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Life Science and Biotechnology
Published by Universitas Jember
ISSN : -     EISSN : 29884713     DOI : https://doi.org/10.19184/lsb
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Life Science and Biotechnology publishing rigorously peer-reviewed research that seeks to advance our understanding of fundamental processes in biology. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, and the public worldwide. Our journal publishes peer-reviewed research of broad interest in all areas of biology and at the interface of related disciplines (Genetics, Biodiversity, Biometry, Bioinformatics, Evolution, Ecology, Physiology, Biotechnology, Developmental biology, and Biomedical science).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 5 Documents
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Search results for , issue "Vol. 2 No. 2 (2024): November 2024" : 5 Documents clear
Blood Cell Profiles and Metamorphosis of Rice Field Frog (Fejervarya cancrivora) after Heavy Metal Copper (II) Sulfate Exposure Haryanto, Haryanto; Fitri, Fadillya Maulidilla; Nurhayati, Sari; Fadilla, Aghniya Nur Rizka
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.52293

Abstract

Tadpoles represent a critical stage in frog metamorphosis and are particularly susceptible to environmental stressors. Heavy metals, such as copper, are common industrial pollutants that adversely affect aquatic ecosystems. This study aimed to evaluate the effects of copper (II) sulfate on the blood cell profiles and metamorphic development of rice field frog (Fejervarya cancrivora) tadpoles. Experimental treatments included a positive control (mineral water), a negative control (Propylthiouracil / PTU 0.1 gL-1), and exposure to heavy metal of copper (II) sulfate 0.01 mgL-1 (Cu (II)). Each treatment group comprised nine tadpoles, maintained for 14 days under controlled conditions. After 14 days, the survival rate of tadpoles exposed to Cu (II) was 66.67%. Analysis of variance (ANOVA) revealed that Cu (II) exposure significantly impacted blood cell profiles (p < 0.05). Specifically, Cu (II) exposure led to reduced nuclear size, increased immature erythrocytes, and a higher percentage of abnormal erythrocytes. Although metamorphic growth parameters did not differ significantly among treatments (p > 0.05), Cu (II) exposure accelerated skin pigmentation and induced tail abnormalities, including tumor-like formations. These findings demonstrate that Cu (II) is toxic to the blood cells and induces morphological abnormalities in F. cancrivora tadpoles, highlighting the potential ecological risks of copper contamination in aquatic habitats.
Bioactivity of Endophytic Fungal Extract Isolated from Purun (Eleocharis dulcis (Burm.f.) Trin. ex Hensch) Oktiansyah, Rian; Pratiwi, Mardiyah; Kharisma, Sully Pudja; Fauzan, Ahmad Rizki; Utami, Riza; Amelia, Dea; Noviyanto, Noviyanto; Nasution, Sakinah Salman Ahmad
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.52579

Abstract

The study investigates the bioactivity of endophytic fungi isolated from Eleocharis dulcis (Burm.f.) Trin. ex Hensch., focusing on their potential as alternative sources of bioactive compounds. E. dulcis, a plant commonly found in wetland ecosystems, has traditionally been used for its medicinal properties. However, the massive extraction of the plant may lead to ecological imbalance, prompting the exploration of endophytic fungi as a sustainable alternative. The objective of this research is to isolate, identify, and evaluate the antimicrobial and antioxidant properties of endophytic fungi from Eleocharis dulcis. Endophytic fungi were isolated aseptically, followed by morphological identification. Antioxidant and antimicrobial assays were conducted to assess the bioactivity of the fungal extracts. Antioxidant activity was evaluated using the DPPH method, while antibacterial activity was assessed using the disc paper diffusion method. The results revealed that several isolates (from leaves: LP1 – LP6, from root: RP1 – RP3) exhibited significant antimicrobial activity against Escherichia coli and Staphylococcus aureus, with Penicillium spp. and Cladosporium spp. showing the highest levels of bioactivity. Additionally, all fungal isolates demonstrated very strong antioxidant activity, with most achieving an IC50 below 20 µg/mL. In conclusion, the findings suggest that endophytic fungi from E. dulcis represent a valuable source of bioactive compounds with potential applications in pharmaceuticals. Further research is required to isolate and characterize the active compounds, as well as to evaluate their efficacy through in vivo studies
Does the chick of domestik chicken (Gallus gallus domesticus) in early development would able to witstand the injection of crude LPS? lelono, Asmoro; Laensugi, Agatha Dei Gratia; Arimurti, Sattya
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.53503

