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Journal of Medical Laboratory Research
Published by Politeknik Kesehatan Kementerian Kesehatan Banten
ISSN : -     EISSN : 29642892     DOI : 10.36743
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology
Journal of Medical Laboratory Research is a scientific journal published by Politeknik Kesehatan Kementerian Kesehatan Banten. The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 43 Documents
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Identification Of Candida albicans in Oral Mucosal Swabs Of Smokers In The Gang Siswa Raya Kampung Sirnagalih Kota Tangerang Fadillah, Muhammad Arief; Ratu Muawwadah; Ridwanulloh, Mohammad; Hamtini, Hamtini; Nurnida, Rossy
Journal of Medical Laboratory Research Vol. 3 No. 1 (2024): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Abstract

Candidiasis is a primary or secondary mycotic infection caused by members of the genus Candida and other genera, where Candida is one of the various forms of yeast. This study aims to determine the presence of Candida albicans fungus in oral mucosa swabs of smokers in the Gang Siswa Raya, Kampung Sirnagalih, Kota Tangerang based on smoking frequency and duration of smoking. This study uses a descriptive method, namely research with the aim of describing the presence of Candida albicans in oral mucosa swabs of smokers in the Gang Siswa Raya, Kampung Sirnagalih, Kota Tangerang using Sabouraud Dextrose Agar media and Germ tube tests. A total of 20 samples from 97 smokers data were collected. The results of the examination showed that there were fungi in the oral mucosa swab samples of smokers in the Gang Siswa Raya, Kampung Sirnagalih, Kota Tangerang as much as 40%. Then there is the growth of Candida albicans fungus in smokers with a smoking frequency of >3 years as much as 40%. Then there is the growth of Candida albicans fungus in smokers with a smoking frequency of >10 cigarettes a day as much as 35%.
Perbedaan Varian Waktu Puasa Terhadap Nilai Glukosa Darah Puasa 8 Jam, 10 Jam dan 12 Jam Pada Pasien Glaukoma Di RSU Bunda Jembrana Oki wibawa putra oki; Anak Agung Eka Cahyani; Diah Prihatiningsih
Journal of Medical Laboratory Research Vol. 4 No. 1 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Abstract

Blood glucose level is a term that refers to the level of glucose in the blood. Blood glucose levels increase after meals and are at their lowest in the morning before meals. This study aimed to determine differences in fasting time variations in 8-hour, 10-hour, and 12-hour fasting blood glucose values in glaucoma patients at Bunda Jembrana General Hospital. This study is a quasi-experimental study. The population used in this study was all glaucoma patients at Bunda Negara General Hospital. The sampling technique used in this study was probability sampling using proportionate stratified random sampling. Data collection in this study was conducted through primary data collection. The data were obtained from fasting blood glucose tests on 40 respondents, selected from glaucoma patients. Each respondent underwent three fasting sessions: 8 hours, 10 hours, and 12 hours, resulting in 120 data points. The examination was conducted using a spectrophotometer. The data analysis technique used in this study is the ratio data scale. The data were normally distributed with the one sample Kolmogorov-Smirnov test. The results of the study showed that the average 8-hour blood glucose level of glaucoma patients was 171.8 mg/dl, the average 10-hour blood glucose level of glaucoma patients was 165.1 mg/dl, and the average 12-hour blood glucose level of glaucoma patients was 160.1 mg/dl with a difference in the average 8-hour, 10-hour and 12-hour blood glucose levels of glaucoma patients of 7.8 mg/dl (19.5%). The SPSS Anova Multiple Comparisons test showed that there was a significant difference between the fasting blood glucose levels of 8 hours, 10 hours and 12 hours in glaucoma patients.
Perbedaan Kadar Bilirubin Total Dengan Paparan Cahaya Yang Segera Diperiksa dan Ditunda Selama 3 jam Made Gita Candra Dewi; Ni Luh Putu Devhy; Putu Ayu Parwati
Journal of Medical Laboratory Research Vol. 4 No. 1 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Abstract

