cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Bagus Muhammad Ihsan
Contact Email
ihsanfillah24@gmail.com
Phone
+6285659274496
Journal Mail Official
ihsanfillah24@gmail.com
Editorial Address
Jl. Lapan, Siantan Hulu, Kec. Pontianak Utara, Kota Pontianak, Kalimantan Barat 78242-Kalimantan Barat-Kampus A Poltekkes Kemenkes Pontianak
Location
Kota pontianak,
Kalimantan barat
INDONESIA
Jurnal Laboratorium Khatulistiwa
Published by Poltekkes Kemenkes Pontianak
ISSN : 25979523     EISSN : 25979531     DOI : https://doi.org/10.30602/jlk
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of Medical Laboratory. Jurnal Laboratorium Khatulistiwa particularly focuses on the main problems in the development of the Medical Laboratory health areas as follows: Toxicology Immunoserology Bacteriology Clinical Chemistry Parasitologi Micology And other related disciplines.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Imunologi dan Mikrobiologi (Lain-Lain)
Articles 8 Documents
 1 
Search results for , issue "Vol 6, No 1 (2022): November 2022" : 8 Documents clear
Efek Ekstrak Metanol Daun Pucuk Merah Terhadap Kadar Glukosa Darah Syari, Jajar Pramata
Jurnal Laboratorium Khatulistiwa Vol 6, No 1 (2022): November 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v6i1.1109

Abstract

Blood glucose level is the amount of glucose in the blood. High blood glucose levels can be lowered with traditional plants such as red shoots. The leaves of the red shoots are commonly used as herbal medicine as antibacterial, antioxidant and antidiabetic. The content of flavonoid compounds, alkaloids, saponins, tannins, polyphenols found in red shoots is known to have antidiabetic activity. The purpose of this study was to explain the effect of giving red shoots leaf extract on blood glucose levels. The method used is the enzymatic method with a glucometer. This research methodology is a Quasi Experimental Design. The population and samples in this study were male (Mus musculus) mice, 2-3 months old and weighing 20-30 grams with 3 treatments, namely 200 mg/kgBW, 400 mg/kgBW, 600 mg/kgBW. The results of the Simple Linear Regression test for a dose of 200 mg/kgBW, a dose of 400 mg/kgBW and a dose of 600 mg/kgBW obtained p value = 0.000 < 0.05 then Ha is accepted, which means that there is a significant effect between red shoot leaf extract on levels of blood glucose.
Peningkatan Mutu Laboratorium dalam Aspek Keselamatan dan Kesehatan Kerja pada Laboratorium Anggarsari, Desye Novita; Ni'mah, Anisa Luthfiyyatin; Malichatin, Hanik
Jurnal Laboratorium Khatulistiwa Vol 6, No 1 (2022): November 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v6i1.981

Abstract

 The laboratory is one of the academic facilities contained in an educational institution. The benefits of the laboratory being used in scientific activities and searching for valid data related to the type of practicum-based learning. In educational institutions there are several types of laboratories such as laboratories (IPA, Engineering, Computers), in the current era laboratories can develop rapidly towards science and technology. In the management of the laboratory premises there are provisions and recommendations that are applied. One of the applications in the laboratory is to provide safety & health directions. Health in the health laboratory related to each individual is related to the practicum, because health problems caused by chemicals result in disruption of productivity levels. Laboratory work safety is the use of personal protective equipment available in the laboratory to protect individuals from the dangers of practices carried out in the laboratory.
Pengaruh Lama Perendaman Arang Aktif Cangkang Kelapa Sawit Terhadap Kadar Cod (Chemical Oxygen Demand) Pada Limbah Cair Pabrik Kelapa Sawit Ratnawati, Gervacia Jenny; Triana, Linda; Lia, Sestriana; Salim, Maulidiyah
Jurnal Laboratorium Khatulistiwa Vol 6, No 1 (2022): November 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v6i1.1125

Abstract

Limbah cair dari industri kelapa sawit mengandung Chemical Oxygen Demand (COD) yang tinggi, sehingga apabila dibuang ke lingkungan akan memberikan dampak negatif, yaitu berkurangnya kadar oksigen terlarut dalam air. Pengolahan limbah cair kelapa sawit saat ini hanya menggunakan sistem kolam terbuka, akan tetapi sistem kolam terbuka memiliki kekurangan karena memerlukan lahan yang sangat luas untuk pembuatan kolam limbah dan memerlukan waktu yang lama untuk proses pengolahannya, sehingga diperlukan suatu metode yang efektif untuk pengolahan limbah tersebut, salah satunya adalah metode adsorpsi menggunakan arang aktif cangkang kelapa sawit.  Desain penelitian ini berbentuk Pre- experimental design dengan metode close refluks secara spektrofotometri. Hasil penelitian diperoleh pada 100 ml limbah cair pabrik kelapa sawit yang dikontakkan dengan arang aktif cangkang kelapa sawit dengan dosis adsorben 2 gram selama 40, 80, 120, 160 dan 200 menit didapatkan nilai persentase penurunan kadar COD sebesar 40,30%, 49,08%, 46,57%, 13,78% dan 5,84%. Berdasarkan hasil uji Regresi Lininer Sederhana diperoleh nilai signifikan p = 0,002 (p < 0,05) sehingga Ha diterima yang artinya ada pengaruh lama perendaman arang aktif cangkang kelapa sawit terhadap kadar COD (Chemical Oxygen Demand) pada limbah cair pabrik kelapa sawit.
Identifikasi Bakteri Mirip Coliform pada Media Cromocoult Coliform Agar (CCA) Evriarti, Paulina Rosa
Jurnal Laboratorium Khatulistiwa Vol 6, No 1 (2022): November 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v6i1.1047

