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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general.
Arjuna Subject : Umum - Umum
Articles 146 Documents
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ENVIRONMENTAL ENHANCEMENT INCREASED SPATIAL MEMORY BUT NO SINGNIFICANT EFFECT ON ANXIETY IN RAT WITH PROPYLTHIOURACIL INDUCTION Noor, Zulkhah; Puspitasari, Dewi Alfika; Adiyani, Oktavia
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.1032

Abstract

This study aims to determine the effect of environmental enrichment on spatial memory and anxiety in Propylthiouracil (PTU)-induced thyroid disorder rats. The design of this study was an experimental post test and controlled group design to analyze differences in spatial memory in rats at the end of the study. The subjects in this study were normal rat pups and Rat with PTU induction. Subjects were divided into 4 groups namely, the control group, the normal group with environmental enrichment, the rat group with PTU induction, and the rat group with PTU induction with environmental enrichment. Each group consisted of 8 rats. At the time the rats were born, the rats were given 0.025% PTU which was mixed in their drink and given to the mother until the rats were born until they were 4 weeks old. Environmental enrichment was carried out for 6 weeks. The spatial memory test in Rat was carried out when the pups were 60 days old. Data analysis used One Way Annova, Kruskal Wallis, Post Hoc Test and Mann-Whitney Test. Enrichment of the environment increased the learning latency time of normal rats by 27.8 ± 1.509 seconds to 9.9 ± 4.434 seconds with memory retention increasing by 6.85% (p <0.05). Environmental enrichment in PTU-induced rats accelerated from 84.5 ± 8.70 to 77 ± 1.383 with memory retention increasing by 7.06% (p <0.05). Environmental enrichment did not significantly affect the behavior and anxiety of normal rats or PTU induced (p>0.05). Environmental enrichment to normal rats and impaired thyroid function can increase spatial memory but has no effect on reducing anxiety.
MUTATION OF LIPASE-PRODUCING BACTERIAL ISOLATE FROM PALM OIL EFFLUENT FOR FAT HYDROLYSIS ON POME dadang suhendar; Ika Rahmatul Layly; Gabriela Christy Sabbathini; Deden Rosid Waltam; Edi Wahjono; Catur Sriherwanto; Haniyya Haniyya
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Lipase is applied to hydrolyzethe residual oil or fat in palm oil mill effluent (POME), which serves as a feedstock for biogas production. A bacterial isolate (Bacillus velezensis) exhibiting lipase activity, previously obtained from Malimping, Pandeglang, Banten, Indonesia, underwentsequential treatments of gamma irradiation (1, 2, and 3 kGy), EMS (ethyl methane sulfonate) mutagenesis (0.5% v/v), and NTG (N-methyl N-nitrosoguanidine) (1 mg mL-1). Following each mutation, lipase activities of the mutant colonies were measured and compared to the wild-type strain. The results revealed that all mutated B. velezensiscolonies exhibited lipase activity values (7.78 ± 0.80 –9.05 ± 0.23 U mL-1) that were not significantly different from the wild-type isolate (8.31 ± 0.01 U mL-1), indicating strong bacterial resistance against the mutagenic treatments. The crude lipase preparation effectively hydrolyzed POME, resulting in a reduction of the COD value (from 131,450 ppm to 88,450 ppm) and O&G content (from 41,400 mg L-1to 5,770 mg L-1) within a 72-hour timeframe.
OPTIMIZATION OF PRIMARY SOMATIC EMBRYOGENESIS INDUCTION OFCOCOA (Theobroma cacaoL.) THROUGH THE ADDITION OF PHENOLICCOMPOUNDSUPPRESSANT MATERIALS Priandani, Putri Oktaviana; Pancaningtyas, Sulistyani; Ubaidillah, Mohammad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.1701

Abstract

Somatic embryogenesis is a propagation technique in which explants form somatic embryos. The percentage of primary somatic embryo formation in cocoa tends to be low due to the significant secretion of phenolic components in flower explants, thereby reducing the conversion rate of explants into embryos. This is indicated by browning that occurs in explants or the culture medium. The purpose of this study was to optimize primary somatic embryogenesis induction in cocoa through the addition of several types of phenolic compound suppressor materials to the culture medium. Staminodia explants were grownon DKW medium with factorial treatment: various phenolic compound suppressing materials (control; 300 mg L-1PVP; 15 mg L-1silver nitrate; 12 mg L-1melatonin; 300 mg L-1ascorbic acid) and cocoa cultivars (MCC-02 and ICCRI-09). The results showed that each material treatment produced a different response in suppressing phenolic compounds in each cultivar. The melatonin treatment resulted in the lowest percentage of browning intensity, which also had the best impact on morphology, callus size, wet weight,and the percentage of live explants.
SYRINGIC ACID AND PHENAZINE PRODUCED BY AN ENDOPHYTIC Pseudomonas aeruginosa STRAIN G-111-0317 AND THEIR ACTIVITIES AGAINST Ganoderma boninense Rupaedah, Bedah; Nurlaila, Nurlaila; Lutfia, Anisa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.1703