Total bilirubin examination serves to determine liver and bile duct function. Pathological hyperbilirubinemia can cause central nervous system disorders to death. Due to the importance of bilirubin results for supporting diagnosis, examinations must follow procedures. However, in the field, there are often issues like lack of control, damaged equipment, and others, resulting in delayed specimens. This study aims to determine the difference in total bilirubin levels with light exposure examined immediately and delayed for 3 hours. This experimental research used 36 sample units from 18 samples with 2 treatment groups: serum exposed to light examined immediately and serum exposed to light delayed for 3 hours. Sampling technique: accidental sampling. Data analysis used the Wilcoxon test. The results showed an average total bilirubin level of 3.1444 mg/dL for immediate examination and 3.1044 mg/dL for delayed examination. The data analysis result p-value 0.000, indicates a significant difference in total bilirubin levels between immediate and delayed examination. This difference occurs due to external and internal factors. External factors include light exposure, storage time, and inappropriate storage temperature. The blue light in lamps can affect the results, leading to a decrease in total bilirubin levels.
Perbedaan Kadar Asam Urat Yang Diperiksa Segera Dengan Disimpan 24 Jam Pada Suhu Ruang Devita Kumala Dewi; Anak Agung Eka Cahyani; Sri Idayani
Journal of Medical Laboratory Research Vol. 4 No. 1 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36743/jomlr.v3i2.911

Abstract

Laboratory examination plays a crucial role in establishing the diagnosis of disease. However, uric acid samples may undergo a decrease in concentration during storage due to alterations in protein levels. Such changes can be detected through laboratory testing and may gradually reduce the proportion of protein over the course of storage. Serum storage in the laboratory may be performed at room temperature. To maintain sample stability, storage is recommended at 20°C–25°C for up to five days.This study aimed to evaluate the difference in uric acid levels between serum analyzed immediately after collection and serum stored for 24 hours. An experimental design was employed, involving a total of 19 samples. Serum was examined both immediately after collection and following 24-hour storage at room temperature. Primary data were analyzed using descriptive and statistical methods, including data distribution tests and the Paired T-Test. The findings indicated that there was no significant difference in the mean uric acid levels between samples analyzed immediately and those stored for 24 hours at room temperature.It is therefore essential that prior to conducting any examination, careful attention is given to specimen handling and that all procedures strictly adhere to standard operating protocols (SOPs). This ensures reliable results and prevents potential adverse consequences for patients in the future.
Perbedaan Kadar Kolestrol Total Pada Pengonsumsi Kopi dan Bukan Pengonsumsi Kopi Di Banjar Juwet Abiansemal Ni Kadek ary Astuti; Putu Ayu Parwati; Anak Agung Ayu Eka Cahyani
Journal of Medical Laboratory Research Vol. 4 No. 1 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Abstract

Coffee contains bioactive compounds such as caffeine, cafestol, and kahweol that can influene lipid metabolism. Consumption of unfiltered coffee (boiled coffee) has the potential to increase cholesterol levels. This study aimed to determine the difference in total cholesterol levels between coffee consumers in Banjar Juwr, Abiansemal. The research employed a quantitative comparative method with an observational design. A total of 70 male respondents age 20-50 years (35 coffee consumers and 35 non-consumers) were selected using purposive sampling. Total cholesterol levels were measured using the test strip method (Easy Touch GCU). Data were analyzed using the Independent Sample T-Test. The mean total cholesterol levels of coffee consumers was 170.49 mg/dL, while that of non-consumers was 178.80 mg/dL. Statistical analysis showed p=0.103 (p>0.05), indicating no significant difference between the two groups. Conclusion Black coffee consumption in Banjar Juwet was not proven to significantly affect total cholesterol levels. Other factors such as dietary patterns, phhysical activity, and health history are presumed to play a greater role.
Potensi Antibakteri Kombinasi Ekstrak Daun Mangga (Mangifera indica L.) dan Daun Kirinyuh (Chromolaena odorata L.) Terhadap Staphylococcus aureus Syarah Anliza; Deisya Tyas Marwin; Nurmeily Rachmawati; Hamtini
Journal of Medical Laboratory Research Vol. 4 No. 1 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Abstract