Abstract

Coliform-like bacteria can be found when examining coliforms in fresh water using the CCA media on membrane filter method. Therefore, it is necessary to characterize and identify coliform-like colonies that grow on CCA medium so that is easier to perform colony count analysis. One hundred ml of fresh water samples were filtered using filter membrane paper and then planted in CCA medium. Two different colonies growing on the media (red (A) and pink-purple (B)) were subjected to oxidase test, gram staining and re-cultured in various growth media such as blood agar, nutrient agar, MacConkey, and B. coli agar. Both colony types were also identified with Vitek MS to determine the species. The results of the examination showed that the red colonies (code A) were coliform-like bacteria, while the pink-purple colonies (code B) were coliform bacteria. The characteristics of coliform-like bacteria are that they produce hemolysis on blood agar and do not ferment lactose. Identification with Vitek MS showed that the isolated coliform-like bacteria was Aeromonas hydrophilla. Therefore, it can be concluded that Aeromonas sp. are coliform-like bacteria that grow on CCA, so further verification is needed when counting membrane filter colonies using CCA media.
Uji Daya Hambat Air Perasan Jeruk Lemon (Citrus Limon) Terhadap Pertumbuhan Bakteri Salmonella Typhi Sutriswanto, Sutriswanto
Jurnal Laboratorium Khatulistiwa Vol 6, No 1 (2022): November 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v6i1.1126

Abstract

Salmonelosis merupakan suatu penyakit yang disebabkan karena adanya infeksi bakteri salmonella. Salmonella masuk ke tubuh melalui jalan oral, biasanya karena mengonsumsi makanan atau minuman yang sudah terkontaminasi bakteri ini. Mengeliminasi atau mengurangi jumlah patogen, terutama salmonella pada produk daging adalah dengan cara memberikan antimikrobial yang diduga dapat membunuh atau mengurangi bakteri pada daging atau ayam potong. Salah satunya adalah dengan cara “mencuci” daging tersebut dengan asam organik seperti asam sitrat yang banyak terkandung dalam lemon. Penelitian ini memiliki tujuan untuk menganalisis pengaruh air perasan jeruk lemon dalam menghambat pertumbuhan bakteri Salmonella typhi menggunakan metode difusi.  Desain penelitian ini adalah Eksperimental Semu. Penelitian ini dilakukan dengan membuat konsentrasi air perasan lemon menjadi 65%,  70%, 75%, 80%, 85%, dan 90% kemudian cakram kosong direndam pada air perasan lemon dan diaplikasikan pada media Mueller-Hinton Agar yang sudah diinokulasi dengan bakteri Salmonella typhi. Hasil penelitian dari 6 konsentrasi dengan 4 kali pengulangan didapatkan bahwa pada konsentrasi 65% didapatkan rerata zona hambat sebesar 18.6 mm, 70% sebesar 20.1 mm,  75% sebesar 21.4 mm,  80%  sebesar 23.1 mm,  85% sebesar 24.7 mm, 90% sebesar 26.5 mm. Simpulan penelitian ini adanya pengaruh dari perbedaan konsentrasi air perasan lemon 65%, 70%, 75%, 80%, 85%, dan 90% terhadap pertumbuhan bakteri Salmonella typhi dengan nilai uji ANOVA didapatkan p sebesar 0.0000 atau p < 0.05 (alpha).
Perbandingan Hasil Pemeriksaan Leukosit Pada Sedimen Urin Yang Disentrifugasi Lima Menit Dengan Variasi Waktu Tiga Menit, Tujuh Menit Dan Sepuluh Menit arfaizah, hesti
Jurnal Laboratorium Khatulistiwa Vol 6, No 1 (2022): November 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v6i1.1048