Abstract

Preventing bacterial infections in poultry farms is a key aspect of effective disease management strategies. Enhancing innate immunity serves as a vital preventive measure in chicken farming. Lipopolysaccharide (LPS), a fundamental component of the bacterial cell wall, has been successfully utilized to boost the innate immune response in susceptible poultry hosts. This study focused on examining the impact of administering crude LPS from Salmonella typhimurium to a domestic chicken (Gallus gallus domesticus) strain during its early developmental stages up to the pre-maturation phase. Immune responses in the chicks were evaluated by observing changes in body temperature and leukocyte differentiation. The chickens were divided into two groups: control (injected with 0.85% NaCl) and treatment (injected with the LPS). The injections were applied in three different ages 7 days, 24 days, and 41 days. The body temperature and leukocyte differential data were collected following the LPS injection. Results showed a significant difference in body temperature and heterophil at the ages of 24 and 41, indicating an immune response characterized by inflammation and elevated heterophil levels. There was a significant increase in basal temperature during early development in line with chicks' development to maintain their homeostatic system. This study concludes that the chicks demonstrated resilience to the presence of LPS by exhibiting heightened responses. The increasing of heterophils concentration provided strong evidence of their immune capability to overcome bacterial infections
In Silico Study of Genus Marchantia using matK loci for DNA Barcoding Farhan, Abdillah Maulana; Ulum, Fuad Bahrul
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.53527

Abstract

Marchantia is a genus of liverworts that can be found in the Asia-Europe Region. Some of the types that can be found are Marchantia polymorpha subsp. montivagans, Marchantia polymorpha subsp. ruderalis, Marchantia paleacea subsp. diptera. All of these species have different levels of kinship, this is due to differences in their nucleotide structure. The purpose of this study was to identify the intolerance between Marchantia species in genomic studies using the NCBI Database. The method used in this research is the collection of nucleotides on NCBI and then construction with Mega 11 software. The marker used in this study is Maturase K (matK), this is because the marker is very optimal in the identification of a molecular-based species. The results of this study are Marchantia paleacea subsp. diptera matK (MP 58 and MP 54) has the highest level of kinship with a score of 99 (Neighbor-Joining Tree) and 100 (Maximum Likelihood Tree) and the outgroup of this phylogenetic is Dumortiera hirsuta. Keywords: Marchantia, matK, NCBI, Phylogenetic.
Propagation of Dendrobium crumenatum Sw. through In Vitro Seed Culture Using Fertilizer-Enriched and Organic Complex Media Su'udi, Mukhamad; Ziyaroh, Putry Lazimatu
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.53562

Abstract

Dendrobium crumenatum Sw. is one of the epiphytic orchids with high ecological and economic value. This study aims to examine the effect of media containing organic compounds enriched with fertilizers for in vitro propagation of D. crumenatum seeds. This culture medium contains organic materials such as potatoes, bananas, coconut water, charcoal, sucrose, and foliar fertilizer. This medium was designed to provide macro, micro, and growth hormone nutrients that support explant growth. The research procedure included sterilization of orchid seeds, explant sowing, and growth observation for about two months. The results showed that orchid seeds experienced optimal growth in media with the addition of coconut water and other organic compounds. The formulation of organic-based media with the addition of coconut water and other supporting compounds is effective in supporting the in vitro growth of orchids, providing opportunities for efficient propagation of orchid species. Explant growth reached 1-2 cm at the 68th day after sowing, with a fresh green colour as an indicator of optimal physiological conditions. The even distribution of shoots indicates that the media provides sufficient space and nutrients without competition between explants. In sum, simple tissue culture media produced from a mixture of organic materials proved reliable function and suited for growing D. crumenatum orchid seeds to produce plantlets, and into further extent it would support conservation efforts. Key words: Conservation, organic materials, pigeon orchid, simple media, tissue culture

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