Infectious diseases caused by Staphylococcus aureus are one of the health problems occurring in Indonesia. Infectious diseases can be treated with antibiotics. However, antibiotics used without proper prescription can trigger antibiotic resistance. To overcome antibiotic resistance, a combination of natural ingredients such as kirinyuh leaves (Chromolaena odorata L.) and mango leaves (Mangifera indica L.) can be used. This study aims to determine the inhibitory activity and effective concentration of the combination of mango leaves extract (Mangifera indica L.) and kirinyuh leaves  extract (Chromolaena odorata L.) as antibacterial agents against Staphylococcus aureus. The methods used in this study included maceration to prepare mango leaves and kirinyuh leaves extract, phytochemical screening to identify the presence of secondary metabolites in the kirinyuh leaves and mango leaves extract, and the disk diffusion method for antibacterial testing. The results showed inhibitory activity at all concentrations due to the presence of secondary metabolites in the kirinyuh leaves and mango leaves extracts. At a 1:3 concentration, an inhibition zone of 18 mm was obtained; at a 1:1 concentration, an inhibition zone of 19 mm was obtained; at a 3:1 concentration, an inhibition zone of 20 mm was obtained. The effective concentration of the combination as an antibacterial agent is the 3:1 concentration. The results of this study fall into the strong category, indicating that the combination of mango leaves and kirinyuh leaves extracts has antibacterial efficacy against Staphylococcus aureus.
Pengaruh Durasi Radiasi UVC Terhadap Nilai Total Plate Count (TPC) CILOK (Effect of UVC Radiation Duration on The Total Plate Count (TPC) of CILOK) Wulan Fitriani Safari; Dania Febriani Fariska; Mohamad Syafaat
Journal of Medical Laboratory Research Vol. 4 No. 1 (2025): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Abstract

Cilok is a popular street food enjoyed by various groups, but it carries a high risk of microbial contamination. One of the parameters used to assess food safety is the Total Plate Count (TPC), with a maximum limit of 1 × 10⁵ CFU/g according to BPOM standards. UVC irradiation can be applied to reduce microbial contamination in cilok. The effectiveness of UVC radiation in inactivating bacteria is influenced by several factors, such as wavelength, distance from the light source, and exposure duration. This study aims to determine whether the duration of UVC radiation affects the TPC value of cilok. Cilok samples were collected from street vendors around Binawan University and exposed to UVC radiation for 0 minutes (control), 30 minutes, and 60 minutes. TPC testing was conducted using the Pour Plate method. The results showed that UVC radiation treatment reduced the TPC value of cilok. The highest TPC value was found in the untreated sample (1.8 × 10⁶ CFU/g), followed by samples exposed for 30 minutes (4.5 × 10⁵ CFU/g) and 60 minutes (2.1 × 10⁴ CFU/g). One-way ANOVA analysis indicated a significant difference among treatment groups (p < 0.05), suggesting that UVC radiation duration has a significant effect in reducing microbial counts in cilok.
Potensi Antibakteri Fungi Endofit Kulit Batang Langsat (Lansium domesticum corr) Terhadap Bakteri Escherichia coli Merliya Dwiyanti; Hamtini Hamtini; Venny Particia; Mohammad Ridwanulloh; Ira Nuraeni; Syalma Wijatama Putri; Juli Rahmawati
Journal of Medical Laboratory Research Vol. 4 No. 2 (2026): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Abstract

Diare merupakan masalah Kesehatan yang disebabkan oleh mikroorganisme patogen termasuk Escherichia coli. Pengobatan bakteri E.coli dengan antibiotik dalam jangka panjang dapat terjadi resistensi. Oleh karena itu, diperlukan sumber antibakteri alami sebagai alternatif pengobatan. Salah satunya adalah pemanfaatan fungi endofit. Fungi endofit merupakan organisme multiseluler yang dapat menghasilkan senyawa metabolit dengan efek serupa pada tanaman inangnya. Tanaman obat yang bisa dijadikan antibiotik diantaranya adalah kulit batang langsat (Lansium domesticum) yang memiliki senyawa alkaloid, flavonoid, saponin, tanin dan triterpenoid. Senyawa ini yang memiliki metabolit sekunder yang berfungsi sebagai senyawa antibakteri. Tujuan penelitian untuk mengetahui ada atau tidaknya fungi endofit pada kulit batang langsat serta mengetahui potensinya sebagai antibakteri terhadap bakteri E.coli. metode yang digunakan pada penelitian ini adalah isolasi dan pemurnian fungi endofit, penyiapan bakteri uji E.coli, dan uji antibakteri dengan metode difusi cakram (Kirby- bauer). Hasil menunjukan bahwa terdapat 3 isolat fungi endofit dari kulit batang langsat dengan kode KB 1, KB 2, dan KB 3 yang menghasilkan spesies fungi Mucor sp. Memiliki kemampuan menghambat pertumbuhan bakteri E.coli termasuk kedalam kategori kuat dengan rerata zona hambat masing- masing 15 mm, 12 mm, dan 13 mm. Kesimpulan dari penelitian ini adalah isolat fungi endofit hasil isolasi dapat berpotensi sebagai antibakteri.
Distribusi Ekologis dan Profil Kepekaan Antibiotik Vibrio spp. pada Zona Wisata dan Budidaya di Pantai Anom, Tangerang Nurdianti Nurdianti; Maya Sari; M. Arief Fadillah; Wiwien Mukti Andriyani; Aminah Aminah
Journal of Medical Laboratory Research Vol. 4 No. 2 (2026): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Abstract