Abstract

AbstractVariations in centrifugation time have an affect on the results of urine sediment examination. Urine sediment checks are listed in regular or routine inspections. Sediment examination is very good using morning urine because it is more concentrated and the material has not been damaged or lysis. The purpose of this study was to determine the comparison of the results of the examination of urinary leukocyte sediments that were centrifuged five minute with time variations of three minutes, seven minutes and ten minutes. The research was conducted at the Clinical Pathology Laboratory of the Faculty of Health Sciences and Technology, Binawan University. The material used morning urine. The study sample was given a 2x 60 ml urine container consisting of 2 adolescents and 4 elderly people who were willing to be respondents for urine sediment examination. The result of leukocyte analysis data are known that the average number of leukocyte cells at a time of 5 minutes is 13.14 leukocyte cells / LPB, a centrifugation time of 3 minutes is known to average 9.06 leukocyte /LPB, a centrifugation time of 7 minutes the average leukocyte cell is increasing to 17.33 leukocyte cells / LPB. The results of the urinary sediment leukocytes of the Paired T-Test can be concluded that there are significant differences in the examination of urinary leukocyte sediments with variations in centrifugation time of 3 minutes, 7 minutes and 10 minutes compared to the control time 0f 5 minutes. Keywords: Leukocytes Sediments; Time Centrifuges.
Analysis Of Factors Affecting Internal Quality Assurance In Blood Glucose Examination In The Laboratory Of Budhi Asih Hospital Maji, Anisa Sekar
Jurnal Laboratorium Khatulistiwa Vol 6, No 1 (2022): November 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v6i1.1064

Abstract

Internal quality assurance activities are divided into three stages, namely the pre – analytic stage, the analytic stage, and the post – analytic stage. Examination of blood glucose requires internal quality assurance. There are several potentials that affect this implementation, such as the discovery of lysis sample. The suitability of taking blood according to the Standar Operating Procedure (SOP) must be considered. The instruments used must be in good condition, pay attention to tool mantenance and carry out Quality Control (QC) to ensure the quality of precision and accuracy in the results to be issued. Observational research design with cross sectional design involving 23 Medical Laboratory Technology (MLT) officers as respondents and 38 blood glucose samples to be examined using a chemistry analyzer. Data from quentionaires and observations were analyzed by lambda correlation test with sig <0.05. Research on internal quality assurance on the criteria of human resource the results obtained 96.9%, the SOP criteria the results obtained 91.6%, the sample quality criteria the results obtained 90.3%, and the QC criteria the results obtained 100%. The results of the lambda correlation test on four variables showed a correaltion, with the results of HR 0.008, SOP 0.016, sample quality 0.009, QC 0.016. all of the criteria for internal quality assurance are in the good category, and all of them show that there is a relation with internal quality assurance.
Aktifitas Perasan Daun Pucuk Merah (Syzygium oleana) Sebagai Bahan Alami Hand Sanitizer Dj, Herlinda Djohan; Sugito, Sugito; Slamet, Slamet
Jurnal Laboratorium Khatulistiwa Vol 6, No 1 (2022): November 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v6i1.1095

Abstract

Indonesia is a country that has a fairly high biodiversity, one of which is red shoots (Syzygium oleana). Red shoots are reported to have strong potential to inhibit bacterial growth, including tannins, saponins, flavonoids, and essential oils. To increase effectiveness in utilizing the potential of red shoots, it is made in a practical and easy-to-use dosage form. One of the effective dosage forms for topical therapy is hand sanitizer. The increasing prevalence of the corona virus has caused a shortage of hand sanitizers. Until now, there is no treatment that can cure Corona virus infection. Therefore, the World Health Organization (WHO) appeals to take preventive measures, one of which is washing hands properly. This  study aimed  to examine the antibacterial potential of red shoots (Syzygium oleana) against the growth of Staphylococcus aureus bacteria using the disc method. The juice is made in the form of a Hand Sanitizer Spay. The antibacterial activity was obtained by measuring the diameter of the inhibition zone after giving 4 variations of the concentration of the juice sample. Positive control used amoxicillin 30 g/disk and negative control used aquadest. The next stage of analysis of the quality of hand sanitizers includes organoleptic tests, pH measurements. The research design used is a quasi-experimental method. This research was started in May-December 2022. Samples in the form of red shoots were made in the form of Hand Sanitizer Spay with various concentrations of 5%, 10%, 15%, and 20% and the average inhibition zone value was 11.8 mm, 12.6 mm, 13.75 mm and 14.4 mm. Based on the results of research and computerized data processing with the Simple Linear Regression test, the value of p = 0.000 (p <0.05) means that there is an effect of the concentration of hand sanitizer squeezed from red shoots on the inhibition of Staphylococcus aureus bacteria.

Page 1 of 1 | Total Record : 8

 1 

Filter by Year

2022 2022


Reset
Filter By Issues
All Issue Vol 9, No 1 (2025): November 2025 Vol 8, No 2 (2025): Mei 2025 Vol 8, No 1 (2024): November 2024 Vol 7, No 2 (2024): Mei 2024 Vol 7, No 1 (2023): NOVEMBER 2023 Vol 6, No 2 (2023): MEI 2023 Vol 6, No 1 (2022): November 2022 Vol 5, No 2 (2022): Mei 2022 Vol 5, No 1 (2021): November 2021 Vol 4, No 2 (2021): Mei 2021 Vol 4, No 1 (2020): November 2020 Vol 3, No 2 (2020): Mei 2020 Vol 3, No 1 (2019): November 2019 Vol 2, No 2 (2019): Mei 2019 Vol 2, No 1 (2018): November 2018 Vol 1, No 2 (2018): Mei 2018 Vol 1, No 1 (2017): November 2017 More Issue