisis perubahan gugus fungsi kimia, Scanning Electron Microscopy (SEM) untuk mengamati morfologi permukaan serat, serta uji mekanik (uji tarik) untuk mengevaluasi sifat mekanik serat. Hasil analisis FTIR menunjukkan penurunan intensitas peregangan gugus karbonil pada rentang 1730–1750 cm⁻¹ yang mengindikasikan berkurangnya komponen hemiselulosa dan pengotor pada serat. Hasil pengamatan SEM menunjukkan bahwa perlakuan kimia mampu mengurangi sebagian besar lapisan lignin, lilin, dan pektin pada permukaan serat sehingga menghasilkan struktur nanofiber dengan luas permukaan yang lebih tinggi. Uji mekanik menunjukkan bahwa serat daun nanas memiliki potensi sebagai bahan penguat alami dalam material komposit meskipun terjadi penurunan kekuatan tarik setelah perlakuan kimia. Secara keseluruhan, nanofiber dari limbah daun nanas memiliki karakteristik yang baik dan berpotensi dikembangkan sebagai material pengisi nanokomposit antibakteri yang ramah lingkungan untuk berbagai aplikasi medis.
Utilization of Filter Paper as an Innovative Transport Medium for Salmonella typhi and Shigella dysenteriae Bacteria: A Review of Effectiveness. Atfal, Bustanul; Hijriani, Baiq Isti; Maola, Seftia Dhina
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.112999

Abstract

The World Health Organization (WHO) released the Guidance on Regulations for the Transport of Infectious Substances 2023–2024 to minimize the risk of disease transmission caused by mishandling or leakage of infectious materials during transport. This initiative is reinforced by the Indonesian Directorate General of Civil Aviation, which restricts the amount of liquid material allowed on both domestic and international flights. In line with these policies, this study aimed to assess the effectiveness of filter paper as an alternative transport medium for Gram-negative bacteria, Salmonella typhi and Shigella dysenteriae, based on different storage durations at 37°C in the URB-Balitbangkes Laboratory in RSUP West Nusa Tenggara. The research employed an experimental laboratory design, using six samples with three repetitions, which resulted in 18 culture cups for each bacterium. Biochemical and Gram-staining tests confirmed positive results for both S. typhi and S. dysenteriae on the filter paper transport media. Bacterial growth remained consistent (+3) across all storage periods of 1, 2, 3, 4, 7, and 14 days. The findings demonstrate that filter paper can function effectively as a dry preservation medium, offering a practical and safe method for transporting infectious bacterial cultures over time.
Exploitation of Antibiofilm Substances by Endophytic Bacteria of Renggak Fruit (Amomum dealbatum) Against Staphylococcus aureus, the Cause of Nosocomial Infections Hijriani, Baiq Isti; Atfal, Bustanul; Kamila, Natasya Cahya
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.113070

Abstract

Nosocomial infections can be caused by biofilm-forming microorganisms. The ability to form biofilms results in persistent, recurrent, and 100-1000 times more resistant to antibiotics. One of the biofilm-forming bacteria is Staphylococcus aureus. The renggak plant (Amomum dealbatum Roxb.) which contains alkaloids, steroids, flavonoids, terpenoids, tannins, and saponins is known to play a role in inhibiting bacterial biofilm formation. This study used an in vitro experimental design aimed to analyzing the antibiofilm activity of endophytic bacteria against Staphylococcus aureus using the plate tissue culture method. The results obtained five endophytic bacterial isolates, including BI1, BI2, BI3, BI4, and BI5. The percentage of biofilm inhibitory activity by cell-free supernatants of endophytic bacteria was 35%, 0%, 37%, 44%, and 39%, respectively, with a positive control was 42%. Meanwhile, the biofilm revealing activity was 39%, 42%, 41%, 38%, and 36%, respectively, with a positive control was 56%. The results of the One Way ANOVA statistical test obtained a significance result of 0.000 (p<0.005), which means there was a significant difference between the treatment groups in the antibiofilm test. The conclusion is the cell-free supernatant of endophytic bacteria from renggak fruit has inhibitory and degradation activity against Staphylococcus aureus bacterial biofilms.
HUBUNGAN KARAKTERISTIK DEMOGRAFI, PENGETAHUAN, DAN SIKAP TERHADAP PRAKTIK HIGIENE SANITASI PEKERJA RUMAH POTONG HEWAN-UNGGAS DI DKI JAKARTA Whardani, Vivien Kusuma; Sudarnika, Etih Etih Sudarnika; Purnawarman, Trioso
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.114947

Abstract

Peningkatan konsumsi daging ayam sebagai pangan asal hewan menuntut penerapan higiene dan sanitasi yang baik di Rumah Potong Hewan Unggas (RPHU) untuk menjamin keamanan pangan dan kesehatan masyarakat veteriner. Penelitian ini bertujuan menganalisis hubungan pengetahuan, sikap, dan praktik higiene sanitasi pekerja RPHU di Provinsi DKI Jakarta. Penelitian dilaksanakan pada enam RPHU dengan melibatkan 90 pekerja area bersih. Data dikumpulkan melalui kuesioner pengetahuan, sikap, dan praktik serta observasi lapangan, kemudian dianalisis secara deskriptif dan menggunakan analisis jalur. Hasil penelitian menunjukkan bahwa pengetahuan dan sikap pekerja umumnya berada pada kategori sedang hingga baik, sedangkan praktik higiene sanitasi masih didominasi kategori sedang. Analisis jalur menunjukkan bahwa pendidikan berpengaruh signifikan terhadap pengetahuan, pengetahuan berpengaruh signifikan terhadap sikap, serta pengetahuan dan sikap berpengaruh signifikan terhadap praktik kebersihan sanitasi, sedangkan umur berpengaruh negatif terhadap praktik. Temuan ini menunjukkan bahwa peningkatan praktik higiene sanitasi di RPHU memerlukan penguatan pengetahuan dan sikap pekerja melalui pendidikan dan pelatihan berkelanjutan, disertai pengawasan yang konsisten dan dukungan sarana sanitasi yang memadai guna menurunkan risiko kontaminasi dan meningkatkan keamanan pangan asal hewan. 
Perbandingan Perilaku Harian Sapi Potong pada Alas Kandang yang Berbeda Kasiyati, Kasiyati; Muhammad, Fuad; Hidayat, Jafron Wasiq; Rahadian, Rully; Maryono, Maryono
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.115742

Abstract

Using appropriate bedding can enhance the welfare of beef cattle. This study was designed to evaluate the daily behavior of beef cattle kept on different types of bedding. Field research was conducted over a period of two months, using eight beef cattle, which were divided into two groups based on the type of bedding used, namely straw and rubber mats. The daily behaviors observed were the duration and frequency of eating, aggressive behavior, lying down, sleeping, standing, and elimination. The observation used was an intermittent method. The first group consisted of a pen block with straw bedding, and the second group consisted of a pen block with carpet bedding. Data analysis used the t-test and analysis of variance at a significance level of 5%. The results showed that all parameters did not reveal any differences; however, sleeping behavior was higher (P < 0.05) in the straw-bedded pens, with the highest percentage of sleep occurring in the afternoon. This study concluded that both straw and rubber carpet bedding are still capable of supporting the normal expression of the main daily behaviors of cows. Both types of bedding are still suitable for use in beef cattle husbandry systems.

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