Abstract

Syringic acid and phenazine possess antibiotic and antifungal properties, and have demonstrated effectiveness in inhibiting the colonization of Ganoderma boninense on oil palm plants. Some bacteria, including Pseudomonas aeruginosa strain G-111-0317, are capable of producing syringic acid and phenazine. In this study, the culture extract of this bacterial strain was obtained from healthy oil palm plants growing in G. boninense-infected areas at Oil Plant Plantation Pematang Siantar, North Sumatra. The strain was cultured on Nutrient Broth (NB) medium, and the resulting culture filtrate was extracted using ethyl acetate (EtOAc) and concentrated under vacuum. The putative compounds were identified by LC-MS, employing syringic acid and phenazine as reference standards. Antifungal activity against G. boninense in vitro was observed in the EtOAc extract obtained after 8 hours and 24 hours of fermentation, with the 8-hour extract demonstrating the highest activity. These compounds hold promising potential as active agents in inhibiting basal stem rot disease in oil palm plants.
A REVIEW OF NANOCELLULOSE SYNTHESIS METHODS AND ITS APPLICATION Joko Waluyo
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Nanocellulose is a type of cellulose that is being widely developed to replace petroleum-based polymers. This material possesses biocompatible properties, is abundant in nature, and is eco-friendly due to its biodegradability, sustainability, and non-toxic nature. Various nanocellulose synthesis methods are employed, including acid hydrolysis, alkaline, mechanical and biological treatments, as well as ionic liquid and deep eutectic solvent methods. The choice of synthesis method greatly influences the particle size and crystallinity of the resulting nanocellulose; hence further investigation is needed to determine the effectiveness of these methods. Nanocellulose finds applications in various fields, such as films, polymers, cosmetics, medical fuels, and energy storage. Among the different nanocellulose synthesis methods, ionic liquid and deep eutectic solvent (DES) methods have environmentally safe waste with better temperature, time, and diameter control compared to other methods. However, the DES method is currently preferred over the ionic liquid method due to the possibility of separating the lignin waste from the solvent.
ROOT INDUCTION ON THE SHOOTS OF RUBBER TREE (Hevea brasiliensis) CLONE PB 260 THROUGH EX VITRO TECHNIQUE Yusuf Sigit Ahmad Fauzan
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Rubber tree (Hevea brasiliensis) is a crucial plantation commodity for Indonesia's national development. However, the country's rubber productivity lags behind that of other rubber-producing nations. To address this, propagating rubber plants from superior clones through ex-vitro techniques becomes essential.. This study aimed to determine the optimal concentrations of IBA and NAA for root induction in rubber tree shoot explants. A factorial complete randomized design was employed, with the first factor being IBA concentration (0, 100, 200, 300, 400, and 500 ppm) and the second factor being NAA concentration (0, 50, 100, and 150 ppm). The results revealed that the percentage of surviving explants after induction with IBA and NAA did not significantly differ. The rooted explants were obtained using the following treatments: IBA 200 ppm and NAA 0 ppm, IBA 200 ppm and NAA 50 ppm, IBA 300 ppm and NAA ppm, and IBA 400 ppm and NAA 0 ppm. Various concentrations of IBA and NAA did not significantly affect root formation in rubber tree shoot explants.
MUTATION DETECTION OF MULTIDRUG-RESISTANT TUBERCULOSIS BY RT-PCR METHOD AS THE DIAGNOSTIC TOOL OF MDR-TB Sabar Pambudi
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Eight percent of tuberculosis (TB) cases worldwide are resistant to rifampicin, with mutations occurring in the rpoB and katG genes. It is necessary to develop a specific multidrug-resistant (MDR) diagnostic technique using the RT-PCR method in Indonesia to aid in rapid and accurate diagnosis. In-silico testing using SnapGene software resulted in the design of DNA primers for the katG and rpoB genes, plasmids, and specific probes. This study employed a cross-sectional design using 30 non-MDR-TB and MDR-TB samples from RSUD Sitanala, Tangerang Banten, which were tested for amplification of the katG and rpoB genes using Sybr green RT-PCR. Validity testing was conducted using specific probes for the katG and rpoB genes. The amplification results showed that MDR-TB samples and MDR-TB plasmids required a longer time compared to non-MDR-TB samples and non-MDR-TB plasmids. The Quantification Cycle (Cq) value in non-MDR-TB samples was lower than the Cq value in MDR-TB samples. A t-test revealed a significant difference in Cq values of the rpoB and katG genes between MDR-TB and non-MDR-TB patients (p-value < 0.005). These differences in Cq values indicate that the findings of this study can serve as an initial reference for the development of an RT-PCR-based diagnostic kit for MDR-TB.