Perairan pesisir tropis berfungsi sebagai reservoir alami bakteri oportunistik, termasuk Vibrio spp., yang berpotensi memengaruhi kesehatan manusia dan keberlanjutan akuakultur. Penelitian ini bertujuan mengevaluasi distribusi ekologis dan profil kepekaan antibiotik Vibrio spp. pada zona wisata dan budidaya di Pantai Anom, Kabupaten Tangerang. Desain penelitian deskriptif eksploratif dilakukan dengan pengambilan sampel air dan sedimen dari empat lokasi representatif: air laut, sedimen pantai, air tambak, dan sedimen tambak. Isolasi dilakukan melalui pengayaan dalam Alkaline Peptone Water dan kultur pada media Thiosulfate Citrate Bile Sucrose, dilanjutkan dengan identifikasi morfologi, pewarnaan Gram, uji hemolisis, dan uji biokimia konvensional. Uji kepekaan antibiotik dilakukan menggunakan metode difusi cakram terhadap siprofloksasin (5 µg), sulfametoksazol-trimetoprim (1,25/23,75 µg), dan kloramfenikol (30 µg) sesuai pedoman CLSI. Lima isolat dengan karakteristik menyerupai Vibrio fischeri, Vibrio ordalii, dan Vibrio natriegens teridentifikasi, dan seluruhnya menunjukkan kategori sensitif terhadap antibiotik uji. Hasil ini menyediakan data dasar surveilans lingkungan dalam kerangka One Health.
Eksplorasi Limbah Organik Sebagai Media Alternatif Pertumbuhan Candida albicans: Studi Komparatif Tepung Ampas Kelapa, Ampas Tebu, Dan Nasi Aking Maya Sari; Yulian Dini; Putri Nabila Ramadanti; Galih Surya Laksono
Journal of Medical Laboratory Research Vol. 4 No. 2 (2026): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Abstract

Candida albicans merupakan fungi oportunistik yang sering menyebabkan infeksi pada individu dengan sistem imun lemah. Media Potato Dextrose Agar (PDA) umum digunakan untuk kultur fungi, namun memiliki keterbatasan dari segi biaya dan ketersediaan. Penelitian ini bertujuan untuk mengeksplorasi potensi limbah organik berupa tepung ampas kelapa, ampas tebu, dan nasi aking sebagai media alternatif pertumbuhan C. albicans serta menentukan konsentrasi optimal masing-masing media. Penelitian ini menggunakan desain eksperimental laboratorik dengan variasi konsentrasi media 0,5%, 1%, 1,5%, dan non-gula, serta PDA sebagai kontrol. Inokulasi dilakukan dengan metode spread plate dan inkubasi selama 5 hari pada suhu 37°C. Pengamatan dilakukan secara makroskopis, mikroskopis menggunakan pewarnaan Lactophenol Cotton Blue (LPCB), uji germ tube, dan perhitungan jumlah koloni (CFU/mL). Hasil penelitian menunjukkan bahwa semua media alternatif mampu mendukung pertumbuhan C. albicans dengan karakteristik morfologi yang serupa dengan media PDA. Pertumbuhan koloni pada media alternatif umumnya mulai terlihat pada hari ke-2. Konsentrasi optimal diperoleh pada tepung ampas kelapa 0,5% (44 × 10⁻⁶ CFU/mL), ampas tebu 1% (41 × 10⁶ CFU/mL), dan nasi aking 1,5% (71 × 10⁻⁶ CFU/mL). Media tanpa penambahan gula menunjukkan pertumbuhan terendah. Eksplorasi ketiga media alternatif menunjukkan bahwa limbah organik dapat berpotensi sebagai media alternatif yang efektif, ekonomis, dan ramah lingkungan untuk pertumbuhan Candida albicans, sehingga dapat menjadi pengganti media komersial dalam kegiatan laboratorium.

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