OPTIMIZATION OF INTRANASAL COVID-19 VACCINE FORMULATION WITH Lactococcus lactis pNZ HCR BACTERIA AS VECTOR IN LIQUID AEROSOL PREPARATION Valentina Yurina
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Vaccination is an effective method to suppress COVID-19 transmission, but injection-based vaccination is less effective due to its inability to induce mucosal immunity. This study aimed to determine the effects of vaccine formulations on bacteria viability and antigen expression to find the optimal formulation. Three intranasal preparation formulations (F1, F2, and F3) were created with different ingredient compositions, along with a control. Physicochemical tests were conducted on day 0 and day 14 to assess bacterial viability, and antigen expression was evaluated using the western blot method. Formula 2, containing sodium alginate (0.615%), trehalose (4.125%), polyvinyl alcohol (0.1%), and calcium chloride (5%), exhibited the best viability test results, although no significant differences were observed among the groups. The study concluded that variations in composition concentrations could affect bacterial stability, with Formula 2 showing the best results in terms of bacteria viability and antigen expression up to 14 days after formulation.
ISOLATION OF BACTERIA AS A BIOREMEDIATION AGENT FOR RECLAMATION OF MERCURY-CONTAMINATED SOILS Muhammad Idris; Rizki Amelia Nasution; Ulfayani Mayasari
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Gold mining commonly involves the use of mercury, which generates hazardous and toxic mercury-based waste, leading to soil contamination and adverse effects on human health. Bioremediation has been explored as a potential solution to this issue. This study aimed to identify indigenous microbial species with potential as bioremediation agents, assess their ability to influence chemical properties, and evaluate their effectiveness in reducing mercury content. The research was conducted in five stages: isolation of native bacteria, testing the potential of microorganisms, assessing the ability to reduce mercury, conducting soil tests, and identifying bacterial characteristics through microscopic and biochemical analyses. The results revealed three types of indigenous microbes, namely Pseudomonas, Neisseria, and Klebsiella bacteria, with the highest potential as bioremediation agents. These bacterial isolates were found to enhance the availability of phosphorus in the soil, maintain soil pH, but had no effect on total soil nitrogen. Furthermore, the bacterial isolates exhibited the ability to reduce mercury content after treatment with NA isolates.
ENZYMATIC DEGUMMING USING XYLANASE AND PECTINASE TO IMPROVE BRIGHTNESS AND FINENESS QUALITY OF RAMIE FIBER (Boehmeria nivea L.) AS TEXTILE RAW MATERIAL Kharis Yohan Abidin; Niknik Nurhayati; Dewi Nandyawati; Gabriela Christy Sabbathini
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Ramie (Boehmeria nivea L.) fiber is an alternative to cotton fiber, and the degumming process is crucial for preparing it as a textile raw material. This study investigates the enzymatic degumming of ramie fiber using a combination of xylanase enzyme from Bacillus halodurans CM1 and commercial pectinase enzyme. The objective is to assess the impact of enzymatic degumming on the physical properties (weight loss, whiteness index, tenacity, elongation, and fineness) of ramie fiber. The degumming process was conducted in a shaker incubator at a temperature of 50 °C, pH 9, and 150 rpm. The degumming treatment involved 3% v/v xylanase, 1% v/v pectinase, 1% v/v surfactant, and 0.05% v/v NaClO2. The results show that the bleaching treatment (S6) resulted in higher fiber weight loss (9.52%), whiteness index (87.87%), tenacity (20.08 g/Tex), and fineness (1.05 denier) compared to the non-bleaching treatment.

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